Effect of carbon and nitrogen sources, pH and temperature on in vitro culture of several isolates of Amanita caesarea (Scop.:Fr.) Pers
Several isolates were obtained from sporocarps of Amanita caesarea (Scop.: Fr.) Pers. associated with Quercus suber and Castanea sativa coming from the southwest of Spain. Culture conditions were optimized for these isolates. The largest radial growth was obtained at pH 6-7, and optimal growth tempe...
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| Veröffentlicht in: | Mycorrhiza 2006-03, Vol.16 (2), p.133-136 |
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| creator | DAZA, A MANJON, J. L CAMACHO, M ROMERO DE LA OSA, L AGUILAR, A SANTAMARIA, C |
| description | Several isolates were obtained from sporocarps of Amanita caesarea (Scop.: Fr.) Pers. associated with Quercus suber and Castanea sativa coming from the southwest of Spain. Culture conditions were optimized for these isolates. The largest radial growth was obtained at pH 6-7, and optimal growth temperature was 24-28 degrees C depending on the isolate. Albumin bovine and nitrate produced the largest patch size diameters, but the greatest mycelium dry weight yields were obtained with ammonium. Mannitol produced the largest radial growth, and mannitol and glucose yielded the biggest mycelium dry weights. Although variations in growth behaviour between isolates were observed, only one internal spacer sequence-restriction fragment length polymorphism type was obtained. |
| doi_str_mv | 10.1007/s00572-005-0025-6 |
| format | Article |
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Mannitol produced the largest radial growth, and mannitol and glucose yielded the biggest mycelium dry weights. Although variations in growth behaviour between isolates were observed, only one internal spacer sequence-restriction fragment length polymorphism type was obtained.</description><subject>Amanita</subject><subject>Amanita - genetics</subject><subject>Amanita - growth & development</subject><subject>Ammonium</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Carbon</subject><subject>Castanea sativa</subject><subject>Culture Media</subject><subject>Fagaceae - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Microbiology</subject><subject>Nitrogen</subject><subject>Nitrogen sources</subject><subject>Parasitism and symbiosis</subject><subject>Plant physiology and development</subject><subject>Polymorphism, Genetic</subject><subject>Quercus suber</subject><subject>Spain</subject><subject>Symbiosis</subject><subject>Temperature</subject><issn>0940-6360</issn><issn>1432-1890</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkdFqFTEQhoNY7Gn1AbyRICgK7nGyySZZ70ppbaGgoF6HJDuRLbubNdkt9AX63Ob0HCh440Um8Oebf5j8hLxmsGUA6nMGaFRdlVpO3VTyGdkwweuK6Raekw20AirJJRyTk5xvAZiSnL0gx0zWbd0o2JCHixDQLzQG6m1ycaJ26ujULyn-xonmuCaP-ROdrx4fFhxnTHZZE9LC9hO926HUr8NeCzTjXSEG2uc42AXzTjsbbbG0ZQRmm9DSDz98nLdfLtP2I_2OKb8kR8EOGV8d7lPy6_Li5_lVdfPt6_X52U3lRcOXSnGNrQuBAbKa-9Chk4CgHZdOeMeCQ6ECc44JBW1QouuctkK2ilvFAfkpeb_3nVP8s2JezNhnj8NgJ4xrNlIpxqCB_4JMgVYCRAHf_gPelj-byhJGsrrVquW6QGwP-RRzThjMnPrRpnvDwOyiNPsoTalmF6WRpefNwXh1I3ZPHYfsCvDuANjs7RCSnXyfnzjVaAma878evaZO</recordid><startdate>20060301</startdate><enddate>20060301</enddate><creator>DAZA, A</creator><creator>MANJON, J. 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| ispartof | Mycorrhiza, 2006-03, Vol.16 (2), p.133-136 |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Amanita Amanita - genetics Amanita - growth & development Ammonium Bacteria Biological and medical sciences Carbon Castanea sativa Culture Media Fagaceae - microbiology Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Microbiology Nitrogen Nitrogen sources Parasitism and symbiosis Plant physiology and development Polymorphism, Genetic Quercus suber Spain Symbiosis Temperature |
| title | Effect of carbon and nitrogen sources, pH and temperature on in vitro culture of several isolates of Amanita caesarea (Scop.:Fr.) Pers |
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