An enzyme-coupled continuous spectrophotometric assay for magnesium protoporphyrin IX methyltransferases

The second committed step in chlorophyll biosynthesis is the transfer of a methyl group from S-adenosyl- l-methionine (SAM) to magnesium protoporphyrin IX (MgP) forming MgP monomethylester (MgPME). This reaction is catalyzed by the enzyme MgP methyltransferase (ChlM). Previous investigation of this...

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Veröffentlicht in:	Analytical biochemistry 2009-11, Vol.394 (2), p.223-228
Hauptverfasser:	McLean, Samantha, Hunter, C. Neil
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenine - chemistry Adenine - metabolism Adenine deaminase Aminohydrolases - metabolism Biological Assay Cyanobacteria Cyanobacteria - enzymology Escherichia coli - genetics Escherichia coli - metabolism Hypoxanthine - chemistry Hypoxanthine - metabolism Kinetics Magnesium protoporphyrin IX (MgP) Magnesium protoporphyrin IX methyltransferase (ChlM) Magnesium protoporphyrin IX monomethylester (MgPME) Methyltransferases - chemistry Methyltransferases - genetics Methyltransferases - isolation & purification Methyltransferases - metabolism Molecular Structure Protoporphyrins - genetics Protoporphyrins - metabolism Recombinant Proteins - chemistry Recombinant Proteins - metabolism S-Adenosyl- l-homocysteine (SAH) S-Adenosyl- l-homocysteine nucleosidase S-Adenosyl- l-methionine (SAM) S-Adenosylhomocysteine - chemistry S-Adenosylhomocysteine - metabolism S-Adenosylmethionine - metabolism Spectrophotometry Substrate Specificity Synechocystis Synechocystis - enzymology Transformation, Bacterial
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