Enhanced Resistance to Botrytis cinerea Mediated by the Transgenic Expression of the Chitinase Gene ch5B in Strawberry
Plants of strawberry (cultivar Pájaro) were transformed with three defense related genes: ch5B, gln2 and ap24 using Agrobacterium tumefaciens. The ch5B gene encodes for a chitinase from Phaseolus vulgaris, while gln2 and ap24 encode for a glucanase and a thaumatin-like protein, respectively, both fr...
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| Veröffentlicht in: | Transgenic research 2006-02, Vol.15 (1), p.57-68 |
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| creator | Vellicce, G.R Diaz Ricci, J.C Hernandez, L Castagnaro, A.P |
| description | Plants of strawberry (cultivar Pájaro) were transformed with three defense related genes: ch5B, gln2 and ap24 using Agrobacterium tumefaciens. The ch5B gene encodes for a chitinase from Phaseolus vulgaris, while gln2 and ap24 encode for a glucanase and a thaumatin-like protein, respectively, both from Nicotiana tabacum. Sixteen transgenic lines expressing one or a combination of two defense genes were obtained. Phytopathological tests showed that two transgenic lines expressing only the ch5B gene displayed high levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward Colletotrichum acutatum, the etiological agent of the anthracnose disease, was not enhanced in the transgenic plants. These results suggest that the ch5B gene can be used to introduce transgene-mediated resistance to gray mold in strawberry, due to the lack of natural resistance to this disease in the crop. |
| doi_str_mv | 10.1007/s11248-005-2543-6 |
| format | Article |
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The ch5B gene encodes for a chitinase from Phaseolus vulgaris, while gln2 and ap24 encode for a glucanase and a thaumatin-like protein, respectively, both from Nicotiana tabacum. Sixteen transgenic lines expressing one or a combination of two defense genes were obtained. Phytopathological tests showed that two transgenic lines expressing only the ch5B gene displayed high levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward Colletotrichum acutatum, the etiological agent of the anthracnose disease, was not enhanced in the transgenic plants. These results suggest that the ch5B gene can be used to introduce transgene-mediated resistance to gray mold in strawberry, due to the lack of natural resistance to this disease in the crop.</description><identifier>ISSN: 0962-8819</identifier><identifier>EISSN: 1573-9368</identifier><identifier>DOI: 10.1007/s11248-005-2543-6</identifier><identifier>PMID: 16475010</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Agrobacterium tumefaciens ; anthracnose ; beta-glucanase ; Biological and medical sciences ; Biotechnology ; Blotting, Northern ; Botrytis ; Botrytis cinerea ; ch5B gene ; Chitinase ; Chitinases - biosynthesis ; Chitinases - genetics ; Chitinases - physiology ; Colletotrichum acutatum ; disease resistance ; Fabaceae - enzymology ; Fabaceae - genetics ; foliar diseases ; Fragaria ; Fragaria - enzymology ; Fragaria - genetics ; Fragaria - microbiology ; Fragaria ananassa ; Fundamental and applied biological sciences. Psychology ; fungal diseases of plants ; gene expression ; Genes ; Genetic engineering ; Genetic technics ; genetic transformation ; leaves ; Methods. Procedures. Technologies ; Nicotiana tabacum ; pathogenesis-related proteins ; Phaseolus vulgaris ; Plant Diseases - genetics ; Plant Diseases - microbiology ; Plants, Genetically Modified ; strawberries ; thaumatin-like protein ; transgenes ; Transgenic animals and transgenic plants ; Transgenic plants</subject><ispartof>Transgenic research, 2006-02, Vol.15 (1), p.57-68</ispartof><rights>2006 INIST-CNRS</rights><rights>Springer 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-e1c1ee17a8786b1b235af7ee2035a3ccc239437de59bc0a62d31ede08c3b83193</citedby><cites>FETCH-LOGICAL-c411t-e1c1ee17a8786b1b235af7ee2035a3ccc239437de59bc0a62d31ede08c3b83193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17538058$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16475010$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vellicce, G.R</creatorcontrib><creatorcontrib>Diaz