Use of housekeeping gene sequencing for species identification of viridans streptococci
Based on the genetic analysis of 20 reference strains, we describe an approach using the sequencing of 2 housekeeping genes, zwf and gki, to identify members of the mitis–sanguinis group of viridans streptococci to the species level, with a better discrimination compared with 16S rDNA sequencing. Th...
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Veröffentlicht in: | Diagnostic microbiology and infectious disease 2005-04, Vol.51 (4), p.297-301 |
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creator | Kiratisin, Pattarachai Li, Li Murray, Patrick R. Fischer, Steven H. |
description | Based on the genetic analysis of 20 reference strains, we describe an approach using the sequencing of 2 housekeeping genes,
zwf and
gki, to identify members of the
mitis–sanguinis group of viridans streptococci to the species level, with a better discrimination compared with 16S rDNA sequencing. This approach also suggested that some reference strains may not be correctly classified. |
doi_str_mv | 10.1016/j.diagmicrobio.2004.12.001 |
format | Article |
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gki, to identify members of the
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zwf and
gki, to identify members of the
mitis–sanguinis group of viridans streptococci to the species level, with a better discrimination compared with 16S rDNA sequencing. This approach also suggested that some reference strains may not be correctly classified.</description><subject>Bacterial Typing Techniques</subject><subject>Biological and medical sciences</subject><subject>DNA, Bacterial - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gki</subject><subject>Glucokinase - genetics</subject><subject>Glucosephosphate Dehydrogenase - genetics</subject><subject>Housekeeping genes</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Sequence Analysis, DNA</subject><subject>Species Specificity</subject><subject>Streptococcus</subject><subject>Viridans Streptococci - classification</subject><subject>Viridans Streptococci - genetics</subject><subject>zwf</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtvEzEQgC0EomnhL6AVEr3tMn7E9vaGWtoiVeJCxdHy2uPgkKy39qYS_x6HRGpv9DQz8jcPf4R8pNBRoPLzuvPRrrbR5TTE1DEA0VHWAdBXZEG16lsABa_JAhRnrdY9PyGnpawrwHoBb8kJXWrQnLEF-XlfsEmh-ZV2BX8jTnFcNSscsSn4sMPR7euQclMmdBFLEz2OcwzR2Tmmcd_6GHP0dixNmTNOc3LJufiOvAl2U_D9MZ6R--uvPy5v27vvN98uv9y1TvB-bqnQAoUNug82yF4PWnu5ZFBfBQgnYKiJkM55rZS0nlPhBs4UE5zRQXF-Rs4Pc6ec6r1lNttYHG42dsT6JSOVAq6A_hekSi4Fl1DBiwNY9ZaSMZgpx63NfwwFs_dv1ua5f7P3bygz8G_Lh-OW3bBF_9R6FF6BT0fAFmc3IduquDxxUjEKQlXu6sBhlfcYMZtS9Y8OfczoZuNTfMk9fwH7O6p2</recordid><startdate>20050401</startdate><enddate>20050401</enddate><creator>Kiratisin, Pattarachai</creator><creator>Li, Li</creator><creator>Murray, Patrick R.</creator><creator>Fischer, Steven H.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20050401</creationdate><title>Use of housekeeping gene sequencing for species identification of viridans streptococci</title><author>Kiratisin, Pattarachai ; Li, Li ; Murray, Patrick R. ; Fischer, Steven H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-1484e4af89faf698b88d6520c43404c40b43446ccd8776ad314cb32724321b733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Bacterial Typing Techniques</topic><topic>Biological and medical sciences</topic><topic>DNA, Bacterial - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gki</topic><topic>Glucokinase - genetics</topic><topic>Glucosephosphate Dehydrogenase - genetics</topic><topic>Housekeeping genes</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Sequence Analysis, DNA</topic><topic>Species Specificity</topic><topic>Streptococcus</topic><topic>Viridans Streptococci - classification</topic><topic>Viridans Streptococci - genetics</topic><topic>zwf</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kiratisin, Pattarachai</creatorcontrib><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Murray, Patrick R.</creatorcontrib><creatorcontrib>Fischer, Steven H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kiratisin, Pattarachai</au><au>Li, Li</au><au>Murray, Patrick R.</au><au>Fischer, Steven H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of housekeeping gene sequencing for species identification of viridans streptococci</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>2005-04-01</date><risdate>2005</risdate><volume>51</volume><issue>4</issue><spage>297</spage><epage>301</epage><pages>297-301</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><coden>DMIDDZ</coden><abstract>Based on the genetic analysis of 20 reference strains, we describe an approach using the sequencing of 2 housekeeping genes,
zwf and
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subjects | Bacterial Typing Techniques Biological and medical sciences DNA, Bacterial - analysis Fundamental and applied biological sciences. Psychology gki Glucokinase - genetics Glucosephosphate Dehydrogenase - genetics Housekeeping genes Humans Infectious diseases Medical sciences Microbiology Sequence Analysis, DNA Species Specificity Streptococcus Viridans Streptococci - classification Viridans Streptococci - genetics zwf |
title | Use of housekeeping gene sequencing for species identification of viridans streptococci |
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