Differential Activation Profiles of Multiple Transcription Factors During Dendritic Cell Maturation
Immature dendritic cells (DC) at the environmental interfaces, such as the skin, constantly survey the tissue for the emergence of microbial products and pro-inflammatory mediators. Upon recognition of such “danger” signals, they undergo dynamic reprogramming of gene expression and functions, the pr...
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creator | Mizumoto, Norikatsu Hui, Francis Edelbaum, Dale Ryan Weil, M. Wren, Jonathan D. Shalhevet, David Matsue, Hiroyuki Liu, Lei Garner, Harold R. Takashima, Akira |
description | Immature dendritic cells (DC) at the environmental interfaces, such as the skin, constantly survey the tissue for the emergence of microbial products and pro-inflammatory mediators. Upon recognition of such “danger” signals, they undergo dynamic reprogramming of gene expression and functions, the process known as DC maturation, which plays critical roles in both innate and adaptive immune responses. Although DC have been shown to discriminate different maturation stimuli by expressing stimulus-specific signature genes and unique phenotypic and functional properties, underlying mechanisms for this extraordinary plasticity remain relatively unclear. We hypothesized that DC might activate unique sets of transcription factors (TF) upon sensing different stimuli. To test this hypothesis, we transduced a mouse epidermal-derived DC line XS106 to express the luciferase reporter gene under the control of each of 15 different cis-enhancer elements. The resulting DC panels were then exposed to 14 different microbial, endogenous, environmental, and pharmacological agents that produced unique maturational changes. This approach allowed systematic determination of TF activation profiles in DC. Our results revealed striking diversity, with different classes of stimuli triggering preferential activation of distinct sets of TF. We propose that differential TF usage represents a previously unrecognized mechanism regulating the direction of DC maturation. |
doi_str_mv | 10.1111/j.0022-202X.2005.23616.x |
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Upon recognition of such “danger” signals, they undergo dynamic reprogramming of gene expression and functions, the process known as DC maturation, which plays critical roles in both innate and adaptive immune responses. Although DC have been shown to discriminate different maturation stimuli by expressing stimulus-specific signature genes and unique phenotypic and functional properties, underlying mechanisms for this extraordinary plasticity remain relatively unclear. We hypothesized that DC might activate unique sets of transcription factors (TF) upon sensing different stimuli. To test this hypothesis, we transduced a mouse epidermal-derived DC line XS106 to express the luciferase reporter gene under the control of each of 15 different cis-enhancer elements. The resulting DC panels were then exposed to 14 different microbial, endogenous, environmental, and pharmacological agents that produced unique maturational changes. This approach allowed systematic determination of TF activation profiles in DC. Our results revealed striking diversity, with different classes of stimuli triggering preferential activation of distinct sets of TF. We propose that differential TF usage represents a previously unrecognized mechanism regulating the direction of DC maturation.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1111/j.0022-202X.2005.23616.x</identifier><identifier>PMID: 15816829</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Differentiation - immunology ; dendritic cell ; Dendritic Cells - cytology ; Dendritic Cells - physiology ; Gene Expression Profiling ; immunity ; langerhans cell ; Luciferases - genetics ; Mice ; Mice, Inbred A ; Phenotype ; Signal Transduction - immunology ; Stimulation, Chemical ; transcription factor ; Transcription Factors - genetics ; Transcription Factors - immunology</subject><ispartof>Journal of investigative dermatology, 2005-04, Vol.124 (4), p.718-724</ispartof><rights>2005 The Society for Investigative Dermatology, Inc</rights><rights>Copyright Nature Publishing Group Apr 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-cdd2b86f8ff7c51fce28eaa73ebc10b2f3507cf07eea3f85a7229b7e5fcd40aa3</citedby><cites>FETCH-LOGICAL-c471t-cdd2b86f8ff7c51fce28eaa73ebc10b2f3507cf07eea3f85a7229b7e5fcd40aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/210371052?