Characterization of a novel repetitive secretory protein specifically expressed in the modified salivary gland of Hydropsyche sp. (Trichoptera; Hydropsychidae)
Here, we report the cloning and characterization of a common salivary gland-specific gene, Nf-1, from late instar Hydropsyche sp. larvae, and show that the corresponding gene product is translated in the gland and secreted to the gland lumen. The deduced Nf-1 protein is primarily composed of five re...
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| Veröffentlicht in: | Insect biochemistry and molecular biology 2005-05, Vol.35 (5), p.435-441 |
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| container_title | Insect biochemistry and molecular biology |
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| creator | Eum, Jai-Hoon Yoe, Sung-Moon Seo, Young-R Kang, Seok-Woo Han, Sung-Sik |
| description | Here, we report the cloning and characterization of a common salivary gland-specific gene,
Nf-1, from late instar
Hydropsyche sp. larvae, and show that the corresponding gene product is translated in the gland and secreted to the gland lumen. The deduced Nf-1 protein is primarily composed of five repetitive sequence units of 63–65 amino acids, and contains a putative signal sequence composed of 19 amino acids. Secreted Nf-1 (∼37
kDa) was localized to the gland lumen by Western blotting of gland and lumen fractions. Together, the structure, expression pattern and protein localization of Nf-1 indicate that this protein is likely to be a major component of the silk shields and nets produced by the aquatic insect, Trichoptera. |
| doi_str_mv | 10.1016/j.ibmb.2005.01.009 |
| format | Article |
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Nf-1, from late instar
Hydropsyche sp. larvae, and show that the corresponding gene product is translated in the gland and secreted to the gland lumen. The deduced Nf-1 protein is primarily composed of five repetitive sequence units of 63–65 amino acids, and contains a putative signal sequence composed of 19 amino acids. Secreted Nf-1 (∼37
kDa) was localized to the gland lumen by Western blotting of gland and lumen fractions. Together, the structure, expression pattern and protein localization of Nf-1 indicate that this protein is likely to be a major component of the silk shields and nets produced by the aquatic insect, Trichoptera.</description><identifier>ISSN: 0965-1748</identifier><identifier>EISSN: 1879-0240</identifier><identifier>DOI: 10.1016/j.ibmb.2005.01.009</identifier><identifier>PMID: 15804577</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>amino acid sequences ; Animals ; aquatic insects ; Base Sequence ; complementary DNA ; Expressed sequence tags ; gene expression ; Hydropsyche ; Hydropsychidae ; insect proteins ; Insect Proteins - biosynthesis ; Insect Proteins - genetics ; Insecta - genetics ; Insecta - metabolism ; messenger RNA ; Modified salivary gland ; Molecular Sequence Data ; nest forming protein-1 ; Nf-1 protein (Nest forming protein-1) ; nucleotide sequences ; protein secretion ; Repetitive amino acid ; repetitive sequences ; salivary glands ; Salivary Glands - metabolism ; silk ; silk glands ; Trichoptera ; Trichoptera Hydropsyche sp</subject><ispartof>Insect biochemistry and molecular biology, 2005-05, Vol.35 (5), p.435-441</ispartof><rights>2005 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-ec05973d1b2b06437135a68caa2c20868a5baaaa83b860ad972ee481c44bbb213</citedby><cites>FETCH-LOGICAL-c440t-ec05973d1b2b06437135a68caa2c20868a5baaaa83b860ad972ee481c44bbb213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0965174805000238$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15804577$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eum, Jai-Hoon</creatorcontrib><creatorcontrib>Yoe, Sung-Moon</creatorcontrib><creatorcontrib>Seo, Young-R</creatorcontrib><creatorcontrib>Kang, Seok-Woo</creatorcontrib><creatorcontrib>Han, Sung-Sik</creatorcontrib><title>Characterization of a novel repetitive secretory protein specifically expressed in the modified salivary gland of Hydropsyche sp. (Trichoptera; Hydropsychidae)</title><title>Insect biochemistry and molecular biology</title><addtitle>Insect Biochem Mol Biol</addtitle><description>Here, we report the cloning and characterization of a common salivary gland-specific gene,
Nf-1, from late instar
Hydropsyche sp. larvae, and show that the corresponding gene product is translated in the gland and secreted to the gland lumen. The deduced Nf-1 protein is primarily composed of five repetitive sequence units of 63–65 amino acids, and contains a putative signal sequence composed of 19 amino acids. Secreted Nf-1 (∼37
kDa) was localized to the gland lumen by Western blotting of gland and lumen fractions. Together, the structure, expression pattern and protein localization of Nf-1 indicate that this protein is likely to be a major component of the silk shields and nets produced by the aquatic insect, Trichoptera.