Effects of Crotalus durissus collilineatus venom in the isolated rat kidney

Ophidian accidents caused by the subspecies Crotalus durissus are responsible for high morbity and mortality rates. Acute renal failure is a common complication observed in these accidents. The aim of the present study was to investigate the renal effects promoted by the venom of C. d. collilineatus...

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Veröffentlicht in:Toxicon (Oxford) 2006-03, Vol.47 (3), p.260-264
Hauptverfasser: Amora, Daniela N., Sousa, Ticiana M., Martins, Alice M.C., Barbosa, Paulo S.F., Magalhães, Marta R., Toyama, Marcus H., Fonteles, Manassés C., Menezes, Dalgimar B. de, Monteiro, Helena S.A.
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container_issue 3
container_start_page 260
container_title Toxicon (Oxford)
container_volume 47
creator Amora, Daniela N.
Sousa, Ticiana M.
Martins, Alice M.C.
Barbosa, Paulo S.F.
Magalhães, Marta R.
Toyama, Marcus H.
Fonteles, Manassés C.
Menezes, Dalgimar B. de
Monteiro, Helena S.A.
description Ophidian accidents caused by the subspecies Crotalus durissus are responsible for high morbity and mortality rates. Acute renal failure is a common complication observed in these accidents. The aim of the present study was to investigate the renal effects promoted by the venom of C. d. collilineatus and its fractions, crotoxin and phospholipase A 2. C. d. collilineatus (Cdc; 30 μg mL −1), crotoxin (CTX; 10 μg mL −1) and phospholipase A 2 (PLA 2; 10 μg mL −1) were tested in isolated rat kidney. The first 30 min of each experiment were used as an internal control and Cdc or its fractions, CTX and PLA 2 were added to the system after this period. All experiments lasted 120 min. The venom of Cdc decreased perfusion pressure (PP; control 120=110.3±3.69 mmHg; Cdc 120=96.7±8.1 mmHg), renal vascular resistance (RVR; control 120=6.42±0.78 mmHg mL g −1 min −1; Cdc 120=4.8±0.56 mmHg/mL g −1 min −1), urinary flow (UF; control 120=0.19±0.03 mL g −1 min −1; Cdc 120=0.12±0.01 mL g −1 min −1), and glomerular filtration rate (GFR; control 120=0.79±0.07 mL g −1 min −1; Cdc 120=0.53±0.09 mL g −1 min −1), but had no effect on the percent of sodium tubular transport (%TNa +), percent of chloride tubular transport (%TK +) and percent of potassium tubular transport (%TCl −). CTX and PLA 2 reduced the GFR, while UF, PP and RVR remained stable during the full 120 min of perfusion. Crotoxin administration also diminished the %TK + (control 120=69.94±6.49; CTX 120=33.28±4.78) and %TCl − (control 120=79.53±2.67; CTX 120=64.62±6.93). PLA 2 reduced the %TK +, but exerted no effect on the %TNa + or on that of TCl −. In conclusion, the C. d. collilineatus venom altered the renal functional parameters evaluated. We suggest that crotoxin and phospholipase A 2 were involved in this process, since the renal effects observed would be due to the synergistic action of the components of the venom.
doi_str_mv 10.1016/j.toxicon.2005.10.007
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Acute renal failure is a common complication observed in these accidents. The aim of the present study was to investigate the renal effects promoted by the venom of C. d. collilineatus and its fractions, crotoxin and phospholipase A 2. C. d. collilineatus (Cdc; 30 μg mL −1), crotoxin (CTX; 10 μg mL −1) and phospholipase A 2 (PLA 2; 10 μg mL −1) were tested in isolated rat kidney. The first 30 min of each experiment were used as an internal control and Cdc or its fractions, CTX and PLA 2 were added to the system after this period. All experiments lasted 120 min. The venom of Cdc decreased perfusion pressure (PP; control 120=110.3±3.69 mmHg; Cdc 120=96.7±8.1 mmHg), renal vascular resistance (RVR; control 120=6.42±0.78 mmHg mL g −1 min −1; Cdc 120=4.8±0.56 mmHg/mL g −1 min −1), urinary flow (UF; control 120=0.19±0.03 mL g −1 min −1; Cdc 120=0.12±0.01 mL g −1 min −1), and glomerular filtration rate (GFR; control 120=0.79±0.07 mL g −1 min −1; Cdc 120=0.53±0.09 mL g −1 min −1), but had no effect on the percent of sodium tubular transport (%TNa +), percent of chloride tubular transport (%TK +) and percent of potassium tubular transport (%TCl −). CTX and PLA 2 reduced the GFR, while UF, PP and RVR remained stable during the full 120 min of perfusion. Crotoxin administration also diminished the %TK + (control 120=69.94±6.49; CTX 120=33.28±4.78) and %TCl − (control 120=79.53±2.67; CTX 120=64.62±6.93). PLA 2 reduced the %TK +, but exerted no effect on the %TNa + or on that of TCl −. 