Preservation of Rat Cremaster Muscle Microcirculation after Prolonged Cold Storage and Transplantation

Microvascular surgery for the reconstruction of complex defects involves an ischemic period, which may cause flap failure as the result of ischemia/reperfusion injury. We assessed the microvascular consequences of rat cremaster muscle transplantation after prolonged periods of cold storage in HTK-Br...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The Journal of surgical research 2006-03, Vol.131 (1), p.41-48
Hauptverfasser: Bastiaanse, Jacqueline, Nanhekhan, Lloyd V., Slaaf, Dick W., Boeckx, Willy D., oude Egbrink, Mirjam G.A.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 48
container_issue 1
container_start_page 41
container_title The Journal of surgical research
container_volume 131
creator Bastiaanse, Jacqueline
Nanhekhan, Lloyd V.
Slaaf, Dick W.
Boeckx, Willy D.
oude Egbrink, Mirjam G.A.
description Microvascular surgery for the reconstruction of complex defects involves an ischemic period, which may cause flap failure as the result of ischemia/reperfusion injury. We assessed the microvascular consequences of rat cremaster muscle transplantation after prolonged periods of cold storage in HTK-Bretschneider solution (HTK). Cremaster muscle transplantations were performed immediately or after 8 or 24 h of cold storage (4°C) in HTK or saline. Intravital microscopy was used to quantify capillary perfusion and venular leukocyte-endothelium interactions following transplantation. The transplantation procedure itself resulted in 50–65 min of ischemia. After direct transplantation, capillary perfusion was 90% of control. Transplantation after 8 h of cold storage in either HTK or saline did not deteriorate capillary perfusion. When the tissue was stored for 24 h, HTK was superior to saline in preserving capillary perfusion (HTK: 76–83% of control, saline: 30%). Immediate transplantation induced a small increase in leukocyte adhesion. Prolonged cold storage in either fluid resulted in reduced flow velocities (qualitative observations) and edema formation, which hampered quantification of leukocyte-endothelium interactions. Even after 8 or 24 h of cold storage in HTK, transplantation of rat cremaster muscle was successful with good capillary perfusion. Capillary perfusion was better preserved in HTK than in saline.
doi_str_mv 10.1016/j.jss.2005.05.027
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67658910</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022480405002957</els_id><sourcerecordid>67658910</sourcerecordid><originalsourceid>FETCH-LOGICAL-c381t-ae27f9dfca4b48f5e97f28cf66b5e0748ca51e105446d03a80b3a174715880e53</originalsourceid><addsrcrecordid>eNp9kF1LHDEUhoO01NX2B3hTctPezZrM5GvwSpZWBUVRex3OZk4ky-xkm8wI_fdmugveCQdCyPMe3jyEnHG25Iyr881yk_OyZkwu56n1EVlw1srKKN18IgvG6roSholjcpLzhpV7q5sv5JgrJoUS7YL4h4QZ0yuMIQ40evoII10l3EIeMdG7Kbse6V1wKbqQ3NTvQfDz60OKfRxesKOr2Hf0aYwJXpDC0NHnBEPe9TCM_wNfyWcPfcZvh_OU_Pn963l1Xd3eX92sLm8r1xg-VoC19m3nHYi1MF5iq31tnFdqLZFpYRxIjryUF6pjDRi2boBrobk0hqFsTsnP_d5din8nzKPdhuywL0UwTtkqraRpOSsg34PlYzkn9HaXwhbSP8uZneXajS1y7SzXzlPrkvl-WD6tt9i9Jw42C_DjAEB20PviwIX8zmmpOFczd7HnsKh4DZhsdgEHh11I6EbbxfBBjTfjJZh6</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67658910</pqid></control><display><type>article</type><title>Preservation of Rat Cremaster Muscle Microcirculation after Prolonged Cold Storage and Transplantation</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Bastiaanse, Jacqueline ; Nanhekhan, Lloyd V. ; Slaaf, Dick W. ; Boeckx, Willy D. ; oude Egbrink, Mirjam G.A.</creator><creatorcontrib>Bastiaanse, Jacqueline ; Nanhekhan, Lloyd V. ; Slaaf, Dick W. ; Boeckx, Willy D. ; oude Egbrink, Mirjam G.A.