Gene expression profiling reveals that peripheral nerve regeneration is a consequence of both novel injury‐dependent and reactivated developmental processes
One of the most striking features of the injured mature peripheral nervous system is the ability to regenerate. The lesioned peripheral nervous system displays stereotypic histopathological reactions indicating the activation of a co‐ordinated lesion‐induced gene expression programme. Previous resea...
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| Veröffentlicht in: | Journal of neurochemistry 2006-03, Vol.96 (5), p.1441-1457 |
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| creator | Bosse, Frank Hasenpusch‐Theil, Kerstin Küry, Patrick Müller, Hans Werner |
| description | One of the most striking features of the injured mature peripheral nervous system is the ability to regenerate. The lesioned peripheral nervous system displays stereotypic histopathological reactions indicating the activation of a co‐ordinated lesion‐induced gene expression programme. Previous research has already identified molecular components of this axonal switch from a mature transmitting to a regenerative growth mode. The observed alterations in gene expression within the lesioned distal nerve stump were largely attributed to recapitulated developmental processes. However, to our knowledge, this hypothesis has not been proven systematically. Most of the stereotypic molecular and cellular reactions during nerve development and repair can be assigned to specific time windows. Consequently, we have compared gene expression profiles of both paradigms at six different time‐points each by means of cDNA array hybridization. Our data identified injury‐specific molecular reactions and revealed to what extent developmental mechanisms are reactivated in response to nerve lesion. Ninety‐one genes (47% of the regeneration‐associated genes) were found to be significantly regulated in both paradigms, suggesting that regeneration only partially recapitulates development and that approximately half of the regulated genes are part of a regeneration‐dependent programme. Interestingly, mainly genes encoding signal transducers or factors involved in processes such as cell death, immune response, transport and transcriptional regulation showed injury‐specific gene expression. |
| doi_str_mv | 10.1111/j.1471-4159.2005.03635.x |
| format | Article |
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The lesioned peripheral nervous system displays stereotypic histopathological reactions indicating the activation of a co‐ordinated lesion‐induced gene expression programme. Previous research has already identified molecular components of this axonal switch from a mature transmitting to a regenerative growth mode. The observed alterations in gene expression within the lesioned distal nerve stump were largely attributed to recapitulated developmental processes. However, to our knowledge, this hypothesis has not been proven systematically. Most of the stereotypic molecular and cellular reactions during nerve development and repair can be assigned to specific time windows. Consequently, we have compared gene expression profiles of both paradigms at six different time‐points each by means of cDNA array hybridization. Our data identified injury‐specific molecular reactions and revealed to what extent developmental mechanisms are reactivated in response to nerve lesion. Ninety‐one genes (47% of the regeneration‐associated genes) were found to be significantly regulated in both paradigms, suggesting that regeneration only partially recapitulates development and that approximately half of the regulated genes are part of a regeneration‐dependent programme. Interestingly, mainly genes encoding signal transducers or factors involved in processes such as cell death, immune response, transport and transcriptional regulation showed injury‐specific gene expression.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1111/j.1471-4159.2005.03635.x</identifier><identifier>PMID: 16478531</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Animals, Newborn ; Biological and medical sciences ; cDNA array analysis ; Cells, Cultured ; Colforsin - pharmacology ; development ; Disease Models, Animal ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression - drug effects ; Gene Expression - physiology ; Gene Expression Profiling ; Gene Expression Regulation - physiology ; Genes ; Immune system ; Injuries ; Injuries of the nervous system and the skull. Diseases due to physical agents ; Male ; Medical sciences ; nerve injury ; Nerve Regeneration - physiology ; Nervous system ; Oligonucleotide Array Sequence Analysis - methods ; Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ ; Rats ; Rats, Wistar ; Reverse Transcriptase Polymerase Chain Reaction - methods ; RNA, Messenger - biosynthesis ; Schwann Cells - drug effects ; sciatic nerve regeneration ; Sciatic Neuropathy - metabolism ; Signal transduction ; Time Factors ; Traumas. 