Bovine monocytes and a macrophage cell line differ in their ability to phagocytose and support the intracellular survival of Mycobacterium avium subsp. paratuberculosis
Bovine monocytes exhibited a greater ability to phagocytose Mycobacterium avium subsp. paratuberculosis (i.e. greater percentage of infected cells, and more bacilli per infected cell), than did a bovine macrophage cell line (BoMac). Phagocytosis of M. paratuberculosis by monocytes, but not the cell...
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| Veröffentlicht in: | Veterinary Immunology and Immunopathology 2006-03, Vol.110 (1), p.109-120 |
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| creator | Woo, Seng-Ryong Sotos, Josh Hart, Arlene P. Barletta, Raúl G. Czuprynski, Charles J. |
| description | Bovine monocytes exhibited a greater ability to phagocytose
Mycobacterium avium subsp.
paratuberculosis (i.e. greater percentage of infected cells, and more bacilli per infected cell), than did a bovine macrophage cell line (BoMac). Phagocytosis of
M. paratuberculosis by monocytes, but not the cell line, was significantly enhanced by the addition of autologous serum. Following ingestion, the numbers of viable
M. paratuberculosis cells in monocytes increased during the first 4 days and then declined between day 4 and day 8 after infection, as determined by a radiometric method. In contrast, BoMac cells were not permissive for bacillary multiplication; the numbers of
M. paratuberculosis remained largely unchanged in the cell line during the 8 day incubation period. The numbers of microscopically visible acid-fast bacilli increased with time in monocytes but not in the macrophage cell line. These observations suggest that replication and inactivation of bacilli may both occur in monocytes. The differing abilities of bovine monocytes and the macrophage cell line to ingest and restrain the intracellular growth of
M. paratuberculosis provide contrasting model systems for investigating how
M. paratuberculosis enters and persists within its preferred niche, the mononuclear phagocyte. |
| doi_str_mv | 10.1016/j.vetimm.2005.09.010 |
| format | Article |
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Mycobacterium avium subsp.
paratuberculosis (i.e. greater percentage of infected cells, and more bacilli per infected cell), than did a bovine macrophage cell line (BoMac). Phagocytosis of
M. paratuberculosis by monocytes, but not the cell line, was significantly enhanced by the addition of autologous serum. Following ingestion, the numbers of viable
M. paratuberculosis cells in monocytes increased during the first 4 days and then declined between day 4 and day 8 after infection, as determined by a radiometric method. In contrast, BoMac cells were not permissive for bacillary multiplication; the numbers of
M. paratuberculosis remained largely unchanged in the cell line during the 8 day incubation period. The numbers of microscopically visible acid-fast bacilli increased with time in monocytes but not in the macrophage cell line. These observations suggest that replication and inactivation of bacilli may both occur in monocytes. The differing abilities of bovine monocytes and the macrophage cell line to ingest and restrain the intracellular growth of
M. paratuberculosis provide contrasting model systems for investigating how
M. paratuberculosis enters and persists within its preferred niche, the mononuclear phagocyte.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>EISSN: 1365-2567</identifier><identifier>DOI: 10.1016/j.vetimm.2005.09.010</identifier><identifier>PMID: 16256206</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; blood serum ; Bovine ; Cattle ; cattle diseases ; Cattle Diseases - immunology ; Cattle Diseases - microbiology ; CD11 Antigens - immunology ; CD11b Antigen - immunology ; cell invasion ; Cell Line ; cell lines ; Cell Survival - immunology ; food animals ; Intracellular ; Macrophage ; macrophages ; Macrophages, Peritoneal - immunology ; Macrophages, Peritoneal - microbiology ; microbial growth ; Microspheres ; Monocyte ; monocytes ; Monocytes - immunology ; Monocytes - microbiology ; Mycobacterium avium ; Mycobacterium avium subsp. paratuberculosis ; Mycobacterium avium subsp. paratuberculosis - immunology ; Mycobacterium paratuberculosis ; paratuberculosis ; Paratuberculosis - immunology ; Paratuberculosis - microbiology ; phagocytes ; Phagocytosis ; Phagocytosis - immunology ; Zymosan - immunology</subject><ispartof>Veterinary Immunology and Immunopathology, 2006-03, Vol.110 (1), p.109-120</ispartof><rights>2005 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-4b3da56179705b5f28b996ad2ddc36154f98dc91223c79b79e47a66fcf4197e53</citedby><cites>FETCH-LOGICAL-c415t-4b3da56179705b5f28b996ad2ddc36154f98dc91223c79b79e47a66fcf4197e53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0165242705002928$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16256206$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Woo, Seng-Ryong</creatorcontrib><creatorcontrib>Sotos, Josh</creatorcontrib><creatorcontrib>Hart, Arlene P.</creatorcontrib><creatorcontrib>Barletta, Raúl G.</creatorcontrib><creatorcontrib>Czuprynski, Charles J.</creatorcontrib><title>Bovine monocytes and a macrophage cell line differ in their ability to phagocytose and support the intracellular survival of Mycobacterium avium subsp. paratuberculosis</title><title>Veterinary Immunology and Immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>Bovine monocytes exhibited a greater ability to phagocytose
Mycobacterium avium subsp.