</creatorcontrib><description>Microvascular surgery for the reconstruction of complex defects involves an ischemic period, which may cause flap failure as the result of ischemia/reperfusion injury. We assessed the microvascular consequences of rat cremaster muscle transplantation after prolonged periods of cold storage in HTK-Bretschneider solution (HTK). Cremaster muscle transplantations were performed immediately or after 8 or 24 h of cold storage (4°C) in HTK or saline. Intravital microscopy was used to quantify capillary perfusion and venular leukocyte-endothelium interactions following transplantation. The transplantation procedure itself resulted in 50–65 min of ischemia. After direct transplantation, capillary perfusion was 90% of control. Transplantation after 8 h of cold storage in either HTK or saline did not deteriorate capillary perfusion. When the tissue was stored for 24 h, HTK was superior to saline in preserving capillary perfusion (HTK: 76–83% of control, saline: 30%). Immediate transplantation induced a small increase in leukocyte adhesion. Prolonged cold storage in either fluid resulted in reduced flow velocities (qualitative observations) and edema formation, which hampered quantification of leukocyte-endothelium interactions. Even after 8 or 24 h of cold storage in HTK, transplantation of rat cremaster muscle was successful with good capillary perfusion. Capillary perfusion was better preserved in HTK than in saline.</description><identifier>ISSN: 0022-4804</identifier><identifier>EISSN: 1095-8673</identifier><identifier>DOI: 10.1016/j.jss.2005.05.027</identifier><identifier>PMID: 16054649</identifier><identifier>CODEN: JSGRA2</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Cell Adhesion ; Edema ; General aspects ; Glucose ; HTK ; Ischemia ; ischemia reperfusion injury ; Leukocytes ; Male ; Mannitol ; Medical sciences ; Microcirculation ; Muscle, Skeletal - blood supply ; Muscle, Skeletal - transplantation ; Potassium Chloride ; preservation ; Procaine ; Rats ; Reperfusion ; Sodium Chloride ; Specimen Handling ; Tissue Preservation - methods ; transplantation</subject><ispartof>The Journal of surgical research, 2006-03, Vol.131 (1), p.41-48</ispartof><rights>2006 Elsevier Inc.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-ae27f9dfca4b48f5e97f28cf66b5e0748ca51e105446d03a80b3a174715880e53</citedby><cites>FETCH-LOGICAL-c381t-ae27f9dfca4b48f5e97f28cf66b5e0748ca51e105446d03a80b3a174715880e53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jss.2005.05.027$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=17561169$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16054649$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bastiaanse, Jacqueline</creatorcontrib><creatorcontrib>Nanhekhan, Lloyd V.</creatorcontrib><creatorcontrib>Slaaf, Dick W.</creatorcontrib><creatorcontrib>Boeckx, Willy D.</creatorcontrib><creatorcontrib>oude Egbrink, Mirjam G.A.</creatorcontrib><title>Preservation of Rat Cremaster Muscle Microcirculation after Prolonged Cold Storage and Transplantation</title><title>The Journal of surgical research</title><addtitle>J Surg Res</addtitle><description>Microvascular surgery for the reconstruction of complex defects involves an ischemic period, which may cause flap failure as the result of ischemia/reperfusion injury. We assessed the microvascular consequences of rat cremaster muscle transplantation after prolonged periods of cold storage in HTK-Bretschneider solution (HTK). Cremaster muscle transplantations were performed immediately or after 8 or 24 h of cold storage (4°C) in HTK or saline. Intravital microscopy was used to quantify capillary perfusion and venular leukocyte-endothelium interactions following transplantation. The transplantation procedure itself