Enhancing sucrose synthase activity in transgenic potato (Solanum tuberosum L.) tubers results in increased levels of starch, ADPglucose and UDPglucose and total yield
Sucrose synthase (SuSy) is a highly regulated cytosolic enzyme that catalyzes the conversion of sucrose and a nucleoside diphosphate into the corresponding nucleoside diphosphate glucose and fructose. To determine the impact of SuSy activity in starch metabolism and yield in potato (Solanum tuberosu...
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| Veröffentlicht in: | Plant and cell physiology 2009-09, Vol.50 (9), p.1651-1662 |
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| creator | Baroja-Fernandez, E.(Instituto de Agrobiotecnologia, Nafarroa (Spain)) Munoz, F.J Montero, M Etxeberria, E Sesma, M.T Ovecka, M Bahaji, A Ezquer, I Li, J Prat, S Pozueta-Romero, J |
| description | Sucrose synthase (SuSy) is a highly regulated cytosolic enzyme that catalyzes the conversion of sucrose and a nucleoside diphosphate into the corresponding nucleoside diphosphate glucose and fructose. To determine the impact of SuSy activity in starch metabolism and yield in potato (Solanum tuberosum L.) tubers we measured sugar levels and enzyme activities in tubers of SuSy-overexpressing potato plants grown in greenhouse and open field conditions. We also transcriptionally characterized tubers of SuSy-overexpressing and -antisensed potato plants. SuSy-overexpressing tubers exhibited a substantial increase in starch, UDPglucose and ADPglucose content when compared with controls. Tuber dry weight, starch content per plant and total yield of SuSy-overexpressing tubers increased significantly over those of control plants. In contrast, activities of enzymes directly involved in starch metabolism in SuSy-overexpressing tubers were normal when compared with controls. Transcriptomic analyses using POCI arrays and the MapMan software revealed that changes in SuSy activity affect the expression of genes involved in multiple biological processes, but not that of genes directly involved in starch metabolism. These analyses also revealed a reverse correlation between the expressions of acid invertase and SuSy-encoding genes, indicating that the balance between SuSy- and acid invertase-mediated sucrolytic pathways is a major determinant of starch accumulation in potato tubers. Results presented in this work show that SuSy strongly determines the intracellular levels of UDPglucose, ADPglucose and starch, and total yield in potato tubers. We also show that enhancement of SuSy activity represents a useful strategy for increasing starch accumulation and yield in potato tubers. |
| doi_str_mv | 10.1093/pcp/pcp108 |
| format | Article |
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To determine the impact of SuSy activity in starch metabolism and yield in potato (Solanum tuberosum L.) tubers we measured sugar levels and enzyme activities in tubers of SuSy-overexpressing potato plants grown in greenhouse and open field conditions. We also transcriptionally characterized tubers of SuSy-overexpressing and -antisensed potato plants. SuSy-overexpressing tubers exhibited a substantial increase in starch, UDPglucose and ADPglucose content when compared with controls. Tuber dry weight, starch content per plant and total yield of SuSy-overexpressing tubers increased significantly over those of control plants. In contrast, activities of enzymes directly involved in starch metabolism in SuSy-overexpressing tubers were normal when compared with controls. Transcriptomic analyses using POCI arrays and the MapMan software revealed that changes in SuSy activity affect the expression of genes involved in multiple biological processes, but not that of genes directly involved in starch metabolism. These analyses also revealed a reverse correlation between the expressions of acid invertase and SuSy-encoding genes, indicating that the balance between SuSy- and acid invertase-mediated sucrolytic pathways is a major determinant of starch accumulation in potato tubers. Results presented in this work show that SuSy strongly determines the intracellular levels of UDPglucose, ADPglucose and starch, and total yield in potato tubers. We also show that enhancement of SuSy activity represents a useful strategy for increasing starch accumulation and yield in potato tubers.</description><identifier>ISSN: 0032-0781</identifier><identifier>EISSN: 1471-9053</identifier><identifier>DOI: 10.1093/pcp/pcp108</identifier><identifier>PMID: 19608713</identifier><language>eng</language><publisher>Japan: Oxford University Press</publisher><subject>ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; ALMIDON ; AMIDON ; BIOSINTESIS ; BIOSYNTHESE ; BIOSYNTHESIS ; Carbohydrate metabolism ; CROP YIELD ; ENZYME ACTIVITY ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Glucose - analysis ; Glucose - biosynthesis ; Glucosyltransferases - genetics ; Glucosyltransferases - metabolism ; Oligonucleotide Array Sequence Analysis ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Tubers - enzymology ; Plant Tubers - genetics ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; Plants, Genetically Modified - enzymology ; Plants, Genetically Modified - genetics ; RENDEMENT DES