Nitric Oxide Synthase (NOS) Characterization in Leishmania amazonensis Axenic Amastigotes
Although Leishmania virulence may be modulated by environmental and genetic factors of their mammalian hosts and sand fly vectors, molecular determinants of Leishmania sp. are the key elements. This work evidences that Leishmania amazonensis axenic amastigotes produce comparatively more NO than infe...
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| Veröffentlicht in: | Archives of medical research 2006-04, Vol.37 (3), p.328-333 |
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| container_title | Archives of medical research |
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| creator | Genestra, Marcelo Guedes-Silva, Damiana Souza, Wilson J.S. Cysne-Finkelstein, Léa Soares-Bezerra, Rômulo José Monteiro, Fabiane Pereira Leon, Leonor L. |
| description | Although
Leishmania virulence may be modulated by environmental and genetic factors of their mammalian hosts and sand fly vectors, molecular determinants of
Leishmania sp. are the key elements. This work evidences that
Leishmania amazonensis axenic amastigotes produce comparatively more NO than infective promastigotes.
A soluble NOS was purified from
L. amazonensis axenic amastigotes by affinity chromatography (2′,5′-ADP-agarose), and on SDS-PAGE the enzyme migrates as a single protein band.
The presence of a constitutive NOS was detected through immunofluorescence using antibody against neuronal NOS (nNOS) and in NADPH consumption assays.
The present data show that NOS is prominent in axenic amastigote preparations, suggesting an association with the infectivity and/or an escaping mechanism of the parasite. The relationship between the NO-generating systems in the parasite and in their host cell warrants further investigation. |
| doi_str_mv | 10.1016/j.arcmed.2005.07.011 |
| format | Article |
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Leishmania virulence may be modulated by environmental and genetic factors of their mammalian hosts and sand fly vectors, molecular determinants of
Leishmania sp. are the key elements. This work evidences that
Leishmania amazonensis axenic amastigotes produce comparatively more NO than infective promastigotes.
A soluble NOS was purified from
L. amazonensis axenic amastigotes by affinity chromatography (2′,5′-ADP-agarose), and on SDS-PAGE the enzyme migrates as a single protein band.
The presence of a constitutive NOS was detected through immunofluorescence using antibody against neuronal NOS (nNOS) and in NADPH consumption assays.
The present data show that NOS is prominent in axenic amastigote preparations, suggesting an association with the infectivity and/or an escaping mechanism of the parasite. The relationship between the NO-generating systems in the parasite and in their host cell warrants further investigation.</description><identifier>ISSN: 0188-4409</identifier><identifier>EISSN: 1873-5487</identifier><identifier>DOI: 10.1016/j.arcmed.2005.07.011</identifier><identifier>PMID: 16513480</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Axenic amastigotes ; Leishmania - classification ; Leishmania - enzymology ; Leishmania - growth & development ; Leishmania amazonensis ; Nitric oxide ; Nitric Oxide - biosynthesis ; Nitric oxide synthase ; Nitric Oxide Synthase - metabolism</subject><ispartof>Archives of medical research, 2006-04, Vol.37 (3), p.328-333</ispartof><rights>2006 IMSS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-51483434b7e367e7e700cff4b6c81f0dd633dc1d3542cc1a88e4b35de7e384913</citedby><cites>FETCH-LOGICAL-c360t-51483434b7e367e7e700cff4b6c81f0dd633dc1d3542cc1a88e4b35de7e384913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0188440905003115$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16513480$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Genestra, Marcelo</creatorcontrib><creatorcontrib>Guedes-Silva, Damiana</creatorcontrib><creatorcontrib>Souza, Wilson J.S.</creatorcontrib><creatorcontrib>Cysne-Finkelstein, Léa</creatorcontrib><creatorcontrib>Soares-Bezerra, Rômulo José</creatorcontrib><creatorcontrib>Monteiro, Fabiane Pereira</creatorcontrib><creatorcontrib>Leon, Leonor L.</creatorcontrib><title>Nitric Oxide Synthase (NOS) Characterization in Leishmania amazonensis Axenic Amastigotes</title><title>Archives of medical research</title><addtitle>Arch Med Res</addtitle><description>Although
Leishmania virulence may be modulated by environmental and genetic factors of their mammalian hosts and sand fly vectors, molecular determinants of
Leishmania sp. are the key elements. This work evidences that
Leishmania amazonensis axenic amastigotes produce comparatively more NO than infective promastigotes.
A soluble NOS was purified from
L. amazonensis axenic amastigotes by affinity chromatography (2′,5′-ADP-agarose), and on SDS-PAGE the enzyme migrates as a single protein band.
The presence of a constitutive NOS was detected through immunofluorescence using antibody against neuronal NOS (nNOS) and in NADPH consumption assays.
