Chicken embryo related (CER) cell line for quantification of rabies neutralizing antibody by fluorescent focus inhibition test
Levels of rabies virus neutralization antibody in sera from vaccinated dogs and cattle were either measured by mouse neutralization test (MNT) or by rapid fluorescent focus inhibition test (RFFIT), performed on CER monolayers. The two tests were compared for their ability to detect the 0.5 Internati...
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| Veröffentlicht in: | Biologicals 2006-03, Vol.34 (1), p.29-32 |
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| container_title | Biologicals |
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| creator | Cardoso, T.C. da Silva, L.H. Queiroz da Silva, S.E.L. Albas, A. Pardo, P.E. Tanaka, A.H. Cossy, L.B. Perri, S.H.V. |
| description | Levels of rabies virus neutralization antibody in sera from vaccinated dogs and cattle were either measured by mouse neutralization test (MNT) or by rapid fluorescent focus inhibition test (RFFIT), performed on CER monolayers. The two tests were compared for their ability to detect the 0.5 International Units/ml (I.U.) recommended by the World Health Organization (WHO) as the minimum response for proof of rabies immunization. A significant correlation was found between the two tests (
n
=
211;
r
=
0.9949 in dogs and 0.9307 in cows,
p
<
0.001), good sensitivity (87.5%), specificity (94.7%) and agreement (96.6%) as well. RFFIT method standardized on CER cell system for neutralizing antibodies detection turns the diagnosis easier and less expensive, specially when a great number of samples must be tested from endemic areas as commonly found in Brazil. |
| doi_str_mv | 10.1016/j.biologicals.2005.08.001 |
| format | Article |
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n
=
211;
r
=
0.9949 in dogs and 0.9307 in cows,
p
<
0.001), good sensitivity (87.5%), specificity (94.7%) and agreement (96.6%) as well. RFFIT method standardized on CER cell system for neutralizing antibodies detection turns the diagnosis easier and less expensive, specially when a great number of samples must be tested from endemic areas as commonly found in Brazil.</description><identifier>ISSN: 1045-1056</identifier><identifier>EISSN: 1095-8320</identifier><identifier>DOI: 10.1016/j.biologicals.2005.08.001</identifier><identifier>PMID: 16213749</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Antibodies, Viral - blood ; BHK-21 cells ; Cattle ; Cell Line ; CER (chicken embryo related) cells ; Chick Embryo - cytology ; Dogs ; Fluorescence ; Fluorescent Antibody Technique, Indirect - methods ; Mice ; Neutralization Tests ; Rabies ; Rabies - diagnosis ; Rabies virus ; Rabies virus - immunology ; Rapid focus fluorescent inhibition test (RFFIT) ; Sensitivity and Specificity</subject><ispartof>Biologicals, 2006-03, Vol.34 (1), p.29-32</ispartof><rights>2005 The International Association for Biologicals</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-354cddb7916d6d7fe095b3b39fd07fa150aa6cbbe417f6bdac9ef6a36274913</citedby><cites>FETCH-LOGICAL-c406t-354cddb7916d6d7fe095b3b39fd07fa150aa6cbbe417f6bdac9ef6a36274913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1045105605000771$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16213749$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cardoso, T.C.</creatorcontrib><creatorcontrib>da Silva, L.H. Queiroz</creatorcontrib><creatorcontrib>da Silva, S.E.L.</creatorcontrib><creatorcontrib>Albas, A.</creatorcontrib><creatorcontrib>Pardo, P.E.</creatorcontrib><creatorcontrib>Tanaka, A.H.</creatorcontrib><creatorcontrib>Cossy, L.B.</creatorcontrib><creatorcontrib>Perri, S.H.V.</creatorcontrib><title>Chicken embryo related (CER) cell line for quantification of rabies neutralizing antibody by fluorescent focus inhibition test</title><title>Biologicals</title><addtitle>Biologicals</addtitle><description>Levels of rabies virus neutralization antibody in sera from vaccinated dogs and cattle were either measured by mouse neutralization test (MNT) or by rapid fluorescent focus inhibition test (RFFIT), performed on CER monolayers. The two tests were compared for their ability to detect the 0.5 International Units/ml (I.U.) recommended by the World Health Organization (WHO) as the minimum response for proof of rabies immunization. A significant correlation was found between the two tests (
n
=
211;
r
=
0.9949 in dogs and 0.9307 in cows,
p
<
