Immunization with a DNA chimeric molecule encoding a hemagglutinin peptide and a scFv CD21-specific antibody fragment induces long-lasting IgM and CTL responses to influenza virus
Killed viral vaccines are known to induce primarily antibody responses. By contrast DNA vaccination using naked DNA encoding viral antigens induces both humoral and cellular immune responses. Various approaches have been used to construct DNA vaccines with build-in adjuvanticity. We hypothesized tha...
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Veröffentlicht in: | Vaccine 2006-03, Vol.24 (11), p.1830-1837 |
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creator | Ivanovska, Nina Tchorbanov, Andrey Prechl, Jozsef Maximova, Vera Voynova, Elisaveta Vassilev, Tchavdar L. |
description | Killed viral vaccines are known to induce primarily antibody responses. By contrast DNA vaccination using naked DNA encoding viral antigens induces both humoral and cellular immune responses. Various approaches have been used to construct DNA vaccines with build-in adjuvanticity. We hypothesized that sequences encoding a common epitope of influenza A virus hemagglutinin jointed to sequences encoding a single-chain variable fragment (scFv) antibody fragment to a costimulatory B cell surface receptor would result in the in vivo expression of a chimeric viral peptide with increased immunogenicity. Such a hybrid DNA molecule was constructed by us, encoding a T and B cell epitope-containing influenza hemagglutinin peptide and a scFv antibody fragment binding to mouse complement receptors I and II (CR1 and CR2). A single immunization with a plasmid containing the described construct induced a strong anti-influenza cytotoxic response lasting for more than six months and a weak antibody response. |
doi_str_mv | 10.1016/j.vaccine.2005.10.029 |
format | Article |
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By contrast DNA vaccination using naked DNA encoding viral antigens induces both humoral and cellular immune responses. Various approaches have been used to construct DNA vaccines with build-in adjuvanticity. We hypothesized that sequences encoding a common epitope of influenza A virus hemagglutinin jointed to sequences encoding a single-chain variable fragment (scFv) antibody fragment to a costimulatory B cell surface receptor would result in the in vivo expression of a chimeric viral peptide with increased immunogenicity. Such a hybrid DNA molecule was constructed by us, encoding a T and B cell epitope-containing influenza hemagglutinin peptide and a scFv antibody fragment binding to mouse complement receptors I and II (CR1 and CR2). A single immunization with a plasmid containing the described construct induced a strong anti-influenza cytotoxic response lasting for more than six months and a weak antibody response.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2005.10.029</identifier><identifier>PMID: 16289708</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adjuvants, Immunologic ; Animals ; Antibodies, Viral - blood ; Antigens ; Applied microbiology ; Biological and medical sciences ; CTL response ; Cytokines ; Cytotoxicity ; Cytotoxicity, Immunologic ; Deoxyribonucleic acid ; DNA ; DNA vaccines ; Enzyme-Linked Immunosorbent Assay ; Female ; Fundamental and applied biological sciences. Psychology ; Hemagglutination Inhibition Tests ; Hemagglutinin Glycoproteins, Influenza Virus - genetics ; Hemagglutinin Glycoproteins, Influenza Virus - immunology ; Immune system ; Immunization ; Immunogenicity ; Immunoglobulin G - blood ; Immunoglobulin M - blood ; Immunoglobulin Variable Region - genetics ; Influenza A virus ; Influenza A virus - immunology ; Influenza Vaccines - immunology ; Influenza virus ; Mice ; Mice, Inbred BALB C ; Microbiology ; Miscellaneous ; Peptides ; Plasmids ; Proteins ; Receptors, Complement - immunology ; Receptors, Complement 3d - immunology ; Rodents ; T-Lymphocytes, Cytotoxic - immunology ; Vaccination ; Vaccines ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vaccines, DNA - administration & dosage ; Vaccines, DNA - genetics ; Vaccines, DNA - immunology ; Virology</subject><ispartof>Vaccine, 2006-03, Vol.24 (11), p.1830-1837</ispartof><rights>2005 Elsevier Ltd</rights><rights>2006 INIST-CNRS</rights><rights>Copyright Elsevier Limited Mar 10, 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-e73910fcafd0f5daad929aa0e81c09ed816f4664d4cbd9a83c73162457283ed23</citedby><cites>FETCH-LOGICAL-c452t-e73910fcafd0f5daad929aa0e81c09ed816f4664d4cbd9a83c73162457283ed23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1547142563?