Analyzing the Degradation of PERIOD Protein by the Ubiquitin–Proteasome Pathway in Cultured Drosophila Cells

Time-of-day specific changes in the levels of key clock proteins are critical for the normal progression of circadian pacemakers. Evidence indicates a major role for the ubiquitin–proteasome pathway (UPP) in the temporal control of clock protein stability. A conserved feature of animal clocks is tha...

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Veröffentlicht in:	Methods in Enzymology 2005, Vol.393, p.394-408
Hauptverfasser:	Ko, Hyuk Wan, Edery, Isaac
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cells, Cultured Drosophila Drosophila melanogaster - metabolism Drosophila Proteins Nuclear Proteins - metabolism Period Circadian Proteins Proteasome Endopeptidase Complex - metabolism Proteasome Inhibitors Transfection - methods Ubiquitin - metabolism
Online-Zugang:	Volltext
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