Restricted κ/λ light chain ratio by flow cytometry in germinal center B cells in hashimoto thyroiditis
To determine the diagnostic significance of the kappa/lambda ratio in germinal center (GC) B cells in Hashimoto thyroiditis (HT), we used 4-color flow cytometry to immunophenotype 27 samples (21 patients) of well-characterized HT B-cell clonality was analyzed further by polymerase chain reaction (PC...
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| Veröffentlicht in: | American journal of clinical pathology 2006, Vol.125 (1), p.42-48 |
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| creator | CHEN, Henry I AKPOLAT, Ikser MODY, Dina R LOPEZ-TERRADA, Dolores DE LEON, Audrey Ponce YAMIN LUO JORGENSEN, Jeffrey SCHWARTZ, Mary R CHANG, Chung-Che |
| description | To determine the diagnostic significance of the kappa/lambda ratio in germinal center (GC) B cells in Hashimoto thyroiditis (HT), we used 4-color flow cytometry to immunophenotype 27 samples (21 patients) of well-characterized HT B-cell clonality was analyzed further by polymerase chain reaction (PCR) of the immunoglobulin heavy chain (IgH) and bcl-2/IgH fusion genes using DNA extracted from aspirate smears and/or paraffin-embedded tissues. By flow cytometric analysis, the CD10+ GC B cells had a higher mean +/- SD kappa/lambda ratio than the CD10- B cells (5.1 +/- 3.3 vs 2.0 +/- 0.8; P < .0001, Student t test). In 18 samples (67%), CD10+ GC B cells had a kappa/lambda ratio greater than 3.07 (the upper limit of kappa/lambda ratio reported in reactive nodes; range, 3.2-14.4 in the 18 cases). Cases tested by PCR showed no evidence of a clonal proliferation. None of 21 cases developed lymphoma during clinical follow-up of up to 3 years. The kappa/lambda ratio of CD10+ GC B cells in HT can be skewed markedly beyond that reported in reactive lymph nodes. This finding frequently is present in HT. Pathologists should be familiar with this phenomenon to prevent misdiagnosis of follicular lymphoma in patients with HT. |
| doi_str_mv | 10.1309/1G5F902YE7DDUNMA |
| format | Article |
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By flow cytometric analysis, the CD10+ GC B cells had a higher mean +/- SD kappa/lambda ratio than the CD10- B cells (5.1 +/- 3.3 vs 2.0 +/- 0.8; P < .0001, Student t test). In 18 samples (67%), CD10+ GC B cells had a kappa/lambda ratio greater than 3.07 (the upper limit of kappa/lambda ratio reported in reactive nodes; range, 3.2-14.4 in the 18 cases). Cases tested by PCR showed no evidence of a clonal proliferation. None of 21 cases developed lymphoma during clinical follow-up of up to 3 years. The kappa/lambda ratio of CD10+ GC B cells in HT can be skewed markedly beyond that reported in reactive lymph nodes. This finding frequently is present in HT. Pathologists should be familiar with this phenomenon to prevent misdiagnosis of follicular lymphoma in patients with HT.</description><identifier>ISSN: 0002-9173</identifier><identifier>EISSN: 1943-7722</identifier><identifier>DOI: 10.1309/1G5F902YE7DDUNMA</identifier><identifier>PMID: 16482990</identifier><identifier>CODEN: AJCPAI</identifier><language>eng</language><publisher>Chicago, IL: American Society of Clinical Pathologists</publisher><subject>Adult ; Aged ; B-Lymphocytes - immunology ; Biological and medical sciences ; Clone Cells - immunology ; Endocrinopathies ; Female ; Flow Cytometry - methods ; Gene Rearrangement, B-Lymphocyte, Heavy Chain ; Germinal Center - cytology ; Hashimoto Disease - immunology ; Hashimoto Disease - pathology ; Humans ; Immunoglobulin kappa-Chains - analysis ; Immunoglobulin lambda-Chains - analysis ; Investigative techniques, diagnostic techniques (general aspects) ; Medical sciences ; Middle Aged ; Neprilysin - analysis ; Non tumoral diseases. Target tissue resistance. Benign neoplasms ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Polymerase Chain Reaction ; Retrospective Studies ; Thyroid Gland - immunology ; Thyroid Gland - pathology ; Thyroid. Thyroid axis (diseases)</subject><ispartof>American journal of clinical pathology, 2006, Vol.125 (1), p.42-48</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c369t-a0e13584b82ef1f1947c9cacce49d160bc0d15566b01515f7beecbbdf9d6fd323</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17477534$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16482990$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CHEN, Henry I</creatorcontrib><creatorcontrib>AKPOLAT, Ikser</creatorcontrib><creatorcontrib>MODY, Dina