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Acute renal failure is a common complication observed in these accidents. The aim of the present study was to investigate the renal effects promoted by the venom of C. d. collilineatus and its fractions, crotoxin and phospholipase A 2. C. d. collilineatus (Cdc; 30 μg mL −1), crotoxin (CTX; 10 μg mL −1) and phospholipase A 2 (PLA 2; 10 μg mL −1) were tested in isolated rat kidney. The first 30 min of each experiment were used as an internal control and Cdc or its fractions, CTX and PLA 2 were added to the system after this period. All experiments lasted 120 min. The venom of Cdc decreased perfusion pressure (PP; control 120=110.3±3.69 mmHg; Cdc 120=96.7±8.1 mmHg), renal vascular resistance (RVR; control 120=6.42±0.78 mmHg mL g −1 min −1; Cdc 120=4.8±0.56 mmHg/mL g −1 min −1), urinary flow (UF; control 120=0.19±0.03 mL g −1 min −1; Cdc 120=0.12±0.01 mL g −1 min −1), and glomerular filtration rate (GFR; control 120=0.79±0.07 mL g −1 min −1; Cdc 120=0.53±0.09 mL g −1 min −1), but had no effect on the percent of sodium tubular transport (%TNa +), percent of chloride tubular transport (%TK +) and percent of potassium tubular transport (%TCl −). CTX and PLA 2 reduced the GFR, while UF, PP and RVR remained stable during the full 120 min of perfusion. Crotoxin administration also diminished the %TK + (control 120=69.94±6.49; CTX 120=33.28±4.78) and %TCl − (control 120=79.53±2.67; CTX 120=64.62±6.93). PLA 2 reduced the %TK +, but exerted no effect on the %TNa + or on that of TCl −. 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Acute renal failure is a common complication observed in these accidents. The aim of the present study was to investigate the renal effects promoted by the venom of C. d. collilineatus and its fractions, crotoxin and phospholipase A 2. C. d. collilineatus (Cdc; 30 μg mL −1), crotoxin (CTX; 10 μg mL −1) and phospholipase A 2 (PLA 2; 10 μg mL −1) were tested in isolated rat kidney. The first 30 min of each experiment were used as an internal control and Cdc or its fractions, CTX and PLA 2 were added to the system after this period. All experiments lasted 120 min. The venom of Cdc decreased perfusion pressure (PP; control 120=110.3±3.69 mmHg; Cdc 120=96.7±8.1 mmHg), renal vascular resistance (RVR; control 120=6.42±0.78 mmHg mL g −1 min −1; Cdc 120=4.8±0.56 mmHg/mL g −1 min −1), urinary flow (UF; control 120=0.19±0.03 mL g −1 min −1; Cdc 120=0.12±0.01 mL g −1 min −1), and glomerular filtration rate (GFR; control 120=0.79±0.07 mL g −1 min −1; Cdc 120=0.53±0.09 mL g −1 min −1), but had no effect on the percent of sodium tubular transport (%TNa +), percent of chloride tubular transport (%TK +) and percent of potassium tubular transport (%TCl −). CTX and PLA 2 reduced the GFR, while UF, PP and RVR remained stable during the full 120 min of perfusion. Crotoxin administration also diminished the %TK + (control 120=69.94±6.49; CTX 120=33.28±4.78) and %TCl − (control 120=79.53±2.67; CTX 120=64.62±6.93). PLA 2 reduced the %TK +, but exerted no effect on the %TNa + or on that of TCl −. In conclusion, the C. d. collilineatus venom altered the renal functional parameters evaluated. We suggest that crotoxin and phospholipase A 2 were involved in this process, since the renal effects observed would be due to the synergistic action of the components of the venom.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>16427672</pmid><doi>10.1016/j.toxicon.2005.10.007</doi><tpages>5</tpages></addata></record>
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subjects Animal poisons toxicology. Antivenoms
Animals
Biological and medical sciences
C.d. collilineatus
Crotalid Venoms - administration & dosage
Crotalid Venoms - pharmacology
Crotalus
Crotalus durissus
Crotalus durissus collilineatus
Crotoxin - administration & dosage
Crotoxin - pharmacology
Female
Glomerular Filtration Rate - drug effects
kidney
Kidney - drug effects
Kidney - physiology
Male
Medical sciences
Phospholipases A - administration & dosage
Phospholipases A - pharmacology
Phospholipases A2
Rats
Rats, Wistar
Toxicology
Vascular Resistance - drug effects
venom
title Effects of Crotalus durissus collilineatus venom in the isolated rat kidney
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