Functional Specificity of the Xenopus T-Domain Protein Brachyury Is Conferred by Its Ability to Interact with Smad1
Members of the T-box gene family play important and diverse roles in development and disease. Here, we study the functional specificities of the Xenopus T-domain proteins Xbra and VegT, which differ in their abilities to induce gene expression in prospective ectodermal tissue. In particular, VegT in...
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| Veröffentlicht in: | Developmental cell 2005-04, Vol.8 (4), p.599-610 |
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| creator | Messenger, Nigel J. Kabitschke, Christin Andrews, Robert Grimmer, Donna Miguel, Ricardo Núñez Blundell, Tom L. Smith, James C. Wardle, Fiona C. |
| description | Members of the T-box gene family play important and diverse roles in development and disease. Here, we study the functional specificities of the
Xenopus T-domain proteins Xbra and VegT, which differ in their abilities to induce gene expression in prospective ectodermal tissue. In particular, VegT induces strong expression of
goosecoid whereas Xbra cannot. Our results indicate that Xbra is unable to induce
goosecoid because it directly activates expression of
Xom, a repressor of
goosecoid that acts downstream of BMP signaling. We show that the inability of Xbra to induce
goosecoid is imposed by an N-terminal domain that interacts with the C-terminal MH2 domain of Smad1, a component of the BMP signal transduction pathway. Interference with this interaction causes ectopic activation of
goosecoid and anteriorization of the embryo. These findings suggest a mechanism by which individual T-domain proteins may interact with different partners to elicit a specific response. |
| doi_str_mv | 10.1016/j.devcel.2005.03.001 |
| format | Article |
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Xenopus T-domain proteins Xbra and VegT, which differ in their abilities to induce gene expression in prospective ectodermal tissue. In particular, VegT induces strong expression of
goosecoid whereas Xbra cannot. Our results indicate that Xbra is unable to induce
goosecoid because it directly activates expression of
Xom, a repressor of
goosecoid that acts downstream of BMP signaling. We show that the inability of Xbra to induce
goosecoid is imposed by an N-terminal domain that interacts with the C-terminal MH2 domain of Smad1, a component of the BMP signal transduction pathway. Interference with this interaction causes ectopic activation of
goosecoid and anteriorization of the embryo. These findings suggest a mechanism by which individual T-domain proteins may interact with different partners to elicit a specific response.</description><identifier>ISSN: 1534-5807</identifier><identifier>EISSN: 1878-1551</identifier><identifier>DOI: 10.1016/j.devcel.2005.03.001</identifier><identifier>PMID: 15809041</identifier><language>eng</language><publisher>Cambridge, MA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Biological and medical sciences ; Bone Morphogenetic Proteins - metabolism ; Cell differentiation, maturation, development, hematopoiesis ; Cell physiology ; DNA-Binding Proteins - chemistry ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Ectoderm - cytology ; Ectoderm - physiology ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental ; Goosecoid Protein ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Humans ; Models, Molecular ; Molecular and cellular biology ; Molecular Sequence Data ; Morphogenesis ; Protein Conformation ; Protein Structure, Tertiary ; Repressor Proteins - genetics ; Repressor Proteins - metabolism ; Sequence Alignment ; Signal Transduction - physiology ; Smad Proteins ; Smad1 Protein ; T-Box Domain Proteins - chemistry ; T-Box Domain Proteins - genetics ; T-Box Domain Proteins - metabolism ; Trans-Activators - chemistry ; Trans-Activators - genetics ; Trans-Activators - metabolism ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Two-Hybrid System Techniques ; Xenopus laevis - embryology ; Xenopus laevis - physiology ; Xenopus Proteins - genetics ; Xenopus Proteins - metabolism</subject><ispartof>Developmental cell, 2005-04, Vol.8 (4), p.599-610</ispartof><rights>2005 Elsevier Inc.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-9f0db7c4c3a4050b5ccf35b6caa44ee0b9243d2026804e3f3cde31d1131b41243</citedby><cites>FETCH-LOGICAL-c436t-9f0db7c4c3a4050b5ccf35b6caa44ee0b9243d2026804e3f3cde31d1131b41243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.devcel.2005.03.001$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16757765$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15809041$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Messenger, Nigel J.</creatorcontrib><creatorcontrib>Kabitschke, Christin</creatorcontrib><creatorcontrib>Andrews, Robert</creatorcontrib><creatorcontrib>Grimmer, Donna</creatorcontrib><creatorcontrib>Miguel, Ricardo Núñez</creatorcontrib><creatorcontrib>Blundell, Tom L.</creatorcontrib><creatorcontrib>Smith, James C.</creatorcontrib><creatorcontrib>Wardle, Fiona C.</creatorcontrib><title>Functional Specificity of the Xenopus T-Domain Protein Brachyury Is Conferred by Its Ability to Interact with Smad1</title><title>Developmental cell</title><addtitle>Dev Cell</addtitle><description>Members of the T-box gene family play important and diverse roles in development and disease. Here, we study the functional specificities of the
