Use of housekeeping gene sequencing for species identification of viridans streptococci

Based on the genetic analysis of 20 reference strains, we describe an approach using the sequencing of 2 housekeeping genes, zwf and gki, to identify members of the mitis–sanguinis group of viridans streptococci to the species level, with a better discrimination compared with 16S rDNA sequencing. Th...

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Veröffentlicht in:Diagnostic microbiology and infectious disease 2005-04, Vol.51 (4), p.297-301
Hauptverfasser: Kiratisin, Pattarachai, Li, Li, Murray, Patrick R., Fischer, Steven H.
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Li, Li
Murray, Patrick R.
Fischer, Steven H.
description Based on the genetic analysis of 20 reference strains, we describe an approach using the sequencing of 2 housekeeping genes, zwf and gki, to identify members of the mitis–sanguinis group of viridans streptococci to the species level, with a better discrimination compared with 16S rDNA sequencing. This approach also suggested that some reference strains may not be correctly classified.
doi_str_mv 10.1016/j.diagmicrobio.2004.12.001
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subjects Bacterial Typing Techniques
Biological and medical sciences
DNA, Bacterial - analysis
Fundamental and applied biological sciences. Psychology
gki
Glucokinase - genetics
Glucosephosphate Dehydrogenase - genetics
Housekeeping genes
Humans
Infectious diseases
Medical sciences
Microbiology
Sequence Analysis, DNA
Species Specificity
Streptococcus
Viridans Streptococci - classification
Viridans Streptococci - genetics
zwf
title Use of housekeeping gene sequencing for species identification of viridans streptococci
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