paratuberculosis (i.e. greater percentage of infected cells, and more bacilli per infected cell), than did a bovine macrophage cell line (BoMac). Phagocytosis of
M. paratuberculosis by monocytes, but not the cell line, was significantly enhanced by the addition of autologous serum. Following ingestion, the numbers of viable
M. paratuberculosis cells in monocytes increased during the first 4 days and then declined between day 4 and day 8 after infection, as determined by a radiometric method. In contrast, BoMac cells were not permissive for bacillary multiplication; the numbers of
M. paratuberculosis remained largely unchanged in the cell line during the 8 day incubation period. The numbers of microscopically visible acid-fast bacilli increased with time in monocytes but not in the macrophage cell line. These observations suggest that replication and inactivation of bacilli may both occur in monocytes. The differing abilities of bovine monocytes and the macrophage cell line to ingest and restrain the intracellular growth of
M. paratuberculosis provide contrasting model systems for investigating how
M. paratuberculosis enters and persists within its preferred niche, the mononuclear phagocyte.</description><subject>Animals</subject><subject>blood serum</subject><subject>Bovine</subject><subject>Cattle</subject><subject>cattle diseases</subject><subject>Cattle Diseases - immunology</subject><subject>Cattle Diseases - microbiology</subject><subject>CD11 Antigens - immunology</subject><subject>CD11b Antigen - immunology</subject><subject>cell invasion</subject><subject>Cell Line</subject><subject>cell lines</subject><subject>Cell Survival - immunology</subject><subject>food animals</subject><subject>Intracellular</subject><subject>Macrophage</subject><subject>macrophages</subject><subject>Macrophages, Peritoneal - immunology</subject><subject>Macrophages, Peritoneal - microbiology</subject><subject>microbial growth</subject><subject>Microspheres</subject><subject>Monocyte</subject><subject>monocytes</subject><subject>Monocytes - immunology</subject><subject>Monocytes - microbiology</subject><subject>Mycobacterium avium</subject><subject>Mycobacterium avium subsp. paratuberculosis</subject><subject>Mycobacterium avium subsp. paratuberculosis - immunology</subject><subject>Mycobacterium paratuberculosis</subject><subject>paratuberculosis</subject><subject>Paratuberculosis - immunology</subject><subject>Paratuberculosis - microbiology</subject><subject>phagocytes</subject><subject>Phagocytosis</subject><subject>Phagocytosis - immunology</subject><subject>Zymosan - immunology</subject><issn>0165-2427</issn><issn>1873-2534</issn><issn>1365-2567</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0cGO1SAUBuDGaJzr6BsYZeWuFWiBsjFxJjqajHGhsyYUTme4aUsF2uS-kY8ptTeZnW4OCz7-nPAXxWuCK4IJf3-sVkhuHCuKMauwrDDBT4oDaUVdUlY3T4tDZqykDRUXxYsYjzhD2bbPiwvCKeMU80Px-8qvbgI0-smbU4KI9GSRRqM2wc8P-h6QgWFAw4as63sIyE0oPYALSHducOmEkkcb3QJ8hL8JcZlnH9IGs09BbynLoEO-Catb9YB8j76djO-0SRDcMiK9bjMuXZwrNOug09JBMMvgo4svi2e9HiK8Op-Xxd3nTz-vv5S332--Xn-8LU1DWCqbrraacSKkwKxjPW07Kbm21FpTc8KaXrbWSEJpbYTshIRGaM570zdECmD1ZfFuz52D_7VATGp0cVteT-CXqLjgjHPe_BcSgWXNKcmw2WH-0RgD9GoObtThpAhWW5XqqPYq1ValwlLlKvOzN-f8pRvBPj46d5fB2x302it9H1xUdz8oJjUmJA9Bs_iwC8gftjoIKhoHkwHrApikrHf_3uEPMGK_Kg</recordid><startdate>20060315</startdate><enddate>20060315</enddate><creator>Woo, Seng-Ryong</creator><creator>Sotos, Josh</creator><creator>Hart, Arlene P.</creator><creator>Barletta, Raúl G.</creator><creator>Czuprynski, Charles J.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20060315</creationdate><title>Bovine monocytes and a macrophage cell line differ in their ability to phagocytose and support the intracellular survival of Mycobacterium avium subsp. paratuberculosis</title><author>Woo, Seng-Ryong ; Sotos, Josh ; Hart, Arlene P. ; Barletta, Raúl G. ; Czuprynski, Charles J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-4b3da56179705b5f28b996ad2ddc36154f98dc91223c79b79e47a66fcf4197e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>blood serum</topic><topic>Bovine</topic><topic>Cattle</topic><topic>cattle diseases</topic><topic>Cattle Diseases - immunology</topic><topic>Cattle Diseases - microbiology</topic><topic>CD11 Antigens - immunology</topic><topic>CD11b Antigen - immunology</topic><topic>cell invasion</topic><topic>Cell Line</topic><topic>cell lines</topic><topic>Cell Survival - immunology</topic><topic>food animals</topic><topic>Intracellular</topic><topic>Macrophage</topic><topic>macrophages</topic><topic>Macrophages, Peritoneal - immunology</topic><topic>Macrophages, Peritoneal - microbiology</topic><topic>microbial growth</topic><topic>Microspheres</topic><topic>Monocyte</topic><topic>monocytes</topic><topic>Monocytes - immunology</topic><topic>Monocytes - microbiology</topic><topic>Mycobacterium avium</topic><topic>Mycobacterium avium subsp. paratuberculosis</topic><topic>Mycobacterium avium subsp. paratuberculosis - immunology</topic><topic>Mycobacterium paratuberculosis</topic><topic>paratuberculosis</topic><topic>Paratuberculosis - immunology</topic><topic>Paratuberculosis - microbiology</topic><topic>phagocytes</topic><topic>Phagocytosis</topic><topic>Phagocytosis - immunology</topic><topic>Zymosan - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Woo, Seng-Ryong</creatorcontrib><creatorcontrib>Sotos, Josh</creatorcontrib><creatorcontrib>Hart, Arlene P.</creatorcontrib><creatorcontrib>Barletta, Raúl G.</creatorcontrib><creatorcontrib>Czuprynski, Charles J.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary Immunology and Immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Woo, Seng-Ryong</au><au>Sotos, Josh</au><au>Hart, Arlene P.</au><au>Barletta, Raúl G.</au><au>Czuprynski, Charles J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bovine monocytes and a macrophage cell line differ in their ability to phagocytose and support the intracellular survival of Mycobacterium avium subsp. paratuberculosis</atitle><jtitle>Veterinary Immunology and Immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>2006-03-15</date><risdate>2006</risdate><volume>110</volume><issue>1</issue><spage>109</spage><epage>120</epage><pages>109-120</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><eissn>1365-2567</eissn><abstract>Bovine monocytes exhibited a greater ability to phagocytose
Mycobacterium avium subsp.
paratuberculosis (i.e. greater percentage of infected cells, and more bacilli per infected cell), than did a bovine macrophage cell line (BoMac). Phagocytosis of
M. paratuberculosis by monocytes, but not the cell line, was significantly enhanced by the addition of autologous serum. Following ingestion, the numbers of viable
M. paratuberculosis cells in monocytes increased during the first 4 days and then declined between day 4 and day 8 after infection, as determined by a radiometric method. In contrast, BoMac cells were not permissive for bacillary multiplication; the numbers of
M. paratuberculosis remained largely unchanged in the cell line during the 8 day incubation period. The numbers of microscopically visible acid-fast bacilli increased with time in monocytes but not in the macrophage cell line. These observations suggest that replication and inactivation of bacilli may both occur in monocytes. The differing abilities of bovine monocytes and the macrophage cell line to ingest and restrain the intracellular growth of
M. paratuberculosis provide contrasting model systems for investigating how
M. paratuberculosis enters and persists within its preferred niche, the mononuclear phagocyte.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>16256206</pmid><doi>10.1016/j.vetimm.2005.09.010</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-2427 |
| ispartof | Veterinary Immunology and Immunopathology, 2006-03, Vol.110 (1), p.109-120 |
| issn | 0165-2427 1873-2534 1365-2567 |
| language | eng |
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| source | Wiley Free Content; MEDLINE; IngentaConnect Free/Open Access Journals; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Animals blood serum Bovine Cattle cattle diseases Cattle Diseases - immunology Cattle Diseases - microbiology CD11 Antigens - immunology CD11b Antigen - immunology cell invasion Cell Line cell lines Cell Survival - immunology food animals Intracellular Macrophage macrophages Macrophages, Peritoneal - immunology Macrophages, Peritoneal - microbiology microbial growth Microspheres Monocyte monocytes Monocytes - immunology Monocytes - microbiology Mycobacterium avium Mycobacterium avium subsp. paratuberculosis Mycobacterium avium subsp. paratuberculosis - immunology Mycobacterium paratuberculosis paratuberculosis Paratuberculosis - immunology Paratuberculosis - microbiology phagocytes Phagocytosis Phagocytosis - immunology Zymosan - immunology |
| title | Bovine monocytes and a macrophage cell line differ in their ability to phagocytose and support the intracellular survival of Mycobacterium avium subsp. paratuberculosis |
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