Contribution of cell surface protein antigen PAc of Streptococcus mutans to bacteremia
Streptococcus mutans, a major cariogenic bacterium, is occasionally isolated from the blood of patients with bacteremia and infective endocarditis. Mutant strains of S. mutans MT8148, defective in the major surface proteins glucosyltransferase (GTF) B-, C-, and D-, and protein antigen c (PAc), were...
Gespeichert in:
| Veröffentlicht in: | Microbes and infection 2006, Vol.8 (1), p.114-121 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 121 |
|---|---|
| container_issue | 1 |
| container_start_page | 114 |
| container_title | Microbes and infection |
| container_volume | 8 |
| creator | Nakano, Kazuhiko Tsuji, Masato Nishimura, Kaoru Nomura, Ryota Ooshima, Takashi |
| description | Streptococcus mutans, a major cariogenic bacterium, is occasionally isolated from the blood of patients with bacteremia and infective endocarditis. Mutant strains of
S. mutans MT8148, defective in the major surface proteins glucosyltransferase (GTF) B-, C-, and D-, and protein antigen c (PAc), were constructed by insertional inactivation of each respective gene with an antibiotic resistant cassette. Susceptibility to phagocytosis was determined by analyses of interactions of the bacteria with human polymorphonuclear leukocytes, and the PAc-defective mutant strain (PD) showed the lowest rate of phagocytosis. Further, when PD and MT8148 were separately injected into the jugular veins of Sprague–Dawley rats, PD was recovered in significantly larger numbers and for a longer duration, and caused more severe systemic inflammation than MT8148, indicating that
S. mutans PAc is associated with its systemic virulence in blood. Next, 100
S. mutans clinical isolates from 100 Japanese children and adolescents were analyzed by Western blotting using antisera raised against recombinant PAc, generated based on the
pac sequence of MT8148. Four of the 100 strains showed no positive band and each exhibited a significantly lower phagocytosis rate than that of 25 randomly selected clinical strains (
P
<
0.01). In addition, three of the 100 strains possessed a lower molecular weight PAc and a significantly lower rate of phagocytosis than the 25 reference strains (
P
<
0.05). These results suggest that
S. mutans PAc may be associated with phagocytosis susceptibility to human polymorphonuclear leukocytes, with approximately 7% of
S. mutans clinical isolates possible high-risk strains for the development of bacteremia. |
| doi_str_mv | 10.1016/j.micinf.2005.06.005 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67681220</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1286457905002315</els_id><sourcerecordid>20966911</sourcerecordid><originalsourceid>FETCH-LOGICAL-c533t-7e26b676da9a9b623a2fa9f8a9ff1b21f1b5afaea3e360f7120dea195f25d1373</originalsourceid><addsrcrecordid>eNqFkE9r3DAQxUVpadK03yAEXdqbXUm2pdUlEJb-g0ALbUpvYiyPgpa1tJHkQr99ZHYht_Qw83T4zePpEXLJWcsZlx937eytD64VjA0tk22VF-ScK6kbxfs_L-tbbGTTD0qfkTc57xjjg5L9a3LGZd-LfiPPye9tDCX5cSk-Bhodtbjf07wkBxbpIcWCPlAIxd9joD9u7Mr8LAkPJdpo7ZLpvBQImZZIR7AFE84e3pJXDvYZ3530gtx9_vRr-7W5_f7l2_bmtrFD15VGoZCjVHICDXqUogPhQLtNHcdHwesawAFCh51kTnHBJgSuByeGiXequyAfjr416cOCuZjZ5_ULEDAu2VTvDReC_RcUTEupOa9gfwRtijkndOaQ_Azpn-HMrMWbnTkWb9biDZOmSj27Ovkv44zT09Gp6Qq8PwGQLexdgmB9fuJUr5ju1qDXRw5rbX89JpOtx2Bx8gltMVP0zyd5BEVPpBQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20966911</pqid></control><display><type>article</type><title>Contribution of cell surface protein antigen PAc of Streptococcus mutans to bacteremia</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Nakano, Kazuhiko ; Tsuji, Masato ; Nishimura, Kaoru ; Nomura, Ryota ; Ooshima, Takashi</creator><creatorcontrib>Nakano, Kazuhiko ; Tsuji, Masato ; Nishimura, Kaoru ; Nomura, Ryota ; Ooshima, Takashi</creatorcontrib><description>Streptococcus mutans, a major cariogenic bacterium, is occasionally isolated from the blood of patients with bacteremia and infective endocarditis. Mutant strains of