Ricci, J.C</creatorcontrib><creatorcontrib>Hernandez, L</creatorcontrib><creatorcontrib>Castagnaro, A.P</creatorcontrib><title>Enhanced Resistance to Botrytis cinerea Mediated by the Transgenic Expression of the Chitinase Gene ch5B in Strawberry</title><title>Transgenic research</title><addtitle>Transgenic Res</addtitle><description>Plants of strawberry (cultivar Pájaro) were transformed with three defense related genes: ch5B, gln2 and ap24 using Agrobacterium tumefaciens. The ch5B gene encodes for a chitinase from Phaseolus vulgaris, while gln2 and ap24 encode for a glucanase and a thaumatin-like protein, respectively, both from Nicotiana tabacum. Sixteen transgenic lines expressing one or a combination of two defense genes were obtained. Phytopathological tests showed that two transgenic lines expressing only the ch5B gene displayed high levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward Colletotrichum acutatum, the etiological agent of the anthracnose disease, was not enhanced in the transgenic plants. These results suggest that the ch5B gene can be used to introduce transgene-mediated resistance to gray mold in strawberry, due to the lack of natural resistance to this disease in the crop.</description><subject>Agrobacterium tumefaciens</subject><subject>anthracnose</subject><subject>beta-glucanase</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Blotting, Northern</subject><subject>Botrytis</subject><subject>Botrytis cinerea</subject><subject>ch5B gene</subject><subject>Chitinase</subject><subject>Chitinases - biosynthesis</subject><subject>Chitinases - genetics</subject><subject>Chitinases - physiology</subject><subject>Colletotrichum acutatum</subject><subject>disease resistance</subject><subject>Fabaceae - enzymology</subject><subject>Fabaceae - genetics</subject><subject>foliar diseases</subject><subject>Fragaria</subject><subject>Fragaria - enzymology</subject><subject>Fragaria - genetics</subject><subject>Fragaria - microbiology</subject><subject>Fragaria ananassa</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>fungal diseases of plants</subject><subject>gene expression</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>genetic transformation</subject><subject>leaves</subject><subject>Methods. Procedures. Technologies</subject><subject>Nicotiana tabacum</subject><subject>pathogenesis-related proteins</subject><subject>Phaseolus vulgaris</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - microbiology</subject><subject>Plants, Genetically Modified</subject><subject>strawberries</subject><subject>thaumatin-like protein</subject><subject>transgenes</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenic plants</subject><issn>0962-8819</issn><issn>1573-9368</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkc1uEzEURi0EoiHwAGzAQoLdgK89_pllG4WCVIRE27Xl8dxpXCWeYDtA3h6niVSJDStfy-f7rqxDyGtgH4Ex_SkD8NY0jMmGy1Y06gmZgdSi6YQyT8mMdYo3xkB3Rl7kfM9YTRnxnJyBarWstxn5tYwrFz0O9AfmkMthpmWiF1NJ-xIy9SFiQke_4RBcqVy_p2WF9Ca5mO8wBk-Xf7YJcw5TpNP48LhYhRKiy0gvMSL1K3lBQ6TXJbnfPaa0f0mejW6d8dXpnJPbz8ubxZfm6vvl18X5VeNbgNIgeEAE7Yw2qoeeC-lGjchZHYT3nouuFXpA2fWeOcUHATggM170RkAn5uTDsXebpp87zMVuQva4XruI0y5bpZVUhvP_gtAZYboH8N0_4P20S7F-wmrJqwpWyTmBI-TTlHPC0W5T2Li0t8DsQZ09qrOVtwd1VtXMm1Pxrt_g8Jg4uarA-xPgsnfrsQrwIT9yWgrD5GH52yM3usm6u1SZ22vOQNQSJQFa8RdnJan6</recordid><startdate>20060201</startdate><enddate>20060201</enddate><creator>Vellicce, G.R</creator><creator>Diaz Ricci, J.C</creator><creator>Hernandez, L</creator><creator>Castagnaro, A.P</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7QL</scope><scope>7QO</scope><scope>C1K</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20060201</creationdate><title>Enhanced Resistance to Botrytis cinerea Mediated by the Transgenic Expression of the Chitinase Gene ch5B in Strawberry</title><author>Vellicce, G.R ; Diaz Ricci, J.C ; Hernandez, L ; Castagnaro, A.