Preservation of Rat Cremaster Muscle Microcirculation after Prolonged Cold Storage and Transplantation
Microvascular surgery for the reconstruction of complex defects involves an ischemic period, which may cause flap failure as the result of ischemia/reperfusion injury. We assessed the microvascular consequences of rat cremaster muscle transplantation after prolonged periods of cold storage in HTK-Br...
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Veröffentlicht in: | The Journal of surgical research 2006-03, Vol.131 (1), p.41-48 |
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creator | Bastiaanse, Jacqueline Nanhekhan, Lloyd V. Slaaf, Dick W. Boeckx, Willy D. oude Egbrink, Mirjam G.A. |
description | Microvascular surgery for the reconstruction of complex defects involves an ischemic period, which may cause flap failure as the result of ischemia/reperfusion injury. We assessed the microvascular consequences of rat cremaster muscle transplantation after prolonged periods of cold storage in HTK-Bretschneider solution (HTK).
Cremaster muscle transplantations were performed immediately or after 8 or 24 h of cold storage (4°C) in HTK or saline. Intravital microscopy was used to quantify capillary perfusion and venular leukocyte-endothelium interactions following transplantation.
The transplantation procedure itself resulted in 50–65 min of ischemia. After direct transplantation, capillary perfusion was 90% of control. Transplantation after 8 h of cold storage in either HTK or saline did not deteriorate capillary perfusion. When the tissue was stored for 24 h, HTK was superior to saline in preserving capillary perfusion (HTK: 76–83% of control, saline: 30%). Immediate transplantation induced a small increase in leukocyte adhesion. Prolonged cold storage in either fluid resulted in reduced flow velocities (qualitative observations) and edema formation, which hampered quantification of leukocyte-endothelium interactions.
Even after 8 or 24 h of cold storage in HTK, transplantation of rat cremaster muscle was successful with good capillary perfusion. Capillary perfusion was better preserved in HTK than in saline. |
doi_str_mv | 10.1016/j.jss.2005.05.027 |
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Cremaster muscle transplantations were performed immediately or after 8 or 24 h of cold storage (4°C) in HTK or saline. Intravital microscopy was used to quantify capillary perfusion and venular leukocyte-endothelium interactions following transplantation.
The transplantation procedure itself resulted in 50–65 min of ischemia. After direct transplantation, capillary perfusion was 90% of control. Transplantation after 8 h of cold storage in either HTK or saline did not deteriorate capillary perfusion. When the tissue was stored for 24 h, HTK was superior to saline in preserving capillary perfusion (HTK: 76–83% of control, saline: 30%). Immediate transplantation induced a small increase in leukocyte adhesion. Prolonged cold storage in either fluid resulted in reduced flow velocities (qualitative observations) and edema formation, which hampered quantification of leukocyte-endothelium interactions.
Even after 8 or 24 h of cold storage in HTK, transplantation of rat cremaster muscle was successful with good capillary perfusion. Capillary perfusion was better preserved in HTK than in saline.</description><identifier>ISSN: 0022-4804</identifier><identifier>EISSN: 1095-8673</identifier><identifier>DOI: 10.1016/j.jss.2005.05.027</identifier><identifier>PMID: 16054649</identifier><identifier>CODEN: JSGRA2</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Cell Adhesion ; Edema ; General aspects ; Glucose ; HTK ; Ischemia ; ischemia reperfusion injury ; Leukocytes ; Male ; Mannitol ; Medical sciences ; Microcirculation ; Muscle, Skeletal - blood supply ; Muscle, Skeletal - transplantation ; Potassium Chloride ; preservation ; Procaine ; Rats ; Reperfusion ; Sodium Chloride ; Specimen Handling ; Tissue Preservation - methods ; transplantation</subject><ispartof>The Journal of surgical research, 2006-03, Vol.131 (1), p.41-48</ispartof><rights>2006 Elsevier Inc.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-ae27f9dfca4b48f5e97f28cf66b5e0748ca51e105446d03a80b3a174715880e53</citedby><cites>FETCH-LOGICAL-c381t-ae27f9dfca4b48f5e97f28cf66b5e0748ca51e105446d03a80b3a174715880e53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jss.2005.05.027$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17561169$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16054649$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bastiaanse, Jacqueline</creatorcontrib><creatorcontrib>Nanhekhan, Lloyd V.</creatorcontrib><creatorcontrib>Slaaf, Dick W.</creatorcontrib><creatorcontrib>Boeckx, Willy D.</creatorcontrib><creatorcontrib>oude Egbrink, Mirjam G.A.</creatorcontrib><title>Preservation of Rat Cremaster Muscle Microcirculation after Prolonged Cold Storage and Transplantation</title><title>The Journal of surgical research</title><addtitle>J Surg Res</addtitle><description>Microvascular surgery for the reconstruction of complex defects involves an ischemic period, which may cause flap failure as the result of ischemia/reperfusion injury. We assessed the microvascular consequences of rat cremaster muscle transplantation after prolonged periods of cold storage in HTK-Bretschneider solution (HTK).
