A subset of human rapidly self-renewing marrow stromal cells preferentially engraft in mice
Controversies have arisen as to whether adult stem cells or progenitor cells from bone marrow can engraft into nonhematopoietic tissues in vivo. To resolve some of the controversies, we developed a highly sensitive polymerase chain reaction-based single nucleotide polymorphism (PCR-SNP) assay for co...
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| Veröffentlicht in: | Blood 2006-03, Vol.107 (5), p.2153-2161 |
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| creator | Lee, Ryang Hwa Hsu, Shu Ching Munoz, James Jung, Jin Sup Lee, Na Rea Pochampally, Radhika Prockop, Darwin J. |
| description | Controversies have arisen as to whether adult stem cells or progenitor cells from bone marrow can engraft into nonhematopoietic tissues in vivo. To resolve some of the controversies, we developed a highly sensitive polymerase chain reaction-based single nucleotide polymorphism (PCR-SNP) assay for competitive engraftment of mixtures of stem/progenitor cells. We used the assay to follow engraftment in immunodeficient mice of subpopulations of the stem/progenitor cells from human bone marrow referred to as either mesenchymal stem cells or marrow stromal cells (MSCs). The engraftment into adult mice without induced tissue injury was low and variable, but there was preferential engraftment of a subpopulation of rapidly self-renewing MSCs (RS-MSCs) compared with a subpopulation of slowly renewing MSCs (SR-MSCs). After intravenous infusion, there was a tendency for the cells to engraft into the hippocampal region that was previously designated a “vascular niche.” Migration assays suggested that preferential engraftment of RS-MSCs was in part explained by their expression of CXCR4 and CX3R1, the receptors for SDF-1 and fractalkine. |
| doi_str_mv | 10.1182/blood-2005-07-2701 |
| format | Article |
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To resolve some of the controversies, we developed a highly sensitive polymerase chain reaction-based single nucleotide polymorphism (PCR-SNP) assay for competitive engraftment of mixtures of stem/progenitor cells. We used the assay to follow engraftment in immunodeficient mice of subpopulations of the stem/progenitor cells from human bone marrow referred to as either mesenchymal stem cells or marrow stromal cells (MSCs). The engraftment into adult mice without induced tissue injury was low and variable, but there was preferential engraftment of a subpopulation of rapidly self-renewing MSCs (RS-MSCs) compared with a subpopulation of slowly renewing MSCs (SR-MSCs). After intravenous infusion, there was a tendency for the cells to engraft into the hippocampal region that was previously designated a “vascular niche.” Migration assays suggested that preferential engraftment of RS-MSCs was in part explained by their expression of CXCR4 and CX3R1, the receptors for SDF-1 and fractalkine.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood-2005-07-2701</identifier><identifier>PMID: 16278305</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Bone Marrow Cells - cytology ; Bone Marrow Cells - physiology ; Cell Proliferation ; Cell Transplantation ; Cells, Cultured ; CX3C Chemokine Receptor 1 ; Gene Expression Regulation - physiology ; Graft Survival - physiology ; Hippocampus - cytology ; Hippocampus - physiology ; Humans ; Mice ; Mice, SCID ; Receptors, CXCR4 - biosynthesis ; Receptors, CXCR4 - genetics ; Receptors, Cytokine - biosynthesis ; Receptors, Cytokine - genetics ; Receptors, HIV - biosynthesis ; Receptors, HIV - genetics ; Stromal Cells - cytology ; Stromal Cells - physiology ; Stromal Cells - transplantation</subject><ispartof>Blood, 2006-03, Vol.107 (5), p.2153-2161</ispartof><rights>2006 American Society of Hematology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-fc3cb3bbb21e616be9a983d58be76fb7dfbc5cc8d0a7fcd475772e4f17cb7ecd3</citedby><cites>FETCH-LOGICAL-c464t-fc3cb3bbb21e616be9a983d58be76fb7dfbc5cc8d0a7fcd475772e4f17cb7ecd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27933,27934</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16278305$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Ryang Hwa</creatorcontrib><creatorcontrib>Hsu, Shu Ching</creatorcontrib><creatorcontrib>Munoz, James</creatorcontrib><creatorcontrib>Jung, Jin Sup</creatorcontrib><creatorcontrib>Lee, Na Rea</creatorcontrib><creatorcontrib>Pochampally, Radhika</creatorcontrib><creatorcontrib>Prockop, Darwin J.