S. mutans MT8148, defective in the major surface proteins glucosyltransferase (GTF) B-, C-, and D-, and protein antigen c (PAc), were constructed by insertional inactivation of each respective gene with an antibiotic resistant cassette. Susceptibility to phagocytosis was determined by analyses of interactions of the bacteria with human polymorphonuclear leukocytes, and the PAc-defective mutant strain (PD) showed the lowest rate of phagocytosis. Further, when PD and MT8148 were separately injected into the jugular veins of Sprague–Dawley rats, PD was recovered in significantly larger numbers and for a longer duration, and caused more severe systemic inflammation than MT8148, indicating that
S. mutans PAc is associated with its systemic virulence in blood. Next, 100
S. mutans clinical isolates from 100 Japanese children and adolescents were analyzed by Western blotting using antisera raised against recombinant PAc, generated based on the
pac sequence of MT8148. Four of the 100 strains showed no positive band and each exhibited a significantly lower phagocytosis rate than that of 25 randomly selected clinical strains (
P
<
0.01). In addition, three of the 100 strains possessed a lower molecular weight PAc and a significantly lower rate of phagocytosis than the 25 reference strains (
P
<
0.05). These results suggest that
S. mutans PAc may be associated with phagocytosis susceptibility to human polymorphonuclear leukocytes, with approximately 7% of
S. mutans clinical isolates possible high-risk strains for the development of bacteremia.</description><identifier>ISSN: 1286-4579</identifier><identifier>EISSN: 1769-714X</identifier><identifier>DOI: 10.1016/j.micinf.2005.06.005</identifier><identifier>PMID: 16442486</identifier><language>eng</language><publisher>Lausanne: Elsevier SAS</publisher><subject>Animals ; Antigens, Bacterial - genetics ; Antigens, Bacterial - metabolism ; Bacteremia ; Bacteremia - microbiology ; Bacterial diseases ; Bacterial sepsis ; Bacteriology ; Biological and medical sciences ; Clinical strains ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial ; Human bacterial diseases ; Infectious diseases ; Infective endocarditis ; Medical sciences ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Microbiology ; Miscellaneous ; Mutation ; Phagocytosis ; Protein antigen c ; Rat model ; Rats ; Streptococcal Infections - microbiology ; Streptococcus mutans ; Streptococcus mutans - genetics ; Streptococcus mutans - metabolism ; Streptococcus mutans - pathogenicity ; Time Factors ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Virulence</subject><ispartof>Microbes and infection, 2006, Vol.8 (1), p.114-121</ispartof><rights>2005 Elsevier SAS</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c533t-7e26b676da9a9b623a2fa9f8a9ff1b21f1b5afaea3e360f7120dea195f25d1373</citedby><cites>FETCH-LOGICAL-c533t-7e26b676da9a9b623a2fa9f8a9ff1b21f1b5afaea3e360f7120dea195f25d1373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.micinf.2005.06.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17470930$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16442486$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakano, Kazuhiko</creatorcontrib><creatorcontrib>Tsuji, Masato</creatorcontrib><creatorcontrib>Nishimura, Kaoru</creatorcontrib><creatorcontrib>Nomura, Ryota</creatorcontrib><creatorcontrib>Ooshima, Takashi</creatorcontrib><title>Contribution of cell surface protein antigen PAc of Streptococcus mutans to bacteremia</title><title>Microbes and infection</title><addtitle>Microbes Infect</addtitle><description>Streptococcus mutans, a major cariogenic bacterium, is occasionally isolated from the blood of patients with bacteremia and infective endocarditis. Mutant strains of
S. mutans MT8148, defective in the major surface proteins glucosyltransferase (GTF) B-, C-, and D-, and protein antigen c (PAc), were constructed by insertional inactivation of each respective gene with an antibiotic resistant cassette. Susceptibility to phagocytosis was determined by analyses of interactions of the bacteria with human polymorphonuclear leukocytes, and the PAc-defective mutant strain (PD) showed the lowest rate of phagocytosis. Further, when PD and MT8148 were separately injected into the jugular veins of Sprague–Dawley rats, PD was recovered in significantly larger numbers and for a longer duration, and caused more severe systemic inflammation than MT8148, indicating that
S. mutans PAc is associated with its systemic virulence in blood. Next, 100
