The modulation of gene expression in osteoblasts by thrombin coated on biphasic calcium phosphate ceramic
For many years, fibrin sealants were associated with bone substitutes to promote bone healing. However, the osteoblastic response to fibrin sealant components remains poorly documented. In this study, MC3T3-E1 osteoblastic cells were cultured on biphasic calcium phosphate ceramic (MBCP ®) coated wit...
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| Veröffentlicht in: | Biomaterials 2006-05, Vol.27 (15), p.2934-2943 |
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| creator | Bluteau, Gilles Pilet, Paul Bourges, Xavier Bilban, Melitta Spaethe, Reiner Daculsi, Guy Guicheux, Jérôme |
| description | For many years, fibrin sealants were associated with bone substitutes to promote bone healing. However, the osteoblastic response to fibrin sealant components remains poorly documented. In this study, MC3T3-E1 osteoblastic cells were cultured on biphasic calcium phosphate ceramic (MBCP
®) coated with Tissucol
® components (thrombin and fibrinogen). Analysis of osteoblastic differentiation markers by RT-PCR revealed that MBCP
® coated with Tissucol
® stimulated mRNA levels for osteocalcin and alkaline phosphatase (ALP). Of all the components of Tissucol
®, thrombin has been reported to affect osteoblastic behavior. Our results demonstrated that low thrombin concentrations (0.5–5
U/ml) stimulated mRNA levels for ALP, whereas high thrombin concentrations (50–100
U/ml) decreased mRNA levels for ALP and PTH/PTHrP receptor and also increased mRNA level for the osteoclastogenesis inhibitor OPG. As thrombin stimulated angiogenesis, we then wondered whether thrombin could influence the expression of angiogenic factors. Low thrombin concentrations were shown to up-regulate mRNA levels for VEGF-B and VEGF-R1, suggesting an autocrine/paracrine role for VEGF-B. Higher thrombin concentrations also up-regulated mRNA for VEGF-A and neuropilin-1. In conclusion, the association of MBCP
® with thrombin and fibrinogen appears to be a convenient scaffold for bone cell differentiation. Thrombin could also acts at the cellular level by increasing the angiogenic potential of osteoblasts as well as their responsiveness to thrombin and VEGF. |
| doi_str_mv | 10.1016/j.biomaterials.2006.01.004 |
| format | Article |
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®) coated with Tissucol
® components (thrombin and fibrinogen). Analysis of osteoblastic differentiation markers by RT-PCR revealed that MBCP
® coated with Tissucol
® stimulated mRNA levels for osteocalcin and alkaline phosphatase (ALP). Of all the components of Tissucol
®, thrombin has been reported to affect osteoblastic behavior. Our results demonstrated that low thrombin concentrations (0.5–5
U/ml) stimulated mRNA levels for ALP, whereas high thrombin concentrations (50–100
U/ml) decreased mRNA levels for ALP and PTH/PTHrP receptor and also increased mRNA level for the osteoclastogenesis inhibitor OPG. As thrombin stimulated angiogenesis, we then wondered whether thrombin could influence the expression of angiogenic factors. Low thrombin concentrations were shown to up-regulate mRNA levels for VEGF-B and VEGF-R1, suggesting an autocrine/paracrine role for VEGF-B. Higher thrombin concentrations also up-regulated mRNA for VEGF-A and neuropilin-1. In conclusion, the association of MBCP
® with thrombin and fibrinogen appears to be a convenient scaffold for bone cell differentiation. Thrombin could also acts at the cellular level by increasing the angiogenic potential of osteoblasts as well as their responsiveness to thrombin and VEGF.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2006.01.004</identifier><identifier>PMID: 16436294</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Animals ; Biphasic calcium phosphate ; Bone Substitutes - administration & dosage ; Bone Substitutes - pharmacology ; Calcium Phosphates - chemistry ; Cell Differentiation - drug effects ; Cell Proliferation - drug effects ; Cell Size - drug effects ; Ceramics - chemistry ; Coated Materials, Biocompatible - administration & dosage ; Differentiation ; Fibrin glue ; Fibrin Tissue Adhesive - administration & dosage ; Fibrin Tissue Adhesive - chemistry ; Gene Expression Regulation - drug effects ; Gene Expression Regulation - physiology ; Mice ; NIH 3T3 Cells ; Osteoblast ; Osteoblasts - cytology ; Osteoblasts - drug effects ; Osteoblasts - physiology ; Receptors, Thrombin - metabolism ; Thrombin ; Thrombin - administration & dosage ; Thrombin - chemistry ; Vascular Endothelial Growth Factor A - metabolism ; VEGF</subject><ispartof>Biomaterials, 2006-05, Vol.27 (15), p.2934-2943</ispartof><rights>2006 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c506t-5f6cdd7301649d4d422e2189333e31d6b55c04e99c34db267d03c0e3000211273</citedby><cites>FETCH-LOGICAL-c506t-5f6cdd7301649d4d422e2189333e31d6b55c04e99c34db267d03c0e3000211273</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biomaterials.2006.01.