TC10 and Insulin‐Stimulated Glucose Transport
Insulin stimulates glucose uptake in insulin‐responsive tissues by means of the translocation of the glucose transporter GLUT4 from intracellular sites to the plasma membrane. Two pathways are required, one involving activation of a phosphatidylinositol 3‐kinase (PI 3‐kinase) and downstream protein...
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| Veröffentlicht in: | Methods in Enzymology 2006, Vol.406, p.701-714 |
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| creator | Chiang, Shian‐Huey Chang, Louise Saltiel, Alan R. |
| description | Insulin stimulates glucose uptake in insulin‐responsive tissues by means of the translocation of the glucose transporter GLUT4 from intracellular sites to the plasma membrane. Two pathways are required, one involving activation of a phosphatidylinositol 3‐kinase (PI 3‐kinase) and downstream protein kinases, and one involving activation of the Rho‐family GTPase TC10. TC10 activation by insulin is catalyzed by the exchange factor C3G, which is translocated to lipid rafts along with its binding partner CrkII as a consequence of Cbl tyrosine phosphorylation by the insulin receptor. This activation of TC10 is dependent on localization of TC10 in the lipid raft subdomains of the plasma membrane. We describe experimental approaches using the insulin‐responsive cell line 3T3‐L1 adipocytes to study the role of TC10 in insulin‐stimulated glucose transport. |
| doi_str_mv | 10.1016/S0076-6879(06)06055-1 |
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Two pathways are required, one involving activation of a phosphatidylinositol 3‐kinase (PI 3‐kinase) and downstream protein kinases, and one involving activation of the Rho‐family GTPase TC10. TC10 activation by insulin is catalyzed by the exchange factor C3G, which is translocated to lipid rafts along with its binding partner CrkII as a consequence of Cbl tyrosine phosphorylation by the insulin receptor. This activation of TC10 is dependent on localization of TC10 in the lipid raft subdomains of the plasma membrane. We describe experimental approaches using the insulin‐responsive cell line 3T3‐L1 adipocytes to study the role of TC10 in insulin‐stimulated glucose transport.</description><subject>3T3-L1 Cells</subject><subject>Adipocytes - drug effects</subject><subject>Adipocytes - metabolism</subject><subject>Androstadienes - pharmacology</subject><subject>Animals</subject><subject>Cell Differentiation</subject><subject>Centrifugation, Density Gradient</subject><subject>Electroporation</subject><subject>Fluorescent Antibody Technique</subject><subject>Glucose Transporter Type 4 - metabolism</subject><subject>Glutathione Transferase - genetics</subject><subject>Insulin - pharmacology</subject><subject>Magnesium - pharmacology</subject><subject>Mice</subject><subject>Molecular biology</subject><subject>Phosphatidylinositol 3-Kinases - antagonists & inhibitors</subject><subject>Proteins</subject><subject>Recombinant Fusion Proteins</subject><subject>rho GTP-Binding Proteins - genetics</subject><subject>rho GTP-Binding Proteins - physiology</subject><issn>0076-6879</issn><issn>1557-7988</issn><isbn>9780121828110</isbn><isbn>0121828115</isbn><isbn>0080479391</isbn><isbn>9780080479392</isbn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU1uFDEQhc1_hjBHIJpVRBZNqtx22V4hNIIkUqQsMqwtj10tGnq6B7s7EjuOwBlzEjo_sKA2tXnfU716QrxFeI-AdHoNYKgia9w7oBMg0LrCJ-I1gAVlXO3wqVig1qYyztpnYumMBZRopUWE52Lxj38pFhYsobLaHohlKd9gnnr2AXglDpCUkeTcQpxu1gir0KfVRV-mru1vf_2-Htvd1IWR0-qsm-JQeLXJoS_7IY9vxIsmdIWXj_tQfPn8abM-ry6vzi7WHy-rqLQeK6dqR0kqKVNqdKSwtVY1aI1MpELS3MTA0lIwUkUdUWqnnEQKjWWula0PxfGD7z4PPyYuo9-1JXLXhZ6HqXgypOWcZhYePQqn7Y6T3-d2F_JP_zfiLJD_OfF2GL5H7sccuvg17EfOxcv55Np4Qm-UnqEPDxDPGW9azr7ElvvIqc0cR5-G1iP4u9b8fWv-7useyN-35rH-AyV5gGE</recordid><startdate>2006</startdate><enddate>2006</enddate><creator>Chiang, Shian‐Huey</creator><creator>Chang, Louise</creator><creator>Saltiel, Alan R.