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994,64384,64386,64388,72340</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17582539$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16289708$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ivanovska, Nina</creatorcontrib><creatorcontrib>Tchorbanov, Andrey</creatorcontrib><creatorcontrib>Prechl, Jozsef</creatorcontrib><creatorcontrib>Maximova, Vera</creatorcontrib><creatorcontrib>Voynova, Elisaveta</creatorcontrib><creatorcontrib>Vassilev, Tchavdar L.</creatorcontrib><title>Immunization with a DNA chimeric molecule encoding a hemagglutinin peptide and a scFv CD21-specific antibody fragment induces long-lasting IgM and CTL responses to influenza virus</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Killed viral vaccines are known to induce primarily antibody responses. By contrast DNA vaccination using naked DNA encoding viral antigens induces both humoral and cellular immune responses. Various approaches have been used to construct DNA vaccines with build-in adjuvanticity. We hypothesized that sequences encoding a common epitope of influenza A virus hemagglutinin jointed to sequences encoding a single-chain variable fragment (scFv) antibody fragment to a costimulatory B cell surface receptor would result in the in vivo expression of a chimeric viral peptide with increased immunogenicity. Such a hybrid DNA molecule was constructed by us, encoding a T and B cell epitope-containing influenza hemagglutinin peptide and a scFv antibody fragment binding to mouse complement receptors I and II (CR1 and CR2). A single immunization with a plasmid containing the described construct induced a strong anti-influenza cytotoxic response lasting for more than six months and a weak antibody response.</description><subject>Adjuvants, Immunologic</subject><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>Antigens</subject><subject>Applied microbiology</subject><subject>Biological and medical sciences</subject><subject>CTL response</subject><subject>Cytokines</subject><subject>Cytotoxicity</subject><subject>Cytotoxicity, Immunologic</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA vaccines</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemagglutination Inhibition Tests</subject><subject>Hemagglutinin Glycoproteins, Influenza Virus - genetics</subject><subject>Hemagglutinin Glycoproteins, Influenza Virus - immunology</subject><subject>Immune system</subject><subject>Immunization</subject><subject>Immunogenicity</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin M - blood</subject><subject>Immunoglobulin Variable Region - genetics</subject><subject>Influenza A virus</subject><subject>Influenza A virus - immunology</subject><subject>Influenza Vaccines - immunology</subject><subject>Influenza virus</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Peptides</subject><subject>Plasmids</subject><subject>Proteins</subject><subject>Receptors, Complement - immunology</subject><subject>Receptors, Complement 3d - immunology</subject><subject>Rodents</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><subject>Vaccination</subject><subject>Vaccines</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Vaccines, DNA - administration & dosage</subject><subject>Vaccines, DNA - genetics</subject><subject>Vaccines, DNA - immunology</subject><subject>Virology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkdGK1DAUhoso7rj6CEpA9K5jkrZJeyXLrKsDo96s4F3IJKedDG1Sk3Rk97V8QVOnsODNXgVOvvOT_F-WvSZ4TTBhH47rk1TKWFhTjKs0W2PaPMlWpOZFTitSP81WmLIyLwn-eZG9COGIE1iQ5nl2QRitG47rVfZnOwyTNfcyGmfRbxMPSKLrb1dIHcwA3ig0uB7U1AMCq5w2tkvAAQbZdf0UjTUWjTBGowFJq9NdUDcntLmmJA8jKNOmCGmj2Tt9h1ovuwFsRMbqSUFAvbNd3ssQ59xt9_VfxuZ2hzyE0dmQkOgS3fYT2HuJTsZP4WX2rJV9gFfLeZn9uPl0u_mS775_3m6udrkqKxpz4EVDcKtkq3FbaSl1QxspMdRE4QZ0TVhbMlbqUu11I-tC8SIVU1ac1gVoWlxm78-5o3e_JghRDCYo6HtpwU1BMM54apE8ChJeclaTGXz7H3h0k7fpE4JUJSclrViRqOpMKe9C8NCK0ZtB-jtBsJjli6NY5ItZ_jxO8tPemyV92g-gH7YW2wl4twAyKNknG1aZ8MDxqqZVMQd9PHOQ2j0Z8CIok_SDNh5UFNqZR57yFxhu0ek</recordid><startdate>20060310</startdate><enddate>20060310</enddate><creator>Ivanovska, Nina</creator><creator>Tchorbanov, Andrey</creator><creator>Prechl, Jozsef</creator><creator>Maximova, Vera</creator><creator>Voynova, Elisaveta</creator><creator>Vassilev, Tchavdar L.