Effect of Sex and Assay Method on Serum Concentrations of Growth Hormone in Patients with Acromegaly and in Healthy Controls
Diagnosis and follow-up of acromegaly is based on measurements of serum growth hormone (GH) concentrations during an oral glucose tolerance test (OGTT). A nadir value
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| Veröffentlicht in: | Clinical chemistry (Baltimore, Md.) Md.), 2006-03, Vol.52 (3), p.468-473 |
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| container_title | Clinical chemistry (Baltimore, Md.) |
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| creator | Markkanen, Helene Pekkarinen, Tuula Valimaki, Matti J Alfthan, Henrik Kauppinen-Makelin, Ritva Sane, Timo Stenman, Ulf-Hakan |
| description | Diagnosis and follow-up of acromegaly is based on measurements of serum growth hormone (GH) concentrations during an oral glucose tolerance test (OGTT). A nadir value |
| doi_str_mv | 10.1373/clinchem.2005.060236 |
| format | Article |
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To compare the results and subsequent patient classification obtained with 3 GH assays, we obtained basal serum samples from 78 apparently healthy adult controls (43 women and 35 men; median age, 32.5 years) and from 71 treated (44 women and 27 men; median age, 55.2 years) and 7 untreated acromegaly patients (4 women and 3 men; median age, 54.6 years), and OGTT was performed on all patients and on 72 of the 78 controls. GH was determined by 2 immunometric assays-a double monoclonal (AutoDELFIA; Wallac) and a monopolyclonal (Immulite 2000; DPC) assay-and in a limited set of samples by an RIA (Spectria RIA; Orion).
There was a strong correlation (r = 0.995; P < 0.001) between the 2 immunometric methods, but the results obtained with the Immulite 2000 were, on average, 1.4-fold higher than those obtained with the AutoDELFIA. At concentrations around the cutoff (1 microg/L), however, the difference was approximately 2-fold. Overall, the Orion RIA method also showed a good correlation (r = 0.951-0.959) with the other methods, but it did not measure concentrations <2 microg/L. Women had higher basal and OGTT nadir GH concentrations than men.
Reference intervals should be determined separately for each method, and the need for establishing sex-specific reference values should be investigated.</description><identifier>ISSN: 0009-9147</identifier><identifier>EISSN: 1530-8561</identifier><identifier>DOI: 10.1373/clinchem.2005.060236</identifier><identifier>PMID: 16439607</identifier><identifier>CODEN: CLCHAU</identifier><language>eng</language><publisher>Washington, DC: Am Assoc Clin Chem</publisher><subject>Acromegaly - blood ; Adult ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Female ; Fundamental and applied biological sciences. Psychology ; Human Growth Hormone - blood ; Humans ; Immunoassay - methods ; Investigative techniques, diagnostic techniques (general aspects) ; Male ; Medical sciences ; Middle Aged ; Reference Values ; Serum ; Sex Factors</subject><ispartof>Clinical chemistry (Baltimore, Md.), 2006-03, Vol.52 (3), p.468-473</ispartof><rights>2006 INIST-CNRS</rights><rights>Copyright American Association for Clinical Chemistry Mar 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-8e8f320fd82b34634997a8e29e10e42109b0d6191e71adbe4a951439890d76713</citedby><cites>FETCH-LOGICAL-c438t-8e8f320fd82b34634997a8e29e10e42109b0d6191e71adbe4a951439890d76713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17567456$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16439607$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Markkanen, Helene</creatorcontrib><creatorcontrib>Pekkarinen, Tuula</creatorcontrib><creatorcontrib>Valimaki, Matti J</creatorcontrib><creatorcontrib>Alfthan, Henrik</creatorcontrib><creatorcontrib>Kauppinen-Makelin, Ritva</creatorcontrib><creatorcontrib>Sane, Timo</creatorcontrib><creatorcontrib>Stenman, Ulf-Hakan</creatorcontrib><title>Effect of Sex and Assay Method on Serum Concentrations of Growth Hormone in Patients with Acromegaly and in Healthy Controls</title><title>Clinical chemistry (Baltimore, Md.)</title><addtitle>Clin Chem</addtitle><description>Diagnosis and follow-up of acromegaly is based on measurements of serum growth hormone (GH) concentrations during an oral glucose tolerance test (OGTT). A nadir value <1 microg/L is commonly used to define a normal response, but some authors suggest lower cutoff values.
