Differences in the Activation of Inhibitory Motoneuron Receptors in the Frog Rana Ridibunda by GABA and Glycine and Their Interaction
Intracellular recording of potentials was used in isolated spinal cord segments from the frog Rana ridibunda to compare the inhibitory effects of GABA and glycine on the motoneuron membrane. At equal concentrations, the response (a change in membrane potential) to application of glycine was 1.5–2 ti...
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| Veröffentlicht in: | Neuroscience and behavioral physiology 2009-10, Vol.39 (8), p.775-783 |
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| creator | Kalinina, N. I. Kurchavyi, G. G. Amakhin, D. V. Veselkin, N. P. |
| description | Intracellular recording of potentials was used in isolated spinal cord segments from the frog
Rana ridibunda
to compare the inhibitory effects of GABA and glycine on the motoneuron membrane. At equal concentrations, the response (a change in membrane potential) to application of glycine was 1.5–2 times greater than the response to GABA in terms of amplitude, and EC
50
values were 0.75 and 1.57 mM, respectively. The response to simultaneous application of GABA and glycine averaged 79.1 ± 2.4% (
n
= 19) of the sum of the individual responses and 130.1 ± 1.5% (
n
= 19) of the glycine response (partial occlusion). Preliminary application of glycine decreased the GABA response by 85.3 ± 0.2% (
n
= 10), while preapplication of GABA decreased the glycine response by only 52.9 ± 0.3% (
n
= 11). The glycine and GABA responses were specifically suppressed by strychnine and bicuculline. These results provide evidence that as in mammals, amphibian motoneurons have both glycine (predominantly) and GABA
A
receptors; they also show that asymmetrical cross inhibition can occur. |
| doi_str_mv | 10.1007/s11055-009-9192-9 |
| format | Article |
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Rana ridibunda
to compare the inhibitory effects of GABA and glycine on the motoneuron membrane. At equal concentrations, the response (a change in membrane potential) to application of glycine was 1.5–2 times greater than the response to GABA in terms of amplitude, and EC
50
values were 0.75 and 1.57 mM, respectively. The response to simultaneous application of GABA and glycine averaged 79.1 ± 2.4% (
n
= 19) of the sum of the individual responses and 130.1 ± 1.5% (
n
= 19) of the glycine response (partial occlusion). Preliminary application of glycine decreased the GABA response by 85.3 ± 0.2% (
n
= 10), while preapplication of GABA decreased the glycine response by only 52.9 ± 0.3% (
n
= 11). The glycine and GABA responses were specifically suppressed by strychnine and bicuculline. These results provide evidence that as in mammals, amphibian motoneurons have both glycine (predominantly) and GABA
A
receptors; they also show that asymmetrical cross inhibition can occur.</description><identifier>ISSN: 0097-0549</identifier><identifier>EISSN: 1573-899X</identifier><identifier>DOI: 10.1007/s11055-009-9192-9</identifier><identifier>PMID: 19779830</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Animals ; Behavioral Sciences ; Biomedical and Life Sciences ; Biomedicine ; GABA-A Receptor Agonists ; gamma-Aminobutyric Acid - pharmacology ; Glycine - pharmacology ; In Vitro Techniques ; Membrane Potentials - drug effects ; Motor Neurons - drug effects ; Motor Neurons - physiology ; Neurobiology ; Neurosciences ; Rana ridibunda ; Receptors, GABA-A - physiology ; Receptors, Glycine - agonists ; Receptors, Glycine - physiology ; Spinal Cord - cytology</subject><ispartof>Neuroscience and behavioral physiology, 2009-10, Vol.39 (8), p.775-783</ispartof><rights>Springer Science+Business Media, Inc. 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3159-2eb6790c05111e0cebdc266895d2a7e28e1e35f83459f2609f94458e3839b0293</citedby><cites>FETCH-LOGICAL-c3159-2eb6790c05111e0cebdc266895d2a7e28e1e35f83459f2609f94458e3839b0293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11055-009-9192-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11055-009-9192-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19779830$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kalinina, N. I.</creatorcontrib><creatorcontrib>Kurchavyi, G. G.</creatorcontrib><creatorcontrib>Amakhin, D. V.</creatorcontrib><creatorcontrib>Veselkin, N. P.</creatorcontrib><title>Differences in the Activation of Inhibitory Motoneuron Receptors in the Frog Rana Ridibunda by GABA and Glycine and Their Interaction</title><title>Neuroscience and behavioral physiology</title><addtitle>Neurosci Behav Physi</addtitle><addtitle>Neurosci Behav Physiol</addtitle><description>Intracellular recording of potentials was used in isolated spinal cord segments from the frog
Rana ridibunda