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The lesioned peripheral nervous system displays stereotypic histopathological reactions indicating the activation of a co‐ordinated lesion‐induced gene expression programme. Previous research has already identified molecular components of this axonal switch from a mature transmitting to a regenerative growth mode. The observed alterations in gene expression within the lesioned distal nerve stump were largely attributed to recapitulated developmental processes. However, to our knowledge, this hypothesis has not been proven systematically. Most of the stereotypic molecular and cellular reactions during nerve development and repair can be assigned to specific time windows. Consequently, we have compared gene expression profiles of both paradigms at six different time‐points each by means of cDNA array hybridization. Our data identified injury‐specific molecular reactions and revealed to what extent developmental mechanisms are reactivated in response to nerve lesion. Ninety‐one genes (47% of the regeneration‐associated genes) were found to be significantly regulated in both paradigms, suggesting that regeneration only partially recapitulates development and that approximately half of the regulated genes are part of a regeneration‐dependent programme. Interestingly, mainly genes encoding signal transducers or factors involved in processes such as cell death, immune response, transport and transcriptional regulation showed injury‐specific gene expression.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Biological and medical sciences</subject><subject>cDNA array analysis</subject><subject>Cells, Cultured</subject><subject>Colforsin - pharmacology</subject><subject>development</subject><subject>Disease Models, Animal</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression - drug effects</subject><subject>Gene Expression - physiology</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation - physiology</subject><subject>Genes</subject><subject>Immune system</subject><subject>Injuries</subject><subject>Injuries of the nervous system and the skull. Diseases due to physical agents</subject><subject>Male</subject><subject>Medical sciences</subject><subject>nerve injury</subject><subject>Nerve Regeneration - physiology</subject><subject>Nervous system</subject><subject>Oligonucleotide Array Sequence Analysis - methods</subject><subject>Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Schwann Cells - drug effects</subject><subject>sciatic nerve regeneration</subject><subject>Sciatic Neuropathy - metabolism</subject><subject>Signal transduction</subject><subject>Time Factors</subject><subject>Traumas. Diseases due to physical agents</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU2O1DAQhSMEYpqBKyALCXYJ_o2dBQvUggE0gg2sLcepTLuVtoOd9HTvOAIn4HCcBIduMRIb8KYs1feqXukVBSK4Ivm93FaES1JyIpqKYiwqzGomqsO9YvWncb9YYUxpyTCnF8WjlLYYk5rX5GFxkatUgpFV8eMKPCA4jBFScsGjMYbeDc7foAh7MENC08ZMaIToxg1EMyAPcQ-5e5OV0UyLyCVkkA0-wdcZvAUUetSGaYN82MOAnN_O8fjz2_cORvAd-AkZ3-URxk5ubyboUJeXDWHc5V5ekU3Y7AfS4-JBnz3Ak3O9LL68ffN5_a68_nT1fv36urRcCFE2WHHFWNe1WFCiGtWzvpW84w3uG6kYJgpYqwSlICw0BrBktJGgeEsAC2CXxYvT3Lw5n5AmvXPJwjAYD2FOupZ1TTlv_gkSSQSVUmTw2V_gNszR5yM0xbXgTCmaIXWCbAwpRej1GN3OxKMmWC9J661eAtVLoHpJWv9OWh-y9Ol5_tzuoLsTnqPNwPMzYJI1Qx-Nty7dcdkjrxuZuVcn7tYNcPxvA_rDx_XyY78AYDzH0g</recordid><startdate>200603</startdate><enddate>200603</enddate><creator>Bosse, Frank</creator><creator>Hasenpusch‐Theil, Kerstin</creator><creator>Küry, Patrick</creator><creator>Müller, Hans Werner</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200603</creationdate><title>Gene expression profiling reveals that peripheral nerve regeneration is a consequence of both novel injury‐dependent and reactivated developmental processes</title><author>Bosse, Frank ; Hasenpusch‐Theil, Kerstin ; Küry, Patrick ; Müller, Hans Werner</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4555-9084833ddb0521898f3fb74d490f9783018e3b8522e5ce9ae073297e84b1e05e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Biological and medical sciences</topic><topic>cDNA array analysis</topic><topic>Cells, Cultured</topic><topic>Colforsin - pharmacology</topic><topic>development</topic><topic>Disease