S. mutans clinical isolates from 100 Japanese children and adolescents were analyzed by Western blotting using antisera raised against recombinant PAc, generated based on the
pac sequence of MT8148. Four of the 100 strains showed no positive band and each exhibited a significantly lower phagocytosis rate than that of 25 randomly selected clinical strains (
P
<
0.01). In addition, three of the 100 strains possessed a lower molecular weight PAc and a significantly lower rate of phagocytosis than the 25 reference strains (
P
<
0.05). These results suggest that
S. mutans PAc may be associated with phagocytosis susceptibility to human polymorphonuclear leukocytes, with approximately 7% of
S. mutans clinical isolates possible high-risk strains for the development of bacteremia.</description><subject>Animals</subject><subject>Antigens, Bacterial - genetics</subject><subject>Antigens, Bacterial - metabolism</subject><subject>Bacteremia</subject><subject>Bacteremia - microbiology</subject><subject>Bacterial diseases</subject><subject>Bacterial sepsis</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Clinical strains</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Human bacterial diseases</subject><subject>Infectious diseases</subject><subject>Infective endocarditis</subject><subject>Medical sciences</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mutation</subject><subject>Phagocytosis</subject><subject>Protein antigen c</subject><subject>Rat model</subject><subject>Rats</subject><subject>Streptococcal Infections - microbiology</subject><subject>Streptococcus mutans</subject><subject>Streptococcus mutans - genetics</subject><subject>Streptococcus mutans - metabolism</subject><subject>Streptococcus mutans - pathogenicity</subject><subject>Time Factors</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Virulence</subject><issn>1286-4579</issn><issn>1769-714X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9r3DAQxUVpadK03yAEXdqbXUm2pdUlEJb-g0ALbUpvYiyPgpa1tJHkQr99ZHYht_Qw83T4zePpEXLJWcsZlx937eytD64VjA0tk22VF-ScK6kbxfs_L-tbbGTTD0qfkTc57xjjg5L9a3LGZd-LfiPPye9tDCX5cSk-Bhodtbjf07wkBxbpIcWCPlAIxd9joD9u7Mr8LAkPJdpo7ZLpvBQImZZIR7AFE84e3pJXDvYZ3530gtx9_vRr-7W5_f7l2_bmtrFD15VGoZCjVHICDXqUogPhQLtNHcdHwesawAFCh51kTnHBJgSuByeGiXequyAfjr416cOCuZjZ5_ULEDAu2VTvDReC_RcUTEupOa9gfwRtijkndOaQ_Azpn-HMrMWbnTkWb9biDZOmSj27Ovkv44zT09Gp6Qq8PwGQLexdgmB9fuJUr5ju1qDXRw5rbX89JpOtx2Bx8gltMVP0zyd5BEVPpBQ</recordid><startdate>2006</startdate><enddate>2006</enddate><creator>Nakano, Kazuhiko</creator><creator>Tsuji, Masato</creator><creator>Nishimura, Kaoru</creator><creator>Nomura, Ryota</creator><creator>Ooshima, Takashi</creator><general>Elsevier SAS</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>2006</creationdate><title>Contribution of cell surface protein antigen PAc of Streptococcus mutans to bacteremia</title><author>Nakano, Kazuhiko ; Tsuji, Masato ; Nishimura, Kaoru ; Nomura, Ryota ; Ooshima, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c533t-7e26b676da9a9b623a2fa9f8a9ff1b21f1b5afaea3e360f7120dea195f25d1373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Antigens, Bacterial - genetics</topic><topic>Antigens, Bacterial - metabolism</topic><topic>Bacteremia</topic><topic>Bacteremia - microbiology</topic><topic>Bacterial diseases</topic><topic>Bacterial sepsis</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Clinical strains</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Human bacterial diseases</topic><topic>Infectious diseases</topic><topic>Infective endocarditis</topic><topic>Medical sciences</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mutation</topic><topic>Phagocytosis</topic><topic>Protein antigen c</topic><topic>Rat model</topic><topic>Rats</topic><topic>Streptococcal Infections - microbiology</topic><topic>Streptococcus mutans</topic><topic>Streptococcus mutans - genetics</topic><topic>Streptococcus mutans - metabolism</topic><topic>Streptococcus mutans - pathogenicity</topic><topic>Time Factors</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakano, Kazuhiko</creatorcontrib><creatorcontrib>Tsuji, Masato</creatorcontrib><creatorcontrib>Nishimura, Kaoru</creatorcontrib><creatorcontrib>Nomura, Ryota</creatorcontrib><creatorcontrib>Ooshima, Takashi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Microbes