A Technique to Detect and to Quantify Fasciocutaneous Blood Vessels in Small Laboratory Animals Ex Vivo
A microangiographical technique is described, which allows visualization of small and capillary blood vessels and quantification of fasciocutaneous blood vessels by means of digital computer analysis in very small laboratory animals. The left carotid artery of 20 nu/nu mice was cannulated (26 gauge)...
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| Veröffentlicht in: | The Journal of surgical research 2006-03, Vol.131 (1), p.91-96 |
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| creator | Machens, H.G. Grzybowski, S. Bucsky, B. Spanholtz, T. Niedworok, C. Maichle, A. Stöckelhuber, B. Condurache, A. Liu, F. Egana, J.T. Kaun, M. Mailänder, P. Aach, T. |
| description | A microangiographical technique is described, which allows visualization of small and capillary blood vessels and quantification of fasciocutaneous blood vessels by means of digital computer analysis in very small laboratory animals.
The left carotid artery of 20 nu/nu mice was cannulated (26 gauge) and a mixture of gelatin, bariumsulfate, and green ink was injected according to standardized protocol. Fasciocutaneous blood vessels were visualized by digital mammography and analyzed for vessel length and vessel surface area as standardized units [SU] by computer program.
With the described microangiography method, fasciocutaneous blood vessels down to capillary size level can be clearly visualized. Regions of interest (ROIs) can be defined and the containing vascular network quantified. Comparable results may be obtained by calculating the microvascular area index (MAI) and the microvascular length index (MLI), related to the ROIs size. Identical ROIs showed a high reproducibility for measured [SU] < 0.01 ± 0.0012%.
Combining microsurgical techniques, pharmacological knowledge, and modern digital image technology, we were able to visualize small and capillary blood vessels even in small laboratory animals. By using our own computer analytical program, quantification of vessels was reliable, highly reproducible, and fast. |
| doi_str_mv | 10.1016/j.jss.2005.08.024 |
| format | Article |
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The left carotid artery of 20 nu/nu mice was cannulated (26 gauge) and a mixture of gelatin, bariumsulfate, and green ink was injected according to standardized protocol. Fasciocutaneous blood vessels were visualized by digital mammography and analyzed for vessel length and vessel surface area as standardized units [SU] by computer program.
With the described microangiography method, fasciocutaneous blood vessels down to capillary size level can be clearly visualized. Regions of interest (ROIs) can be defined and the containing vascular network quantified. Comparable results may be obtained by calculating the microvascular area index (MAI) and the microvascular length index (MLI), related to the ROIs size. Identical ROIs showed a high reproducibility for measured [SU] < 0.01 ± 0.0012%.
Combining microsurgical techniques, pharmacological knowledge, and modern digital image technology, we were able to visualize small and capillary blood vessels even in small laboratory animals. By using our own computer analytical program, quantification of vessels was reliable, highly reproducible, and fast.</description><identifier>ISSN: 0022-4804</identifier><identifier>EISSN: 1095-8673</identifier><identifier>DOI: 10.1016/j.jss.2005.08.024</identifier><identifier>PMID: 16274694</identifier><identifier>CODEN: JSGRA2</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>angiogenesis ; angiography ; Angiography - methods ; Angiography - veterinary ; Animals ; Animals, Laboratory ; Biological and medical sciences ; Capillaries ; Cardiovascular system ; data analysis ; Fascia - blood supply ; Female ; General aspects ; Investigative techniques, diagnostic techniques (general aspects) ; Medical sciences ; Mice ; Microcirculation ; Microsurgery - methods ; Radiodiagnosis. Nmr imagery. Nmr spectrometry ; Signal Processing, Computer-Assisted ; Skin - blood supply</subject><ispartof>The Journal of surgical research, 2006-03, Vol.131 (1), p.91-96</ispartof><rights>2006 Elsevier Inc.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-fbb34c39067e5a26fdf1effdb528b0528f6015b5bc3a2311ccf8d37ea7512f5d3</citedby><cites>FETCH-LOGICAL-c381t-fbb34c39067e5a26fdf1effdb528b0528f6015b5bc3a2311ccf8d37ea7512f5d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022480405004646$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17561177$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16274694$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Machens, H.G.</creatorcontrib><creatorcontrib>Grzybowski, S.</creatorcontrib><creatorcontrib>Bucsky, B.</creatorcontrib><creatorcontrib>Spanholtz, T.</creatorcontrib><creatorcontrib>Niedworok, C.</creatorcontrib><creatorcontrib>Maichle, A.</creatorcontrib><creatorcontrib>Stöckelhuber, B.</creatorcontrib><creatorcontrib>Condurache, A.</creatorcontrib><creatorcontrib>Liu, F.</creatorcontrib><creatorcontrib>Egana, J.T.</creatorcontrib><creatorcontrib>Kaun, M.</creatorcontrib><creatorcontrib>Mailänder, P.</creatorcontrib><creatorcontrib>Aach, T.</creatorcontrib><title>A Technique to Detect and to Quantify Fasciocutaneous Blood Vessels in Small Laboratory Animals Ex Vivo</title><title>The Journal of surgical research</title><addtitle>J Surg Res</addtitle><description>A microangiographical technique is described, which allows visualization of small and capillary blood vessels and quantification of fasciocutaneous blood vessels by means of digital computer analysis in very small laboratory animals.
