Nitro-fatty Acids Occur in Human Plasma in the Picomolar Range: a Targeted Nitro-lipidomics GC-MS/MS Study
First studies on the occurrence of nitrated fatty acids in plasma of healthy subjects revealed basal concentrations of 600 nM for free/nonesterified nitro-oleic acid (NO₂-OA) as measured by liquid chromatography tandem mass spectrometry (LC-MS/MS). We recently showed by a gas chromatography tandem m...
Gespeichert in:
| Veröffentlicht in: | Lipids 2009-09, Vol.44 (9), p.855-865 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 865 |
|---|---|
| container_issue | 9 |
| container_start_page | 855 |
| container_title | Lipids |
| container_volume | 44 |
| creator | Tsikas, Dimitrios Zoerner, Alexander A Mitschke, Anja Gutzki, Frank-Mathias |
| description | First studies on the occurrence of nitrated fatty acids in plasma of healthy subjects revealed basal concentrations of 600 nM for free/nonesterified nitro-oleic acid (NO₂-OA) as measured by liquid chromatography tandem mass spectrometry (LC-MS/MS). We recently showed by a gas chromatography tandem mass spectrometry (GC-MS/MS) method the physiological occurrence of two isomers, i.e., 9-NO₂-OA and 10-NO₂-OA, at mean basal plasma concentrations of 880 and 940 pM, respectively. In consideration of this large discrepancy we modified our originally reported method by replacing solid-phase extraction (SPE) by solvent extraction with ethyl acetate and by omitting the high-performance liquid chromatography (HPLC) step for a more direct detection and with the potential for lipidomics studies. Intra-assay imprecision and accuracy of the modified method in human plasma were 1-34% and 91-221%, respectively, for added NO₂-OA concentrations in the range 0-3,000 pM. This method provided basal plasma concentrations of 306 ± 44 pM for 9-NO₂-OA and 316 ± 33 pM for 10-NO₂-OA in 15 healthy subjects. Nitro-arachidonic acid and nitro-linolenic acid were not detectable in the plasma samples. In summary, our studies show 9-NO₂-OA and 10-NO₂-OA as endogenous nitrated fatty acids in human plasma in the pM range; HPLC is recommendable as a sample clean-up step for reliable quantification of nitro-oleic acids by GC-MS/MS. |
| doi_str_mv | 10.1007/s11745-009-3332-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67653446</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67653446</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5075-876a6648a3582affc251f5f52fa8fb07f068072906413eb0e1fcb3d4b74647df3</originalsourceid><addsrcrecordid>eNqFkk1rFDEAhoModq3-AC8aPPQWm-9kvJW1toWtXdz2HDKZZM0yH2syg-y_N8ssFDzoKQSe5-UlbwB4T_BngrG6zIQoLhDGFWKMUcRfgAURQqOKYfUSLDCmHHGKyRl4k_OuXAmvxGtwRiqFiRRqAXbf45gGFOw4HuCVi02GD85NCcYe3k6d7eG6tbmzx_v408N1dEM3tDbBH7bf-i_Qwkebtn70DZyj2riPzdBFl-HNEt1vLu83cDNOzeEteBVsm_2703kOnr5dPy5v0erh5m55tUJOYCWQVtJKybVlQlMbgqOCBBEEDVaHGquApcaKVlhywnyNPQmuZg2vFZdcNYGdg4s5d5-GX5PPo-lidr5tbe-HKRuppGCcywJ--gvcDVPqSzdDS5iQkhwhMkMuDTknH8w-xc6mgyHYHFcw8wqmrGCOKxhenA-n4KnufPNsnJ69AGoGfsfWH_6faFZ3669YC1FMOpu5SGWA9Nz5X30-zlKwg7HbFLN52pRvwUobjbWi7A_ceKln</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>274656616</pqid></control><display><type>article</type><title>Nitro-fatty Acids Occur in Human Plasma in the Picomolar Range: a Targeted Nitro-lipidomics GC-MS/MS Study</title><source>MEDLINE</source><source>Wiley Online Library</source><source>Springer Journals</source><creator>Tsikas, Dimitrios ; Zoerner, Alexander A ; Mitschke, Anja ; Gutzki, Frank-Mathias</creator><creatorcontrib>Tsikas, Dimitrios ; Zoerner, Alexander A ; Mitschke, Anja ; Gutzki, Frank-Mathias</creatorcontrib><description>First studies on the occurrence of nitrated fatty acids in plasma of healthy subjects revealed basal concentrations of 600 nM for free/nonesterified nitro-oleic acid (NO₂-OA) as measured by liquid chromatography tandem mass spectrometry (LC-MS/MS). We recently showed by a gas chromatography tandem mass spectrometry (GC-MS/MS) method the physiological occurrence of two isomers, i.e., 9-NO₂-OA and 10-NO₂-OA, at mean basal plasma concentrations of 880 and 940 pM, respectively. In consideration