SHP-2 regulates myogenesis by coupling to FAK signaling pathway
Transient dephosphorylation of FAK at Tyr-397 is required for cell cycle withdrawal early on during myogenesis. Here, we show that upon serum starvation of C2C12 myoblasts, FAK is transiently dephosphorylated in parallel with SHP-2 activation and association with FAK. SHP-2 knockdown by RNA interfer...
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| Veröffentlicht in: | FEBS letters 2009-09, Vol.583 (18), p.2975-2981 |
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| creator | de Oliveira, Michel V. Marin, Talita M. Clemente, Carolina F. Costa, Ana Paula Dalla Judice, Carla C. Franchini, Kleber G. |
| description | Transient dephosphorylation of FAK at Tyr-397 is required for cell cycle withdrawal early on during myogenesis. Here, we show that upon serum starvation of C2C12 myoblasts, FAK is transiently dephosphorylated in parallel with SHP-2 activation and association with FAK. SHP-2 knockdown by RNA interference suppressed the transient upregulation of SHP-2 and dephosphorylation of FAK during myogenesis. Furthermore, depletion of SHP-2 retarded the cell cycle withdrawal and the differentiation of serum-starved myoblasts into myotubes. These data provide a mechanistic basis for the reduction in FAK activity in differentiating myoblasts, indicating that myogenesis is critically triggered by FAK/SHP-2 complex.
MINT-
7258938:
Fak1 (uniprotkb:
P34152)
physically interacts (MI:
0915) with
shp2 (uniprotkb:
P35235) by
anti bait coimmunoprecipitation (MI:
0006) |
| doi_str_mv | 10.1016/j.febslet.2009.08.022 |
| format | Article |
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7258938:
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P34152)
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0915) with
shp2 (uniprotkb:
P35235) by
anti bait coimmunoprecipitation (MI:
0006)</description><subject>Animals</subject><subject>Cell Cycle</subject><subject>Cell Differentiation</subject><subject>Cell proliferation</subject><subject>Cell signaling</subject><subject>Cells, Cultured</subject><subject>Focal Adhesion Kinase 1 - metabolism</subject><subject>Mice</subject><subject>Muscle Development</subject><subject>Muscle, Skeletal - cytology</subject><subject>Myoblasts - cytology</subject><subject>Myogenesis</subject><subject>Phosphorylation</subject><subject>Protein Tyrosine Phosphatase, Non-Receptor Type 11 - physiology</subject><subject>Signal Transduction</subject><subject>Skeletal muscle</subject><subject>Up-Regulation</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9PwzAMxSMEYuPPRwD1xK3FaZo0PSGYNoaYBNLgHKWpOzJ17Whapn57WjaJI5wsW-89Wz8TckUhoEDF7TrIMXUFNkEIkAQgAwjDIzKmMmY-i4Q8JmMAGvk8TtiInDm3hr6XNDklI5qIRMaUj8ndcv7qh16Nq7bQDTpv01UrLNFZ56WdZ6p2W9hy5TWVN7t_9pxdlfpnsNXNx053F-Qk14XDy0M9J--z6dtk7i9eHp8m9wvf8IhyP86lyWQeC81zKVOW8kwLyFkIIeNCII8kk2g4QJTmOmGaslSHUUoFZjSTmp2Tm33utq4-W3SN2lhnsCh0iVXrlIgFpyBEL-R7oakr52rM1ba2G113ioIayKm1OpBTAzkFUvXket_1YUGbbjD7dR1Q9YL5XrCzBXb_S1Wz6UO4HN4wfAESAMGSIepuH4U9sS-LtXLGYmkwszWaRmWV_ePab17Zlnw</recordid><startdate>20090917</startdate><enddate>20090917</enddate><creator>de Oliveira, Michel V.</creator><creator>Marin, Talita M.</creator><creator>Clemente, Carolina F.</creator><creator>Costa, Ana Paula Dalla</creator><creator>Judice, Carla C.</creator><creator>Franchini, Kleber G.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090917</creationdate><title>SHP-2 regulates myogenesis by coupling to FAK signaling pathway</title><author>de Oliveira, Michel V. ; Marin, Talita M. ; Clemente, Carolina F. ; Costa, Ana Paula Dalla ; Judice, Carla C. ; Franchini, Kleber G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5415-7f8cd8f76a5f88b3b5da60f32023566e54838ec5004bfa93a13ba24b16ed1d8a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Cell Cycle</topic><topic>Cell Differentiation</topic><topic>Cell proliferation</topic><topic>Cell signaling</topic><topic>Cells, Cultured</topic><topic>Focal Adhesion Kinase 1 - metabolism</topic><topic>Mice</topic><topic>Muscle Development</topic><topic>Muscle, Skeletal - cytology</topic><topic>Myoblasts - cytology</topic><topic>Myogenesis</topic><topic>Phosphorylation</topic><topic>Protein Tyrosine Phosphatase, Non-Receptor Type 11 - physiology</topic><topic>Signal Transduction</topic><topic>Skeletal muscle</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Oliveira, Michel V.</creatorcontrib><creatorcontrib>Marin, Talita M.</creatorcontrib><creatorcontrib>Clemente, Carolina F.</creatorcontrib><creatorcontrib>Costa, Ana Paula Dalla</creatorcontrib><creatorcontrib>Judice, Carla C.</creatorcontrib><creatorcontrib>Franchini, Kleber G.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Oliveira, Michel V.</au><au>Marin, Talita M.</au><au>Clemente, Carolina F.</au><au>Costa, Ana Paula Dalla</au><au>Judice, Carla C.</au><au>Franchini, Kleber G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SHP-2 regulates myogenesis by coupling to FAK signaling pathway</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2009-09-17</date><risdate>2009</risdate><volume>583</volume><issue>18</issue><spage>2975</spage><epage>2981</epage><pages>2975-2981</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Transient dephosphorylation of FAK at Tyr-397 is required for cell cycle withdrawal early on during myogenesis. Here, we show that upon serum starvation of C2C12 myoblasts, FAK is transiently dephosphorylated in parallel with SHP-2 activation and association with FAK. SHP-2 knockdown by RNA interference suppressed the transient upregulation of SHP-2 and dephosphorylation of FAK during myogenesis. Furthermore, depletion of SHP-2 retarded the cell cycle withdrawal and the differentiation of serum-starved myoblasts into myotubes. These data provide a mechanistic basis for the reduction in FAK activity in differentiating myoblasts, indicating that myogenesis is critically triggered by FAK/SHP-2 complex.
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7258938:
Fak1 (uniprotkb:
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| source | Wiley Free Content; MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Cell Cycle Cell Differentiation Cell proliferation Cell signaling Cells, Cultured Focal Adhesion Kinase 1 - metabolism Mice Muscle Development Muscle, Skeletal - cytology Myoblasts - cytology Myogenesis Phosphorylation Protein Tyrosine Phosphatase, Non-Receptor Type 11 - physiology Signal Transduction Skeletal muscle Up-Regulation |
| title | SHP-2 regulates myogenesis by coupling to FAK signaling pathway |
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