and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakano, Kazuhiko</au><au>Tsuji, Masato</au><au>Nishimura, Kaoru</au><au>Nomura, Ryota</au><au>Ooshima, Takashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Contribution of cell surface protein antigen PAc of Streptococcus mutans to bacteremia</atitle><jtitle>Microbes and infection</jtitle><addtitle>Microbes Infect</addtitle><date>2006</date><risdate>2006</risdate><volume>8</volume><issue>1</issue><spage>114</spage><epage>121</epage><pages>114-121</pages><issn>1286-4579</issn><eissn>1769-714X</eissn><abstract>Streptococcus mutans, a major cariogenic bacterium, is occasionally isolated from the blood of patients with bacteremia and infective endocarditis. Mutant strains of
S. mutans MT8148, defective in the major surface proteins glucosyltransferase (GTF) B-, C-, and D-, and protein antigen c (PAc), were constructed by insertional inactivation of each respective gene with an antibiotic resistant cassette. Susceptibility to phagocytosis was determined by analyses of interactions of the bacteria with human polymorphonuclear leukocytes, and the PAc-defective mutant strain (PD) showed the lowest rate of phagocytosis. Further, when PD and MT8148 were separately injected into the jugular veins of Sprague–Dawley rats, PD was recovered in significantly larger numbers and for a longer duration, and caused more severe systemic inflammation than MT8148, indicating that
S. mutans PAc is associated with its systemic virulence in blood. Next, 100
S. mutans clinical isolates from 100 Japanese children and adolescents were analyzed by Western blotting using antisera raised against recombinant PAc, generated based on the
pac sequence of MT8148. Four of the 100 strains showed no positive band and each exhibited a significantly lower phagocytosis rate than that of 25 randomly selected clinical strains (
P
<
0.01). In addition, three of the 100 strains possessed a lower molecular weight PAc and a significantly lower rate of phagocytosis than the 25 reference strains (
P
<
0.05). These results suggest that
S. mutans PAc may be associated with phagocytosis susceptibility to human polymorphonuclear leukocytes, with approximately 7% of
S. mutans clinical isolates possible high-risk strains for the development of bacteremia.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>Paris</cop><pub>Elsevier SAS</pub><pmid>16442486</pmid><doi>10.1016/j.micinf.2005.06.005</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1286-4579 |
| ispartof | Microbes and infection, 2006, Vol.8 (1), p.114-121 |
| issn | 1286-4579 1769-714X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67681220 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Antigens, Bacterial - genetics Antigens, Bacterial - metabolism Bacteremia Bacteremia - microbiology Bacterial diseases Bacterial sepsis Bacteriology Biological and medical sciences Clinical strains DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Human bacterial diseases Infectious diseases Infective endocarditis Medical sciences Membrane Proteins - genetics Membrane Proteins - metabolism Microbiology Miscellaneous Mutation Phagocytosis Protein antigen c Rat model Rats Streptococcal Infections - microbiology Streptococcus mutans Streptococcus mutans - genetics Streptococcus mutans - metabolism Streptococcus mutans - pathogenicity Time Factors Transcription Factors - genetics Transcription Factors - metabolism Virulence |
| title | Contribution of cell surface protein antigen PAc of Streptococcus mutans to bacteremia |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T20%3A44%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Contribution%20of%20cell%20surface%20protein%20antigen%20PAc%20of%20Streptococcus%20mutans%20to%20bacteremia&rft.jtitle=Microbes%20and%20infection&rft.au=Nakano,%20Kazuhiko&rft.date=2006&rft.volume=8&rft.issue=1&rft.spage=114&rft.epage=121&rft.pages=114-121&rft.issn=1286-4579&rft.eissn=1769-714X&rft_id=info:doi/10.1016/j.micinf.2005.06.005&rft_dat=%3Cproquest_cross%3E20966911%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20966911&rft_id=info:pmid/16442486&rft_els_id=S1286457905002315&rfr_iscdi=true |