Coelution of Other Proteins with Albumin during Size-Exclusion HPLC: Implications for Analysis of Urinary Albumin

Size-exclusion HPLC has been used as an alternative to immunoassays for quantifying urinary albumin (microalbumin). Systematically higher values for the HPLC method have been proposed to result from nonimmunoreactive albumin. We evaluated separation of purified proteins and urinary components by siz...

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Veröffentlicht in:	Clinical chemistry (Baltimore, Md.) Md.), 2006-03, Vol.52 (3), p.389-397
Hauptverfasser:	Sviridov, Denis, Meilinger, Bonnie, Drake, Steven K, Hoehn, Gerard T, Hortin, Glen L
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Albuminuria - diagnosis Albuminuria - urine Analytical, structural and metabolic biochemistry Biological and medical sciences Blood Proteins - urine Chromatography Chromatography, High Pressure Liquid False Positive Reactions Fundamental and applied biological sciences. Psychology Glycoproteins Humans Immunoassay Immunoassays Investigative techniques, diagnostic techniques (general aspects) Liquid chromatography Mass spectrometry Medical sciences Proteinase inhibitors Proteins Scientific imaging Serum Albumin - analysis Urine
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