Germ line transformation of the olive fly Bactrocera oleae using a versatile transgenesis marker
The olive fruit fly (olive fly) Bactrocera oleae (Dacus), recently introduced in North America, is the most destructive pest of olives worldwide. The lack of an efficient gene transfer technology for olive fly has hampered molecular analysis, as well as development of genetic techniques for its cont...
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| Veröffentlicht in: | Insect molecular biology 2006-02, Vol.15 (1), p.95-103 |
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| creator | Koukidou, M Klinakis, A Reboulakis, C Zagoraiou, L Tavernarakis, N Livadaras, I Economopoulos, A Savakis, C |
| description | The olive fruit fly (olive fly) Bactrocera oleae (Dacus), recently introduced in North America, is the most destructive pest of olives worldwide. The lack of an efficient gene transfer technology for olive fly has hampered molecular analysis, as well as development of genetic techniques for its control. We have developed a Minos-based transposon vector carrying a self-activating cassette which overexpresses the enhanced green fluorescent protein (EGFP). Efficient transposase-mediated integration of one to multiple copies of this vector was achieved in the germ line of B. oleae by coinjecting the vector along with in vitro synthesized Minos transposase mRNA into preblastoderm embryos. The self-activating gene construct combined with transposase mRNA present a system with potential for transgenesis of very diverse species. |
| doi_str_mv | 10.1111/j.1365-2583.2006.00613.x |
| format | Article |
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The lack of an efficient gene transfer technology for olive fly has hampered molecular analysis, as well as development of genetic techniques for its control. We have developed a Minos-based transposon vector carrying a self-activating cassette which overexpresses the enhanced green fluorescent protein (EGFP). Efficient transposase-mediated integration of one to multiple copies of this vector was achieved in the germ line of B. oleae by coinjecting the vector along with in vitro synthesized Minos transposase mRNA into preblastoderm embryos. 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The lack of an efficient gene transfer technology for olive fly has hampered molecular analysis, as well as development of genetic techniques for its control. We have developed a Minos-based transposon vector carrying a self-activating cassette which overexpresses the enhanced green fluorescent protein (EGFP). Efficient transposase-mediated integration of one to multiple copies of this vector was achieved in the germ line of B. oleae by coinjecting the vector along with in vitro synthesized Minos transposase mRNA into preblastoderm embryos. The self-activating gene construct combined with transposase mRNA present a system with potential for transgenesis of very diverse species.</description><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>Bactrocera oleae</subject><subject>Base Sequence</subject><subject>Biolistics - methods</subject><subject>Blotting, Southern</subject><subject>Dacus</subject><subject>DNA, Recombinant</subject><subject>embryo (animal)</subject><subject>Female</subject><subject>fruit flies</subject><subject>gene overexpression</subject><subject>Genetic Markers</subject><subject>genetic transformation</subject><subject>genetic vectors</subject><subject>germ line transformation</subject><subject>green fluorescent protein</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>injection</subject><subject>Male</subject><subject>messenger RNA</subject><subject>Molecular Sequence Data</subject><subject>Olea</subject><subject>Olive fruit fly</subject><subject>Tephritidae - embryology</subject><subject>Tephritidae - genetics</subject><subject>Transformation, Genetic</subject><subject>transgenesis marker</subject><subject>transgenic insects</subject><subject>transposase</subject><subject>Transposases - genetics</subject><subject>transposons</subject><issn>0962-1075</issn><issn>1365-2583</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkVFv0zAUhS0EYmXwF8DigbeEazt2bIkXNrFuUgEhNrE347g3JSVNNjsZ7b-fS6oh8QKWLFv2d4507iGEMshZWm_XORNKZlxqkXMAlafNRL59RGYPH4_JDIziGYNSHpFnMa4BQBtlnpIjpgploBQz8n2OYUPbpkM6BNfFug8bNzR9R_uaDj-Q9m1zh7Rud_TE-SH0HoNLj-iQjrHpVtTROwwxadqDxQo7jE2kGxd-YnhOntSujfjicB6Tq7MPl6fn2eLz_OL0_SLzhVYi8wbVUjrnhXRS11JzWTEOknPQhai4qirBmBAKDJaAS18g1rz0kulSeFeKY_Jm8r0J_e2IcbCbJnpsW9dhP0arypRZ8n-DzBhVMg0JfP0XuO7H0KUQlkNRcqNBJkhPkA99jAFrexOalHxnGdh9V3Zt95XYfSV235X93ZXdJunLg_9YbXD5R3goJwHvJuBXmu3uv43txceTdEnybJI3ccDtgzy1koYhSmm_fZrbazhTX64Xhb1M_KuJr11v3So00V595cAEMJCaGSnuAdVgueE</recordid><startdate>200602</startdate><enddate>200602</enddate><creator>Koukidou, M</creator><creator>Klinakis, A</creator><creator>Reboulakis, C</creator><creator>Zagoraiou, L</creator><creator>Tavernarakis, N</creator><creator>Livadaras, I</creator><creator>Economopoulos, A</creator><creator>Savakis, C</creator><general>Oxford, UK : Blackwell Science Ltd</general><general>Blackwell Science Ltd</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>200602</creationdate><title>Germ line transformation of the olive fly Bactrocera oleae using a versatile transgenesis marker</title><author>Koukidou, M ; Klinakis, A ; Reboulakis, C ; Zagoraiou, L ; Tavernarakis, N ; Livadaras, I ; Economopoulos, A ; Savakis, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4863-c9e6d5aac35a58f5825b1205220843b26bb31133609e70edc4eef27c51873ca73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Animals, Genetically Modified</topic><topic>Bactrocera oleae</topic><topic>Base Sequence</topic><topic>Biolistics - methods</topic><topic>Blotting, Southern</topic><topic>Dacus</topic><topic>DNA, Recombinant</topic><topic>embryo (animal)</topic><topic>Female</topic><topic>fruit flies</topic><topic>gene overexpression</topic><topic>Genetic Markers</topic><topic>genetic transformation</topic><topic>genetic vectors</topic><topic>germ line transformation</topic><topic>green fluorescent protein</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>injection</topic><topic>Male</topic><topic>messenger RNA</topic><topic>Molecular Sequence Data</topic><topic>Olea</topic><topic>Olive fruit fly</topic><topic>Tephritidae - embryology</topic><topic>Tephritidae - genetics</topic><topic>Transformation, Genetic</topic><topic>transgenesis marker</topic><topic>transgenic insects</topic><topic>transposase</topic><topic>Transposases - genetics</topic><topic>transposons</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koukidou, M</creatorcontrib><creatorcontrib>Klinakis, A</creatorcontrib><creatorcontrib>Reboulakis, C</creatorcontrib><creatorcontrib>Zagoraiou, L</creatorcontrib><creatorcontrib>Tavernarakis, N</creatorcontrib><creatorcontrib>Livadaras, I</creatorcontrib><creatorcontrib>Economopoulos, A</creatorcontrib><creatorcontrib>Savakis, C</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Insect molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koukidou, M</au><au>Klinakis, A</au><au>Reboulakis, C</au><au>Zagoraiou, L</au><au>Tavernarakis, N</au><au>Livadaras, I</au><au>Economopoulos, A</au><au>Savakis, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Germ line transformation of the olive fly Bactrocera oleae using a versatile transgenesis marker</atitle><jtitle>Insect molecular biology</jtitle><addtitle>Insect Mol Biol</addtitle><date>2006-02</date><risdate>2006</risdate><volume>15</volume><issue>1</issue><spage>95</spage><epage>103</epage><pages>95-103</pages><issn>0962-1075</issn><eissn>1365-2583</eissn><abstract>The olive fruit fly (olive fly) Bactrocera oleae (Dacus), recently introduced in North America, is the most destructive pest of olives worldwide. The lack of an efficient gene transfer technology for olive fly has hampered molecular analysis, as well as development of genetic techniques for its control. We have developed a Minos-based transposon vector carrying a self-activating cassette which overexpresses the enhanced green fluorescent protein (EGFP). Efficient transposase-mediated integration of one to multiple copies of this vector was achieved in the germ line of B. oleae by coinjecting the vector along with in vitro synthesized Minos transposase mRNA into preblastoderm embryos. The self-activating gene construct combined with transposase mRNA present a system with potential for transgenesis of very diverse species.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Science Ltd</pub><pmid>16469073</pmid><doi>10.1111/j.1365-2583.2006.00613.x</doi><tpages>9</tpages></addata></record> |
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| ispartof | Insect molecular biology, 2006-02, Vol.15 (1), p.95-103 |
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| subjects | Animals Animals, Genetically Modified Bactrocera oleae Base Sequence Biolistics - methods Blotting, Southern Dacus DNA, Recombinant embryo (animal) Female fruit flies gene overexpression Genetic Markers genetic transformation genetic vectors germ line transformation green fluorescent protein Green Fluorescent Proteins - genetics HeLa Cells Humans injection Male messenger RNA Molecular Sequence Data Olea Olive fruit fly Tephritidae - embryology Tephritidae - genetics Transformation, Genetic transgenesis marker transgenic insects transposase Transposases - genetics transposons |
| title | Germ line transformation of the olive fly Bactrocera oleae using a versatile transgenesis marker |
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