Micromachined sensor for lactate monitoring in saliva
A miniaturised sensor for continuous lactate measurement in saliva was developed and tested. The sensor was built using silicon microfabrication technologies. The size of the chip is 5.5 mm × 6.4 mm × 0.7 mm and features a working, a counter and an Iridium reference electrode. The chip has a cavity...
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| Veröffentlicht in: | Biosensors & bioelectronics 2006-03, Vol.21 (9), p.1770-1776 |
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| container_title | Biosensors & bioelectronics |
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| creator | Schabmueller, C.G.J. Loppow, D. Piechotta, G. Schütze, B. Albers, J. Hintsche, R. |
| description | A miniaturised sensor for continuous lactate measurement in saliva was developed and tested. The sensor was built using silicon microfabrication technologies. The size of the chip is 5.5
mm
×
6.4
mm
×
0.7
mm and features a working, a counter and an Iridium reference electrode. The chip has a cavity whose floor is perforated by fine pores. The cavity contains the enzyme lactate oxidase (LOD), which is immobilised in an agarose gel. Prior to the amperometric detection of the reaction product hydrogen peroxide at the working electrode, the analyte lactate has to pass the pores to reach the cavity with the lactate oxidase by diffusion.
To test the silicon sensor, capillary blood and saliva samples were obtained during standardised ergometer tests. Salivary lactate concentrations were determined with the sensor and compared to photometrically derived data from a lab-automate. In addition the saliva data were compared to standard capillary blood lactate concentrations measured with a pocket photometer. Lactate concentration versus load graphs were plotted and compared visually showing very similar progressions. The novel approach enables a location independent, permanent real-time measurement of the lactate concentration during exercise. |
| doi_str_mv | 10.1016/j.bios.2005.09.015 |
| format | Article |
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mm
×
6.4
mm
×
0.7
mm and features a working, a counter and an Iridium reference electrode. The chip has a cavity whose floor is perforated by fine pores. The cavity contains the enzyme lactate oxidase (LOD), which is immobilised in an agarose gel. Prior to the amperometric detection of the reaction product hydrogen peroxide at the working electrode, the analyte lactate has to pass the pores to reach the cavity with the lactate oxidase by diffusion.
To test the silicon sensor, capillary blood and saliva samples were obtained during standardised ergometer tests. Salivary lactate concentrations were determined with the sensor and compared to photometrically derived data from a lab-automate. In addition the saliva data were compared to standard capillary blood lactate concentrations measured with a pocket photometer. Lactate concentration versus load graphs were plotted and compared visually showing very similar progressions. The novel approach enables a location independent, permanent real-time measurement of the lactate concentration during exercise.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2005.09.015</identifier><identifier>PMID: 16289607</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Amperometric measurement ; Biological and medical sciences ; Biosensing Techniques - instrumentation ; Biosensing Techniques - methods ; Biosensors ; Biotechnology ; Electrochemistry - instrumentation ; Electrochemistry - methods ; Equipment Design ; Equipment Failure Analysis ; Fundamental and applied biological sciences. Psychology ; Humans ; Iridium reference electrode ; Lactate sensor ; Lactic Acid - analysis ; Lactic Acid - blood ; Methods. Procedures. Technologies ; Miniaturization ; Reproducibility of Results ; Saliva ; Saliva - chemistry ; Sensitivity and Specificity ; Silicon enzyme sensor ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 2006-03, Vol.21 (9), p.1770-1776</ispartof><rights>2005 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-c333f6a095375e824c38eb6f0eef71ea3157b9b09bf895b1241265182cf281f03</citedby><cites>FETCH-LOGICAL-c415t-c333f6a095375e824c38eb6f0eef71ea3157b9b09bf895b1241265182cf281f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2005.09.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17562883$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16289607$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schabmueller, C.G.J.</creatorcontrib><creatorcontrib>Loppow, D.</creatorcontrib><creatorcontrib>Piechotta, G.</creatorcontrib><creatorcontrib>Schütze, B.</creatorcontrib><creatorcontrib>Albers, J.</creatorcontrib><creatorcontrib>Hintsche, R.</creatorcontrib><title>Micromachined sensor for lactate monitoring in saliva</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>A miniaturised sensor for continuous lactate measurement in saliva was developed and tested. The sensor was built using silicon microfabrication technologies. The size of the chip is 5.5
mm
×
6.4
mm
×
0.7
mm and features a working, a counter and an Iridium reference electrode. The chip has a cavity whose floor is perforated by fine pores. The cavity contains the enzyme lactate oxidase (LOD), which is immobilised in an agarose gel. Prior to the amperometric detection of the reaction product hydrogen peroxide at the working electrode, the analyte lactate has to pass the pores to reach the cavity with the lactate oxidase by diffusion.
