Protein depletion from blood plasma using a volatile buffer
Removal of high abundance proteins is widely used in sample processing for proteomics studies of blood plasma. Immunoaffinity (IA) depletion is currently the most specific method for performing this step. Historically, IA depletion matrices have been designed to be used with inorganic buffers. Howev...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2006-02, Vol.832 (1), p.41-46 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
| container_volume | 832 |
| creator | Sitnikov, Dmitri Chan, Donovan Thibaudeau, Eric Pinard, Marc Hunter, Joanna M. |
| description | Removal of high abundance proteins is widely used in sample processing for proteomics studies of blood plasma. Immunoaffinity (IA) depletion is currently the most specific method for performing this step. Historically, IA depletion matrices have been designed to be used with inorganic buffers. However, the presence of salts in depleted samples presents a particular problem, and these must be removed in order to make samples compatible with post-depletion processing. Desalting (dialysis, ultrafiltration, size-exclusion, etc.) usually diminishes sample integrity due to labware associated losses. Moreover, these steps require additional labor, increasing the processing time and cost of analysis. In order to avoid these problems, we have developed an IA method using a volatile buffer that can be removed from depleted samples by lyophilization. This method allows the execution of reproducible and efficient depletion of blood plasma in a semi-automated manner. |
| doi_str_mv | 10.1016/j.jchromb.2005.12.013 |
| format | Article |
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Immunoaffinity (IA) depletion is currently the most specific method for performing this step. Historically, IA depletion matrices have been designed to be used with inorganic buffers. However, the presence of salts in depleted samples presents a particular problem, and these must be removed in order to make samples compatible with post-depletion processing. Desalting (dialysis, ultrafiltration, size-exclusion, etc.) usually diminishes sample integrity due to labware associated losses. Moreover, these steps require additional labor, increasing the processing time and cost of analysis. In order to avoid these problems, we have developed an IA method using a volatile buffer that can be removed from depleted samples by lyophilization. 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Drug treatments ; Plasma ; Plasma profiling ; Proteomics ; Reproducibility of Results ; Ultrafiltration ; Volatile buffer</subject><ispartof>Journal of chromatography. 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B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>Removal of high abundance proteins is widely used in sample processing for proteomics studies of blood plasma. Immunoaffinity (IA) depletion is currently the most specific method for performing this step. Historically, IA depletion matrices have been designed to be used with inorganic buffers. However, the presence of salts in depleted samples presents a particular problem, and these must be removed in order to make samples compatible with post-depletion processing. Desalting (dialysis, ultrafiltration, size-exclusion, etc.) usually diminishes sample integrity due to labware associated losses. Moreover, these steps require additional labor, increasing the processing time and cost of analysis. In order to avoid these problems, we have developed an IA method using a volatile buffer that can be removed from depleted samples by lyophilization. This method allows the execution of reproducible and efficient depletion of blood plasma in a semi-automated manner.</description><subject>Albumin</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Blood Proteins - isolation & purification</subject><subject>Buffers</subject><subject>Chromatography, Ion Exchange</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Freeze Drying</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Immunoaffinity depletion</subject><subject>Immunoglobulin G</subject><subject>Medical sciences</subject><subject>Multiple affinity removal system</subject><subject>Pharmacology. Drug treatments</subject><subject>Plasma</subject><subject>Plasma profiling</subject><subject>Proteomics</subject><subject>Reproducibility of Results</subject><subject>Ultrafiltration</subject><subject>Volatile buffer</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVpSdI0P6HFl-ZmV6MvW_RQQugXBNpDC70JrTxKtMjWVrID-ffVsoYce5qBed6Z4SHkLdAOKKgP-27vHnKadh2jVHbAOgr8BbmAoect79Wfl7WXPW0p4-ycvC5lTyn0tOdn5ByUAMFBXpCPP3NaMMzNiIeIS0hz4-vWZhdTGptDtGWyzVrCfN_Y5jFFu4SIzW71HvMb8srbWPBqq5fk95fPv26_tXc_vn6_vblrnWBsaQcJwGyvx1F4pQfgzI_UqkFprrwCCVyhp6yOUVGtBQNNvddCeq0dZY5fkuvT3kNOf1csi5lCcRijnTGtxaheSSkEVFCeQJdTKRm9OeQw2fxkgJqjNbM3mzVztGaAmWqt5t5tB9bdhONzatNUgfcbYIuz0Wc7u1CeuV4KkIOo3KcTh1XHY8Bsigs4OxxDRreYMYX_vPIPg0eL6Q</recordid><startdate>20060217</startdate><enddate>20060217</enddate><creator>Sitnikov, Dmitri</creator><creator>Chan, Donovan</creator><creator>Thibaudeau, Eric</creator><creator>Pinard, Marc</creator><creator>Hunter, Joanna M.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060217</creationdate><title>Protein depletion from blood plasma using a volatile buffer</title><author>Sitnikov, Dmitri ; Chan, Donovan ; Thibaudeau, Eric ; Pinard, Marc ; Hunter, Joanna M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-85112a79dd4f698132fd0a686936f615136ef02dd4e609942190ff945f99c02c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Albumin</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Blood Proteins - isolation & purification</topic><topic>Buffers</topic><topic>Chromatography, Ion Exchange</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Freeze Drying</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Immunoaffinity depletion</topic><topic>Immunoglobulin G</topic><topic>Medical sciences</topic><topic>Multiple affinity removal system</topic><topic>Pharmacology. 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| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2006-02, Vol.832 (1), p.41-46 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Albumin Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Blood Proteins - isolation & purification Buffers Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Freeze Drying Fundamental and applied biological sciences. Psychology General pharmacology Humans Immunoaffinity depletion Immunoglobulin G Medical sciences Multiple affinity removal system Pharmacology. Drug treatments Plasma Plasma profiling Proteomics Reproducibility of Results Ultrafiltration Volatile buffer |
| title | Protein depletion from blood plasma using a volatile buffer |
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