A reassessment of semiquantitative analytical procedures for DNA methylation: Comparison of bisulfite- and HpaII polymerase-chain-reaction-based methods

Two techniques in particular are used to study site-specific DNA methylation: genomic sequencing after bisulfite modification and polymerase chain reaction after digestion by a methylation-sensitive endonuclease (usually HpaII). Only the former methodology assesses the methylation status of all the...

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Veröffentlicht in:	Analytical biochemistry 2006-03, Vol.350 (1), p.24-31
Hauptverfasser:	Fuso, Andrea, Scarpa, Sigfrido, Grandoni, Francesco, Strom, Roberto, Lucarelli, Marco
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bisulfite modification Cell Line DNA - chemistry DNA Methylation DNA-Cytosine Methylases - metabolism HpaII/PCR Methylation-sensitive PCR Mice Myogenin - genetics Nucleic Acid Denaturation Polymerase Chain Reaction - methods Sulfites - chemistry
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