Cremaster muscle transplantations were performed immediately or after 8 or 24 h of cold storage (4°C) in HTK or saline. Intravital microscopy was used to quantify capillary perfusion and venular leukocyte-endothelium interactions following transplantation.
The transplantation procedure itself resulted in 50–65 min of ischemia. After direct transplantation, capillary perfusion was 90% of control. Transplantation after 8 h of cold storage in either HTK or saline did not deteriorate capillary perfusion. When the tissue was stored for 24 h, HTK was superior to saline in preserving capillary perfusion (HTK: 76–83% of control, saline: 30%). Immediate transplantation induced a small increase in leukocyte adhesion. Prolonged cold storage in either fluid resulted in reduced flow velocities (qualitative observations) and edema formation, which hampered quantification of leukocyte-endothelium interactions.
Even after 8 or 24 h of cold storage in HTK, transplantation of rat cremaster muscle was successful with good capillary perfusion. Capillary perfusion was better preserved in HTK than in saline.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Adhesion</subject><subject>Edema</subject><subject>General aspects</subject><subject>Glucose</subject><subject>HTK</subject><subject>Ischemia</subject><subject>ischemia reperfusion injury</subject><subject>Leukocytes</subject><subject>Male</subject><subject>Mannitol</subject><subject>Medical sciences</subject><subject>Microcirculation</subject><subject>Muscle, Skeletal - blood supply</subject><subject>Muscle, Skeletal - transplantation</subject><subject>Potassium Chloride</subject><subject>preservation</subject><subject>Procaine</subject><subject>Rats</subject><subject>Reperfusion</subject><subject>Sodium Chloride</subject><subject>Specimen Handling</subject><subject>Tissue Preservation - methods</subject><subject>transplantation</subject><issn>0022-4804</issn><issn>1095-8673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1LHDEUhoO01NX2B3hTctPezZrM5GvwSpZWBUVRex3OZk4ky-xkm8wI_fdmugveCQdCyPMe3jyEnHG25Iyr881yk_OyZkwu56n1EVlw1srKKN18IgvG6roSholjcpLzhpV7q5sv5JgrJoUS7YL4h4QZ0yuMIQ40evoII10l3EIeMdG7Kbse6V1wKbqQ3NTvQfDz60OKfRxesKOr2Hf0aYwJXpDC0NHnBEPe9TCM_wNfyWcPfcZvh_OU_Pn963l1Xd3eX92sLm8r1xg-VoC19m3nHYi1MF5iq31tnFdqLZFpYRxIjryUF6pjDRi2boBrobk0hqFsTsnP_d5din8nzKPdhuywL0UwTtkqraRpOSsg34PlYzkn9HaXwhbSP8uZneXajS1y7SzXzlPrkvl-WD6tt9i9Jw42C_DjAEB20PviwIX8zmmpOFczd7HnsKh4DZhsdgEHh11I6EbbxfBBjTfjJZh6</recordid><startdate>20060301</startdate><enddate>20060301</enddate><creator>Bastiaanse, Jacqueline</creator><creator>Nanhekhan, Lloyd V.</creator><creator>Slaaf, Dick W.</creator><creator>Boeckx, Willy D.</creator><creator>oude Egbrink, Mirjam G.A.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060301</creationdate><title>Preservation of Rat Cremaster Muscle Microcirculation after Prolonged Cold Storage and Transplantation</title><author>Bastiaanse, Jacqueline ; Nanhekhan, Lloyd V. ; Slaaf, Dick W. ; Boeckx, Willy D. ; oude Egbrink, Mirjam G.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-ae27f9dfca4b48f5e97f28cf66b5e0748ca51e105446d03a80b3a174715880e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Adhesion</topic><topic>Edema</topic><topic>General aspects</topic><topic>Glucose</topic><topic>HTK</topic><topic>Ischemia</topic><topic>ischemia reperfusion injury</topic><topic>Leukocytes</topic><topic>Male</topic><topic>Mannitol</topic><topic>Medical sciences</topic><topic>Microcirculation</topic><topic>Muscle, Skeletal - blood supply</topic><topic>Muscle, Skeletal - transplantation</topic><topic>Potassium Chloride</topic><topic>preservation</topic><topic>Procaine</topic><topic>Rats</topic><topic>Reperfusion</topic><topic>Sodium Chloride</topic><topic>Specimen Handling</topic><topic>Tissue Preservation - methods</topic><topic>transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bastiaanse, Jacqueline</creatorcontrib><creatorcontrib>Nanhekhan, Lloyd V.