Moment analysis for kinetics of gene silencing by RNA interference
RNA interference (RNAi) was quantitatively evaluated from a kinetic viewpoint. A simple kinetic evaluation based on moment analysis was proposed, assuming suppression and recovery phases of gene expression. We defined the area under the curve of the inhibitory effect (AUCIE) as an index of the total...
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| Veröffentlicht in: | Biotechnology and bioengineering 2006-03, Vol.93 (4), p.816-819 |
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| container_title | Biotechnology and bioengineering |
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| creator | Takahashi, Yuki Yamaoka, Kiyoshi Nishikawa, Makiya Takakura, Yoshinobu |
| description | RNA interference (RNAi) was quantitatively evaluated from a kinetic viewpoint. A simple kinetic evaluation based on moment analysis was proposed, assuming suppression and recovery phases of gene expression. We defined the area under the curve of the inhibitory effect (AUCIE) as an index of the total intensity of RNAi and the mean response time of the inhibitory effect (MRTIE) as an index of its duration. The proposed kinetic analysis helps to understand the RNAi effect in a quantitative and time‐dependent manner, which will be beneficial for designing RNAi‐based gene silencing for both experimental and therapeutic purposes. © 2005 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/bit.20718 |
| format | Article |
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Bioeng</addtitle><description>RNA interference (RNAi) was quantitatively evaluated from a kinetic viewpoint. A simple kinetic evaluation based on moment analysis was proposed, assuming suppression and recovery phases of gene expression. We defined the area under the curve of the inhibitory effect (AUCIE) as an index of the total intensity of RNAi and the mean response time of the inhibitory effect (MRTIE) as an index of its duration. The proposed kinetic analysis helps to understand the RNAi effect in a quantitative and time‐dependent manner, which will be beneficial for designing RNAi‐based gene silencing for both experimental and therapeutic purposes. © 2005 Wiley Periodicals, Inc.</description><subject>Animals</subject><subject>Area Under Curve</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Line, Tumor</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>gene silencing</subject><subject>Kinetics</subject><subject>luciferase</subject><subject>Luciferases, Firefly - genetics</subject><subject>Luciferases, Firefly - metabolism</subject><subject>Luciferases, Renilla - genetics</subject><subject>Luciferases, Renilla - metabolism</subject><subject>Mice</subject><subject>moment analysis</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Interference</subject><subject>RNA, Small Interfering - genetics</subject><subject>Transfection</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkNFqFDEUhoModl298AUkCAq9mPYkmSSTy7ZqLWwrSKXQm5DJnJS0szM1mUX37U272xYE8SokfOf_Tz5C3jLYYwB8v43THgfNmmdkxsDoCriB52QGAKoS0vAd8irn63LVjVIvyQ5TzCiQZkYOT8clDhN1g-vXOWYaxkRv4oBT9JmOgV7hgDTHHgcfhyvarun3swMahwlTwFRe8TV5EVyf8c32nJMfXz6fH32tFt-OT44OFpWXEpqKo-NKaEQXgBnXgkfoTMtkF4JXXSMZIi_LBnCydsyLYOqAna81dtxwJ-bk4yb3No0_V5gnu4zZY9-7AcdVtkqrpvxQ_hfkwGumNRTw_V_g9bhKxURhmNBKGHGXtruBfBpzThjsbYpLl9aWgb3Tb4t-e6-_sO-2gat2id0TufVdgA9bwGXv-pBc0ZqfOC1rJkvxnOxvuF_F_Prfjfbw5PyhutpMxDzh78cJl26KGKGlvTg7theXnxbNJWh7Kv4AeKep2g</recordid><startdate>20060305</startdate><enddate>20060305</enddate><creator>Takahashi, Yuki</creator><creator>Yamaoka, Kiyoshi</creator><creator>Nishikawa, Makiya</creator><creator>Takakura, Yoshinobu</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7TM</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060305</creationdate><title>Moment analysis for kinetics of gene silencing by RNA interference</title><author>Takahashi, Yuki ; Yamaoka, Kiyoshi ; Nishikawa, Makiya ; Takakura, Yoshinobu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5508-2ea2637eeaf019ab0ce0d9b15dffc6d851ee2359f0a54a1c3f94fedc47ed292a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Area Under Curve</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell Line, Tumor</topic><topic>Fundamental and applied biological sciences. 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Bioeng</addtitle><date>2006-03-05</date><risdate>2006</risdate><volume>93</volume><issue>4</issue><spage>816</spage><epage>819</epage><pages>816-819</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>RNA interference (RNAi) was quantitatively evaluated from a kinetic viewpoint. A simple kinetic evaluation based on moment analysis was proposed, assuming suppression and recovery phases of gene expression. We defined the area under the curve of the inhibitory effect (AUCIE) as an index of the total intensity of RNAi and the mean response time of the inhibitory effect (MRTIE) as an index of its duration. 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| subjects | Animals Area Under Curve Biological and medical sciences Biotechnology Cell Line, Tumor Fundamental and applied biological sciences. Psychology Gene expression gene silencing Kinetics luciferase Luciferases, Firefly - genetics Luciferases, Firefly - metabolism Luciferases, Renilla - genetics Luciferases, Renilla - metabolism Mice moment analysis Ribonucleic acid RNA RNA Interference RNA, Small Interfering - genetics Transfection |
| title | Moment analysis for kinetics of gene silencing by RNA interference |
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