Chromatographic Determination of Fe Chelated by Ethylenediamine-N-(o-hydroxyphenylacetic)-N‘-(p-hydroxyphenylacetic) Acid in Commercial EDDHA/Fe3+ Fertilizers
EDDHA/Fe3+ chelates are the most common fertilizers used to solve Fe chlorosis in established crops. Commercial products contain two regioisomers, ethylenediamine-N,N‘-bis(o-hydroxyphenylacetic) acid (o,o-EDDHA)/Fe3+ and ethylenediamine-N-(o-hydroxyphenylacetic)-N‘-(p-hydroxyphenylacetic) acid (o,p-...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2006-02, Vol.54 (4), p.1380-1386 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | García-Marco, Sonia Torreblanca, Ana Lucena, Juan J |
| description | EDDHA/Fe3+ chelates are the most common fertilizers used to solve Fe chlorosis in established crops. Commercial products contain two regioisomers, ethylenediamine-N,N‘-bis(o-hydroxyphenylacetic) acid (o,o-EDDHA)/Fe3+ and ethylenediamine-N-(o-hydroxyphenylacetic)-N‘-(p-hydroxyphenylacetic) acid (o,p-EDDHA)/Fe3+. Although several chromatographic methods exist for the determination of Fe3+ chelated by the o,o-EDDHA isomer, no method has been described for the quantification of Fe3+ chelated by o,p-EDDHA. In this work, factors that affect the behavior of o,p-EDDHA/Fe3+ in ion pair chromatography are reviewed: pH, ion pair reagent, and organic modifier. The best chromatographic performance was obtained with an aqueous mobile phase at pH 6.0 containing 35% acetonitrile and 5 mM tetrabutylammonium hydroxide under isocratic elution conditions. This method was applied to the quantification of commercial samples. Keywords: EDDHA/Fe3+; Fe3+ chelates; fertilizers; HPLC; o,o-EDDHA/Fe3+; o,p-EDDHA/Fe3+ |
| doi_str_mv | 10.1021/jf051745x |
| format | Article |
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Commercial products contain two regioisomers, ethylenediamine-N,N‘-bis(o-hydroxyphenylacetic) acid (o,o-EDDHA)/Fe3+ and ethylenediamine-N-(o-hydroxyphenylacetic)-N‘-(p-hydroxyphenylacetic) acid (o,p-EDDHA)/Fe3+. Although several chromatographic methods exist for the determination of Fe3+ chelated by the o,o-EDDHA isomer, no method has been described for the quantification of Fe3+ chelated by o,p-EDDHA. In this work, factors that affect the behavior of o,p-EDDHA/Fe3+ in ion pair chromatography are reviewed: pH, ion pair reagent, and organic modifier. The best chromatographic performance was obtained with an aqueous mobile phase at pH 6.0 containing 35% acetonitrile and 5 mM tetrabutylammonium hydroxide under isocratic elution conditions. This method was applied to the quantification of commercial samples. Keywords: EDDHA/Fe3+; Fe3+ chelates; fertilizers; HPLC; o,o-EDDHA/Fe3+; o,p-EDDHA/Fe3+</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf051745x</identifier><identifier>PMID: 16478263</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Biological and medical sciences ; chelates ; chelating agents ; chromatography ; Chromatography, High Pressure Liquid ; EDDHA ; Ethylenediamines - analysis ; Ethylenediamines - chemistry ; Ferric Compounds - analysis ; Ferric Compounds - chemistry ; Fertilizers - analysis ; Food industries ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; ion pair chromatography ; iron ; Iron Chelating Agents - chemistry ; iron fertilizers ; mineral content</subject><ispartof>Journal of agricultural and food chemistry, 2006-02, Vol.54 (4), p.1380-1386</ispartof><rights>Copyright © 2006 American Chemical Society</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf051745x$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf051745x$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17514606$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16478263$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>García-Marco, Sonia</creatorcontrib><creatorcontrib>Torreblanca, Ana</creatorcontrib><creatorcontrib>Lucena, Juan J</creatorcontrib><title>Chromatographic Determination of Fe Chelated by Ethylenediamine-N-(o-hydroxyphenylacetic)-N‘-(p-hydroxyphenylacetic) Acid in Commercial EDDHA/Fe3+ Fertilizers</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>EDDHA/Fe3+ chelates are the most common fertilizers used to solve Fe chlorosis in established crops. Commercial products contain two regioisomers, ethylenediamine-N,N‘-bis(o-hydroxyphenylacetic) acid (o,o-EDDHA)/Fe3+ and ethylenediamine-N-(o-hydroxyphenylacetic)-N‘-(p-hydroxyphenylacetic) acid (o,p-EDDHA)/Fe3+. Although several chromatographic methods exist for the determination of Fe3+ chelated by the o,o-EDDHA isomer, no method has been described for the quantification of Fe3+ chelated by o,p-EDDHA. In this work, factors that affect the behavior of o,p-EDDHA/Fe3+ in ion pair chromatography are reviewed: pH, ion pair reagent, and organic modifier. The best chromatographic performance was obtained with an aqueous mobile phase at pH 6.0 containing 35% acetonitrile and 5 mM tetrabutylammonium hydroxide under isocratic elution conditions. This method was applied to the quantification of commercial samples. Keywords: EDDHA/Fe3+; Fe3+ chelates; fertilizers; HPLC; o,o-EDDHA/Fe3+; o,p-EDDHA/Fe3+</description><subject>Biological and medical sciences</subject><subject>chelates</subject><subject>chelating