to compare the inhibitory effects of GABA and glycine on the motoneuron membrane. At equal concentrations, the response (a change in membrane potential) to application of glycine was 1.5–2 times greater than the response to GABA in terms of amplitude, and EC
50
values were 0.75 and 1.57 mM, respectively. The response to simultaneous application of GABA and glycine averaged 79.1 ± 2.4% (
n
= 19) of the sum of the individual responses and 130.1 ± 1.5% (
n
= 19) of the glycine response (partial occlusion). Preliminary application of glycine decreased the GABA response by 85.3 ± 0.2% (
n
= 10), while preapplication of GABA decreased the glycine response by only 52.9 ± 0.3% (
n
= 11). The glycine and GABA responses were specifically suppressed by strychnine and bicuculline. These results provide evidence that as in mammals, amphibian motoneurons have both glycine (predominantly) and GABA
A
receptors; they also show that asymmetrical cross inhibition can occur.</description><subject>Animals</subject><subject>Behavioral Sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>GABA-A Receptor Agonists</subject><subject>gamma-Aminobutyric Acid - pharmacology</subject><subject>Glycine - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Membrane Potentials - drug effects</subject><subject>Motor Neurons - drug effects</subject><subject>Motor Neurons - physiology</subject><subject>Neurobiology</subject><subject>Neurosciences</subject><subject>Rana ridibunda</subject><subject>Receptors, GABA-A - physiology</subject><subject>Receptors, Glycine - agonists</subject><subject>Receptors, Glycine - physiology</subject><subject>Spinal Cord - cytology</subject><issn>0097-0549</issn><issn>1573-899X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkV-LEzEUxYMobl39AL5I8MG30ZtM8--xrrt1YUUoK_gWMpk72yxtUpMZoR_A772pLS4I4lNCzu-ce8Mh5DWD9wxAfSiMgRANgGkMM7wxT8iMCdU22pjvT8msCqoBMTdn5EUp91A9SsNzcsaMUka3MCO_PoVhwIzRY6Eh0nGNdOHH8NONIUWaBnod16ELY8p7-iWNKeKUq7BCj7v6-Md0ldMdXbno6Cr0oZti72i3p8vFxwV1safLzd6HiL_vt2sMuQaPmJ0_zHlJng1uU_DV6Twn364uby8-Nzdfl9cXi5vGt0yYhmMnlQEPgjGG4LHrPZdSG9Fzp5BrZNiKQbdzYQYuwQxmPhcaW92aDrhpz8m7Y-4upx8TltFuQ_G42biIaSpWKillC_K_IGcgtVSsgm__Au_TlGP9hOWc10UkQIXYEfI5lZJxsLscti7vLQN7aNIem7S1MHto0h5WfXMKnrot9o-OU3UV4EegVCneYX6c_O_UBw60p9c</recordid><startdate>200910</startdate><enddate>200910</enddate><creator>Kalinina, N. 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I.</au><au>Kurchavyi, G. G.</au><au>Amakhin, D. V.</au><au>Veselkin, N. P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differences in the Activation of Inhibitory Motoneuron Receptors in the Frog Rana Ridibunda by GABA and Glycine and Their Interaction</atitle><jtitle>Neuroscience and behavioral physiology</jtitle><stitle>Neurosci Behav Physi</stitle><addtitle>Neurosci Behav Physiol</addtitle><date>2009-10</date><risdate>2009</risdate><volume>39</volume><issue>8</issue><spage>775</spage><epage>783</epage><pages>775-783</pages><issn>0097-0549</issn><eissn>1573-899X</eissn><abstract>Intracellular recording of potentials was used in isolated spinal cord segments from the frog
Rana ridibunda
to compare the inhibitory effects of GABA and glycine on the motoneuron membrane. At equal concentrations, the response (a change in membrane potential) to application of glycine was 1.5–2 times greater than the response to GABA in terms of amplitude, and EC
50
values were 0.75 and 1.57 mM, respectively. The response to simultaneous application of GABA and glycine averaged 79.1 ± 2.4% (
n
= 19) of the sum of the individual responses and 130.1 ± 1.5% (
n
= 19) of the glycine response (partial occlusion). Preliminary application of glycine decreased the GABA response by 85.3 ± 0.2% (
n
= 10), while preapplication of GABA decreased the glycine response by only 52.9 ± 0.3% (
n
= 11). The glycine and GABA responses were specifically suppressed by strychnine and bicuculline. These results provide evidence that as in mammals, amphibian motoneurons have both glycine (predominantly) and GABA
A
receptors; they also show that asymmetrical cross inhibition can occur.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>19779830</pmid><doi>10.1007/s11055-009-9192-9</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0097-0549 |
| ispartof | Neuroscience and behavioral physiology, 2009-10, Vol.39 (8), p.775-783 |
| issn | 0097-0549 1573-899X |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Animals Behavioral Sciences Biomedical and Life Sciences Biomedicine GABA-A Receptor Agonists gamma-Aminobutyric Acid - pharmacology Glycine - pharmacology In Vitro Techniques Membrane Potentials - drug effects Motor Neurons - drug effects Motor Neurons - physiology Neurobiology Neurosciences Rana ridibunda Receptors, GABA-A - physiology Receptors, Glycine - agonists Receptors, Glycine - physiology Spinal Cord - cytology |
| title | Differences in the Activation of Inhibitory Motoneuron Receptors in the Frog Rana Ridibunda by GABA and Glycine and Their Interaction |
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