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-e1c1ee17a8786b1b235af7ee2035a3ccc239437de59bc0a62d31ede08c3b83193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Agrobacterium tumefaciens</topic><topic>anthracnose</topic><topic>beta-glucanase</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Blotting, Northern</topic><topic>Botrytis</topic><topic>Botrytis cinerea</topic><topic>ch5B gene</topic><topic>Chitinase</topic><topic>Chitinases - biosynthesis</topic><topic>Chitinases - genetics</topic><topic>Chitinases - physiology</topic><topic>Colletotrichum acutatum</topic><topic>disease resistance</topic><topic>Fabaceae - enzymology</topic><topic>Fabaceae - genetics</topic><topic>foliar diseases</topic><topic>Fragaria</topic><topic>Fragaria - enzymology</topic><topic>Fragaria - genetics</topic><topic>Fragaria - microbiology</topic><topic>Fragaria ananassa</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>fungal diseases of plants</topic><topic>gene expression</topic><topic>Genes</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>genetic transformation</topic><topic>leaves</topic><topic>Methods. Procedures. Technologies</topic><topic>Nicotiana tabacum</topic><topic>pathogenesis-related proteins</topic><topic>Phaseolus vulgaris</topic><topic>Plant Diseases - genetics</topic><topic>Plant Diseases - microbiology</topic><topic>Plants, Genetically Modified</topic><topic>strawberries</topic><topic>thaumatin-like protein</topic><topic>transgenes</topic><topic>Transgenic animals and transgenic plants</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vellicce, G.R</creatorcontrib><creatorcontrib>Diaz Ricci, J.C</creatorcontrib><creatorcontrib>Hernandez, L</creatorcontrib><creatorcontrib>Castagnaro, A.P</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Transgenic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vellicce, G.R</au><au>Diaz Ricci, J.C</au><au>Hernandez, L</au><au>Castagnaro, A.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced Resistance to Botrytis cinerea Mediated by the Transgenic Expression of the Chitinase Gene ch5B in Strawberry</atitle><jtitle>Transgenic research</jtitle><addtitle>Transgenic Res</addtitle><date>2006-02-01</date><risdate>2006</risdate><volume>15</volume><issue>1</issue><spage>57</spage><epage>68</epage><pages>57-68</pages><issn>0962-8819</issn><eissn>1573-9368</eissn><abstract>Plants of strawberry (cultivar Pájaro) were transformed with three defense related genes: ch5B, gln2 and ap24 using Agrobacterium tumefaciens. The ch5B gene encodes for a chitinase from Phaseolus vulgaris, while gln2 and ap24 encode for a glucanase and a thaumatin-like protein, respectively, both from Nicotiana tabacum. Sixteen transgenic lines expressing one or a combination of two defense genes were obtained. Phytopathological tests showed that two transgenic lines expressing only the ch5B gene displayed high levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward Colletotrichum acutatum, the etiological agent of the anthracnose disease, was not enhanced in the transgenic plants. These results suggest that the ch5B gene can be used to introduce transgene-mediated resistance to gray mold in strawberry, due to the lack of natural resistance to this disease in the crop.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>16475010</pmid><doi>10.1007/s11248-005-2543-6</doi><tpages>12</tpages></addata></record> |
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| subjects | Agrobacterium tumefaciens anthracnose beta-glucanase Biological and medical sciences Biotechnology Blotting, Northern Botrytis Botrytis cinerea ch5B gene Chitinase Chitinases - biosynthesis Chitinases - genetics Chitinases - physiology Colletotrichum acutatum disease resistance Fabaceae - enzymology Fabaceae - genetics foliar diseases Fragaria Fragaria - enzymology Fragaria - genetics Fragaria - microbiology Fragaria ananassa Fundamental and applied biological sciences. Psychology fungal diseases of plants gene expression Genes Genetic engineering Genetic technics genetic transformation leaves Methods. Procedures. Technologies Nicotiana tabacum pathogenesis-related proteins Phaseolus vulgaris Plant Diseases - genetics Plant Diseases - microbiology Plants, Genetically Modified strawberries thaumatin-like protein transgenes Transgenic animals and transgenic plants Transgenic plants |
| title | Enhanced Resistance to Botrytis cinerea Mediated by the Transgenic Expression of the Chitinase Gene ch5B in Strawberry |
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