</creator><general>Elsevier Science & Technology</general><scope>FFUUA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>2006</creationdate><title>TC10 and Insulin‐Stimulated Glucose Transport</title><author>Chiang, Shian‐Huey ; Chang, Louise ; Saltiel, Alan R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-94396d2422ddf5c6ab884f1872d64ad5efcae286a724c5c125949216af8ee3483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>3T3-L1 Cells</topic><topic>Adipocytes - drug effects</topic><topic>Adipocytes - metabolism</topic><topic>Androstadienes - pharmacology</topic><topic>Animals</topic><topic>Cell Differentiation</topic><topic>Centrifugation, Density Gradient</topic><topic>Electroporation</topic><topic>Fluorescent Antibody Technique</topic><topic>Glucose Transporter Type 4 - metabolism</topic><topic>Glutathione Transferase - genetics</topic><topic>Insulin - pharmacology</topic><topic>Magnesium - pharmacology</topic><topic>Mice</topic><topic>Molecular biology</topic><topic>Phosphatidylinositol 3-Kinases - antagonists & inhibitors</topic><topic>Proteins</topic><topic>Recombinant Fusion Proteins</topic><topic>rho GTP-Binding Proteins - genetics</topic><topic>rho GTP-Binding Proteins - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chiang, Shian‐Huey</creatorcontrib><creatorcontrib>Chang, Louise</creatorcontrib><creatorcontrib>Saltiel, Alan R.</creatorcontrib><collection>ProQuest Ebook Central - Book Chapters - Demo use only</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Methods in Enzymology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chiang, Shian‐Huey</au><au>Chang, Louise</au><au>Saltiel, Alan R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TC10 and Insulin‐Stimulated Glucose Transport</atitle><jtitle>Methods in Enzymology</jtitle><addtitle>Methods Enzymol</addtitle><date>2006</date><risdate>2006</risdate><volume>406</volume><spage>701</spage><epage>714</epage><pages>701-714</pages><issn>0076-6879</issn><eissn>1557-7988</eissn><isbn>9780121828110</isbn><isbn>0121828115</isbn><eisbn>0080479391</eisbn><eisbn>9780080479392</eisbn><abstract>Insulin stimulates glucose uptake in insulin‐responsive tissues by means of the translocation of the glucose transporter GLUT4 from intracellular sites to the plasma membrane. Two pathways are required, one involving activation of a phosphatidylinositol 3‐kinase (PI 3‐kinase) and downstream protein kinases, and one involving activation of the Rho‐family GTPase TC10. TC10 activation by insulin is catalyzed by the exchange factor C3G, which is translocated to lipid rafts along with its binding partner CrkII as a consequence of Cbl tyrosine phosphorylation by the insulin receptor. This activation of TC10 is dependent on localization of TC10 in the lipid raft subdomains of the plasma membrane. We describe experimental approaches using the insulin‐responsive cell line 3T3‐L1 adipocytes to study the role of TC10 in insulin‐stimulated glucose transport.</abstract><cop>United States</cop><pub>Elsevier Science & Technology</pub><pmid>16472699</pmid><doi>10.1016/S0076-6879(06)06055-1</doi><oclcid>808614858</oclcid><tpages>14</tpages></addata></record> |
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| subjects | 3T3-L1 Cells Adipocytes - drug effects Adipocytes - metabolism Androstadienes - pharmacology Animals Cell Differentiation Centrifugation, Density Gradient Electroporation Fluorescent Antibody Technique Glucose Transporter Type 4 - metabolism Glutathione Transferase - genetics Insulin - pharmacology Magnesium - pharmacology Mice Molecular biology Phosphatidylinositol 3-Kinases - antagonists & inhibitors Proteins Recombinant Fusion Proteins rho GTP-Binding Proteins - genetics rho GTP-Binding Proteins - physiology |
| title | TC10 and Insulin‐Stimulated Glucose Transport |
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