R</creatorcontrib><creatorcontrib>LOPEZ-TERRADA, Dolores</creatorcontrib><creatorcontrib>DE LEON, Audrey Ponce</creatorcontrib><creatorcontrib>YAMIN LUO</creatorcontrib><creatorcontrib>JORGENSEN, Jeffrey</creatorcontrib><creatorcontrib>SCHWARTZ, Mary R</creatorcontrib><creatorcontrib>CHANG, Chung-Che</creatorcontrib><title>Restricted κ/λ light chain ratio by flow cytometry in germinal center B cells in hashimoto thyroiditis</title><title>American journal of clinical pathology</title><addtitle>Am J Clin Pathol</addtitle><description>To determine the diagnostic significance of the kappa/lambda ratio in germinal center (GC) B cells in Hashimoto thyroiditis (HT), we used 4-color flow cytometry to immunophenotype 27 samples (21 patients) of well-characterized HT B-cell clonality was analyzed further by polymerase chain reaction (PCR) of the immunoglobulin heavy chain (IgH) and bcl-2/IgH fusion genes using DNA extracted from aspirate smears and/or paraffin-embedded tissues. By flow cytometric analysis, the CD10+ GC B cells had a higher mean +/- SD kappa/lambda ratio than the CD10- B cells (5.1 +/- 3.3 vs 2.0 +/- 0.8; P < .0001, Student t test). In 18 samples (67%), CD10+ GC B cells had a kappa/lambda ratio greater than 3.07 (the upper limit of kappa/lambda ratio reported in reactive nodes; range, 3.2-14.4 in the 18 cases). Cases tested by PCR showed no evidence of a clonal proliferation. None of 21 cases developed lymphoma during clinical follow-up of up to 3 years. The kappa/lambda ratio of CD10+ GC B cells in HT can be skewed markedly beyond that reported in reactive lymph nodes. This finding frequently is present in HT. Pathologists should be familiar with this phenomenon to prevent misdiagnosis of follicular lymphoma in patients with HT.</description><subject>Adult</subject><subject>Aged</subject><subject>B-Lymphocytes - immunology</subject><subject>Biological and medical sciences</subject><subject>Clone Cells - immunology</subject><subject>Endocrinopathies</subject><subject>Female</subject><subject>Flow Cytometry - methods</subject><subject>Gene Rearrangement, B-Lymphocyte, Heavy Chain</subject><subject>Germinal Center - cytology</subject><subject>Hashimoto Disease - immunology</subject><subject>Hashimoto Disease - pathology</subject><subject>Humans</subject><subject>Immunoglobulin kappa-Chains - analysis</subject><subject>Immunoglobulin lambda-Chains - analysis</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Neprilysin - analysis</subject><subject>Non tumoral diseases. Target tissue resistance. Benign neoplasms</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Polymerase Chain Reaction</subject><subject>Retrospective Studies</subject><subject>Thyroid Gland - immunology</subject><subject>Thyroid Gland - pathology</subject><subject>Thyroid. Thyroid axis (diseases)</subject><issn>0002-9173</issn><issn>1943-7722</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkEtLxDAUhYMozji6dyXZ6K5O0rwmS98KPkB04aqkaTKNtI0mGaR_Tf_D_CY7zIDg6l643znccwA4xOgUEySn-IZdS5S_XYnLy9fHh7MtMMaSkkyIPN8GY4RQnkksyAjsxfiOEM5niO6CEeZ0lkuJxqB-NjEFp5Op4PJ7uvyBjZvXCepauQ4GlZyHZQ9t47-g7pNvTQo9HE5zE1rXqQZq0yUT4PmwNE1cnWoVa9f65GGq--Bd5ZKL-2DHqiaag82cgNfrq5eL2-z-6ebu4uw-04TLlClkMGEzWs5yY7Ed0ggttdLaUFlhjkqNKswY5yXCDDMrSmN0WVZWVtxWJCcTcLL2_Qj-czGEK1oXV6-pzvhFLLjgnDJGBxCtQR18jMHY4iO4VoW-wKhYtVv8b3eQHG28F2Vrqj_Bps4BON4AKmrV2KA67eIfJ6gQjFDyC6DChmY</recordid><startdate>2006</startdate><enddate>2006</enddate><creator>CHEN, Henry I</creator><creator>AKPOLAT, Ikser</creator><creator>MODY, Dina R</creator><creator>LOPEZ-TERRADA, Dolores</creator><creator>DE LEON, Audrey Ponce</creator><creator>YAMIN LUO</creator><creator>JORGENSEN, Jeffrey</creator><creator>SCHWARTZ, Mary R</creator><creator>CHANG, Chung-Che</creator><general>American Society of Clinical Pathologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2006</creationdate><title>Restricted κ/λ light chain ratio by flow cytometry in germinal center B cells in hashimoto thyroiditis</title><author>CHEN, Henry I ; AKPOLAT, Ikser ; MODY, Dina R ; LOPEZ-TERRADA, Dolores ; DE LEON, Audrey Ponce ; YAMIN LUO ; JORGENSEN, Jeffrey ; SCHWARTZ, Mary R ; CHANG, Chung-Che</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c369t-a0e13584b82ef1f1947c9cacce49d160bc0d15566b01515f7beecbbdf9d6fd323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adult</topic><topic>Aged</topic><topic>B-Lymphocytes - immunology</topic><topic>Biological and medical