agents</subject><subject>chromatography</subject><subject>Chromatography, High Pressure Liquid</subject><subject>EDDHA</subject><subject>Ethylenediamines - analysis</subject><subject>Ethylenediamines - chemistry</subject><subject>Ferric Compounds - analysis</subject><subject>Ferric Compounds - chemistry</subject><subject>Fertilizers - analysis</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>ion pair chromatography</subject><subject>iron</subject><subject>Iron Chelating Agents - chemistry</subject><subject>iron fertilizers</subject><subject>mineral content</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0sGO0zAQBuAIgdiycOAFwBfQImTWTjKOe6zSlkWUBbS74mhNHGfjbhIXO5UaTjwGR56NJyHQsnDgNIf_0_zSaKLoMWevOIv56bpiwLMUdneiCYeYUeBc3o0mbAypBMGPogchrBljEjJ2PzriIs1kLJJJ9D2vvWuxd9ceN7XVZG5641vbYW9dR1xFlobktWmwNyUpBrLo66ExnSktjsrQc3riaD2U3u2GTW26oUFteqtf0PMfX7_Rk81_QzLTtiS2I7lrW-O1xYYs5vOz2enSJC_HTt_bxn4xPjyM7lXYBPPoMI-jq-XiMj-jq_ev3-SzFUWeiYSWoFMpRRpP-RRkiVOdgswExyrhkLJYViiFKGCagS5EwUEIkckUCgkAmCbJcfR8v3fj3eetCb1qbdCmabAzbhuUyIRI-G_45AC3RWtKtfG2RT-oPzcdwbMDwKCxqTx22oa_LgOeCiZGR_fOht7sbnP0N2NZkoG6_HChPn56F7-FVaxg9E_3vkKn8NqPO68uYsYTxplgLP2nGXVQa7f13XgxxZn69SXq9kuSnzriqV8</recordid><startdate>20060222</startdate><enddate>20060222</enddate><creator>García-Marco, Sonia</creator><creator>Torreblanca, Ana</creator><creator>Lucena, Juan J</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20060222</creationdate><title>Chromatographic Determination of Fe Chelated by Ethylenediamine-N-(o-hydroxyphenylacetic)-N‘-(p-hydroxyphenylacetic) Acid in Commercial EDDHA/Fe3+ Fertilizers</title><author>García-Marco, Sonia ; Torreblanca, Ana ; Lucena, Juan J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a1763-d5c48864291958da9c458761af3154028fa866b5975cb6b156667845b8555a433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Biological and medical sciences</topic><topic>chelates</topic><topic>chelating agents</topic><topic>chromatography</topic><topic>Chromatography, High Pressure Liquid</topic><topic>EDDHA</topic><topic>Ethylenediamines - analysis</topic><topic>Ethylenediamines - chemistry</topic><topic>Ferric Compounds - analysis</topic><topic>Ferric Compounds - chemistry</topic><topic>Fertilizers - analysis</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>ion pair chromatography</topic><topic>iron</topic><topic>Iron Chelating Agents - chemistry</topic><topic>iron fertilizers</topic><topic>mineral content</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>García-Marco, Sonia</creatorcontrib><creatorcontrib>Torreblanca, Ana</creatorcontrib><creatorcontrib>Lucena, Juan J</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>García-Marco, Sonia</au><au>Torreblanca, Ana</au><au>Lucena, Juan J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chromatographic Determination of Fe Chelated by Ethylenediamine-N-(o-hydroxyphenylacetic)-N‘-(p-hydroxyphenylacetic) Acid in Commercial EDDHA/Fe3+ Fertilizers</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2006-02-22</date><risdate>2006</risdate><volume>54</volume><issue>4</issue><spage>1380</spage><epage>1386</epage><pages>1380-1386</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>EDDHA/Fe3+ chelates are the most common fertilizers used to solve Fe chlorosis in established crops. Commercial products contain two regioisomers, ethylenediamine-N,N‘-bis(o-hydroxyphenylacetic) acid (o,o-EDDHA)/Fe3+ and ethylenediamine-N-(o-hydroxyphenylacetic)-N‘-(p-hydroxyphenylacetic) acid (o,p-EDDHA)/Fe3+. Although several chromatographic methods exist for the determination of Fe3+ chelated by the o,o-EDDHA isomer, no method has been described for the quantification of Fe3+ chelated by o,p-EDDHA. In this work, factors that affect the behavior of o,p-EDDHA/Fe3+ in ion pair chromatography are reviewed: pH, ion pair reagent, and organic modifier. The best chromatographic performance was obtained with an aqueous mobile phase at pH 6.0 containing 35% acetonitrile and 5 mM tetrabutylammonium hydroxide under isocratic elution conditions. This method was applied to the quantification of commercial samples. Keywords: EDDHA/Fe3+; Fe3+ chelates; fertilizers; HPLC; o,o-EDDHA/Fe3+; o,p-EDDHA/Fe3+</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>16478263</pmid><doi>10.1021/jf051745x</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of agricultural and food chemistry, 2006-02, Vol.54 (4), p.1380-1386 |
| issn | 0021-8561 1520-5118 |
| language | eng |
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| subjects | Biological and medical sciences chelates chelating agents chromatography Chromatography, High Pressure Liquid EDDHA Ethylenediamines - analysis Ethylenediamines - chemistry Ferric Compounds - analysis Ferric Compounds - chemistry Fertilizers - analysis Food industries Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration ion pair chromatography iron Iron Chelating Agents - chemistry iron fertilizers mineral content |
| title | Chromatographic Determination of Fe Chelated by Ethylenediamine-N-(o-hydroxyphenylacetic)-N‘-(p-hydroxyphenylacetic) Acid in Commercial EDDHA/Fe3+ Fertilizers |
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