HIV Type 1 Can Act as an APC upon Acquisition from the Host Cell of Peptide-Loaded HLA-DR and CD86 Molecules

It is well documented that a wide range of host-derived cell surface constituents is inserted within HIV type 1 (HIV-1) and located on the exterior of the virion. Although no virus-associated protein of host origin has been shown to be absolutely required for virus replication, studies have revealed...

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Veröffentlicht in:The Journal of immunology (1950) 2005-04, Vol.174 (8), p.4779-4788
Hauptverfasser: Roy, Jocelyn, Martin, Genevieve, Giguere, Jean-Francois, Belanger, Dave, Petrin, Myriam, Tremblay, Michel J
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container_issue 8
container_start_page 4779
container_title The Journal of immunology (1950)
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creator Roy, Jocelyn
Martin, Genevieve
Giguere, Jean-Francois
Belanger, Dave
Petrin, Myriam
Tremblay, Michel J
description It is well documented that a wide range of host-derived cell surface constituents is inserted within HIV type 1 (HIV-1) and located on the exterior of the virion. Although no virus-associated protein of host origin has been shown to be absolutely required for virus replication, studies have revealed that many of these proteins are functional and can affect several steps of the virus life cycle. In this study, we found that HIV-1 acquires peptide-loaded class II MHC (MHC-II) and the costimulatory CD86 molecules from the host cell. Moreover, we present evidence that virions bearing such peptide-loaded MHC-II and CD86 proteins can lead to activation of the transcription factors NF-kappa B and NF-AT in an Ag-specific human T cell line. A linear correlation was found between activation of NF-kappa B and the amount of peptide-loaded MHC-II molecules inserted within HIV-1. Finally, transcription of unintegrated and integrated HIV-1 DNA was promoted upon exposure of peptide-specific human T cells to viruses bearing both peptide-loaded MHC-II and CD86 proteins. These data suggest that HIV-1 can operate as an APC depending on the nature of virus-anchored host cell membrane components. It can be proposed that HIV-1 can manipulate one of its primary targets through the process of incorporation of host-derived proteins.
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source MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Antigen-Presenting Cells - immunology
Antigen-Presenting Cells - virology
Antigens, CD - metabolism
B7-2 Antigen
CD4-Positive T-Lymphocytes - immunology
CD4-Positive T-Lymphocytes - metabolism
CD4-Positive T-Lymphocytes - virology
Cell Line
Genome, Viral
HIV-1 - genetics
HIV-1 - immunology
HIV-1 - pathogenicity
HIV-1 - physiology
HLA-DR Antigens - metabolism
Human immunodeficiency virus 1
Humans
In Vitro Techniques
Membrane Glycoproteins - metabolism
NF-kappa B - metabolism
Secretory Vesicles - immunology
Secretory Vesicles - metabolism
Secretory Vesicles - virology
Transcription, Genetic
title HIV Type 1 Can Act as an APC upon Acquisition from the Host Cell of Peptide-Loaded HLA-DR and CD86 Molecules
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