Preterm labor biomarker discovery in serum using 3 proteomic profiling methodologies
Objective The aim of this study was to identify changes in protein expression in normal pregnancy compared with preterm labor by using 3 proteomic methods. Study Design Serum was collected from 25 nonpregnant (n = 5) and pregnant women at 24–40 weeks' gestation (n = 20) who had preterm labor re...
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| Veröffentlicht in: | American journal of obstetrics and gynecology 2009-10, Vol.201 (4), p.387.e1-387.e13 |
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| creator | Stella, Caroline L., MD Bennett, Michael R., PhD Devarajan, Prasad, MD, PhD Greis, Kenneth, PhD Wyder, Michael, MS Macha, Stephen, PhD Rao, Marepalli, PhD Jodicke, Cristiano, MD Moussa, Hind, MD How, Helen Y., MD Myatt, Leslie, PhD Webster, Rose, PhD Sibai, Baha M., MD |
| description | Objective The aim of this study was to identify changes in protein expression in normal pregnancy compared with preterm labor by using 3 proteomic methods. Study Design Serum was collected from 25 nonpregnant (n = 5) and pregnant women at 24–40 weeks' gestation (n = 20) who had preterm labor resulting in preterm delivery (n = 5), preterm labor with term delivery (n = 5), term labor resulting in delivery (n = 5), or at term with contractions (n = 5). Undepleted serum was used for surface-enhanced laser desorption ionization and immune-depleted serum for matrix-assisted laser desorption ionization and 2-dimensional electrophoresis. Results Surface-enhanced laser desorption ionization identified significantly different peaks between preterm labor resulting in preterm delivery vs term labor resulting in delivery and preterm labor resulting in preterm delivery vs preterm labor with term delivery using 4 surfaces. In preterm labor resulting in preterm delivery vs preterm labor with term delivery, a peak of 7783.2 m/z was significantly up-regulated and at 3164 m/z down-regulated on 3 surfaces. By using 2-dimensional electrophoresis, protein 5364 was significantly different between preterm labor resulting in preterm delivery and term labor resulting in delivery. In preterm labor resulting in preterm delivery, 6 proteins showed decreasing trend and 1 showed increasing trend vs preterm labor with term delivery. Matrix-assisted laser desorption ionization showed a striking difference at 55,000 m/z between preterm labor resulting in preterm delivery and term labor resulting in delivery. Conclusion Surface-enhanced laser desorption ionization identified 2 proteins fulfilling the criteria of putative biomarkers. Biomarker identification may aid in identifying women with preterm labor who will deliver preterm. |
| doi_str_mv | 10.1016/j.ajog.2009.06.034 |
| format | Article |
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Study Design Serum was collected from 25 nonpregnant (n = 5) and pregnant women at 24–40 weeks' gestation (n = 20) who had preterm labor resulting in preterm delivery (n = 5), preterm labor with term delivery (n = 5), term labor resulting in delivery (n = 5), or at term with contractions (n = 5). Undepleted serum was used for surface-enhanced laser desorption ionization and immune-depleted serum for matrix-assisted laser desorption ionization and 2-dimensional electrophoresis. Results Surface-enhanced laser desorption ionization identified significantly different peaks between preterm labor resulting in preterm delivery vs term labor resulting in delivery and preterm labor resulting in preterm delivery vs preterm labor with term delivery using 4 surfaces. In preterm labor resulting in preterm delivery vs preterm labor with term delivery, a peak of 7783.2 m/z was significantly up-regulated and at 3164 m/z down-regulated on 3 surfaces. By using 2-dimensional electrophoresis, protein 5364 was significantly different between preterm labor resulting in preterm delivery and term labor resulting in delivery. In preterm labor resulting in preterm delivery, 6 proteins showed decreasing trend and 1 showed increasing trend vs preterm labor with term delivery. Matrix-assisted laser desorption ionization showed a striking difference at 55,000 m/z between preterm labor resulting in preterm delivery and term labor resulting in delivery. Conclusion Surface-enhanced laser desorption ionization identified 2 proteins fulfilling the criteria of putative biomarkers. Biomarker identification may aid in identifying women with preterm labor who will deliver preterm.