The left carotid artery of 20 nu/nu mice was cannulated (26 gauge) and a mixture of gelatin, bariumsulfate, and green ink was injected according to standardized protocol. Fasciocutaneous blood vessels were visualized by digital mammography and analyzed for vessel length and vessel surface area as standardized units [SU] by computer program.
With the described microangiography method, fasciocutaneous blood vessels down to capillary size level can be clearly visualized. Regions of interest (ROIs) can be defined and the containing vascular network quantified. Comparable results may be obtained by calculating the microvascular area index (MAI) and the microvascular length index (MLI), related to the ROIs size. Identical ROIs showed a high reproducibility for measured [SU] < 0.01 ± 0.0012%.
Combining microsurgical techniques, pharmacological knowledge, and modern digital image technology, we were able to visualize small and capillary blood vessels even in small laboratory animals. By using our own computer analytical program, quantification of vessels was reliable, highly reproducible, and fast.</description><subject>angiogenesis</subject><subject>angiography</subject><subject>Angiography - methods</subject><subject>Angiography - veterinary</subject><subject>Animals</subject><subject>Animals, Laboratory</subject><subject>Biological and medical sciences</subject><subject>Capillaries</subject><subject>Cardiovascular system</subject><subject>data analysis</subject><subject>Fascia - blood supply</subject><subject>Female</subject><subject>General aspects</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Microcirculation</subject><subject>Microsurgery - methods</subject><subject>Radiodiagnosis. Nmr imagery. Nmr spectrometry</subject><subject>Signal Processing, Computer-Assisted</subject><subject>Skin - blood supply</subject><issn>0022-4804</issn><issn>1095-8673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMGOFCEURYnROO3oB7gxbHRX5QMKqI6rdpxRk06McZwtoeChdKqLEaom9t9LpzuZnRvIJee9XA4hrxm0DJh6v2t3pbQcQLbQt8C7J2TFYC2bXmnxlKwAOG-6HroL8qKUHdS81uI5uWCK606tuxX5taG36H5P8c-CdE70E87oZmonf0zfFzvNMRzojS0uJrfMdsK0FPpxTMnTOywFx0LjRH_s7TjSrR1StnPKB7qZYn0q9PovvYsP6SV5FmrEV-f7kvy8ub69-tJsv33-erXZNk70bG7CMIjOiTUojdJyFXxgGIIfJO8HqEdQwOQgBycsF4w5F3ovNFotGQ_Si0vy7rT3Pqf6pTKbfSwOx_FU3CitpOo1VJCdQJdTKRmDuc-1cT4YBuZo1-xMtWuOdg30ptqtM2_Oy5dhj_5x4qyzAm_PQPVlx5Dt5GJ55LRUjGlduQ8nrtrDh4jZVL04OfQxV_3Gp_ifGv8APheYUg</recordid><startdate>20060301</startdate><enddate>20060301</enddate><creator>Machens, H.G.</creator><creator>Grzybowski, S.</creator><creator>Bucsky, B.</creator><creator>Spanholtz, T.</creator><creator>Niedworok, C.</creator><creator>Maichle, A.</creator><creator>Stöckelhuber, B.</creator><creator>Condurache, A.</creator><creator>Liu, F.</creator><creator>Egana, J.T.</creator><creator>Kaun, M.</creator><creator>Mailänder, P.</creator><creator>Aach, T.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060301</creationdate><title>A Technique to Detect and to Quantify Fasciocutaneous Blood Vessels in Small Laboratory Animals Ex Vivo</title><author>Machens, H.G. ; Grzybowski, S. ; Bucsky, B. ; Spanholtz, T. ; Niedworok, C. ; Maichle, A. ; Stöckelhuber, B. ; Condurache, A. ; Liu, F. ; Egana, J.T. ; Kaun, M. ; Mailänder, P. ; Aach, T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-fbb34c39067e5a26fdf1effdb528b0528f6015b5bc3a2311ccf8d37ea7512f5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>angiogenesis</topic><topic>angiography</topic><topic>Angiography - methods</topic><topic>Angiography - veterinary</topic><topic>Animals</topic><topic>Animals, Laboratory</topic><topic>Biological and medical sciences</topic><topic>Capillaries</topic><topic>Cardiovascular system</topic><topic>data analysis</topic><topic>Fascia - blood supply</topic><topic>Female</topic><topic>General aspects</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Microcirculation</topic><topic>Microsurgery - methods</topic><topic>Radiodiagnosis. Nmr imagery. Nmr spectrometry</topic><topic>Signal Processing, Computer-Assisted</topic><topic>Skin - blood supply</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Machens, H.G.