of this large discrepancy we modified our originally reported method by replacing solid-phase extraction (SPE) by solvent extraction with ethyl acetate and by omitting the high-performance liquid chromatography (HPLC) step for a more direct detection and with the potential for lipidomics studies. Intra-assay imprecision and accuracy of the modified method in human plasma were 1-34% and 91-221%, respectively, for added NO₂-OA concentrations in the range 0-3,000 pM. This method provided basal plasma concentrations of 306 ± 44 pM for 9-NO₂-OA and 316 ± 33 pM for 10-NO₂-OA in 15 healthy subjects. Nitro-arachidonic acid and nitro-linolenic acid were not detectable in the plasma samples. In summary, our studies show 9-NO₂-OA and 10-NO₂-OA as endogenous nitrated fatty acids in human plasma in the pM range; HPLC is recommendable as a sample clean-up step for reliable quantification of nitro-oleic acids by GC-MS/MS.</description><identifier>ISSN: 0024-4201</identifier><identifier>EISSN: 1558-9307</identifier><identifier>DOI: 10.1007/s11745-009-3332-4</identifier><identifier>PMID: 19701657</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Biomedical and Life Sciences ; blood chemistry ; blood plasma ; chemical analysis ; Fatty acids ; Fatty Acids - blood ; Gas chromatography ; Gas Chromatography-Mass Spectrometry - methods ; GC–MS/MS ; HPLC ; human health ; human physiology ; Humans ; Interferences ; LC–MS/MS ; Life Sciences ; Lipidology ; Lipidomics ; Liquid chromatography ; Mass spectrometry ; Medical Biochemistry ; Medicinal Chemistry ; Microbial Genetics and Genomics ; Neurochemistry ; Nitration ; nitrification ; Nitro Compounds - blood ; Nutrition ; oleic acid ; Original Article ; Quantification ; Sensitivity and Specificity ; Solvent extraction ; spectral analysis ; Stable isotopes ; Tandem Mass Spectrometry - methods ; Validation</subject><ispartof>Lipids, 2009-09, Vol.44 (9), p.855-865</ispartof><rights>AOCS 2009</rights><rights>2009 American Oil Chemists' Society (AOCS)</rights><rights>Copyright AOCS Press Sep 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5075-876a6648a3582affc251f5f52fa8fb07f068072906413eb0e1fcb3d4b74647df3</citedby><cites>FETCH-LOGICAL-c5075-876a6648a3582affc251f5f52fa8fb07f068072906413eb0e1fcb3d4b74647df3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11745-009-3332-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11745-009-3332-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,41467,42536,45553,45554,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19701657$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsikas, Dimitrios</creatorcontrib><creatorcontrib>Zoerner, Alexander A</creatorcontrib><creatorcontrib>Mitschke, Anja</creatorcontrib><creatorcontrib>Gutzki, Frank-Mathias</creatorcontrib><title>Nitro-fatty Acids Occur in Human Plasma in the Picomolar Range: a Targeted Nitro-lipidomics GC-MS/MS Study</title><title>Lipids</title><addtitle>Lipids</addtitle><addtitle>Lipids</addtitle><description>First studies on the occurrence of nitrated fatty acids in plasma of healthy subjects revealed basal concentrations of 600 nM for free/nonesterified nitro-oleic acid (NO₂-OA) as measured by liquid chromatography tandem mass spectrometry (LC-MS/MS). We recently showed by a gas chromatography tandem mass spectrometry (GC-MS/MS) method the physiological occurrence of two isomers, i.e., 9-NO₂-OA and 10-NO₂-OA, at mean basal plasma concentrations of 880 and 940 pM, respectively. In consideration of this large discrepancy we modified our originally reported method by replacing solid-phase extraction (SPE) by solvent extraction with ethyl acetate and by omitting the high-performance liquid chromatography (HPLC) step for a more direct detection and with the potential for lipidomics studies. Intra-assay imprecision and accuracy of the modified method in human plasma were 1-34% and 91-221%, respectively, for added NO₂-OA concentrations in the range 0-3,000 pM. This method provided basal plasma concentrations of 306 ± 44 pM for 9-NO₂-OA and 316 ± 33 pM for 10-NO₂-OA in 15 healthy subjects. Nitro-arachidonic acid and nitro-linolenic acid were not detectable in the plasma samples. In summary, our studies show 9-NO₂-OA and 10-NO₂-OA as endogenous nitrated fatty acids in human plasma in the pM range; HPLC is recommendable as a sample clean-up step for reliable quantification of nitro-oleic acids by GC-MS/MS.