</description><identifier>ISSN: 0002-9378</identifier><identifier>EISSN: 1097-6868</identifier><identifier>DOI: 10.1016/j.ajog.2009.06.034</identifier><identifier>PMID: 19716121</identifier><identifier>CODEN: AJOGAH</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>2-dimensional electrophoresis ; Analysis of Variance ; Biological and medical sciences ; Biomarkers - blood ; Diseases of mother, fetus and pregnancy ; Electrophoresis, Gel, Two-Dimensional ; Female ; Gynecology. Andrology. Obstetrics ; Humans ; MALDI ; mass spectrometry ; Medical sciences ; Obstetric Labor, Premature - diagnosis ; Obstetrics and Gynecology ; Pregnancy ; Pregnancy. Fetus. Placenta ; preterm labor ; Protein Array Analysis ; Proteomics ; SELDI ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><ispartof>American journal of obstetrics and gynecology, 2009-10, Vol.201 (4), p.387.e1-387.e13</ispartof><rights>Mosby, Inc.</rights><rights>2009 Mosby, Inc.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-c1abe37c7b9f329d78bd0bbf3bde1a3bb95cc9e91223de00c4500ac199f5b6193</citedby><cites>FETCH-LOGICAL-c439t-c1abe37c7b9f329d78bd0bbf3bde1a3bb95cc9e91223de00c4500ac199f5b6193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ajog.2009.06.034$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3550,23930,23931,25140,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22009490$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19716121$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stella, Caroline L., MD</creatorcontrib><creatorcontrib>Bennett, Michael R., PhD</creatorcontrib><creatorcontrib>Devarajan, Prasad, MD, PhD</creatorcontrib><creatorcontrib>Greis, Kenneth, PhD</creatorcontrib><creatorcontrib>Wyder, Michael, MS</creatorcontrib><creatorcontrib>Macha, Stephen, PhD</creatorcontrib><creatorcontrib>Rao, Marepalli, PhD</creatorcontrib><creatorcontrib>Jodicke, Cristiano, MD</creatorcontrib><creatorcontrib>Moussa, Hind, MD</creatorcontrib><creatorcontrib>How, Helen Y., MD</creatorcontrib><creatorcontrib>Myatt, Leslie, PhD</creatorcontrib><creatorcontrib>Webster, Rose, PhD</creatorcontrib><creatorcontrib>Sibai, Baha M., MD</creatorcontrib><title>Preterm labor biomarker discovery in serum using 3 proteomic profiling methodologies</title><title>American journal of obstetrics and gynecology</title><addtitle>Am J Obstet Gynecol</addtitle><description>Objective The aim of this study was to identify changes in protein expression in normal pregnancy compared with preterm labor by using 3 proteomic methods. Study Design Serum was collected from 25 nonpregnant (n = 5) and pregnant women at 24–40 weeks' gestation (n = 20) who had preterm labor resulting in preterm delivery (n = 5), preterm labor with term delivery (n = 5), term labor resulting in delivery (n = 5), or at term with contractions (n = 5). Undepleted serum was used for surface-enhanced laser desorption ionization and immune-depleted serum for matrix-assisted laser desorption ionization and 2-dimensional electrophoresis. Results Surface-enhanced laser desorption ionization identified significantly different peaks between preterm labor resulting in preterm delivery vs term labor resulting in delivery and preterm labor resulting in preterm delivery vs preterm labor with term delivery using 4 surfaces. In preterm labor resulting in preterm delivery vs preterm labor with term delivery, a peak of 7783.2 m/z was significantly up-regulated and at 3164 m/z down-regulated on 3 surfaces. By using 2-dimensional electrophoresis, protein 5364 was significantly different between preterm labor resulting in preterm delivery and term labor resulting in delivery. In preterm labor resulting in preterm delivery, 6 proteins showed decreasing trend and 1 showed increasing trend vs preterm labor with term delivery. Matrix-assisted laser desorption ionization showed a striking difference at 55,000 m/z between preterm labor resulting in preterm delivery and term labor resulting in delivery. Conclusion Surface-enhanced laser desorption ionization identified 2 proteins fulfilling the criteria of putative biomarkers. Biomarker identification may aid in identifying women with preterm labor who will deliver preterm.