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,64390,64392,64394,72474</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15816829$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mizumoto, Norikatsu</creatorcontrib><creatorcontrib>Hui, Francis</creatorcontrib><creatorcontrib>Edelbaum, Dale</creatorcontrib><creatorcontrib>Ryan Weil, M.</creatorcontrib><creatorcontrib>Wren, Jonathan D.</creatorcontrib><creatorcontrib>Shalhevet, David</creatorcontrib><creatorcontrib>Matsue, Hiroyuki</creatorcontrib><creatorcontrib>Liu, Lei</creatorcontrib><creatorcontrib>Garner, Harold R.</creatorcontrib><creatorcontrib>Takashima, Akira</creatorcontrib><title>Differential Activation Profiles of Multiple Transcription Factors During Dendritic Cell Maturation</title><title>Journal of investigative dermatology</title><addtitle>J Invest Dermatol</addtitle><description>Immature dendritic cells (DC) at the environmental interfaces, such as the skin, constantly survey the tissue for the emergence of microbial products and pro-inflammatory mediators. Upon recognition of such “danger” signals, they undergo dynamic reprogramming of gene expression and functions, the process known as DC maturation, which plays critical roles in both innate and adaptive immune responses. Although DC have been shown to discriminate different maturation stimuli by expressing stimulus-specific signature genes and unique phenotypic and functional properties, underlying mechanisms for this extraordinary plasticity remain relatively unclear. We hypothesized that DC might activate unique sets of transcription factors (TF) upon sensing different stimuli. To test this hypothesis, we transduced a mouse epidermal-derived DC line XS106 to express the luciferase reporter gene under the control of each of 15 different cis-enhancer elements. The resulting DC panels were then exposed to 14 different microbial, endogenous, environmental, and pharmacological agents that produced unique maturational changes. This approach allowed systematic determination of TF activation profiles in DC. Our results revealed striking diversity, with different classes of stimuli triggering preferential activation of distinct sets of TF. We propose that differential TF usage represents a previously unrecognized mechanism regulating the direction of DC maturation.</description><subject>Animals</subject><subject>Cell Differentiation - immunology</subject><subject>dendritic cell</subject><subject>Dendritic Cells - cytology</subject><subject>Dendritic Cells - physiology</subject><subject>Gene Expression Profiling</subject><subject>immunity</subject><subject>langerhans cell</subject><subject>Luciferases - genetics</subject><subject>Mice</subject><subject>Mice, Inbred A</subject><subject>Phenotype</subject><subject>Signal Transduction - immunology</subject><subject>Stimulation, Chemical</subject><subject>transcription factor</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - immunology</subject><issn>0022-202X</issn><issn>1523-1747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqFkUGP1CAYhonRuOPoT9AQD95agRboHtcZV012o4c18Ubo1w_DpAMj0M36721nJmviRS4ceL6XN89HCOWs5vN5v6sZE6ISTPyoBWOyFo3iqn54QlZciqbiutVPyeoRuiAvct4xxlUru-fkgsuOq05crghsvXOYMBRvR3oFxd_b4mOg31J0fsRMo6O301j8YUR6l2zIkPzhiFxbKDFlup2SDz_pFsOQfPFANziO9NaWKR2zXpJnzo4ZX53vNfl-_fFu87m6-frpy-bqpoJW81LBMIi-U65zToPkDlB0aK1usAfOeuEayTQ4phFt4zpptRCXvUbpYGiZtc2avDvlHlL8NWEuZu8zzF1swDhlo7QWvGnVDL79B9zFKYW5mxGcNZqzWeKadCcIUsw5oTOH5Pc2_TacmWULZmcWwWYRbJYtmOMWzMM8-uacP_V7HP4OnrXPwOsTEBZJ-AhIxYRWy98fTu8467r3mEwGjwFw8AmhmCH6_7f4AwIkpSg</recordid><startdate>20050401</startdate><enddate>20050401</enddate><creator>Mizumoto, Norikatsu</creator><creator>Hui, Francis</creator><creator>Edelbaum, Dale</creator><creator>Ryan Weil, M.</creator><creator>Wren, Jonathan D.</creator><creator>Shalhevet, David</creator><creator>Matsue, Hiroyuki</creator><creator>Liu, Lei</creator><creator>Garner, Harold R.</creator><creator>Takashima, Akira</creator><general>Elsevier Inc</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050401</creationdate><title>Differential Activation Profiles of Multiple Transcription Factors During Dendritic Cell Maturation</title><author>Mizumoto, Norikatsu ; 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subjects | Animals Cell Differentiation - immunology dendritic cell Dendritic Cells - cytology Dendritic Cells - physiology Gene Expression Profiling immunity langerhans cell Luciferases - genetics Mice Mice, Inbred A Phenotype Signal Transduction - immunology Stimulation, Chemical transcription factor Transcription Factors - genetics Transcription Factors - immunology |
title | Differential Activation Profiles of Multiple Transcription Factors During Dendritic Cell Maturation |
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