Immunization with a DNA chimeric molecule encoding a hemagglutinin peptide and a scFv CD21-specific antibody fragment induces long-lasting IgM and CTL responses to influenza virus

Killed viral vaccines are known to induce primarily antibody responses. By contrast DNA vaccination using naked DNA encoding viral antigens induces both humoral and cellular immune responses. Various approaches have been used to construct DNA vaccines with build-in adjuvanticity. We hypothesized tha...

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Veröffentlicht in:Vaccine 2006-03, Vol.24 (11), p.1830-1837
Hauptverfasser: Ivanovska, Nina, Tchorbanov, Andrey, Prechl, Jozsef, Maximova, Vera, Voynova, Elisaveta, Vassilev, Tchavdar L.
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container_end_page 1837
container_issue 11
container_start_page 1830
container_title Vaccine
container_volume 24
creator Ivanovska, Nina
Tchorbanov, Andrey
Prechl, Jozsef
Maximova, Vera
Voynova, Elisaveta
Vassilev, Tchavdar L.
description Killed viral vaccines are known to induce primarily antibody responses. By contrast DNA vaccination using naked DNA encoding viral antigens induces both humoral and cellular immune responses. Various approaches have been used to construct DNA vaccines with build-in adjuvanticity. We hypothesized that sequences encoding a common epitope of influenza A virus hemagglutinin jointed to sequences encoding a single-chain variable fragment (scFv) antibody fragment to a costimulatory B cell surface receptor would result in the in vivo expression of a chimeric viral peptide with increased immunogenicity. Such a hybrid DNA molecule was constructed by us, encoding a T and B cell epitope-containing influenza hemagglutinin peptide and a scFv antibody fragment binding to mouse complement receptors I and II (CR1 and CR2). A single immunization with a plasmid containing the described construct induced a strong anti-influenza cytotoxic response lasting for more than six months and a weak antibody response.
doi_str_mv 10.1016/j.vaccine.2005.10.029
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By contrast DNA vaccination using naked DNA encoding viral antigens induces both humoral and cellular immune responses. Various approaches have been used to construct DNA vaccines with build-in adjuvanticity. We hypothesized that sequences encoding a common epitope of influenza A virus hemagglutinin jointed to sequences encoding a single-chain variable fragment (scFv) antibody fragment to a costimulatory B cell surface receptor would result in the in vivo expression of a chimeric viral peptide with increased immunogenicity. Such a hybrid DNA molecule was constructed by us, encoding a T and B cell epitope-containing influenza hemagglutinin peptide and a scFv antibody fragment binding to mouse complement receptors I and II (CR1 and CR2). 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subjects Adjuvants, Immunologic
Animals
Antibodies, Viral - blood
Antigens
Applied microbiology
Biological and medical sciences
CTL response
Cytokines
Cytotoxicity
Cytotoxicity, Immunologic
Deoxyribonucleic acid
DNA
DNA vaccines
Enzyme-Linked Immunosorbent Assay
Female
Fundamental and applied biological sciences. Psychology
Hemagglutination Inhibition Tests
Hemagglutinin Glycoproteins, Influenza Virus - genetics
Hemagglutinin Glycoproteins, Influenza Virus - immunology
Immune system
Immunization
Immunogenicity
Immunoglobulin G - blood
Immunoglobulin M - blood
Immunoglobulin Variable Region - genetics
Influenza A virus
Influenza A virus - immunology
Influenza Vaccines - immunology
Influenza virus
Mice
Mice, Inbred BALB C
Microbiology
Miscellaneous
Peptides
Plasmids
Proteins
Receptors, Complement - immunology
Receptors, Complement 3d - immunology
Rodents
T-Lymphocytes, Cytotoxic - immunology
Vaccination
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccines, DNA - administration & dosage
Vaccines, DNA - genetics
Vaccines, DNA - immunology
Virology
title Immunization with a DNA chimeric molecule encoding a hemagglutinin peptide and a scFv CD21-specific antibody fragment induces long-lasting IgM and CTL responses to influenza virus
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