Electrosynthesized poly(pyrrole)/poly(2-naphthol) bilayer membrane as an effective anti-interference layer for simultaneous determination of acethylcholine and choline by a dual electrode amperometric biosensor
Several neural diseases appear related to the neurotransmitter acethylcholine (ACh) and its metabolite choline (Ch) brain levels so that their simultaneous determination is essential. A cross-talk and interference free dual electrode amperometric biosensor for the simultaneous determination of both...
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| Veröffentlicht in: | Biosensors & bioelectronics 2006-03, Vol.21 (9), p.1710-1718 |
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| creator | Guerrieri, A. Lattanzio, V. Palmisano, F. Zambonin, P.G. |
| description | Several neural diseases appear related to the neurotransmitter acethylcholine (ACh) and its metabolite choline (Ch) brain levels so that their simultaneous determination is essential. A cross-talk and interference free dual electrode amperometric biosensor for the simultaneous determination of both analytes has been developed. Acetylcholinesterase (AChE) and choline oxidase (ChO) were immobilized by glutaraldehyde co-crosslinking with bovine serum albumin. A very efficient rejection of electroactive interferents has been achieved by a novel electrosynthesized polymeric bilayer membrane composed by overoxidised poly(pyrrole) and poly(2-naphthol) films. Sensitivities towards several electroactive interferents ranged from ca. 0.04% (e.g. ascorbate) to ca. 0.3% (e.g. dopamine) of those relevant to ACh and Ch (11 and 15
μA/μM, respectively). Detection limits (at S/N
=
3) in flow injection analysis were ca. 100
nM for both ACh and Ch at the ChO–AChE electrode and ca. 40
nM for Ch at the ChO sensor. Biosensor performances appear more than adequate for brain tissue homogenates and cerebrospinal fluids analysis where average levels in the low micromolar range are typically found. |
| doi_str_mv | 10.1016/j.bios.2005.08.005 |
| format | Article |
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μA/μM, respectively). Detection limits (at S/N
=
3) in flow injection analysis were ca. 100
nM for both ACh and Ch at the ChO–AChE electrode and ca. 40
nM for Ch at the ChO sensor. Biosensor performances appear more than adequate for brain tissue homogenates and cerebrospinal fluids analysis where average levels in the low micromolar range are typically found.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2005.08.005</identifier><identifier>PMID: 16169212</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Acetylcholine - analysis ; Acetylcholine - chemistry ; Acetylcholinesterase - analysis ; Acetylcholinesterase - chemistry ; Alcohol Oxidoreductases - analysis ; Alcohol Oxidoreductases - chemistry ; Biological and medical sciences ; Biosensing Techniques - instrumentation ; Biosensing Techniques - methods ; Biosensor ; Biosensors ; Biotechnology ; Choline - analysis ; Choline - chemistry ; Dual electrode sensor ; Electrochemistry - instrumentation ; Electrochemistry - methods ; Enzyme immobilization ; Equipment Design ; Equipment Failure Analysis ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. Technologies ; Microelectrodes ; Naphthols - chemistry ; Non-conducting polymers ; Polymers - chemistry ; Pyrroles - chemistry ; Reproducibility of Results ; Sensitivity and Specificity ; Simultaneous determination ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 2006-03, Vol.21 (9), p.1710-1718</ispartof><rights>2005 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-223a79a7fcea2972914bb97f577829f86fddc68a76da7cbeb02e9873b0970aab3</citedby><cites>FETCH-LOGICAL-c384t-223a79a7fcea2972914bb97f577829f86fddc68a76da7cbeb02e9873b0970aab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0956566305002502$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17562876$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16169212$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guerrieri, A.</creatorcontrib><creatorcontrib>Lattanzio, V.