To compare the results and subsequent patient classification obtained with 3 GH assays, we obtained basal serum samples from 78 apparently healthy adult controls (43 women and 35 men; median age, 32.5 years) and from 71 treated (44 women and 27 men; median age, 55.2 years) and 7 untreated acromegaly patients (4 women and 3 men; median age, 54.6 years), and OGTT was performed on all patients and on 72 of the 78 controls. GH was determined by 2 immunometric assays-a double monoclonal (AutoDELFIA; Wallac) and a monopolyclonal (Immulite 2000; DPC) assay-and in a limited set of samples by an RIA (Spectria RIA; Orion).
There was a strong correlation (r = 0.995; P < 0.001) between the 2 immunometric methods, but the results obtained with the Immulite 2000 were, on average, 1.4-fold higher than those obtained with the AutoDELFIA. At concentrations around the cutoff (1 microg/L), however, the difference was approximately 2-fold. Overall, the Orion RIA method also showed a good correlation (r = 0.951-0.959) with the other methods, but it did not measure concentrations <2 microg/L. Women had higher basal and OGTT nadir GH concentrations than men.
Reference intervals should be determined separately for each method, and the need for establishing sex-specific reference values should be investigated.</description><subject>Acromegaly - blood</subject><subject>Adult</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Human Growth Hormone - blood</subject><subject>Humans</subject><subject>Immunoassay - methods</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Reference Values</subject><subject>Serum</subject><subject>Sex Factors</subject><issn>0009-9147</issn><issn>1530-8561</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkUFr3DAQhUVpaLZp_0EpotDevJEsWbKOy5JmCykJtD0LrTyOHWQplWychf74arMugZ4GzfvmMaOH0AdK1pRJdmld720Hw7okpFoTQUomXqEVrRgp6krQ12hFCFGFolyeo7cpPeQnl7V4g86p4EwJIlfoz1Xbgh1xaPEPeMLGN3iTkjng7zB2ocHB536cBrwN3oIfoxn74NORv45hHju8C3EIHnDv8V0WM5Pw3GdhY2MY4N64w7Nt1ndg3Ngdjl5jDC69Q2etcQneL_UC_fp69XO7K25ur79tNzeF5aweixrqlpWkbepyz7hgXClpaigVUAK8pETtSSOooiCpafbAjapoPrBWpJFCUnaBvpx8H2P4PUEa9dAnC84ZD2FKWkiZv60SGfz0H_gQpujzbrqknFBaKpYhfoLyfSlFaPVj7AcTD5oSfYxG_4tGH6PRp2jy2MfFe9oP0LwMLVlk4PMCmGSNa6Pxtk8vnKyE5M9LLlzX33dzH0GnwTiXbame57kqNdNc1OwvQyOlMg</recordid><startdate>20060301</startdate><enddate>20060301</enddate><creator>Markkanen, Helene</creator><creator>Pekkarinen, Tuula</creator><creator>Valimaki, Matti J</creator><creator>Alfthan, Henrik</creator><creator>Kauppinen-Makelin, Ritva</creator><creator>Sane, Timo</creator><creator>Stenman, Ulf-Hakan</creator><general>Am Assoc Clin Chem</general><general>American Association for Clinical Chemistry</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4U-</scope><scope>7QO</scope><scope>7RV</scope><scope>7TM</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>S0X</scope><scope>7X8</scope></search><sort><creationdate>20060301</creationdate><title>Effect of Sex and Assay Method on Serum Concentrations of Growth Hormone in Patients with Acromegaly and in Healthy Controls</title><author>Markkanen, Helene ; Pekkarinen, Tuula ; Valimaki, Matti J ; Alfthan, Henrik ; Kauppinen-Makelin, Ritva ; Sane, Timo ; Stenman, Ulf-Hakan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-8e8f320fd82b34634997a8e29e10e42109b0d6191e71adbe4a951439890d76713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Acromegaly - blood</topic><topic>Adult</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Human Growth Hormone - blood</topic><topic>Humans</topic><topic>Immunoassay - methods</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Reference