Models, Animal</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression - drug effects</topic><topic>Gene Expression - physiology</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation - physiology</topic><topic>Genes</topic><topic>Immune system</topic><topic>Injuries</topic><topic>Injuries of the nervous system and the skull. Diseases due to physical agents</topic><topic>Male</topic><topic>Medical sciences</topic><topic>nerve injury</topic><topic>Nerve Regeneration - physiology</topic><topic>Nervous system</topic><topic>Oligonucleotide Array Sequence Analysis - methods</topic><topic>Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Schwann Cells - drug effects</topic><topic>sciatic nerve regeneration</topic><topic>Sciatic Neuropathy - metabolism</topic><topic>Signal transduction</topic><topic>Time Factors</topic><topic>Traumas. Diseases due to physical agents</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bosse, Frank</creatorcontrib><creatorcontrib>Hasenpusch‐Theil, Kerstin</creatorcontrib><creatorcontrib>Küry, Patrick</creatorcontrib><creatorcontrib>Müller, Hans Werner</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bosse, Frank</au><au>Hasenpusch‐Theil, Kerstin</au><au>Küry, Patrick</au><au>Müller, Hans Werner</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene expression profiling reveals that peripheral nerve regeneration is a consequence of both novel injury‐dependent and reactivated developmental processes</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>2006-03</date><risdate>2006</risdate><volume>96</volume><issue>5</issue><spage>1441</spage><epage>1457</epage><pages>1441-1457</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>One of the most striking features of the injured mature peripheral nervous system is the ability to regenerate. The lesioned peripheral nervous system displays stereotypic histopathological reactions indicating the activation of a co‐ordinated lesion‐induced gene expression programme. Previous research has already identified molecular components of this axonal switch from a mature transmitting to a regenerative growth mode. The observed alterations in gene expression within the lesioned distal nerve stump were largely attributed to recapitulated developmental processes. However, to our knowledge, this hypothesis has not been proven systematically. Most of the stereotypic molecular and cellular reactions during nerve development and repair can be assigned to specific time windows. Consequently, we have compared gene expression profiles of both paradigms at six different time‐points each by means of cDNA array hybridization. Our data identified injury‐specific molecular reactions and revealed to what extent developmental mechanisms are reactivated in response to nerve lesion. Ninety‐one genes (47% of the regeneration‐associated genes) were found to be significantly regulated in both paradigms, suggesting that regeneration only partially recapitulates development and that approximately half of the regulated genes are part of a regeneration‐dependent programme. Interestingly, mainly genes encoding signal transducers or factors involved in processes such as cell death, immune response, transport and transcriptional regulation showed injury‐specific gene expression.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16478531</pmid><doi>10.1111/j.1471-4159.2005.03635.x</doi><tpages>17</tpages></addata></record> |
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| subjects | Animals Animals, Newborn Biological and medical sciences cDNA array analysis Cells, Cultured Colforsin - pharmacology development Disease Models, Animal Fundamental and applied biological sciences. Psychology Gene expression Gene Expression - drug effects Gene Expression - physiology Gene Expression Profiling Gene Expression Regulation - physiology Genes Immune system Injuries Injuries of the nervous system and the skull. Diseases due to physical agents Male Medical sciences nerve injury Nerve Regeneration - physiology Nervous system Oligonucleotide Array Sequence Analysis - methods Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Messenger - biosynthesis Schwann Cells - drug effects sciatic nerve regeneration Sciatic Neuropathy - metabolism Signal transduction Time Factors Traumas. Diseases due to physical agents Vertebrates: nervous system and sense organs |
| title | Gene expression profiling reveals that peripheral nerve regeneration is a consequence of both novel injury‐dependent and reactivated developmental processes |
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