</description><subject>amino acid sequences</subject><subject>Animals</subject><subject>aquatic insects</subject><subject>Base Sequence</subject><subject>complementary DNA</subject><subject>Expressed sequence tags</subject><subject>gene expression</subject><subject>Hydropsyche</subject><subject>Hydropsychidae</subject><subject>insect proteins</subject><subject>Insect Proteins - biosynthesis</subject><subject>Insect Proteins - genetics</subject><subject>Insecta - genetics</subject><subject>Insecta - metabolism</subject><subject>messenger RNA</subject><subject>Modified salivary gland</subject><subject>Molecular Sequence Data</subject><subject>nest forming protein-1</subject><subject>Nf-1 protein (Nest forming protein-1)</subject><subject>nucleotide sequences</subject><subject>protein secretion</subject><subject>Repetitive amino acid</subject><subject>repetitive sequences</subject><subject>salivary glands</subject><subject>Salivary Glands - metabolism</subject><subject>silk</subject><subject>silk glands</subject><subject>Trichoptera</subject><subject>Trichoptera Hydropsyche sp</subject><issn>0965-1748</issn><issn>1879-0240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2O0zAUhSMEYsrAC7AArxAsEq6dHzsaNqgCBmkkFsysLce5mbpK4mC7FeVleFVu1UqwYryxrfudo2OfLHvJoeDAm_fbwnVTVwiAugBeALSPshVXss1BVPA4W0Hb1DmXlbrInsW4BYCqquXT7ILXCugkV9nv9cYEYxMG98sk52fmB2bY7Pc4soALJpfcHllEGzD5cGBL8AndzOKC1g3OmnE8MPy5BIwRe0aTtEE2-Z6GdI9mdHtDuvvRzP3R_frQB7_EgyUsLgV7exuc3fiFMpirf6auN_juefZkMGPEF-f9Mrv7_Ol2fZ3ffPvydf3xJrdVBSlHC3Ury553ooOmKiUva9Moa4ywAlSjTN0ZWqrsVAOmb6VArBQnddd1gpeX2ZuTLz3vxw5j0pOLFkcKjX4XdSMlCEHOD4ECpKQo9YMglzUowYFAcQJt8DEGHPQS3ERfpjnoY9F6q49F62PRGrimokn06uy-6ybs_0rOzRLw-gQMxmtzH1zUd98F8JLUSolaEfHhRCD9695h0NE6nC32LqBNuvfufwn-ANfvxlQ</recordid><startdate>20050501</startdate><enddate>20050501</enddate><creator>Eum, Jai-Hoon</creator><creator>Yoe, Sung-Moon</creator><creator>Seo, Young-R</creator><creator>Kang, Seok-Woo</creator><creator>Han, Sung-Sik</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050501</creationdate><title>Characterization of a novel repetitive secretory protein specifically expressed in the modified salivary gland of Hydropsyche sp. (Trichoptera; Hydropsychidae)</title><author>Eum, Jai-Hoon ; Yoe, Sung-Moon ; Seo, Young-R ; Kang, Seok-Woo ; Han, Sung-Sik</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-ec05973d1b2b06437135a68caa2c20868a5baaaa83b860ad972ee481c44bbb213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>amino acid sequences</topic><topic>Animals</topic><topic>aquatic insects</topic><topic>Base Sequence</topic><topic>complementary DNA</topic><topic>Expressed sequence tags</topic><topic>gene expression</topic><topic>Hydropsyche</topic><topic>Hydropsychidae</topic><topic>insect proteins</topic><topic>Insect Proteins - biosynthesis</topic><topic>Insect Proteins - genetics</topic><topic>Insecta - genetics</topic><topic>Insecta - metabolism</topic><topic>messenger RNA</topic><topic>Modified salivary gland</topic><topic>Molecular Sequence Data</topic><topic>nest forming protein-1</topic><topic>Nf-1 protein (Nest forming protein-1)</topic><topic>nucleotide sequences</topic><topic>protein secretion</topic><topic>Repetitive amino acid</topic><topic>repetitive sequences</topic><topic>salivary glands</topic><topic>Salivary Glands - metabolism</topic><topic>silk</topic><topic>silk glands</topic><topic>Trichoptera</topic><topic>Trichoptera Hydropsyche sp</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eum, Jai-Hoon</creatorcontrib><creatorcontrib>Yoe, Sung-Moon</creatorcontrib><creatorcontrib>Seo, Young-R</creatorcontrib><creatorcontrib>Kang, Seok-Woo</creatorcontrib><creatorcontrib>Han, Sung-Sik</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Insect biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eum, Jai-Hoon</au><au>Yoe, Sung-Moon</au><au>Seo, Young-R</au><au>Kang, Seok-Woo</au><au>Han, Sung-Sik</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a novel repetitive secretory protein specifically expressed in the modified salivary gland of Hydropsyche sp. (Trichoptera; Hydropsychidae)</atitle><jtitle>Insect biochemistry and molecular biology</jtitle><addtitle>Insect Biochem Mol Biol</addtitle><date>2005-05-01</date><risdate>2005</risdate><volume>35</volume><issue>5</issue><spage>435</spage><epage>441</epage><pages>435-441</pages><issn>0965-1748</issn><eissn>1879-0240</eissn><abstract>Here, we report the cloning and characterization of a common salivary gland-specific gene,
Nf-1, from late instar
Hydropsyche sp. larvae, and show that the corresponding gene product is translated in the gland and secreted to the gland lumen. The deduced Nf-1 protein is primarily composed of five repetitive sequence units of 63–65 amino acids, and contains a putative signal sequence composed of 19 amino acids. Secreted Nf-1 (∼37
kDa) was localized to the gland lumen by Western blotting of gland and lumen fractions. Together, the structure, expression pattern and protein localization of Nf-1 indicate that this protein is likely to be a major component of the silk shields and nets produced by the aquatic insect, Trichoptera.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>15804577</pmid><doi>10.1016/j.ibmb.2005.01.009</doi><tpages>7</tpages></addata></record> |
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| subjects | amino acid sequences Animals aquatic insects Base Sequence complementary DNA Expressed sequence tags gene expression Hydropsyche Hydropsychidae insect proteins Insect Proteins - biosynthesis Insect Proteins - genetics Insecta - genetics Insecta - metabolism messenger RNA Modified salivary gland Molecular Sequence Data nest forming protein-1 Nf-1 protein (Nest forming protein-1) nucleotide sequences protein secretion Repetitive amino acid repetitive sequences salivary glands Salivary Glands - metabolism silk silk glands Trichoptera Trichoptera Hydropsyche sp |
| title | Characterization of a novel repetitive secretory protein specifically expressed in the modified salivary gland of Hydropsyche sp. (Trichoptera; Hydropsychidae) |
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