</creatorcontrib><title>A subset of human rapidly self-renewing marrow stromal cells preferentially engraft in mice</title><title>Blood</title><addtitle>Blood</addtitle><description>Controversies have arisen as to whether adult stem cells or progenitor cells from bone marrow can engraft into nonhematopoietic tissues in vivo. 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After intravenous infusion, there was a tendency for the cells to engraft into the hippocampal region that was previously designated a “vascular niche.” Migration assays suggested that preferential engraftment of RS-MSCs was in part explained by their expression of CXCR4 and CX3R1, the receptors for SDF-1 and fractalkine.</description><subject>Animals</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - physiology</subject><subject>Cell Proliferation</subject><subject>Cell Transplantation</subject><subject>Cells, Cultured</subject><subject>CX3C Chemokine Receptor 1</subject><subject>Gene Expression Regulation - physiology</subject><subject>Graft Survival - physiology</subject><subject>Hippocampus - cytology</subject><subject>Hippocampus - physiology</subject><subject>Humans</subject><subject>Mice</subject><subject>Mice, SCID</subject><subject>Receptors, CXCR4 - biosynthesis</subject><subject>Receptors, CXCR4 - genetics</subject><subject>Receptors, Cytokine - biosynthesis</subject><subject>Receptors, Cytokine - genetics</subject><subject>Receptors, HIV - biosynthesis</subject><subject>Receptors, HIV - genetics</subject><subject>Stromal Cells - cytology</subject><subject>Stromal Cells - physiology</subject><subject>Stromal Cells - transplantation</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1v2zAURYkiRe24_QMdAk7Z2D5SEikDWQwjbQME6NJOHQh-PLoMJNEhpQT-95FjA9kyveXci_sOIV85fOO8Fd9tl5JnAqBhoJhQwD-QJW9EywAEXJAlAEhWrxVfkMtSHgB4XYnmE1lwKVRbQbMk_za0TLbgSFOg_6feDDSbffTdgRbsAss44HMcdrQ3OadnWsacetNRh11X6D5jwBkZo-nmBA67bMJI40D76PAz-RhMV_DL-a7I3x-3f7a_2P3vn3fbzT1ztaxHFlzlbGWtFRwllxbXZt1WvmktKhms8sG6xrnWg1HB-Vo1SgmsA1fOKnS-WpHrU-8-p8cJy6j7WI4DzYBpKloq2VbrRs6gOIEup1Lm7Xqf4_zYQXPQR6X6Vak-KtWg9FHpHLo6t0-2R_8WOTucgZsTgPOPTxGzLi7i4NDHjG7UPsX3-l8AQ-6KRw</recordid><startdate>20060301</startdate><enddate>20060301</enddate><creator>Lee, Ryang Hwa</creator><creator>Hsu, Shu Ching</creator><creator>Munoz, James</creator><creator>Jung, Jin Sup</creator><creator>Lee, Na Rea</creator><creator>Pochampally, Radhika</creator><creator>Prockop, Darwin J.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060301</creationdate><title>A subset of human rapidly self-renewing marrow stromal cells preferentially engraft in mice</title><author>Lee, Ryang Hwa ; 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Bone Marrow Cells - cytology Bone Marrow Cells - physiology Cell Proliferation Cell Transplantation Cells, Cultured CX3C Chemokine Receptor 1 Gene Expression Regulation - physiology Graft Survival - physiology Hippocampus - cytology Hippocampus - physiology Humans Mice Mice, SCID Receptors, CXCR4 - biosynthesis Receptors, CXCR4 - genetics Receptors, Cytokine - biosynthesis Receptors, Cytokine - genetics Receptors, HIV - biosynthesis Receptors, HIV - genetics Stromal Cells - cytology Stromal Cells - physiology Stromal Cells - transplantation |
| title | A subset of human rapidly self-renewing marrow stromal cells preferentially engraft in mice |
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