Values</topic><topic>Serum</topic><topic>Sex Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Markkanen, Helene</creatorcontrib><creatorcontrib>Pekkarinen, Tuula</creatorcontrib><creatorcontrib>Valimaki, Matti J</creatorcontrib><creatorcontrib>Alfthan, Henrik</creatorcontrib><creatorcontrib>Kauppinen-Makelin, Ritva</creatorcontrib><creatorcontrib>Sane, Timo</creatorcontrib><creatorcontrib>Stenman, Ulf-Hakan</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>University Readers</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Markkanen, Helene</au><au>Pekkarinen, Tuula</au><au>Valimaki, Matti J</au><au>Alfthan, Henrik</au><au>Kauppinen-Makelin, Ritva</au><au>Sane, Timo</au><au>Stenman, Ulf-Hakan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Sex and Assay Method on Serum Concentrations of Growth Hormone in Patients with Acromegaly and in Healthy Controls</atitle><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle><addtitle>Clin Chem</addtitle><date>2006-03-01</date><risdate>2006</risdate><volume>52</volume><issue>3</issue><spage>468</spage><epage>473</epage><pages>468-473</pages><issn>0009-9147</issn><eissn>1530-8561</eissn><coden>CLCHAU</coden><abstract>Diagnosis and follow-up of acromegaly is based on measurements of serum growth hormone (GH) concentrations during an oral glucose tolerance test (OGTT). A nadir value <1 microg/L is commonly used to define a normal response, but some authors suggest lower cutoff values.
To compare the results and subsequent patient classification obtained with 3 GH assays, we obtained basal serum samples from 78 apparently healthy adult controls (43 women and 35 men; median age, 32.5 years) and from 71 treated (44 women and 27 men; median age, 55.2 years) and 7 untreated acromegaly patients (4 women and 3 men; median age, 54.6 years), and OGTT was performed on all patients and on 72 of the 78 controls. GH was determined by 2 immunometric assays-a double monoclonal (AutoDELFIA; Wallac) and a monopolyclonal (Immulite 2000; DPC) assay-and in a limited set of samples by an RIA (Spectria RIA; Orion).
There was a strong correlation (r = 0.995; P < 0.001) between the 2 immunometric methods, but the results obtained with the Immulite 2000 were, on average, 1.4-fold higher than those obtained with the AutoDELFIA. At concentrations around the cutoff (1 microg/L), however, the difference was approximately 2-fold. Overall, the Orion RIA method also showed a good correlation (r = 0.951-0.959) with the other methods, but it did not measure concentrations <2 microg/L. Women had higher basal and OGTT nadir GH concentrations than men.
Reference intervals should be determined separately for each method, and the need for establishing sex-specific reference values should be investigated.</abstract><cop>Washington, DC</cop><pub>Am Assoc Clin Chem</pub><pmid>16439607</pmid><doi>10.1373/clinchem.2005.060236</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Clinical chemistry (Baltimore, Md.), 2006-03, Vol.52 (3), p.468-473 |
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| subjects | Acromegaly - blood Adult Analytical, structural and metabolic biochemistry Biological and medical sciences Female Fundamental and applied biological sciences. Psychology Human Growth Hormone - blood Humans Immunoassay - methods Investigative techniques, diagnostic techniques (general aspects) Male Medical sciences Middle Aged Reference Values Serum Sex Factors |
| title | Effect of Sex and Assay Method on Serum Concentrations of Growth Hormone in Patients with Acromegaly and in Healthy Controls |
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