0.001), good sensitivity (87.5%), specificity (94.7%) and agreement (96.6%) as well. RFFIT method standardized on CER cell system for neutralizing antibodies detection turns the diagnosis easier and less expensive, specially when a great number of samples must be tested from endemic areas as commonly found in Brazil.</description><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>BHK-21 cells</subject><subject>Cattle</subject><subject>Cell Line</subject><subject>CER (chicken embryo related) cells</subject><subject>Chick Embryo - cytology</subject><subject>Dogs</subject><subject>Fluorescence</subject><subject>Fluorescent Antibody Technique, Indirect - methods</subject><subject>Mice</subject><subject>Neutralization Tests</subject><subject>Rabies</subject><subject>Rabies - diagnosis</subject><subject>Rabies virus</subject><subject>Rabies virus - immunology</subject><subject>Rapid focus fluorescent inhibition test (RFFIT)</subject><subject>Sensitivity and Specificity</subject><issn>1045-1056</issn><issn>1095-8320</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUuP1DAQhCMEYh_wF5C5IDgktMeJPTmiaGGRVkIC7pYf7V0PGXvXdpCGA78dhxkJbnDqPnzVpepqmpcUOgqUv9112sc53nqj5txtAIYOth0AfdScUxiHdss28Hjd-6GlMPCz5iLnXQVoL_qnzRnlG8pEP543P6c7b75hILjX6RBJwlkVtOT1dPX5DTE4z2T2AYmLiTwsKhTvqmvxMZDoSFLaYyYBl5LU7H_4cEtWRkd7IPpA3LzEhNlgKPWCWTLx4c5r_1tfMJdnzRNXM-Dz07xsvry_-jpdtzefPnyc3t20pgdeWjb0xlotRsott8JhDamZZqOzIJyiAyjFjdbYU-G4tsqM6LhifFNDUnbZvDpevU_xYam2cu_zmk0FjEuWXAgA4PBPkIr6Xsb6Co5H0KSYc0In75Pfq3SQFOTakdzJvzqSa0cStrJWULUvTiaL3qP9ozyVUoHpCGD9yHePSWbjMRi0PqEp0kb_Hza_AGgvq7Y</recordid><startdate>20060301</startdate><enddate>20060301</enddate><creator>Cardoso, T.C.</creator><creator>da Silva, L.H. Queiroz</creator><creator>da Silva, S.E.L.</creator><creator>Albas, A.</creator><creator>Pardo, P.E.</creator><creator>Tanaka, A.H.</creator><creator>Cossy, L.B.</creator><creator>Perri, S.H.V.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060301</creationdate><title>Chicken embryo related (CER) cell line for quantification of rabies neutralizing antibody by fluorescent focus inhibition test</title><author>Cardoso, T.C. ; da Silva, L.H. Queiroz ; da Silva, S.E.L. ; Albas, A. ; Pardo, P.E. ; Tanaka, A.H. ; Cossy, L.B. ; Perri, S.H.V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-354cddb7916d6d7fe095b3b39fd07fa150aa6cbbe417f6bdac9ef6a36274913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>BHK-21 cells</topic><topic>Cattle</topic><topic>Cell Line</topic><topic>CER (chicken embryo related) cells</topic><topic>Chick Embryo - cytology</topic><topic>Dogs</topic><topic>Fluorescence</topic><topic>Fluorescent Antibody Technique, Indirect - methods</topic><topic>Mice</topic><topic>Neutralization Tests</topic><topic>Rabies</topic><topic>Rabies - diagnosis</topic><topic>Rabies virus</topic><topic>Rabies virus - immunology</topic><topic>Rapid focus fluorescent inhibition test (RFFIT)</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cardoso, T.C.</creatorcontrib><creatorcontrib>da Silva, L.H. Queiroz</creatorcontrib><creatorcontrib>da Silva, S.E.L.</creatorcontrib><creatorcontrib>Albas, A.</creatorcontrib><creatorcontrib>Pardo, P.E.</creatorcontrib><creatorcontrib>Tanaka, A.H.</creatorcontrib><creatorcontrib>Cossy, L.B.</creatorcontrib><creatorcontrib>Perri, S.H.V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biologicals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cardoso, T.C.</au><au>da Silva, L.H. Queiroz</au><au>da Silva, S.E.L.</au><au>Albas, A.</au><au>Pardo, P.E.</au><au>Tanaka, A.H.</au><au>Cossy, L.B.</au><au>Perri, S.H.V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chicken embryo related (CER) cell line for quantification of rabies neutralizing antibody by fluorescent focus inhibition test</atitle><jtitle>Biologicals</jtitle><addtitle>Biologicals</addtitle><date>2006-03-01</date><risdate>2006</risdate><volume>34</volume><issue>1</issue><spage>29</spage><epage>32</epage><pages>29-32</pages><issn>1045-1056</issn><eissn>1095-8320</eissn><abstract>Levels of rabies virus neutralization antibody in sera from vaccinated dogs and cattle were either measured by mouse neutralization test (MNT) or by rapid fluorescent focus inhibition test (RFFIT), performed on CER monolayers. The two tests were compared for their ability to detect the 0.5 International Units/ml (I.U.) recommended by the World Health Organization (WHO) as the minimum response for proof of rabies immunization. A significant correlation was found between the two tests (
n
=
211;
r
=
0.9949 in dogs and 0.9307 in cows,
p
<
0.001), good sensitivity (87.5%), specificity (94.7%) and agreement (96.6%) as well. RFFIT method standardized on CER cell system for neutralizing antibodies detection turns the diagnosis easier and less expensive, specially when a great number of samples must be tested from endemic areas as commonly found in Brazil.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>16213749</pmid><doi>10.1016/j.biologicals.2005.08.001</doi><tpages>4</tpages></addata></record> |
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| ispartof | Biologicals, 2006-03, Vol.34 (1), p.29-32 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibodies, Viral - blood BHK-21 cells Cattle Cell Line CER (chicken embryo related) cells Chick Embryo - cytology Dogs Fluorescence Fluorescent Antibody Technique, Indirect - methods Mice Neutralization Tests Rabies Rabies - diagnosis Rabies virus Rabies virus - immunology Rapid focus fluorescent inhibition test (RFFIT) Sensitivity and Specificity |
| title | Chicken embryo related (CER) cell line for quantification of rabies neutralizing antibody by fluorescent focus inhibition test |
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