Improved superfusion technique for rapid cooling or heating of cultured cells under patch-clamp conditions

We have developed an improved technique for fast cooling and heating of solutions superfusing isolated cells under patch-clamp or calcium imaging conditions. The system meets the requirements for studying temperature dependency of all kinds of ion channels, in particular temperature-gated ion channe...

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Veröffentlicht in:	Journal of neuroscience methods 2006-03, Vol.151 (2), p.178-185
Hauptverfasser:	Dittert, Ivan, Benedikt, Jan, Vyklický, Ladislav, Zimmermann, Katharina, Reeh, Peter W., Vlachová, Viktorie
Format:	Artikel
Sprache:	eng
Schlagworte:	Action Potentials - physiology Animals Capsaicin Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Cells, Cultured Cold Temperature Environment, Controlled Equipment Design Equipment Failure Analysis Feedback controlled Peltier device Hot Temperature Humans Membrane Potentials - physiology Menthol Neurons, Afferent - physiology Patch-Clamp Techniques - instrumentation Patch-Clamp Techniques - methods Perfusion - instrumentation Rats Temperature control TRPM8 TRPV1
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