resulted in 50–65 min of ischemia. After direct transplantation, capillary perfusion was 90% of control. Transplantation after 8 h of cold storage in either HTK or saline did not deteriorate capillary perfusion. When the tissue was stored for 24 h, HTK was superior to saline in preserving capillary perfusion (HTK: 76–83% of control, saline: 30%). Immediate transplantation induced a small increase in leukocyte adhesion. Prolonged cold storage in either fluid resulted in reduced flow velocities (qualitative observations) and edema formation, which hampered quantification of leukocyte-endothelium interactions. Even after 8 or 24 h of cold storage in HTK, transplantation of rat cremaster muscle was successful with good capillary perfusion. Capillary perfusion was better preserved in HTK than in saline.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Adhesion</subject><subject>Edema</subject><subject>General aspects</subject><subject>Glucose</subject><subject>HTK</subject><subject>Ischemia</subject><subject>ischemia reperfusion injury</subject><subject>Leukocytes</subject><subject>Male</subject><subject>Mannitol</subject><subject>Medical sciences</subject><subject>Microcirculation</subject><subject>Muscle, Skeletal - blood supply</subject><subject>Muscle, Skeletal - transplantation</subject><subject>Potassium Chloride</subject><subject>preservation</subject><subject>Procaine</subject><subject>Rats</subject><subject>Reperfusion</subject><subject>Sodium Chloride</subject><subject>Specimen Handling</subject><subject>Tissue Preservation - methods</subject><subject>transplantation</subject><issn>0022-4804</issn><issn>1095-8673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1LHDEUhoO01NX2B3hTctPezZrM5GvwSpZWBUVRex3OZk4ky-xkm8wI_fdmugveCQdCyPMe3jyEnHG25Iyr881yk_OyZkwu56n1EVlw1srKKN18IgvG6roSholjcpLzhpV7q5sv5JgrJoUS7YL4h4QZ0yuMIQ40evoII10l3EIeMdG7Kbse6V1wKbqQ3NTvQfDz60OKfRxesKOr2Hf0aYwJXpDC0NHnBEPe9TCM_wNfyWcPfcZvh_OU_Pn963l1Xd3eX92sLm8r1xg-VoC19m3nHYi1MF5iq31tnFdqLZFpYRxIjryUF6pjDRi2boBrobk0hqFsTsnP_d5din8nzKPdhuywL0UwTtkqraRpOSsg34PlYzkn9HaXwhbSP8uZneXajS1y7SzXzlPrkvl-WD6tt9i9Jw42C_DjAEB20PviwIX8zmmpOFczd7HnsKh4DZhsdgEHh11I6EbbxfBBjTfjJZh6</recordid><startdate>20060301</startdate><enddate>20060301</enddate><creator>Bastiaanse, Jacqueline</creator><creator>Nanhekhan, Lloyd V.</creator><creator>Slaaf, Dick W.</creator><creator>Boeckx, Willy D.</creator><creator>oude Egbrink, Mirjam G.A.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060301</creationdate><title>Preservation of Rat Cremaster Muscle Microcirculation after Prolonged Cold Storage and Transplantation</title><author>Bastiaanse, Jacqueline ; Nanhekhan, Lloyd V. ; Slaaf, Dick W. ; Boeckx, Willy D. ; oude Egbrink, Mirjam G.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-ae27f9dfca4b48f5e97f28cf66b5e0748ca51e105446d03a80b3a174715880e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Adhesion</topic><topic>Edema</topic><topic>General aspects</topic><topic>Glucose</topic><topic>HTK</topic><topic>Ischemia</topic><topic>ischemia reperfusion injury</topic><topic>Leukocytes</topic><topic>Male</topic><topic>Mannitol</topic><topic>Medical sciences</topic><topic>Microcirculation</topic><topic>Muscle, Skeletal - blood supply</topic><topic>Muscle, Skeletal - transplantation</topic><topic>Potassium Chloride</topic><topic>preservation</topic><topic>Procaine</topic><topic>Rats</topic><topic>Reperfusion</topic><topic>Sodium Chloride</topic><topic>Specimen Handling</topic><topic>Tissue Preservation - methods</topic><topic>transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bastiaanse, Jacqueline</creatorcontrib><creatorcontrib>Nanhekhan, Lloyd V.</creatorcontrib><creatorcontrib>Slaaf, Dick W.</creatorcontrib><creatorcontrib>Boeckx, Willy D.