</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060310</creationdate><title>Immunization with a DNA chimeric molecule encoding a hemagglutinin peptide and a scFv CD21-specific antibody fragment induces long-lasting IgM and CTL responses to influenza virus</title><author>Ivanovska, Nina ; Tchorbanov, Andrey ; Prechl, Jozsef ; Maximova, Vera ; Voynova, Elisaveta ; Vassilev, Tchavdar L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-e73910fcafd0f5daad929aa0e81c09ed816f4664d4cbd9a83c73162457283ed23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adjuvants, Immunologic</topic><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>Antigens</topic><topic>Applied microbiology</topic><topic>Biological and medical sciences</topic><topic>CTL response</topic><topic>Cytokines</topic><topic>Cytotoxicity</topic><topic>Cytotoxicity, Immunologic</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA vaccines</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemagglutination Inhibition Tests</topic><topic>Hemagglutinin Glycoproteins, Influenza Virus - genetics</topic><topic>Hemagglutinin Glycoproteins, Influenza Virus - immunology</topic><topic>Immune system</topic><topic>Immunization</topic><topic>Immunogenicity</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulin M - blood</topic><topic>Immunoglobulin Variable Region - genetics</topic><topic>Influenza A virus</topic><topic>Influenza A virus - immunology</topic><topic>Influenza Vaccines - immunology</topic><topic>Influenza virus</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Peptides</topic><topic>Plasmids</topic><topic>Proteins</topic><topic>Receptors, Complement - immunology</topic><topic>Receptors, Complement 3d - immunology</topic><topic>Rodents</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><topic>Vaccination</topic><topic>Vaccines</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Vaccines, DNA - 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Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ivanovska, Nina</au><au>Tchorbanov, Andrey</au><au>Prechl, Jozsef</au><au>Maximova, Vera</au><au>Voynova, Elisaveta</au><au>Vassilev, Tchavdar L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunization with a DNA chimeric molecule encoding a hemagglutinin peptide and a scFv CD21-specific antibody fragment induces long-lasting IgM and CTL responses to influenza virus</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2006-03-10</date><risdate>2006</risdate><volume>24</volume><issue>11</issue><spage>1830</spage><epage>1837</epage><pages>1830-1837</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Killed viral vaccines are known to induce primarily antibody responses. By contrast DNA vaccination using naked DNA encoding viral antigens induces both humoral and cellular immune responses. Various approaches have been used to construct DNA vaccines with build-in adjuvanticity. We hypothesized that sequences encoding a common epitope of influenza A virus hemagglutinin jointed to sequences encoding a single-chain variable fragment (scFv) antibody fragment to a costimulatory B cell surface receptor would result in the in vivo expression of a chimeric viral peptide with increased immunogenicity. Such a hybrid DNA molecule was constructed by us, encoding a T and B cell epitope-containing influenza hemagglutinin peptide and a scFv antibody fragment binding to mouse complement receptors I and II (CR1 and CR2). A single immunization with a plasmid containing the described construct induced a strong anti-influenza cytotoxic response lasting for more than six months and a weak antibody response.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>16289708</pmid><doi>10.1016/j.vaccine.2005.10.029</doi><tpages>8</tpages></addata></record> |
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subjects | Adjuvants, Immunologic Animals Antibodies, Viral - blood Antigens Applied microbiology Biological and medical sciences CTL response Cytokines Cytotoxicity Cytotoxicity, Immunologic Deoxyribonucleic acid DNA DNA vaccines Enzyme-Linked Immunosorbent Assay Female Fundamental and applied biological sciences. Psychology Hemagglutination Inhibition Tests Hemagglutinin Glycoproteins, Influenza Virus - genetics Hemagglutinin Glycoproteins, Influenza Virus - immunology Immune system Immunization Immunogenicity Immunoglobulin G - blood Immunoglobulin M - blood Immunoglobulin Variable Region - genetics Influenza A virus Influenza A virus - immunology Influenza Vaccines - immunology Influenza virus Mice Mice, Inbred BALB C Microbiology Miscellaneous Peptides Plasmids Proteins Receptors, Complement - immunology Receptors, Complement 3d - immunology Rodents T-Lymphocytes, Cytotoxic - immunology Vaccination Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, DNA - administration & dosage Vaccines, DNA - genetics Vaccines, DNA - immunology Virology |
title | Immunization with a DNA chimeric molecule encoding a hemagglutinin peptide and a scFv CD21-specific antibody fragment induces long-lasting IgM and CTL responses to influenza virus |
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