To test the silicon sensor, capillary blood and saliva samples were obtained during standardised ergometer tests. Salivary lactate concentrations were determined with the sensor and compared to photometrically derived data from a lab-automate. In addition the saliva data were compared to standard capillary blood lactate concentrations measured with a pocket photometer. Lactate concentration versus load graphs were plotted and compared visually showing very similar progressions. The novel approach enables a location independent, permanent real-time measurement of the lactate concentration during exercise.</description><subject>Amperometric measurement</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Electrochemistry - instrumentation</subject><subject>Electrochemistry - methods</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Iridium reference electrode</subject><subject>Lactate sensor</subject><subject>Lactic Acid - analysis</subject><subject>Lactic Acid - blood</subject><subject>Methods. Procedures. Technologies</subject><subject>Miniaturization</subject><subject>Reproducibility of Results</subject><subject>Saliva</subject><subject>Saliva - chemistry</subject><subject>Sensitivity and Specificity</subject><subject>Silicon enzyme sensor</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKBDEQRYMoOj5-wIX0RnfdVpJO0gE3Ir5AcaPrkM5UNEM_NOkR_HszzMDslKKozblF1SHklEJFgcrLRdWGMVUMQFSgK6Bih8xoo3hZMy52yQy0kKWQkh-Qw5QWAKCohn1yQCVrtAQ1I-I5uDj21n2EAedFwiGNsfC5O-smO2HRj0OYxhiG9yIMRbJd-LbHZM_bLuHJZh6Rt7vb15uH8unl_vHm-ql0NRVT6TjnXtp8BlcCG1Y73mArPSB6RdFyKlSrW9Ctb7RoKaspk4I2zHnWUA_8iFys937G8WuJaTJ9SA67zg44LpORalWM_QtSXTMGSmWQrcH8dUoRvfmMobfxx1AwK6tmYVZWzcqqAW2y1Rw622xftj3Ot5GNxgycbwCbnO18tIMLacspkcmGZ-5qzWGW9h0wmuQCDg7nIaKbzHwMf93xC_VjlCg</recordid><startdate>20060315</startdate><enddate>20060315</enddate><creator>Schabmueller, C.G.J.</creator><creator>Loppow, D.</creator><creator>Piechotta, G.</creator><creator>Schütze, B.</creator><creator>Albers, J.</creator><creator>Hintsche, R.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060315</creationdate><title>Micromachined sensor for lactate monitoring in saliva</title><author>Schabmueller, C.G.J. ; Loppow, D. ; Piechotta, G. ; Schütze, B. ; Albers, J. ; Hintsche, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-c333f6a095375e824c38eb6f0eef71ea3157b9b09bf895b1241265182cf281f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amperometric measurement</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Electrochemistry - instrumentation</topic><topic>Electrochemistry - methods</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Iridium reference electrode</topic><topic>Lactate sensor</topic><topic>Lactic Acid - analysis</topic><topic>Lactic Acid - blood</topic><topic>Methods. Procedures. Technologies</topic><topic>Miniaturization</topic><topic>Reproducibility of Results</topic><topic>Saliva</topic><topic>Saliva - chemistry</topic><topic>Sensitivity and Specificity</topic><topic>Silicon enzyme sensor</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schabmueller, C.G.J.</creatorcontrib><creatorcontrib>Loppow, D.</creatorcontrib><creatorcontrib>Piechotta, G.</creatorcontrib><creatorcontrib>Schütze, B.</creatorcontrib><creatorcontrib>Albers, J.</creatorcontrib><creatorcontrib>Hintsche, R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schabmueller, C.G.J.</au><au>Loppow, D.</au><au>Piechotta, G.</au><au>Schütze, B.</au><au>Albers, J.</au><au>Hintsche, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Micromachined sensor for lactate monitoring in saliva</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2006-03-15</date><risdate>2006</risdate><volume>21</volume><issue>9</issue><spage>1770</spage><epage>1776</epage><pages>1770-1776</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>A miniaturised sensor for continuous lactate measurement in saliva was developed and tested. The sensor was built using silicon microfabrication technologies. The size of the chip is 5.5
mm
×
6.4
mm
×
0.7
mm and features a working, a counter and an Iridium reference electrode. The chip has a cavity whose floor is perforated by fine pores. The cavity contains the enzyme lactate oxidase (LOD), which is immobilised in an agarose gel. Prior to the amperometric detection of the reaction product hydrogen peroxide at the working electrode, the analyte lactate has to pass the pores to reach the cavity with the lactate oxidase by diffusion.
To test the silicon sensor, capillary blood and saliva samples were obtained during standardised ergometer tests. Salivary lactate concentrations were determined with the sensor and compared to photometrically derived data from a lab-automate. In addition the saliva data were compared to standard capillary blood lactate concentrations measured with a pocket photometer. Lactate concentration versus load graphs were plotted and compared visually showing very similar progressions. The novel approach enables a location independent, permanent real-time measurement of the lactate concentration during exercise.</abstract><cop>Lausanne</cop><pub>Elsevier B.V</pub><pmid>16289607</pmid><doi>10.1016/j.bios.2005.09.015</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biosensors & bioelectronics, 2006-03, Vol.21 (9), p.1770-1776 |
| issn | 0956-5663 1873-4235 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amperometric measurement Biological and medical sciences Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensors Biotechnology Electrochemistry - instrumentation Electrochemistry - methods Equipment Design Equipment Failure Analysis Fundamental and applied biological sciences. Psychology Humans Iridium reference electrode Lactate sensor Lactic Acid - analysis Lactic Acid - blood Methods. Procedures. Technologies Miniaturization Reproducibility of Results Saliva Saliva - chemistry Sensitivity and Specificity Silicon enzyme sensor Various methods and equipments |
| title | Micromachined sensor for lactate monitoring in saliva |
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