</creatorcontrib><creatorcontrib>oude Egbrink, Mirjam G.A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of surgical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bastiaanse, Jacqueline</au><au>Nanhekhan, Lloyd V.</au><au>Slaaf, Dick W.</au><au>Boeckx, Willy D.</au><au>oude Egbrink, Mirjam G.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preservation of Rat Cremaster Muscle Microcirculation after Prolonged Cold Storage and Transplantation</atitle><jtitle>The Journal of surgical research</jtitle><addtitle>J Surg Res</addtitle><date>2006-03-01</date><risdate>2006</risdate><volume>131</volume><issue>1</issue><spage>41</spage><epage>48</epage><pages>41-48</pages><issn>0022-4804</issn><eissn>1095-8673</eissn><coden>JSGRA2</coden><abstract>Microvascular surgery for the reconstruction of complex defects involves an ischemic period, which may cause flap failure as the result of ischemia/reperfusion injury. We assessed the microvascular consequences of rat cremaster muscle transplantation after prolonged periods of cold storage in HTK-Bretschneider solution (HTK). Cremaster muscle transplantations were performed immediately or after 8 or 24 h of cold storage (4°C) in HTK or saline. Intravital microscopy was used to quantify capillary perfusion and venular leukocyte-endothelium interactions following transplantation. The transplantation procedure itself resulted in 50–65 min of ischemia. After direct transplantation, capillary perfusion was 90% of control. Transplantation after 8 h of cold storage in either HTK or saline did not deteriorate capillary perfusion. When the tissue was stored for 24 h, HTK was superior to saline in preserving capillary perfusion (HTK: 76–83% of control, saline: 30%). Immediate transplantation induced a small increase in leukocyte adhesion. Prolonged cold storage in either fluid resulted in reduced flow velocities (qualitative observations) and edema formation, which hampered quantification of leukocyte-endothelium interactions. Even after 8 or 24 h of cold storage in HTK, transplantation of rat cremaster muscle was successful with good capillary perfusion. Capillary perfusion was better preserved in HTK than in saline.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>16054649</pmid><doi>10.1016/j.jss.2005.05.027</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0022-4804
ispartof The Journal of surgical research, 2006-03, Vol.131 (1), p.41-48
issn 0022-4804
1095-8673
language eng
recordid cdi_proquest_miscellaneous_67658910
source MEDLINE; Elsevier ScienceDirect Journals
subjects Animals
Biological and medical sciences
Cell Adhesion
Edema
General aspects
Glucose
HTK
Ischemia
ischemia reperfusion injury
Leukocytes
Male
Mannitol
Medical sciences
Microcirculation
Muscle, Skeletal - blood supply
Muscle, Skeletal - transplantation
Potassium Chloride
preservation
Procaine
Rats
Reperfusion
Sodium Chloride
Specimen Handling
Tissue Preservation - methods
transplantation
title Preservation of Rat Cremaster Muscle Microcirculation after Prolonged Cold Storage and Transplantation
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T06%3A57%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Preservation%20of%20Rat%20Cremaster%20Muscle%20Microcirculation%20after%20Prolonged%20Cold%20Storage%20and%20Transplantation&rft.jtitle=The%20Journal%20of%20surgical%20research&rft.au=Bastiaanse,%20Jacqueline&rft.date=2006-03-01&rft.volume=131&rft.issue=1&rft.spage=41&rft.epage=48&rft.pages=41-48&rft.issn=0022-4804&rft.eissn=1095-8673&rft.coden=JSGRA2&rft_id=info:doi/10.1016/j.jss.2005.05.027&rft_dat=%3Cproquest_cross%3E67658910%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67658910&rft_id=info:pmid/16054649&rft_els_id=S0022480405002957&rfr_iscdi=true