Optimized DNA microarray assay allows detection and genotyping of single PCR-amplifiable target copies
This study was conducted to determine the detection limit of an optimized DNA microarray assay for detection and species identification of chlamydiae. Examination of dilution series of a plasmid standard carrying the target sequence from Chlamydia trachomatis and genomic DNA of this organism reveale...
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| Veröffentlicht in: | Molecular and cellular probes 2006-02, Vol.20 (1), p.60-63 |
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| creator | Ehricht, Ralf Slickers, Peter Goellner, Stefanie Hotzel, Helmut Sachse, Konrad |
| description | This study was conducted to determine the detection limit of an optimized DNA microarray assay for detection and species identification of chlamydiae. Examination of dilution series of a plasmid standard carrying the target sequence from
Chlamydia trachomatis and genomic DNA of this organism revealed that a single PCR-amplifiable target copy was sufficient to obtain a specific hybridization pattern. This performance renders the test suitable for routine testing of clinical samples. |
| doi_str_mv | 10.1016/j.mcp.2005.09.003 |
| format | Article |
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| ispartof | Molecular and cellular probes, 2006-02, Vol.20 (1), p.60-63 |
| issn | 0890-8508 1096-1194 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Chlamydia trachomatis Chlamydia trachomatis - genetics Detection DNA microarray Genotype Oligonucleotide Array Sequence Analysis - methods Plasmids - genetics Plasmids - metabolism Polymerase Chain Reaction Sensitivity Sensitivity and Specificity |
| title | Optimized DNA microarray assay allows detection and genotyping of single PCR-amplifiable target copies |
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