</creatorcontrib><creatorcontrib>Palmisano, F.</creatorcontrib><creatorcontrib>Zambonin, P.G.</creatorcontrib><title>Electrosynthesized poly(pyrrole)/poly(2-naphthol) bilayer membrane as an effective anti-interference layer for simultaneous determination of acethylcholine and choline by a dual electrode amperometric biosensor</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>Several neural diseases appear related to the neurotransmitter acethylcholine (ACh) and its metabolite choline (Ch) brain levels so that their simultaneous determination is essential. A cross-talk and interference free dual electrode amperometric biosensor for the simultaneous determination of both analytes has been developed. Acetylcholinesterase (AChE) and choline oxidase (ChO) were immobilized by glutaraldehyde co-crosslinking with bovine serum albumin. A very efficient rejection of electroactive interferents has been achieved by a novel electrosynthesized polymeric bilayer membrane composed by overoxidised poly(pyrrole) and poly(2-naphthol) films. Sensitivities towards several electroactive interferents ranged from ca. 0.04% (e.g. ascorbate) to ca. 0.3% (e.g. dopamine) of those relevant to ACh and Ch (11 and 15
μA/μM, respectively). Detection limits (at S/N
=
3) in flow injection analysis were ca. 100
nM for both ACh and Ch at the ChO–AChE electrode and ca. 40
nM for Ch at the ChO sensor. Biosensor performances appear more than adequate for brain tissue homogenates and cerebrospinal fluids analysis where average levels in the low micromolar range are typically found.</description><subject>Acetylcholine - analysis</subject><subject>Acetylcholine - chemistry</subject><subject>Acetylcholinesterase - analysis</subject><subject>Acetylcholinesterase - chemistry</subject><subject>Alcohol Oxidoreductases - analysis</subject><subject>Alcohol Oxidoreductases - chemistry</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensor</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Choline - analysis</subject><subject>Choline - chemistry</subject><subject>Dual electrode sensor</subject><subject>Electrochemistry - instrumentation</subject><subject>Electrochemistry - methods</subject><subject>Enzyme immobilization</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. Technologies</subject><subject>Microelectrodes</subject><subject>Naphthols - chemistry</subject><subject>Non-conducting polymers</subject><subject>Polymers - chemistry</subject><subject>Pyrroles - chemistry</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Simultaneous determination</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1DAUhSMEokPhBVggb0DtIqnjTOxEYoOq8iNVYgNryz_XGo8cO9ieSuExeSKcZlB3yItry989vj6nqt62uGlxS2-OjbQhNQTjvsFDU8qzatcOrKv3pOufVzs89rTuKe0uqlcpHTHGrB3xy-qipS0dSUt21Z87ByrHkBafD5Dsb9BoDm65mpcYg4Prm8cTqb2YD_kQ3DWS1okFIppgklF4QCIh4REYU5TsQzn7bGvrM0QDEbwCtDWYEFGy08nl0hVOCWkozGS9yDZ4FAwSCvJhcaq8Y1dhr9G_vVyQQPokHIJtYl3upxlimCBHq9DqBfgU4uvqhREuwZtzvax-fr77cfu1vv_-5dvtp_tadcM-14R0go2CGQWCjIyM7V7KkZmesYGMZqBGa0UHwagWTEmQmMBYzJV4ZFgI2V1WHzbdOYZfJ0iZTzYpcG77HaesLEpxAckGquJzimD4HO0k4sJbzNck-ZGv0_M1SY4HXkppendWP8kJ9FPLOboCvD8DIinhTIlC2fTEsZ6SgdHCfdw4KF48WIg8Kbumom0sRnId7P_m-At7a8Po</recordid><startdate>20060315</startdate><enddate>20060315</enddate><creator>Guerrieri, A.</creator><creator>Lattanzio, V.</creator><creator>Palmisano, F.</creator><creator>Zambonin, P.G.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060315</creationdate><title>Electrosynthesized poly(pyrrole)/poly(2-naphthol) bilayer membrane as an effective anti-interference layer for simultaneous determination of acethylcholine and choline by a dual electrode amperometric biosensor</title><author>Guerrieri, A. ; Lattanzio, V. ; Palmisano, F. ; Zambonin, P.