</description><subject>Biomedical and Life Sciences</subject><subject>blood chemistry</subject><subject>blood plasma</subject><subject>chemical analysis</subject><subject>Fatty acids</subject><subject>Fatty Acids - blood</subject><subject>Gas chromatography</subject><subject>Gas Chromatography-Mass Spectrometry - methods</subject><subject>GC–MS/MS</subject><subject>HPLC</subject><subject>human health</subject><subject>human physiology</subject><subject>Humans</subject><subject>Interferences</subject><subject>LC–MS/MS</subject><subject>Life Sciences</subject><subject>Lipidology</subject><subject>Lipidomics</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Medical Biochemistry</subject><subject>Medicinal Chemistry</subject><subject>Microbial Genetics and Genomics</subject><subject>Neurochemistry</subject><subject>Nitration</subject><subject>nitrification</subject><subject>Nitro Compounds - blood</subject><subject>Nutrition</subject><subject>oleic acid</subject><subject>Original Article</subject><subject>Quantification</subject><subject>Sensitivity and Specificity</subject><subject>Solvent extraction</subject><subject>spectral analysis</subject><subject>Stable isotopes</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Validation</subject><issn>0024-4201</issn><issn>1558-9307</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkk1rFDEAhoModq3-AC8aPPQWm-9kvJW1toWtXdz2HDKZZM0yH2syg-y_N8ssFDzoKQSe5-UlbwB4T_BngrG6zIQoLhDGFWKMUcRfgAURQqOKYfUSLDCmHHGKyRl4k_OuXAmvxGtwRiqFiRRqAXbf45gGFOw4HuCVi02GD85NCcYe3k6d7eG6tbmzx_v408N1dEM3tDbBH7bf-i_Qwkebtn70DZyj2riPzdBFl-HNEt1vLu83cDNOzeEteBVsm_2703kOnr5dPy5v0erh5m55tUJOYCWQVtJKybVlQlMbgqOCBBEEDVaHGquApcaKVlhywnyNPQmuZg2vFZdcNYGdg4s5d5-GX5PPo-lidr5tbe-HKRuppGCcywJ--gvcDVPqSzdDS5iQkhwhMkMuDTknH8w-xc6mgyHYHFcw8wqmrGCOKxhenA-n4KnufPNsnJ69AGoGfsfWH_6faFZ3669YC1FMOpu5SGWA9Nz5X30-zlKwg7HbFLN52pRvwUobjbWi7A_ceKln</recordid><startdate>200909</startdate><enddate>200909</enddate><creator>Tsikas, Dimitrios</creator><creator>Zoerner, Alexander A</creator><creator>Mitschke, Anja</creator><creator>Gutzki, Frank-Mathias</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer‐Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>200909</creationdate><title>Nitro-fatty Acids Occur in Human Plasma in the Picomolar Range: a Targeted Nitro-lipidomics GC-MS/MS Study</title><author>Tsikas, Dimitrios ; Zoerner, Alexander A ; Mitschke, Anja ; Gutzki, Frank-Mathias</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5075-876a6648a3582affc251f5f52fa8fb07f068072906413eb0e1fcb3d4b74647df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Biomedical and Life Sciences</topic><topic>blood chemistry</topic><topic>blood plasma</topic><topic>chemical analysis</topic><topic>Fatty acids</topic><topic>Fatty Acids - blood</topic><topic>Gas chromatography</topic><topic>Gas Chromatography-Mass Spectrometry - methods</topic><topic>GC–MS/MS</topic><topic>HPLC</topic><topic>human health</topic><topic>human physiology</topic><topic>Humans</topic><topic>Interferences</topic><topic>LC–MS/MS</topic><topic>Life Sciences</topic><topic>Lipidology</topic><topic>Lipidomics</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Medical Biochemistry</topic><topic>Medicinal Chemistry</topic><topic>Microbial Genetics and Genomics</topic><topic>Neurochemistry</topic><topic>Nitration</topic><topic>nitrification</topic><topic>Nitro Compounds - blood</topic><topic>Nutrition</topic><topic>oleic acid</topic><topic>Original Article</topic><topic>Quantification</topic><topic>Sensitivity and Specificity</topic><topic>Solvent extraction</topic><topic>spectral analysis</topic><topic>Stable isotopes</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Validation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsikas, Dimitrios</creatorcontrib><creatorcontrib>Zoerner, Alexander