</creatorcontrib><creatorcontrib>Grzybowski, S.</creatorcontrib><creatorcontrib>Bucsky, B.</creatorcontrib><creatorcontrib>Spanholtz, T.</creatorcontrib><creatorcontrib>Niedworok, C.</creatorcontrib><creatorcontrib>Maichle, A.</creatorcontrib><creatorcontrib>Stöckelhuber, B.</creatorcontrib><creatorcontrib>Condurache, A.</creatorcontrib><creatorcontrib>Liu, F.</creatorcontrib><creatorcontrib>Egana, J.T.</creatorcontrib><creatorcontrib>Kaun, M.</creatorcontrib><creatorcontrib>Mailänder, P.</creatorcontrib><creatorcontrib>Aach, T.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of surgical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Machens, H.G.</au><au>Grzybowski, S.</au><au>Bucsky, B.</au><au>Spanholtz, T.</au><au>Niedworok, C.</au><au>Maichle, A.</au><au>Stöckelhuber, B.</au><au>Condurache, A.</au><au>Liu, F.</au><au>Egana, J.T.</au><au>Kaun, M.</au><au>Mailänder, P.</au><au>Aach, T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Technique to Detect and to Quantify Fasciocutaneous Blood Vessels in Small Laboratory Animals Ex Vivo</atitle><jtitle>The Journal of surgical research</jtitle><addtitle>J Surg Res</addtitle><date>2006-03-01</date><risdate>2006</risdate><volume>131</volume><issue>1</issue><spage>91</spage><epage>96</epage><pages>91-96</pages><issn>0022-4804</issn><eissn>1095-8673</eissn><coden>JSGRA2</coden><abstract>A microangiographical technique is described, which allows visualization of small and capillary blood vessels and quantification of fasciocutaneous blood vessels by means of digital computer analysis in very small laboratory animals.
The left carotid artery of 20 nu/nu mice was cannulated (26 gauge) and a mixture of gelatin, bariumsulfate, and green ink was injected according to standardized protocol. Fasciocutaneous blood vessels were visualized by digital mammography and analyzed for vessel length and vessel surface area as standardized units [SU] by computer program.
With the described microangiography method, fasciocutaneous blood vessels down to capillary size level can be clearly visualized. Regions of interest (ROIs) can be defined and the containing vascular network quantified. Comparable results may be obtained by calculating the microvascular area index (MAI) and the microvascular length index (MLI), related to the ROIs size. Identical ROIs showed a high reproducibility for measured [SU] < 0.01 ± 0.0012%.
Combining microsurgical techniques, pharmacological knowledge, and modern digital image technology, we were able to visualize small and capillary blood vessels even in small laboratory animals. By using our own computer analytical program, quantification of vessels was reliable, highly reproducible, and fast.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>16274694</pmid><doi>10.1016/j.jss.2005.08.024</doi><tpages>6</tpages></addata></record> |
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| subjects | angiogenesis angiography Angiography - methods Angiography - veterinary Animals Animals, Laboratory Biological and medical sciences Capillaries Cardiovascular system data analysis Fascia - blood supply Female General aspects Investigative techniques, diagnostic techniques (general aspects) Medical sciences Mice Microcirculation Microsurgery - methods Radiodiagnosis. Nmr imagery. Nmr spectrometry Signal Processing, Computer-Assisted Skin - blood supply |
| title | A Technique to Detect and to Quantify Fasciocutaneous Blood Vessels in Small Laboratory Animals Ex Vivo |
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