Xenopus T-domain proteins Xbra and VegT, which differ in their abilities to induce gene expression in prospective ectodermal tissue. In particular, VegT induces strong expression of
goosecoid whereas Xbra cannot. Our results indicate that Xbra is unable to induce
goosecoid because it directly activates expression of
Xom, a repressor of
goosecoid that acts downstream of BMP signaling. We show that the inability of Xbra to induce
goosecoid is imposed by an N-terminal domain that interacts with the C-terminal MH2 domain of Smad1, a component of the BMP signal transduction pathway. Interference with this interaction causes ectopic activation of
goosecoid and anteriorization of the embryo. These findings suggest a mechanism by which individual T-domain proteins may interact with different partners to elicit a specific response.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone Morphogenetic Proteins - metabolism</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell physiology</subject><subject>DNA-Binding Proteins - chemistry</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Ectoderm - cytology</subject><subject>Ectoderm - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Goosecoid Protein</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Humans</subject><subject>Models, Molecular</subject><subject>Molecular and cellular biology</subject><subject>Molecular Sequence Data</subject><subject>Morphogenesis</subject><subject>Protein Conformation</subject><subject>Protein Structure, Tertiary</subject><subject>Repressor Proteins - genetics</subject><subject>Repressor Proteins - metabolism</subject><subject>Sequence Alignment</subject><subject>Signal Transduction - physiology</subject><subject>Smad Proteins</subject><subject>Smad1 Protein</subject><subject>T-Box Domain Proteins - chemistry</subject><subject>T-Box Domain Proteins - genetics</subject><subject>T-Box Domain Proteins - metabolism</subject><subject>Trans-Activators - chemistry</subject><subject>Trans-Activators - genetics</subject><subject>Trans-Activators - metabolism</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Two-Hybrid System Techniques</subject><subject>Xenopus laevis - embryology</subject><subject>Xenopus laevis - physiology</subject><subject>Xenopus Proteins - genetics</subject><subject>Xenopus Proteins - metabolism</subject><issn>1534-5807</issn><issn>1878-1551</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1rGzEQhpfS0nw0_6AUXdrbbkeWtLu-FFI3aQ2BBJJCbkIrjbDMruRK2hT_-8rYkFtPMyM98zI8VfWRQkOBtl-3jcEXjWOzABANsAaAvqnOad_1NRWCvi29YLwWPXRn1UVK2wK0tIf31Rktj0vg9LxKt7PX2QWvRvK4Q-2s0y7vSbAkb5A8ow-7OZGn-keYlPPkIYaMpX6PSm_2c9yTdSKr4C3GiIYMZc6JXA9uPKTkQNY-Y2Ez-evyhjxOytAP1TurxoRXp3pZ_b69eVr9qu_uf65X13e15qzN9dKCGTrNNVMcBAxCa8vE0GqlOEeEYbngzCxg0fbAkVmmDTJqKGV04LT8XVZfjrm7GP7MmLKcXCrGRuUxzEm2XQcUGCsgP4I6hpQiWrmLblJxLynIg2y5lUfZ8iBbApPFZVn7dMqfhwnN69LJbgE-nwCVtBptVF679Mq1nei6VhTu25HDYuPFYZRJO_QajYuoszTB_f-Sf-konys</recordid><startdate>20050401</startdate><enddate>20050401</enddate><creator>Messenger, Nigel J.</creator><creator>Kabitschke, Christin</creator><creator>Andrews, Robert</creator><creator>Grimmer, Donna</creator><creator>Miguel, Ricardo Núñez</creator><creator>Blundell, Tom L.</creator><creator>Smith, James C.</creator><creator>Wardle, Fiona C.</creator><general>Elsevier Inc</general><general>Cell Press</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050401</creationdate><title>Functional Specificity of the Xenopus T-Domain Protein Brachyury Is Conferred by Its Ability to Interact with Smad1</title><author>Messenger, Nigel J. ; Kabitschke, Christin ; Andrews, Robert ; Grimmer, Donna ; Miguel, Ricardo Núñez ; Blundell, Tom L. ; Smith, James C. ; Wardle, Fiona C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-9f0db7c4c3a4050b5ccf35b6caa44ee0b9243d2026804e3f3cde31d1131b41243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bone Morphogenetic Proteins - metabolism</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell physiology</topic><topic>DNA-Binding Proteins - chemistry</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Ectoderm - cytology</topic><topic>Ectoderm - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Goosecoid Protein</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Humans</topic><topic>Models, Molecular</topic><topic>Molecular and cellular biology</topic><topic>Molecular Sequence Data</topic><topic>Morphogenesis</topic><topic>Protein Conformation</topic><topic>Protein Structure, Tertiary</topic><topic>Repressor Proteins - genetics</topic><topic>Repressor Proteins - metabolism</topic><topic>Sequence Alignment</topic><topic>Signal Transduction - physiology</topic><topic>Smad Proteins</topic><topic>Smad1 Protein</topic><topic>T-Box Domain Proteins - chemistry</topic><topic>T-Box Domain Proteins - genetics</topic><topic>T-Box Domain Proteins - metabolism</topic><topic>Trans-Activators - chemistry</topic><topic>Trans-Activators - genetics</topic><topic>Trans-Activators - metabolism</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Two-Hybrid System Techniques</topic><topic>Xenopus laevis - embryology</topic><topic>Xenopus laevis - physiology</topic><topic>Xenopus Proteins - genetics</topic><topic>Xenopus Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Messenger, Nigel J.</creatorcontrib><creatorcontrib>Kabitschke, Christin</creatorcontrib><creatorcontrib>Andrews, Robert</creatorcontrib><creatorcontrib>Grimmer, Donna</creatorcontrib><creatorcontrib>Miguel, Ricardo Núñez</creatorcontrib><creatorcontrib>Blundell, Tom L.</creatorcontrib><creatorcontrib>Smith, James C.</creatorcontrib><creatorcontrib>Wardle, Fiona C.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Messenger, Nigel J.</au><au>Kabitschke, Christin</au><au>Andrews, Robert</au><au>Grimmer, Donna</au><au>Miguel, Ricardo Núñez</au><au>Blundell, Tom L.</au><au>Smith, James C.</au><au>Wardle, Fiona C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional Specificity of the Xenopus T-Domain Protein Brachyury Is Conferred by Its Ability to Interact with Smad1</atitle><jtitle>Developmental cell</jtitle><addtitle>Dev Cell</addtitle><date>2005-04-01</date><risdate>2005</risdate><volume>8</volume><issue>4</issue><spage>599</spage><epage>610</epage><pages>599-610</pages><issn>1534-5807</issn><eissn>1878-1551</eissn><abstract>Members of the T-box gene family play important and diverse roles in development and disease. Here, we study the functional specificities of the
Xenopus T-domain proteins Xbra and VegT, which differ in their abilities to induce gene expression in prospective ectodermal tissue. In particular, VegT induces strong expression of
goosecoid whereas Xbra cannot. Our results indicate that Xbra is unable to induce
goosecoid because it directly activates expression of
Xom, a repressor of
goosecoid that acts downstream of BMP signaling. We show that the inability of Xbra to induce
goosecoid is imposed by an N-terminal domain that interacts with the C-terminal MH2 domain of Smad1, a component of the BMP signal transduction pathway. Interference with this interaction causes ectopic activation of
goosecoid and anteriorization of the embryo. These findings suggest a mechanism by which individual T-domain proteins may interact with different partners to elicit a specific response.</abstract><cop>Cambridge, MA</cop><pub>Elsevier Inc</pub><pmid>15809041</pmid><doi>10.1016/j.devcel.2005.03.001</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Developmental cell, 2005-04, Vol.8 (4), p.599-610 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Cell Press Free Archives; EZB Electronic Journals Library |
| subjects | Amino Acid Sequence Animals Biological and medical sciences Bone Morphogenetic Proteins - metabolism Cell differentiation, maturation, development, hematopoiesis Cell physiology DNA-Binding Proteins - chemistry DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Ectoderm - cytology Ectoderm - physiology Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental Goosecoid Protein Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Humans Models, Molecular Molecular and cellular biology Molecular Sequence Data Morphogenesis Protein Conformation Protein Structure, Tertiary Repressor Proteins - genetics Repressor Proteins - metabolism Sequence Alignment Signal Transduction - physiology Smad Proteins Smad1 Protein T-Box Domain Proteins - chemistry T-Box Domain Proteins - genetics T-Box Domain Proteins - metabolism Trans-Activators - chemistry Trans-Activators - genetics Trans-Activators - metabolism Transcription Factors - genetics Transcription Factors - metabolism Two-Hybrid System Techniques Xenopus laevis - embryology Xenopus laevis - physiology Xenopus Proteins - genetics Xenopus Proteins - metabolism |
| title | Functional Specificity of the Xenopus T-Domain Protein Brachyury Is Conferred by Its Ability to Interact with Smad1 |
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