</description><subject>2-dimensional electrophoresis</subject><subject>Analysis of Variance</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - blood</subject><subject>Diseases of mother, fetus and pregnancy</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Female</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>MALDI</subject><subject>mass spectrometry</subject><subject>Medical sciences</subject><subject>Obstetric Labor, Premature - diagnosis</subject><subject>Obstetrics and Gynecology</subject><subject>Pregnancy</subject><subject>Pregnancy. Fetus. Placenta</subject><subject>preterm labor</subject><subject>Protein Array Analysis</subject><subject>Proteomics</subject><subject>SELDI</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><issn>0002-9378</issn><issn>1097-6868</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuKFDEUQIMoTjv6Ay6kNrqr8ibpTjogwjD4ggEFx3XI41abmqrKmFQN9N-b0I2CC1d5cO7rXEJeUugoUPF26MwQDx0DUB2IDvj2EdlQULIVe7F_TDYAwFrF5f6CPMt5qE-m2FNyQZWkgjK6IbffEi6YpmY0NqbGhjiZdIep8SG7-IDp2IS5yZjWqVlzmA8Nb-5TXDBOwdVbH8b6O-HyM_o4xkPA_Jw86c2Y8cX5vCQ_Pn64vf7c3nz99OX66qZ1W66W1lFjkUsnreo5U17urQdre249UsOtVTvnFCrKGPcI4LY7AOOoUv3OCqr4JXlzylv6-LViXvRUusZxNDPGNWshhZQcWAHZCXQp5pyw1_cplEGPmoKuLvWgq0tdXWoQurgsQa_O2Vc7of8bcpZXgNdnwGRnxj6Z2YX8h2M12VZB4d6dOCwuHgImnV3A2aEPCd2ifQz_7-P9P-GuKA-l4h0eMQ9xTXOxrKnOTIP-Xtdclw4KoMzP-G9QMKix</recordid><startdate>20091001</startdate><enddate>20091001</enddate><creator>Stella, Caroline L., MD</creator><creator>Bennett, Michael R., PhD</creator><creator>Devarajan, Prasad, MD, PhD</creator><creator>Greis, Kenneth, PhD</creator><creator>Wyder, Michael, MS</creator><creator>Macha, Stephen, PhD</creator><creator>Rao, Marepalli, PhD</creator><creator>Jodicke, Cristiano, MD</creator><creator>Moussa, Hind, MD</creator><creator>How, Helen Y., MD</creator><creator>Myatt, Leslie, PhD</creator><creator>Webster, Rose, PhD</creator><creator>Sibai, Baha M., MD</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20091001</creationdate><title>Preterm labor biomarker discovery in serum using 3 proteomic profiling methodologies</title><author>Stella, Caroline L., MD ; Bennett, Michael R., PhD ; Devarajan, Prasad, MD, PhD ; Greis, Kenneth, PhD ; Wyder, Michael, MS ; Macha, Stephen, PhD ; Rao, Marepalli, PhD ; Jodicke, Cristiano, MD ; Moussa, Hind, MD ; How, Helen Y., MD ; Myatt, Leslie, PhD ; Webster, Rose, PhD ; Sibai, Baha M., MD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-c1abe37c7b9f329d78bd0bbf3bde1a3bb95cc9e91223de00c4500ac199f5b6193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>2-dimensional electrophoresis</topic><topic>Analysis of Variance</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - blood</topic><topic>Diseases of mother, fetus and pregnancy</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Female</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>MALDI</topic><topic>mass spectrometry</topic><topic>Medical sciences</topic><topic>Obstetric Labor, Premature - diagnosis</topic><topic>Obstetrics and Gynecology</topic><topic>Pregnancy</topic><topic>Pregnancy. Fetus. Placenta</topic><topic>preterm labor</topic><topic>Protein Array Analysis</topic><topic>Proteomics</topic><topic>SELDI</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stella, Caroline L., MD</creatorcontrib><creatorcontrib>Bennett, Michael