Use of a FLAER-Based WBC Assay in the Primary Screening of PNH Clones
Diagnosis of paroxysmal nocturnal hemoglobinuria (PNH) with flow cytometry traditionally involves the analysis of CD55 and CD59 on RBCs and neutrophils. However, the ability to accurately detect PNH RBCs is compromised by prior hemolysis and/or transfused RBCs. Patients with aplastic anemia (AA) and...
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| Veröffentlicht in: | American journal of clinical pathology 2009-10, Vol.132 (4), p.564-572 |
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| container_title | American journal of clinical pathology |
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| creator | SUTHERLAND, D. Robert KUEK, Nancy AZCONA-OLIVERA, Juan ANDERSON, Tanya ACTON, Erica BARTH, David KEENEY, Michael |
| description | Diagnosis of paroxysmal nocturnal hemoglobinuria (PNH) with flow cytometry traditionally involves the analysis of CD55 and CD59 on RBCs and neutrophils. However, the ability to accurately detect PNH RBCs is compromised by prior hemolysis and/or transfused RBCs. Patients with aplastic anemia (AA) and myelodysplastic syndrome (MDS) can also produce PNH clones. We recently described a multiparameter fluorescent aerolysin (FLAER)-based flow assay using CD45, CD33, and CD14 that accurately identified PNH monocyte and neutrophil clones in PNH, AA, and MDS. Here, we compared the efficiency of this WBC assay with a CD59-based assay on RBCs during a 3-year period. PNH clones were detected with the FLAER assay in 63 (11.8%) of 536 samples tested, whereas PNH RBCs were detected in only 33 (6.2%), and always with a smaller clone size. The FLAER assay on WBCs is a more sensitive and robust primary screening assay for detecting PNH clones in clinical samples. |
| doi_str_mv | 10.1309/ajcpmrdzzfqm7yj4 |
| format | Article |
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PNH clones were detected with the FLAER assay in 63 (11.8%) of 536 samples tested, whereas PNH RBCs were detected in only 33 (6.2%), and always with a smaller clone size. The FLAER assay on WBCs is a more sensitive and robust primary screening assay for detecting PNH clones in clinical samples.</description><identifier>ISSN: 0002-9173</identifier><identifier>EISSN: 1943-7722</identifier><identifier>DOI: 10.1309/ajcpmrdzzfqm7yj4</identifier><identifier>PMID: 19762534</identifier><identifier>CODEN: AJCPAI</identifier><language>eng</language><publisher>Chicago, IL: American Society of Clinical Pathologists</publisher><subject>Anemia, Aplastic - immunology ; Anemias. 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We recently described a multiparameter fluorescent aerolysin (FLAER)-based flow assay using CD45, CD33, and CD14 that accurately identified PNH monocyte and neutrophil clones in PNH, AA, and MDS. Here, we compared the efficiency of this WBC assay with a CD59-based assay on RBCs during a 3-year period. PNH clones were detected with the FLAER assay in 63 (11.8%) of 536 samples tested, whereas PNH RBCs were detected in only 33 (6.2%), and always with a smaller clone size. The FLAER assay on WBCs is a more sensitive and robust primary screening assay for detecting PNH clones in clinical samples.</description><subject>Anemia, Aplastic - immunology</subject><subject>Anemias. Hemoglobinopathies</subject><subject>Bacterial Toxins</subject><subject>Biological and medical sciences</subject><subject>CD59 Antigens - analysis</subject><subject>Clone Cells</subject><subject>Diseases of red blood cells</subject><subject>Erythrocyte Count - methods</subject><subject>Flow Cytometry</subject><subject>Fluorescein-5-isothiocyanate</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Hemoglobinuria, Paroxysmal - diagnosis</subject><subject>Hemoglobinuria, Paroxysmal - immunology</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Leukocyte Count - methods</subject><subject>Medical sciences</subject><subject>Myelodysplastic Syndromes - immunology</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Pore Forming Cytotoxic Proteins</subject><subject>Sensitivity and Specificity</subject><issn>0002-9173</issn><issn>1943-7722</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM9PwjAYhhujEUTvnkwvepu2XbeuR5ggElBEiZHL0nbfdGQ_YIUD_PWOsOjpuzzvk-99Ebqm5J66RD6opVnlVbzfJ-tc7Jb8BLWp5K4jBGOnqE0IYY6kwm2hC2uXhFAWEH6OWlQKn3kub6P-3AIuE6zwYNztz5yeshDjz16Iu9aqHU4LvPkBPK3SXFU7_G4qgCItvg-Z6csQh1lZgL1EZ4nKLFw1t4Pmg_5HOHTGr0_PYXfsGO56Gwfqvwzz4iQgSkvDlB8owX2qgRqtPWCC6kAaIyDQQISW4HMlRJBQJYiui3XQ3dG7qsr1FuwmylNrIMtUAeXWRr7wPd9nsgbJETRVaW0FSbQ6NogoiQ7TRd1ROJ3MHheLwdtEfI0O7pvGvdU5xP-BZqsauG0AZY3KkkoVJrV_HKPS4wEX7i9yDHdp</recordid><startdate>20091001</startdate><enddate>20091001</enddate><creator>SUTHERLAND, D. 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Miscellaneous investigative techniques</topic><topic>Pore Forming Cytotoxic Proteins</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SUTHERLAND, D. Robert</creatorcontrib><creatorcontrib>KUEK, Nancy</creatorcontrib><creatorcontrib>AZCONA-OLIVERA, Juan</creatorcontrib><creatorcontrib>ANDERSON, Tanya</creatorcontrib><creatorcontrib>ACTON, Erica</creatorcontrib><creatorcontrib>BARTH, David</creatorcontrib><creatorcontrib>KEENEY, Michael</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SUTHERLAND, D. Robert</au><au>KUEK, Nancy</au><au>AZCONA-OLIVERA, Juan</au><au>ANDERSON, Tanya</au><au>ACTON, Erica</au><au>BARTH, David</au><au>KEENEY, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of a FLAER-Based WBC Assay in the Primary Screening of PNH Clones</atitle><jtitle>American journal of clinical pathology</jtitle><addtitle>Am J Clin Pathol</addtitle><date>2009-10-01</date><risdate>2009</risdate><volume>132</volume><issue>4</issue><spage>564</spage><epage>572</epage><pages>564-572</pages><issn>0002-9173</issn><eissn>1943-7722</eissn><coden>AJCPAI</coden><abstract>Diagnosis of paroxysmal nocturnal hemoglobinuria (PNH) with flow cytometry traditionally involves the analysis of CD55 and CD59 on RBCs and neutrophils. However, the ability to accurately detect PNH RBCs is compromised by prior hemolysis and/or transfused RBCs. Patients with aplastic anemia (AA) and myelodysplastic syndrome (MDS) can also produce PNH clones. We recently described a multiparameter fluorescent aerolysin (FLAER)-based flow assay using CD45, CD33, and CD14 that accurately identified PNH monocyte and neutrophil clones in PNH, AA, and MDS. Here, we compared the efficiency of this WBC assay with a CD59-based assay on RBCs during a 3-year period. PNH clones were detected with the FLAER assay in 63 (11.8%) of 536 samples tested, whereas PNH RBCs were detected in only 33 (6.2%), and always with a smaller clone size. The FLAER assay on WBCs is a more sensitive and robust primary screening assay for detecting PNH clones in clinical samples.</abstract><cop>Chicago, IL</cop><pub>American Society of Clinical Pathologists</pub><pmid>19762534</pmid><doi>10.1309/ajcpmrdzzfqm7yj4</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Anemia, Aplastic - immunology Anemias. Hemoglobinopathies Bacterial Toxins Biological and medical sciences CD59 Antigens - analysis Clone Cells Diseases of red blood cells Erythrocyte Count - methods Flow Cytometry Fluorescein-5-isothiocyanate Hematologic and hematopoietic diseases Hemoglobinuria, Paroxysmal - diagnosis Hemoglobinuria, Paroxysmal - immunology Humans Investigative techniques, diagnostic techniques (general aspects) Leukocyte Count - methods Medical sciences Myelodysplastic Syndromes - immunology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Pore Forming Cytotoxic Proteins Sensitivity and Specificity |
| title | Use of a FLAER-Based WBC Assay in the Primary Screening of PNH Clones |
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