A</creatorcontrib><creatorcontrib>Mitschke, Anja</creatorcontrib><creatorcontrib>Gutzki, Frank-Mathias</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Databases</collection><collection>ProQuest Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Lipids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsikas, Dimitrios</au><au>Zoerner, Alexander A</au><au>Mitschke, Anja</au><au>Gutzki, Frank-Mathias</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitro-fatty Acids Occur in Human Plasma in the Picomolar Range: a Targeted Nitro-lipidomics GC-MS/MS Study</atitle><jtitle>Lipids</jtitle><stitle>Lipids</stitle><addtitle>Lipids</addtitle><date>2009-09</date><risdate>2009</risdate><volume>44</volume><issue>9</issue><spage>855</spage><epage>865</epage><pages>855-865</pages><issn>0024-4201</issn><eissn>1558-9307</eissn><abstract>First studies on the occurrence of nitrated fatty acids in plasma of healthy subjects revealed basal concentrations of 600 nM for free/nonesterified nitro-oleic acid (NO₂-OA) as measured by liquid chromatography tandem mass spectrometry (LC-MS/MS). We recently showed by a gas chromatography tandem mass spectrometry (GC-MS/MS) method the physiological occurrence of two isomers, i.e., 9-NO₂-OA and 10-NO₂-OA, at mean basal plasma concentrations of 880 and 940 pM, respectively. In consideration of this large discrepancy we modified our originally reported method by replacing solid-phase extraction (SPE) by solvent extraction with ethyl acetate and by omitting the high-performance liquid chromatography (HPLC) step for a more direct detection and with the potential for lipidomics studies. Intra-assay imprecision and accuracy of the modified method in human plasma were 1-34% and 91-221%, respectively, for added NO₂-OA concentrations in the range 0-3,000 pM. This method provided basal plasma concentrations of 306 ± 44 pM for 9-NO₂-OA and 316 ± 33 pM for 10-NO₂-OA in 15 healthy subjects. Nitro-arachidonic acid and nitro-linolenic acid were not detectable in the plasma samples. In summary, our studies show 9-NO₂-OA and 10-NO₂-OA as endogenous nitrated fatty acids in human plasma in the pM range; HPLC is recommendable as a sample clean-up step for reliable quantification of nitro-oleic acids by GC-MS/MS.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>19701657</pmid><doi>10.1007/s11745-009-3332-4</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0024-4201 |
| ispartof | Lipids, 2009-09, Vol.44 (9), p.855-865 |
| issn | 0024-4201 1558-9307 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67653446 |
| source | MEDLINE; Wiley Online Library; Springer Journals |
| subjects | Biomedical and Life Sciences blood chemistry blood plasma chemical analysis Fatty acids Fatty Acids - blood Gas chromatography Gas Chromatography-Mass Spectrometry - methods GC–MS/MS HPLC human health human physiology Humans Interferences LC–MS/MS Life Sciences Lipidology Lipidomics Liquid chromatography Mass spectrometry Medical Biochemistry Medicinal Chemistry Microbial Genetics and Genomics Neurochemistry Nitration nitrification Nitro Compounds - blood Nutrition oleic acid Original Article Quantification Sensitivity and Specificity Solvent extraction spectral analysis Stable isotopes Tandem Mass Spectrometry - methods Validation |
| title | Nitro-fatty Acids Occur in Human Plasma in the Picomolar Range: a Targeted Nitro-lipidomics GC-MS/MS Study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T18%3A11%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nitro-fatty%20Acids%20Occur%20in%20Human%20Plasma%20in%20the%20Picomolar%20Range:%20a%20Targeted%20Nitro-lipidomics%20GC-MS/MS%20Study&rft.jtitle=Lipids&rft.au=Tsikas,%20Dimitrios&rft.date=2009-09&rft.volume=44&rft.issue=9&rft.spage=855&rft.epage=865&rft.pages=855-865&rft.issn=0024-4201&rft.eissn=1558-9307&rft_id=info:doi/10.1007/s11745-009-3332-4&rft_dat=%3Cproquest_cross%3E67653446%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=274656616&rft_id=info:pmid/19701657&rfr_iscdi=true |