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-223a79a7fcea2972914bb97f577829f86fddc68a76da7cbeb02e9873b0970aab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Acetylcholine - analysis</topic><topic>Acetylcholine - chemistry</topic><topic>Acetylcholinesterase - analysis</topic><topic>Acetylcholinesterase - chemistry</topic><topic>Alcohol Oxidoreductases - analysis</topic><topic>Alcohol Oxidoreductases - chemistry</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensor</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Choline - analysis</topic><topic>Choline - chemistry</topic><topic>Dual electrode sensor</topic><topic>Electrochemistry - instrumentation</topic><topic>Electrochemistry - methods</topic><topic>Enzyme immobilization</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Methods. Procedures. Technologies</topic><topic>Microelectrodes</topic><topic>Naphthols - chemistry</topic><topic>Non-conducting polymers</topic><topic>Polymers - chemistry</topic><topic>Pyrroles - chemistry</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Simultaneous determination</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guerrieri, A.</creatorcontrib><creatorcontrib>Lattanzio, V.</creatorcontrib><creatorcontrib>Palmisano, F.</creatorcontrib><creatorcontrib>Zambonin, P.G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guerrieri, A.</au><au>Lattanzio, V.</au><au>Palmisano, F.</au><au>Zambonin, P.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrosynthesized poly(pyrrole)/poly(2-naphthol) bilayer membrane as an effective anti-interference layer for simultaneous determination of acethylcholine and choline by a dual electrode amperometric biosensor</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2006-03-15</date><risdate>2006</risdate><volume>21</volume><issue>9</issue><spage>1710</spage><epage>1718</epage><pages>1710-1718</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>Several neural diseases appear related to the neurotransmitter acethylcholine (ACh) and its metabolite choline (Ch) brain levels so that their simultaneous determination is essential. A cross-talk and interference free dual electrode amperometric biosensor for the simultaneous determination of both analytes has been developed. Acetylcholinesterase (AChE) and choline oxidase (ChO) were immobilized by glutaraldehyde co-crosslinking with bovine serum albumin. A very efficient rejection of electroactive interferents has been achieved by a novel electrosynthesized polymeric bilayer membrane composed by overoxidised poly(pyrrole) and poly(2-naphthol) films. Sensitivities towards several electroactive interferents ranged from ca. 0.04% (e.g. ascorbate) to ca. 0.3% (e.g. dopamine) of those relevant to ACh and Ch (11 and 15
μA/μM, respectively). Detection limits (at S/N
=
3) in flow injection analysis were ca. 100
nM for both ACh and Ch at the ChO–AChE electrode and ca. 40
nM for Ch at the ChO sensor. Biosensor performances appear more than adequate for brain tissue homogenates and cerebrospinal fluids analysis where average levels in the low micromolar range are typically found.</abstract><cop>Lausanne</cop><pub>Elsevier B.V</pub><pmid>16169212</pmid><doi>10.1016/j.bios.2005.08.005</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0956-5663 |
| ispartof | Biosensors & bioelectronics, 2006-03, Vol.21 (9), p.1710-1718 |
| issn | 0956-5663 1873-4235 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acetylcholine - analysis Acetylcholine - chemistry Acetylcholinesterase - analysis Acetylcholinesterase - chemistry Alcohol Oxidoreductases - analysis Alcohol Oxidoreductases - chemistry Biological and medical sciences Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensor Biosensors Biotechnology Choline - analysis Choline - chemistry Dual electrode sensor Electrochemistry - instrumentation Electrochemistry - methods Enzyme immobilization Equipment Design Equipment Failure Analysis Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Microelectrodes Naphthols - chemistry Non-conducting polymers Polymers - chemistry Pyrroles - chemistry Reproducibility of Results Sensitivity and Specificity Simultaneous determination Various methods and equipments |
| title | Electrosynthesized poly(pyrrole)/poly(2-naphthol) bilayer membrane as an effective anti-interference layer for simultaneous determination of acethylcholine and choline by a dual electrode amperometric biosensor |
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