R., PhD</creatorcontrib><creatorcontrib>Devarajan, Prasad, MD, PhD</creatorcontrib><creatorcontrib>Greis, Kenneth, PhD</creatorcontrib><creatorcontrib>Wyder, Michael, MS</creatorcontrib><creatorcontrib>Macha, Stephen, PhD</creatorcontrib><creatorcontrib>Rao, Marepalli, PhD</creatorcontrib><creatorcontrib>Jodicke, Cristiano, MD</creatorcontrib><creatorcontrib>Moussa, Hind, MD</creatorcontrib><creatorcontrib>How, Helen Y., MD</creatorcontrib><creatorcontrib>Myatt, Leslie, PhD</creatorcontrib><creatorcontrib>Webster, Rose, PhD</creatorcontrib><creatorcontrib>Sibai, Baha M., MD</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of obstetrics and gynecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stella, Caroline L., MD</au><au>Bennett, Michael R., PhD</au><au>Devarajan, Prasad, MD, PhD</au><au>Greis, Kenneth, PhD</au><au>Wyder, Michael, MS</au><au>Macha, Stephen, PhD</au><au>Rao, Marepalli, PhD</au><au>Jodicke, Cristiano, MD</au><au>Moussa, Hind, MD</au><au>How, Helen Y., MD</au><au>Myatt, Leslie, PhD</au><au>Webster, Rose, PhD</au><au>Sibai, Baha M., MD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preterm labor biomarker discovery in serum using 3 proteomic profiling methodologies</atitle><jtitle>American journal of obstetrics and gynecology</jtitle><addtitle>Am J Obstet Gynecol</addtitle><date>2009-10-01</date><risdate>2009</risdate><volume>201</volume><issue>4</issue><spage>387.e1</spage><epage>387.e13</epage><pages>387.e1-387.e13</pages><issn>0002-9378</issn><eissn>1097-6868</eissn><coden>AJOGAH</coden><abstract>Objective The aim of this study was to identify changes in protein expression in normal pregnancy compared with preterm labor by using 3 proteomic methods. Study Design Serum was collected from 25 nonpregnant (n = 5) and pregnant women at 24–40 weeks' gestation (n = 20) who had preterm labor resulting in preterm delivery (n = 5), preterm labor with term delivery (n = 5), term labor resulting in delivery (n = 5), or at term with contractions (n = 5). Undepleted serum was used for surface-enhanced laser desorption ionization and immune-depleted serum for matrix-assisted laser desorption ionization and 2-dimensional electrophoresis. Results Surface-enhanced laser desorption ionization identified significantly different peaks between preterm labor resulting in preterm delivery vs term labor resulting in delivery and preterm labor resulting in preterm delivery vs preterm labor with term delivery using 4 surfaces. In preterm labor resulting in preterm delivery vs preterm labor with term delivery, a peak of 7783.2 m/z was significantly up-regulated and at 3164 m/z down-regulated on 3 surfaces. By using 2-dimensional electrophoresis, protein 5364 was significantly different between preterm labor resulting in preterm delivery and term labor resulting in delivery. In preterm labor resulting in preterm delivery, 6 proteins showed decreasing trend and 1 showed increasing trend vs preterm labor with term delivery. Matrix-assisted laser desorption ionization showed a striking difference at 55,000 m/z between preterm labor resulting in preterm delivery and term labor resulting in delivery. Conclusion Surface-enhanced laser desorption ionization identified 2 proteins fulfilling the criteria of putative biomarkers. Biomarker identification may aid in identifying women with preterm labor who will deliver preterm.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>19716121</pmid><doi>10.1016/j.ajog.2009.06.034</doi><tpages>3</tpages></addata></record> |
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| subjects | 2-dimensional electrophoresis Analysis of Variance Biological and medical sciences Biomarkers - blood Diseases of mother, fetus and pregnancy Electrophoresis, Gel, Two-Dimensional Female Gynecology. Andrology. Obstetrics Humans MALDI mass spectrometry Medical sciences Obstetric Labor, Premature - diagnosis Obstetrics and Gynecology Pregnancy Pregnancy. Fetus. Placenta preterm labor Protein Array Analysis Proteomics SELDI Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| title | Preterm labor biomarker discovery in serum using 3 proteomic profiling methodologies |
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