Live attenuated vaccine-based control of necrotic enteritis of broiler chickens
A vaccine for necrotic enteritis (NE) of chickens would reduce the current need to prevent or treat the disease in broiler chickens with antimicrobial drugs. The objective of this study was to understand aspects of immunity to the disease. The first experiment examined the virulence of six strains o...
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| Veröffentlicht in: | Veterinary microbiology 2006-03, Vol.113 (1), p.25-34 |
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| creator | Thompson, D.R. Parreira, V.R. Kulkarni, R.R. Prescott, J.F. |
| description | A vaccine for necrotic enteritis (NE) of chickens would reduce the current need to prevent or treat the disease in broiler chickens with antimicrobial drugs. The objective of this study was to understand aspects of immunity to the disease. The first experiment examined the virulence of six strains of
Clostridium perfringens isolated from cases of NE in broiler chickens. Using a 5-day experimental oral infection of 2-week-old broiler chickens, four of the six strains were found to be virulent. Pulsed-field gel electrophoresis and PCR showed that virulence was not associated with a plasmid encoding the beta2 toxin gene,
cpb2, since this was present in virulent and one of the two avirulent strains.
In the second experiment, two virulent and one avirulent strains were tested for their ability to immunize (“infection-immunization”) chickens through the oral route. The procedure used experimental infection for 5 days followed by bacitracin treatment for 9 days, and then re-challenge 2 days later with a virulent strain, CP4. Infection-immunization with the virulent isolates protected chickens from subsequent virulent challenge, whereas the infection-immunization with the avirulent isolate did not. In a third experiment, two of four alpha-toxin-negative mutants of CP4 protected birds from experimental NE after oral immunization. These two mutants were also attenuated for virulence. We conclude that it is possible to immunize chickens successfully against NE and that immunogen(s) other than alpha-toxin are important in protective immunity against oral infection. |
| doi_str_mv | 10.1016/j.vetmic.2005.10.015 |
| format | Article |
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Clostridium perfringens isolated from cases of NE in broiler chickens. Using a 5-day experimental oral infection of 2-week-old broiler chickens, four of the six strains were found to be virulent. Pulsed-field gel electrophoresis and PCR showed that virulence was not associated with a plasmid encoding the beta2 toxin gene,
cpb2, since this was present in virulent and one of the two avirulent strains.
In the second experiment, two virulent and one avirulent strains were tested for their ability to immunize (“infection-immunization”) chickens through the oral route. The procedure used experimental infection for 5 days followed by bacitracin treatment for 9 days, and then re-challenge 2 days later with a virulent strain, CP4. Infection-immunization with the virulent isolates protected chickens from subsequent virulent challenge, whereas the infection-immunization with the avirulent isolate did not. In a third experiment, two of four alpha-toxin-negative mutants of CP4 protected birds from experimental NE after oral immunization. These two mutants were also attenuated for virulence. We conclude that it is possible to immunize chickens successfully against NE and that immunogen(s) other than alpha-toxin are important in protective immunity against oral infection.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2005.10.015</identifier><identifier>PMID: 16289639</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Alpha-toxin mutants ; animal pathogenic bacteria ; Animals ; Anti-Bacterial Agents - therapeutic use ; antibiotics ; Applied microbiology ; bacitracin ; Bacitracin - therapeutic use ; bacterial antigens ; bacterial infections ; bacterial toxins ; Bacterial Vaccines ; Biological and medical sciences ; broiler chickens ; Chickens ; Clostridium Infections - prevention & control ; Clostridium Infections - veterinary ; Clostridium perfringens ; Clostridium perfringens - drug effects ; Clostridium perfringens - immunology ; Clostridium perfringens - pathogenicity ; disease control ; DNA Primers - chemistry ; Electrophoresis, Gel, Pulsed-Field - veterinary ; enteritis ; Enteritis - prevention & control ; Enteritis - veterinary ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Genotype ; immune response ; immunity ; live vaccines ; Microbiology ; Molecular Sequence Data ; Mutation - genetics ; Necrosis ; Necrotic enteritis ; poultry diseases ; Poultry Diseases - pathology ; Poultry Diseases - prevention & control ; Reverse Transcriptase Polymerase Chain Reaction - veterinary ; Sequence Analysis, DNA ; strain differences ; strains ; Type C Phospholipases - biosynthesis ; Type C Phospholipases - genetics ; Type C Phospholipases - immunology ; Vaccination ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vaccines, Attenuated ; Virulence</subject><ispartof>Veterinary microbiology, 2006-03, Vol.113 (1), p.25-34</ispartof><rights>2005 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-66ceb15514e2caba8865c98f673196540606dcb13bc1e6e30089628defd15d33</citedby><cites>FETCH-LOGICAL-c445t-66ceb15514e2caba8865c98f673196540606dcb13bc1e6e30089628defd15d33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378113505003214$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17537992$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16289639$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thompson, D.R.</creatorcontrib><creatorcontrib>Parreira, V.R.</creatorcontrib><creatorcontrib>Kulkarni, R.R.</creatorcontrib><creatorcontrib>Prescott, J.F.</creatorcontrib><title>Live attenuated vaccine-based control of necrotic enteritis of broiler chickens</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>A vaccine for necrotic enteritis (NE) of chickens would reduce the current need to prevent or treat the disease in broiler chickens with antimicrobial drugs. The objective of this study was to understand aspects of immunity to the disease. The first experiment examined the virulence of six strains of
Clostridium perfringens isolated from cases of NE in broiler chickens. Using a 5-day experimental oral infection of 2-week-old broiler chickens, four of the six strains were found to be virulent. Pulsed-field gel electrophoresis and PCR showed that virulence was not associated with a plasmid encoding the beta2 toxin gene,
cpb2, since this was present in virulent and one of the two avirulent strains.