The present data show that NOS is prominent in axenic amastigote preparations, suggesting an association with the infectivity and/or an escaping mechanism of the parasite. The relationship between the NO-generating systems in the parasite and in their host cell warrants further investigation.</description><subject>Animals</subject><subject>Axenic amastigotes</subject><subject>Leishmania - classification</subject><subject>Leishmania - enzymology</subject><subject>Leishmania - growth & development</subject><subject>Leishmania amazonensis</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Nitric oxide synthase</subject><subject>Nitric Oxide Synthase - metabolism</subject><issn>0188-4409</issn><issn>1873-5487</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMoWj_-gcieRA-7Tkw2iRehFL-g2IN68BTSZNamdLOapKL-ere0eJQ5zGGeeYd5CDmmUFGg4mJemWhbdNUlQF2BrIDSLTKgSrKy5kpukwFQpUrO4WqP7Kc0BwDFhdwle1TUlHEFA_L66HP0tph8eYfF03fIM5OwOHucPJ0Xo5mJxmaM_sdk34XCh2KMPs1aE7wpTGt-uoAh-VQMvzD0McPWpOzfuozpkOw0ZpHwaNMPyMvtzfPovhxP7h5Gw3FpmYBc1pQrxhmfSmRCYl8Atmn4VFhFG3BOMOYsdazml9ZSoxTyKatdTzLFryg7IKfr3PfYfSwxZd36ZHGxMAG7ZdJCSlpLED3I16CNXUoRG_0efWvit6agV0r1XK-V6pVSDVL3Svu1k03-crqa_S1tHPbA9RrA_stPj1En6zFYdD6izdp1_v8Lv_m0iWM</recordid><startdate>20060401</startdate><enddate>20060401</enddate><creator>Genestra, Marcelo</creator><creator>Guedes-Silva, Damiana</creator><creator>Souza, Wilson J.S.</creator><creator>Cysne-Finkelstein, Léa</creator><creator>Soares-Bezerra, Rômulo José</creator><creator>Monteiro, Fabiane Pereira</creator><creator>Leon, Leonor L.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060401</creationdate><title>Nitric Oxide Synthase (NOS) Characterization in Leishmania amazonensis Axenic Amastigotes</title><author>Genestra, Marcelo ; Guedes-Silva, Damiana ; Souza, Wilson J.S. ; Cysne-Finkelstein, Léa ; Soares-Bezerra, Rômulo José ; Monteiro, Fabiane Pereira ; Leon, Leonor L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-51483434b7e367e7e700cff4b6c81f0dd633dc1d3542cc1a88e4b35de7e384913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Axenic amastigotes</topic><topic>Leishmania - classification</topic><topic>Leishmania - enzymology</topic><topic>Leishmania - growth & development</topic><topic>Leishmania amazonensis</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Nitric oxide synthase</topic><topic>Nitric Oxide Synthase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Genestra, Marcelo</creatorcontrib><creatorcontrib>Guedes-Silva, Damiana</creatorcontrib><creatorcontrib>Souza, Wilson J.S.</creatorcontrib><creatorcontrib>Cysne-Finkelstein, Léa</creatorcontrib><creatorcontrib>Soares-Bezerra, Rômulo José</creatorcontrib><creatorcontrib>Monteiro, Fabiane Pereira</creatorcontrib><creatorcontrib>Leon, Leonor L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of medical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Genestra, Marcelo</au><au>Guedes-Silva, Damiana</au><au>Souza, Wilson J.S.</au><au>Cysne-Finkelstein, Léa</au><au>Soares-Bezerra, Rômulo José</au><au>Monteiro, Fabiane Pereira</au><au>Leon, Leonor L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitric Oxide Synthase (NOS) Characterization in Leishmania amazonensis Axenic Amastigotes</atitle><jtitle>Archives of medical research</jtitle><addtitle>Arch Med Res</addtitle><date>2006-04-01</date><risdate>2006</risdate><volume>37</volume><issue>3</issue><spage>328</spage><epage>333</epage><pages>328-333</pages><issn>0188-4409</issn><eissn>1873-5487</eissn><abstract>Although
Leishmania virulence may be modulated by environmental and genetic factors of their mammalian hosts and sand fly vectors, molecular determinants of
Leishmania sp. are the key elements. This work evidences that
Leishmania amazonensis axenic amastigotes produce comparatively more NO than infective promastigotes.
A soluble NOS was purified from
L. amazonensis axenic amastigotes by affinity chromatography (2′,5′-ADP-agarose), and on SDS-PAGE the enzyme migrates as a single protein band.
The presence of a constitutive NOS was detected through immunofluorescence using antibody against neuronal NOS (nNOS) and in NADPH consumption assays.
The present data show that NOS is prominent in axenic amastigote preparations, suggesting an association with the infectivity and/or an escaping mechanism of the parasite. The relationship between the NO-generating systems in the parasite and in their host cell warrants further investigation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16513480</pmid><doi>10.1016/j.arcmed.2005.07.011</doi><tpages>6</tpages></addata></record> |
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| ispartof | Archives of medical research, 2006-04, Vol.37 (3), p.328-333 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Axenic amastigotes Leishmania - classification Leishmania - enzymology Leishmania - growth & development Leishmania amazonensis Nitric oxide Nitric Oxide - biosynthesis Nitric oxide synthase Nitric Oxide Synthase - metabolism |
| title | Nitric Oxide Synthase (NOS) Characterization in Leishmania amazonensis Axenic Amastigotes |
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