Clinical evaluation of repetitive sequence-based polymerase chain reaction using the Diversi-Lab System for strain typing of vancomycin-resistant enterococci
The reliability of the Diversi-Lab System, an automated method of microbial strain typing using repetitive sequence-based polymerase chain reaction (rep-PCR), was evaluated by comparing results with those obtained by pulsed-field gel electrophoresis (PFGE). Ninety-five clinical isolates of vancomyci...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2006-03, Vol.54 (3), p.183-187 |
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| creator | Pounder, June I Shutt, Cheryl K Schaecher, Barbara J Woods, Gail L |
| description | The reliability of the Diversi-Lab System, an automated method of microbial strain typing using repetitive sequence-based polymerase chain reaction (rep-PCR), was evaluated by comparing results with those obtained by pulsed-field gel electrophoresis (PFGE). Ninety-five clinical isolates of vancomycin-resistant enterococci (VRE; 13 groups, 2-17 isolates per group) sent to Associated Regional and University Pathologists (ARUP) Laboratories for typing were tested by both methods. Rep-PCR and PFGE results were concordant for 83 isolates: all 32 isolates in 6 of the groups and 51 of the 63 isolates in the other 7 groups. Clustering of the remaining 12 isolates differed. With the Diversi-Lab System, analysis is objective, and results are available in 4 h, compared with a more subjective analysis and a 2- to 3-day turnaround time for PFGE. The Diversi-Lab System may be a viable alternative to PFGE for typing VRE in clinical reference laboratories. |
| format | Article |
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Ninety-five clinical isolates of vancomycin-resistant enterococci (VRE; 13 groups, 2-17 isolates per group) sent to Associated Regional and University Pathologists (ARUP) Laboratories for typing were tested by both methods. Rep-PCR and PFGE results were concordant for 83 isolates: all 32 isolates in 6 of the groups and 51 of the 63 isolates in the other 7 groups. Clustering of the remaining 12 isolates differed. With the Diversi-Lab System, analysis is objective, and results are available in 4 h, compared with a more subjective analysis and a 2- to 3-day turnaround time for PFGE. 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| ispartof | Diagnostic microbiology and infectious disease, 2006-03, Vol.54 (3), p.183-187 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Bacterial Typing Techniques DNA Fingerprinting DNA, Bacterial - genetics Electrophoresis, Gel, Pulsed-Field Enterococcus - classification Enterococcus - drug effects Enterococcus - genetics Gram-Positive Bacterial Infections - microbiology Humans Polymerase Chain Reaction - methods Repetitive Sequences, Nucleic Acid - genetics Vancomycin Resistance |
| title | Clinical evaluation of repetitive sequence-based polymerase chain reaction using the Diversi-Lab System for strain typing of vancomycin-resistant enterococci |
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