In the second experiment, two virulent and one avirulent strains were tested for their ability to immunize (“infection-immunization”) chickens through the oral route. The procedure used experimental infection for 5 days followed by bacitracin treatment for 9 days, and then re-challenge 2 days later with a virulent strain, CP4. Infection-immunization with the virulent isolates protected chickens from subsequent virulent challenge, whereas the infection-immunization with the avirulent isolate did not. In a third experiment, two of four alpha-toxin-negative mutants of CP4 protected birds from experimental NE after oral immunization. These two mutants were also attenuated for virulence. We conclude that it is possible to immunize chickens successfully against NE and that immunogen(s) other than alpha-toxin are important in protective immunity against oral infection.</description><subject>Alpha-toxin mutants</subject><subject>animal pathogenic bacteria</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - therapeutic use</subject><subject>antibiotics</subject><subject>Applied microbiology</subject><subject>bacitracin</subject><subject>Bacitracin - therapeutic use</subject><subject>bacterial antigens</subject><subject>bacterial infections</subject><subject>bacterial toxins</subject><subject>Bacterial Vaccines</subject><subject>Biological and medical sciences</subject><subject>broiler chickens</subject><subject>Chickens</subject><subject>Clostridium Infections - prevention & control</subject><subject>Clostridium Infections - veterinary</subject><subject>Clostridium perfringens</subject><subject>Clostridium perfringens - drug effects</subject><subject>Clostridium perfringens - immunology</subject><subject>Clostridium perfringens - pathogenicity</subject><subject>disease control</subject><subject>DNA Primers - chemistry</subject><subject>Electrophoresis, Gel, Pulsed-Field - veterinary</subject><subject>enteritis</subject><subject>Enteritis - prevention & control</subject><subject>Enteritis - veterinary</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>immune response</subject><subject>immunity</subject><subject>live vaccines</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mutation - genetics</subject><subject>Necrosis</subject><subject>Necrotic enteritis</subject><subject>poultry diseases</subject><subject>Poultry Diseases - pathology</subject><subject>Poultry Diseases - prevention & control</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - veterinary</subject><subject>Sequence Analysis, DNA</subject><subject>strain differences</subject><subject>strains</subject><subject>Type C Phospholipases - biosynthesis</subject><subject>Type C Phospholipases - genetics</subject><subject>Type C Phospholipases - immunology</subject><subject>Vaccination</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Vaccines, Attenuated</subject><subject>Virulence</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFu1DAQhi1ERZfCGyDIpdyyeOLYiS9IqCpQaaUeKGfLmUzAS9Yutncl3h5HWak3OFn-9f2jmY-xN8C3wEF92G9PlA8Otw3nskRbDvIZ20DfibqRbfOcbbjo-hpAyEv2MqU957zVir9gl6CaXiuhN-x-505U2ZzJH22msTpZROepHmwqPww-xzBXYao8YQzZYUU-U3TZpSUdYnAzxQp_OvxFPr1iF5OdE70-v1fs4fPtw83Xenf_5e7m067GtpW5VgppACmhpQbtYPteSdT9pDoBWsmWK65GHEAMCKRIcF72bfqRphHkKMQVe7-OfYzh95FSNgeXkObZegrHZFSnpBKi-S9Y5GktOyhgu4LlypQiTeYxuoONfwxwswg3e7MKXzpySYvwUnt7nn8cDjQ-lc6GC3B9BmxCO0_RenTpieuk6LReFn23cpMNxv6Ihfn-reEgOMAC9YX4uBJUvJ4cRZPQkUcaXSTMZgzu37v-BbTGqWk</recordid><startdate>20060310</startdate><enddate>20060310</enddate><creator>Thompson, D.R.</creator><creator>Parreira, V.R.</creator><creator>Kulkarni, R.R.</creator><creator>Prescott, J.F.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060310</creationdate><title>Live attenuated vaccine-based control of necrotic enteritis of broiler chickens</title><author>Thompson, D.R. ; Parreira, V.R. ; Kulkarni, R.R. ; Prescott, J.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-66ceb15514e2caba8865c98f673196540606dcb13bc1e6e30089628defd15d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Alpha-toxin mutants</topic><topic>animal pathogenic bacteria</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - therapeutic use</topic><topic>antibiotics</topic><topic>Applied microbiology</topic><topic>bacitracin</topic><topic>Bacitracin - therapeutic use</topic><topic>bacterial antigens</topic><topic>bacterial infections</topic><topic>bacterial toxins</topic><topic>Bacterial Vaccines</topic><topic>Biological and medical sciences</topic><topic>broiler chickens</topic><topic>Chickens</topic><topic>Clostridium Infections - prevention & control</topic><topic>Clostridium Infections - veterinary</topic><topic>Clostridium perfringens</topic><topic>Clostridium perfringens - drug effects</topic><topic>Clostridium perfringens - immunology</topic><topic>Clostridium perfringens - pathogenicity</topic><topic>disease control</topic><topic>DNA