sciences</topic><topic>Clone Cells - immunology</topic><topic>Endocrinopathies</topic><topic>Female</topic><topic>Flow Cytometry - methods</topic><topic>Gene Rearrangement, B-Lymphocyte, Heavy Chain</topic><topic>Germinal Center - cytology</topic><topic>Hashimoto Disease - immunology</topic><topic>Hashimoto Disease - pathology</topic><topic>Humans</topic><topic>Immunoglobulin kappa-Chains - analysis</topic><topic>Immunoglobulin lambda-Chains - analysis</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Neprilysin - analysis</topic><topic>Non tumoral diseases. Target tissue resistance. Benign neoplasms</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Polymerase Chain Reaction</topic><topic>Retrospective Studies</topic><topic>Thyroid Gland - immunology</topic><topic>Thyroid Gland - pathology</topic><topic>Thyroid. Thyroid axis (diseases)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHEN, Henry I</creatorcontrib><creatorcontrib>AKPOLAT, Ikser</creatorcontrib><creatorcontrib>MODY, Dina R</creatorcontrib><creatorcontrib>LOPEZ-TERRADA, Dolores</creatorcontrib><creatorcontrib>DE LEON, Audrey Ponce</creatorcontrib><creatorcontrib>YAMIN LUO</creatorcontrib><creatorcontrib>JORGENSEN, Jeffrey</creatorcontrib><creatorcontrib>SCHWARTZ, Mary R</creatorcontrib><creatorcontrib>CHANG, Chung-Che</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CHEN, Henry I</au><au>AKPOLAT, Ikser</au><au>MODY, Dina R</au><au>LOPEZ-TERRADA, Dolores</au><au>DE LEON, Audrey Ponce</au><au>YAMIN LUO</au><au>JORGENSEN, Jeffrey</au><au>SCHWARTZ, Mary R</au><au>CHANG, Chung-Che</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Restricted κ/λ light chain ratio by flow cytometry in germinal center B cells in hashimoto thyroiditis</atitle><jtitle>American journal of clinical pathology</jtitle><addtitle>Am J Clin Pathol</addtitle><date>2006</date><risdate>2006</risdate><volume>125</volume><issue>1</issue><spage>42</spage><epage>48</epage><pages>42-48</pages><issn>0002-9173</issn><eissn>1943-7722</eissn><coden>AJCPAI</coden><abstract>To determine the diagnostic significance of the kappa/lambda ratio in germinal center (GC) B cells in Hashimoto thyroiditis (HT), we used 4-color flow cytometry to immunophenotype 27 samples (21 patients) of well-characterized HT B-cell clonality was analyzed further by polymerase chain reaction (PCR) of the immunoglobulin heavy chain (IgH) and bcl-2/IgH fusion genes using DNA extracted from aspirate smears and/or paraffin-embedded tissues. By flow cytometric analysis, the CD10+ GC B cells had a higher mean +/- SD kappa/lambda ratio than the CD10- B cells (5.1 +/- 3.3 vs 2.0 +/- 0.8; P < .0001, Student t test). In 18 samples (67%), CD10+ GC B cells had a kappa/lambda ratio greater than 3.07 (the upper limit of kappa/lambda ratio reported in reactive nodes; range, 3.2-14.4 in the 18 cases). Cases tested by PCR showed no evidence of a clonal proliferation. None of 21 cases developed lymphoma during clinical follow-up of up to 3 years. The kappa/lambda ratio of CD10+ GC B cells in HT can be skewed markedly beyond that reported in reactive lymph nodes. This finding frequently is present in HT. Pathologists should be familiar with this phenomenon to prevent misdiagnosis of follicular lymphoma in patients with HT.</abstract><cop>Chicago, IL</cop><pub>American Society of Clinical Pathologists</pub><pmid>16482990</pmid><doi>10.1309/1G5F902YE7DDUNMA</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | American journal of clinical pathology, 2006, Vol.125 (1), p.42-48 |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Adult Aged B-Lymphocytes - immunology Biological and medical sciences Clone Cells - immunology Endocrinopathies Female Flow Cytometry - methods Gene Rearrangement, B-Lymphocyte, Heavy Chain Germinal Center - cytology Hashimoto Disease - immunology Hashimoto Disease - pathology Humans Immunoglobulin kappa-Chains - analysis Immunoglobulin lambda-Chains - analysis Investigative techniques, diagnostic techniques (general aspects) Medical sciences Middle Aged Neprilysin - analysis Non tumoral diseases. Target tissue resistance. Benign neoplasms Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Polymerase Chain Reaction Retrospective Studies Thyroid Gland - immunology Thyroid Gland - pathology Thyroid. Thyroid axis (diseases) |
| title | Restricted κ/λ light chain ratio by flow cytometry in germinal center B cells in hashimoto thyroiditis |
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