</creatorcontrib><creatorcontrib>Slaaf, Dick W.</creatorcontrib><creatorcontrib>Boeckx, Willy D.</creatorcontrib><creatorcontrib>oude Egbrink, Mirjam G.A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of surgical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bastiaanse, Jacqueline</au><au>Nanhekhan, Lloyd V.</au><au>Slaaf, Dick W.</au><au>Boeckx, Willy D.</au><au>oude Egbrink, Mirjam G.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preservation of Rat Cremaster Muscle Microcirculation after Prolonged Cold Storage and Transplantation</atitle><jtitle>The Journal of surgical research</jtitle><addtitle>J Surg Res</addtitle><date>2006-03-01</date><risdate>2006</risdate><volume>131</volume><issue>1</issue><spage>41</spage><epage>48</epage><pages>41-48</pages><issn>0022-4804</issn><eissn>1095-8673</eissn><coden>JSGRA2</coden><abstract>Microvascular surgery for the reconstruction of complex defects involves an ischemic period, which may cause flap failure as the result of ischemia/reperfusion injury. We assessed the microvascular consequences of rat cremaster muscle transplantation after prolonged periods of cold storage in HTK-Bretschneider solution (HTK).
Cremaster muscle transplantations were performed immediately or after 8 or 24 h of cold storage (4°C) in HTK or saline. Intravital microscopy was used to quantify capillary perfusion and venular leukocyte-endothelium interactions following transplantation.
The transplantation procedure itself resulted in 50–65 min of ischemia. After direct transplantation, capillary perfusion was 90% of control. Transplantation after 8 h of cold storage in either HTK or saline did not deteriorate capillary perfusion. When the tissue was stored for 24 h, HTK was superior to saline in preserving capillary perfusion (HTK: 76–83% of control, saline: 30%). Immediate transplantation induced a small increase in leukocyte adhesion. Prolonged cold storage in either fluid resulted in reduced flow velocities (qualitative observations) and edema formation, which hampered quantification of leukocyte-endothelium interactions.
Even after 8 or 24 h of cold storage in HTK, transplantation of rat cremaster muscle was successful with good capillary perfusion. Capillary perfusion was better preserved in HTK than in saline.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>16054649</pmid><doi>10.1016/j.jss.2005.05.027</doi><tpages>8</tpages></addata></record> |
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subjects | Animals Biological and medical sciences Cell Adhesion Edema General aspects Glucose HTK Ischemia ischemia reperfusion injury Leukocytes Male Mannitol Medical sciences Microcirculation Muscle, Skeletal - blood supply Muscle, Skeletal - transplantation Potassium Chloride preservation Procaine Rats Reperfusion Sodium Chloride Specimen Handling Tissue Preservation - methods transplantation |
title | Preservation of Rat Cremaster Muscle Microcirculation after Prolonged Cold Storage and Transplantation |
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