Primers - chemistry</topic><topic>Electrophoresis, Gel, Pulsed-Field - veterinary</topic><topic>enteritis</topic><topic>Enteritis - prevention & control</topic><topic>Enteritis - veterinary</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>immune response</topic><topic>immunity</topic><topic>live vaccines</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mutation - genetics</topic><topic>Necrosis</topic><topic>Necrotic enteritis</topic><topic>poultry diseases</topic><topic>Poultry Diseases - pathology</topic><topic>Poultry Diseases - prevention & control</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - veterinary</topic><topic>Sequence Analysis, DNA</topic><topic>strain differences</topic><topic>strains</topic><topic>Type C Phospholipases - biosynthesis</topic><topic>Type C Phospholipases - genetics</topic><topic>Type C Phospholipases - immunology</topic><topic>Vaccination</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Vaccines, Attenuated</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thompson, D.R.</creatorcontrib><creatorcontrib>Parreira, V.R.</creatorcontrib><creatorcontrib>Kulkarni, R.R.</creatorcontrib><creatorcontrib>Prescott, J.F.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thompson, D.R.</au><au>Parreira, V.R.</au><au>Kulkarni, R.R.</au><au>Prescott, J.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Live attenuated vaccine-based control of necrotic enteritis of broiler chickens</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2006-03-10</date><risdate>2006</risdate><volume>113</volume><issue>1</issue><spage>25</spage><epage>34</epage><pages>25-34</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>A vaccine for necrotic enteritis (NE) of chickens would reduce the current need to prevent or treat the disease in broiler chickens with antimicrobial drugs. The objective of this study was to understand aspects of immunity to the disease. The first experiment examined the virulence of six strains of
Clostridium perfringens isolated from cases of NE in broiler chickens. Using a 5-day experimental oral infection of 2-week-old broiler chickens, four of the six strains were found to be virulent. Pulsed-field gel electrophoresis and PCR showed that virulence was not associated with a plasmid encoding the beta2 toxin gene,
cpb2, since this was present in virulent and one of the two avirulent strains.
In the second experiment, two virulent and one avirulent strains were tested for their ability to immunize (“infection-immunization”) chickens through the oral route. The procedure used experimental infection for 5 days followed by bacitracin treatment for 9 days, and then re-challenge 2 days later with a virulent strain, CP4. Infection-immunization with the virulent isolates protected chickens from subsequent virulent challenge, whereas the infection-immunization with the avirulent isolate did not. In a third experiment, two of four alpha-toxin-negative mutants of CP4 protected birds from experimental NE after oral immunization. These two mutants were also attenuated for virulence. We conclude that it is possible to immunize chickens successfully against NE and that immunogen(s) other than alpha-toxin are important in protective immunity against oral infection.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16289639</pmid><doi>10.1016/j.vetmic.2005.10.015</doi><tpages>10</tpages></addata></record> |
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| subjects | Alpha-toxin mutants animal pathogenic bacteria Animals Anti-Bacterial Agents - therapeutic use antibiotics Applied microbiology bacitracin Bacitracin - therapeutic use bacterial antigens bacterial infections bacterial toxins Bacterial Vaccines Biological and medical sciences broiler chickens Chickens Clostridium Infections - prevention & control Clostridium Infections - veterinary Clostridium perfringens Clostridium perfringens - drug effects Clostridium perfringens - immunology Clostridium perfringens - pathogenicity disease control DNA Primers - chemistry Electrophoresis, Gel, Pulsed-Field - veterinary enteritis Enteritis - prevention & control Enteritis - veterinary Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genotype immune response immunity live vaccines Microbiology Molecular Sequence Data Mutation - genetics Necrosis Necrotic enteritis poultry diseases Poultry Diseases - pathology Poultry Diseases - prevention & control Reverse Transcriptase Polymerase Chain Reaction - veterinary Sequence Analysis, DNA strain differences strains Type C Phospholipases - biosynthesis Type C Phospholipases - genetics Type C Phospholipases - immunology Vaccination Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, Attenuated Virulence |
| title | Live attenuated vaccine-based control of necrotic enteritis of broiler chickens |
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