Telomerase from yeast Saccharomyces cerevisiae is active in vitro as a monomer
A system for isolation of yeast telomerase via RNA affinity tag in TLC1 RNA was developed. Streptavidin aptamer was inserted at two different positions in TLC1 RNA. Telomerase with TLC1 RNA with one of these inserts is functional in vivo and can be isolated by affinity chromatography in vitro. A tel...
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| Veröffentlicht in: | Biochemistry (Moscow) 2009-07, Vol.74 (7), p.749-755 |
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| container_title | Biochemistry (Moscow) |
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| creator | Shcherbakova, D. M Sokolov, K. A Zvereva, M. I Dontsova, O. A |
| description | A system for isolation of yeast telomerase via RNA affinity tag in TLC1 RNA was developed. Streptavidin aptamer was inserted at two different positions in TLC1 RNA. Telomerase with TLC1 RNA with one of these inserts is functional in vivo and can be isolated by affinity chromatography in vitro. A telomerase preparation isolated using this technique from a strain producing two distinguishable TLC1 RNA molecules (with and without aptameric insertion) resulted in isolation of active telomerase containing only TLC1 RNA with the aptamer. Our results indicate that yeast telomerase is active in vitro as a monomer. |
| doi_str_mv | 10.1134/S0006297909070074 |
| format | Article |
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M ; Sokolov, K. A ; Zvereva, M. I ; Dontsova, O. A</creator><creatorcontrib>Shcherbakova, D. M ; Sokolov, K. A ; Zvereva, M. I ; Dontsova, O. A</creatorcontrib><description>A system for isolation of yeast telomerase via RNA affinity tag in TLC1 RNA was developed. Streptavidin aptamer was inserted at two different positions in TLC1 RNA. Telomerase with TLC1 RNA with one of these inserts is functional in vivo and can be isolated by affinity chromatography in vitro. A telomerase preparation isolated using this technique from a strain producing two distinguishable TLC1 RNA molecules (with and without aptameric insertion) resulted in isolation of active telomerase containing only TLC1 RNA with the aptamer. 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M</creatorcontrib><creatorcontrib>Sokolov, K. A</creatorcontrib><creatorcontrib>Zvereva, M. I</creatorcontrib><creatorcontrib>Dontsova, O. A</creatorcontrib><title>Telomerase from yeast Saccharomyces cerevisiae is active in vitro as a monomer</title><title>Biochemistry (Moscow)</title><addtitle>Biochemistry Moscow</addtitle><addtitle>Biochemistry (Mosc)</addtitle><description>A system for isolation of yeast telomerase via RNA affinity tag in TLC1 RNA was developed. Streptavidin aptamer was inserted at two different positions in TLC1 RNA. Telomerase with TLC1 RNA with one of these inserts is functional in vivo and can be isolated by affinity chromatography in vitro. A telomerase preparation isolated using this technique from a strain producing two distinguishable TLC1 RNA molecules (with and without aptameric insertion) resulted in isolation of active telomerase containing only TLC1 RNA with the aptamer. 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| subjects | Affinity chromatography Aptamers Base Sequence Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Enzymes Life Sciences Microbiology Molecular Sequence Data Monomers Ribonucleic acid RNA RNA, Fungal - chemistry RNA, Fungal - genetics Saccharomyces cerevisiae Saccharomyces cerevisiae - chemistry Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins - chemistry Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - isolation & purification Saccharomyces cerevisiae Proteins - metabolism streptavidin Telomerase Telomerase - chemistry Telomerase - genetics Telomerase - isolation & purification Telomerase - metabolism Yeast Yeasts |
| title | Telomerase from yeast Saccharomyces cerevisiae is active in vitro as a monomer |
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