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16436294$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bluteau, Gilles</creatorcontrib><creatorcontrib>Pilet, Paul</creatorcontrib><creatorcontrib>Bourges, Xavier</creatorcontrib><creatorcontrib>Bilban, Melitta</creatorcontrib><creatorcontrib>Spaethe, Reiner</creatorcontrib><creatorcontrib>Daculsi, Guy</creatorcontrib><creatorcontrib>Guicheux, Jérôme</creatorcontrib><title>The modulation of gene expression in osteoblasts by thrombin coated on biphasic calcium phosphate ceramic</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>For many years, fibrin sealants were associated with bone substitutes to promote bone healing. However, the osteoblastic response to fibrin sealant components remains poorly documented. In this study, MC3T3-E1 osteoblastic cells were cultured on biphasic calcium phosphate ceramic (MBCP
®) coated with Tissucol
® components (thrombin and fibrinogen). Analysis of osteoblastic differentiation markers by RT-PCR revealed that MBCP
® coated with Tissucol
® stimulated mRNA levels for osteocalcin and alkaline phosphatase (ALP). Of all the components of Tissucol
®, thrombin has been reported to affect osteoblastic behavior. Our results demonstrated that low thrombin concentrations (0.5–5
U/ml) stimulated mRNA levels for ALP, whereas high thrombin concentrations (50–100
U/ml) decreased mRNA levels for ALP and PTH/PTHrP receptor and also increased mRNA level for the osteoclastogenesis inhibitor OPG. As thrombin stimulated angiogenesis, we then wondered whether thrombin could influence the expression of angiogenic factors. Low thrombin concentrations were shown to up-regulate mRNA levels for VEGF-B and VEGF-R1, suggesting an autocrine/paracrine role for VEGF-B. Higher thrombin concentrations also up-regulated mRNA for VEGF-A and neuropilin-1. In conclusion, the association of MBCP
® with thrombin and fibrinogen appears to be a convenient scaffold for bone cell differentiation. Thrombin could also acts at the cellular level by increasing the angiogenic potential of osteoblasts as well as their responsiveness to thrombin and VEGF.</description><subject>Animals</subject><subject>Biphasic calcium phosphate</subject><subject>Bone Substitutes - administration & dosage</subject><subject>Bone Substitutes - pharmacology</subject><subject>Calcium Phosphates - chemistry</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Size - drug effects</subject><subject>Ceramics - chemistry</subject><subject>Coated Materials, Biocompatible - administration & dosage</subject><subject>Differentiation</subject><subject>Fibrin glue</subject><subject>Fibrin Tissue Adhesive - administration & dosage</subject><subject>Fibrin Tissue Adhesive - chemistry</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Gene Expression Regulation - physiology</subject><subject>Mice</subject><subject>NIH 3T3 Cells</subject><subject>Osteoblast</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - drug effects</subject><subject>Osteoblasts - physiology</subject><subject>Receptors, Thrombin - metabolism</subject><subject>Thrombin</subject><subject>Thrombin - administration & dosage</subject><subject>Thrombin - chemistry</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>VEGF</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EotvCX0AWh94Sxh9x1tyqAi1SJS7lbDn2LOtVsg62U7X_Hi-7Er2V02hePfP5EvKRQcuAqU-7dghxsgVTsGNuOYBqgbUA8hVZsXW_bjoN3WuyAiZ5oxXjZ-Q85x3UHCR_S86YkkJxLVck3G-RTtEvoy0h7mnc0F-4R4qPc8KcD1Koai4Yh9HmkunwRMs2xWmouot1C08rNIR5a3Nw1NnRhWWi8zbmKhWkDpOdgntH3mzquvj-FC_Iz29f769vm7sfN9-vr-4a14EqTbdRzvte1EOl9tJLzpGztRZCoGBeDV3nQKLWTkg_cNV7EA5QAABnjPfiglwe-84p_l4wFzOF7HAc7R7jko3qVSeY4i-CXHPouYYXQdZDrwTICn4-gi7FnBNuzJzCZNOTYWAO1pmdeW6dOVhngBn4W_zhNGUZJvT_Sk9eVeDLEcD6vYeAyWQXcO_Qh4SuGB_D_8z5A2-RsXU</recordid><startdate>20060501</startdate><enddate>20060501</enddate><creator>Bluteau, Gilles</creator><creator>Pilet, Paul</creator><creator>Bourges, Xavier</creator><creator>Bilban, Melitta</creator><creator>Spaethe, Reiner</creator><creator>Daculsi, Guy</creator><creator>Guicheux, Jérôme</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7QQ</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20060501</creationdate><title>The modulation of gene expression in osteoblasts by thrombin coated on biphasic calcium phosphate ceramic</title><author>Bluteau, Gilles ; Pilet, Paul ; Bourges, Xavier ; Bilban, Melitta ; Spaethe, Reiner ; Daculsi, Guy ; Guicheux, Jérôme</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c506t-5f6cdd7301649d4d422e2189333e31d6b55c04e99c34db267d03c0e3000211273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Biphasic calcium phosphate</topic><topic>Bone Substitutes - administration & dosage</topic><topic>Bone Substitutes - pharmacology</topic><topic>Calcium Phosphates - chemistry</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Size - drug effects</topic><topic>Ceramics - chemistry</topic><topic>Coated Materials, Biocompatible - administration & dosage</topic><topic>Differentiation</topic><topic>Fibrin glue</topic><topic>Fibrin Tissue Adhesive - administration & dosage</topic><topic>Fibrin Tissue Adhesive - chemistry</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Gene Expression Regulation - physiology</topic><topic>Mice</topic><topic>NIH 3T3 Cells</topic><topic>Osteoblast</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - drug effects</topic><topic>Osteoblasts - physiology</topic><topic>Receptors, Thrombin - metabolism</topic><topic>Thrombin</topic><topic>Thrombin - administration & dosage</topic><topic>Thrombin - chemistry</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>VEGF</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bluteau, Gilles</creatorcontrib><creatorcontrib>Pilet, Paul</creatorcontrib><creatorcontrib>Bourges, Xavier</creatorcontrib><creatorcontrib>Bilban, Melitta</creatorcontrib><creatorcontrib>Spaethe, Reiner</creatorcontrib><creatorcontrib>Daculsi, Guy</creatorcontrib><creatorcontrib>Guicheux, Jérôme</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bluteau, Gilles</au><au>Pilet, Paul</au><au>Bourges, Xavier</au><au>Bilban, Melitta</au><au>Spaethe, Reiner</au><au>Daculsi, Guy</au><au>Guicheux, Jérôme</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The modulation of gene expression in osteoblasts by thrombin coated on biphasic calcium phosphate ceramic</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2006-05-01</date><risdate>2006</risdate><volume>27</volume><issue>15</issue><spage>2934</spage><epage>2943</epage><pages>2934-2943</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>For many years, fibrin sealants were associated with bone substitutes to promote bone healing. However, the osteoblastic response to fibrin sealant components remains poorly documented. In this study, MC3T3-E1 osteoblastic cells were cultured on biphasic calcium phosphate ceramic (MBCP
®) coated with Tissucol
® components (thrombin and fibrinogen). Analysis of osteoblastic differentiation markers by RT-PCR revealed that MBCP
® coated with Tissucol
® stimulated mRNA levels for osteocalcin and alkaline phosphatase (ALP). Of all the components of Tissucol
®, thrombin has been reported to affect osteoblastic behavior. Our results demonstrated that low thrombin concentrations (0.5–5
U/ml) stimulated mRNA levels for ALP, whereas high thrombin concentrations (50–100
U/ml) decreased mRNA levels for ALP and PTH/PTHrP receptor and also increased mRNA level for the osteoclastogenesis inhibitor OPG. As thrombin stimulated angiogenesis, we then wondered whether thrombin could influence the expression of angiogenic factors. Low thrombin concentrations were shown to up-regulate mRNA levels for VEGF-B and VEGF-R1, suggesting an autocrine/paracrine role for VEGF-B. Higher thrombin concentrations also up-regulated mRNA for VEGF-A and neuropilin-1. In conclusion, the association of MBCP
® with thrombin and fibrinogen appears to be a convenient scaffold for bone cell differentiation. Thrombin could also acts at the cellular level by increasing the angiogenic potential of osteoblasts as well as their responsiveness to thrombin and VEGF.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>16436294</pmid><doi>10.1016/j.biomaterials.2006.01.004</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Biphasic calcium phosphate Bone Substitutes - administration & dosage Bone Substitutes - pharmacology Calcium Phosphates - chemistry Cell Differentiation - drug effects Cell Proliferation - drug effects Cell Size - drug effects Ceramics - chemistry Coated Materials, Biocompatible - administration & dosage Differentiation Fibrin glue Fibrin Tissue Adhesive - administration & dosage Fibrin Tissue Adhesive - chemistry Gene Expression Regulation - drug effects Gene Expression Regulation - physiology Mice NIH 3T3 Cells Osteoblast Osteoblasts - cytology Osteoblasts - drug effects Osteoblasts - physiology Receptors, Thrombin - metabolism Thrombin Thrombin - administration & dosage Thrombin - chemistry Vascular Endothelial Growth Factor A - metabolism VEGF |
| title | The modulation of gene expression in osteoblasts by thrombin coated on biphasic calcium phosphate ceramic |
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