CULTURES ; RENDIMIENTO DE CULTIVOS ; RNA, Plant - genetics ; SACCHAROSE ; SOLANUM TUBEROSUM ; Solanum tuberosum - enzymology ; Solanum tuberosum - genetics ; STARCH ; Starch - analysis ; Starch - biosynthesis ; SUCROSA ; SUCROSE ; TRANSGENIC PLANTS</subject><ispartof>Plant and cell physiology, 2009-09, Vol.50 (9), p.1651-1662</ispartof><rights>The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org 2009</rights><rights>The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c525t-4a5d84e555daad71603a392ce4017083586713272cc544d341dedd6654188e1a3</citedby><cites>FETCH-LOGICAL-c525t-4a5d84e555daad71603a392ce4017083586713272cc544d341dedd6654188e1a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1584,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19608713$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baroja-Fernandez, E.(Instituto de Agrobiotecnologia, Nafarroa (Spain))</creatorcontrib><creatorcontrib>Munoz, F.J</creatorcontrib><creatorcontrib>Montero, M</creatorcontrib><creatorcontrib>Etxeberria, E</creatorcontrib><creatorcontrib>Sesma, M.T</creatorcontrib><creatorcontrib>Ovecka, M</creatorcontrib><creatorcontrib>Bahaji, A</creatorcontrib><creatorcontrib>Ezquer, I</creatorcontrib><creatorcontrib>Li, J</creatorcontrib><creatorcontrib>Prat, S</creatorcontrib><creatorcontrib>Pozueta-Romero, J</creatorcontrib><title>Enhancing sucrose synthase activity in transgenic potato (Solanum tuberosum L.) tubers results in increased levels of starch, ADPglucose and UDPglucose and total yield</title><title>Plant and cell physiology</title><addtitle>Plant Cell Physiol</addtitle><description>Sucrose synthase (SuSy) is a highly regulated cytosolic enzyme that catalyzes the conversion of sucrose and a nucleoside diphosphate into the corresponding nucleoside diphosphate glucose and fructose. To determine the impact of SuSy activity in starch metabolism and yield in potato (Solanum tuberosum L.) tubers we measured sugar levels and enzyme activities in tubers of SuSy-overexpressing potato plants grown in greenhouse and open field conditions. We also transcriptionally characterized tubers of SuSy-overexpressing and -antisensed potato plants. SuSy-overexpressing tubers exhibited a substantial increase in starch, UDPglucose and ADPglucose content when compared with controls. Tuber dry weight, starch content per plant and total yield of SuSy-overexpressing tubers increased significantly over those of control plants. In contrast, activities of enzymes directly involved in starch metabolism in SuSy-overexpressing tubers were normal when compared with controls. Transcriptomic analyses using POCI arrays and the MapMan software revealed that changes in SuSy activity affect the expression of genes involved in multiple biological processes, but not that of genes directly involved in starch metabolism. These analyses also revealed a reverse correlation between the expressions of acid invertase and SuSy-encoding genes, indicating that the balance between SuSy- and acid invertase-mediated sucrolytic pathways is a major determinant of starch accumulation in potato tubers. Results presented in this work show that SuSy strongly determines the intracellular levels of UDPglucose, ADPglucose and starch, and total yield in potato tubers. We also show that enhancement of SuSy activity represents a useful strategy for increasing starch accumulation and yield in potato tubers.</description><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>ALMIDON</subject><subject>AMIDON</subject><subject>BIOSINTESIS</subject><subject>BIOSYNTHESE</subject><subject>BIOSYNTHESIS</subject><subject>Carbohydrate metabolism</subject><subject>CROP YIELD</subject><subject>ENZYME ACTIVITY</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant</subject><subject>Glucose - analysis</subject><subject>Glucose - biosynthesis</subject><subject>Glucosyltransferases - genetics</subject><subject>Glucosyltransferases - metabolism</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Tubers - enzymology</subject><subject>Plant Tubers - genetics</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>Plants, Genetically Modified - enzymology</subject><subject>Plants, Genetically Modified - genetics</subject><subject>RENDEMENT DES CULTURES</subject><subject>RENDIMIENTO DE CULTIVOS</subject><subject>RNA, Plant - genetics</subject><subject>SACCHAROSE</subject><subject>SOLANUM TUBEROSUM</subject><subject>Solanum tuberosum - enzymology</subject><subject>Solanum tuberosum - genetics</subject><subject>STARCH</subject><subject>Starch - analysis</subject><subject>Starch - biosynthesis</subject><subject>SUCROSA</subject><subject>SUCROSE</subject><subject>TRANSGENIC PLANTS</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9rFDEUxQdR7Lb64rsSBEXFqTfJJDPz2K7VVRYs1IL0JaRJdjd1NjPmT3E_kV_TDLMo-qAPIefC757L4RTFIwzHGFr6ZlDD-DA0d4oZrmpctsDo3WIGQEkJdYMPisMQbgCypnC_OMAth6bGdFb8OHMb6ZR1axSS8n0wKOxc3MgspIr21sYdsg5FL11YG2cVGvooY49eXPSddGmLYro2eTGr5fHLaQrIm5C6GMZV65Q32U-jztyaLqB-hUKUXm1eo5O35-suqfGsdBpd_jnGfKlDO2s6_aC4t5JdMA_3_1Fx-e7s83xRLj-9_zA_WZaKERbLSjLdVIYxpqXUNeZAJW2JMhXgGhrKGp5jk5ooxapK0wprozXnrMJNY7CkR8XzyXfw_bdkQhRbG5TpclTTpyB4zStScfZfkORmAHOSwad_gTd98i6HyAxmvAWCM_RqgsYKgjcrMXi7lX4nMIixZJELFlPJGX6yd0zXW6N_o_tWM_BsAvo0_NuonDgbovn-i5T-a85JayYWX67EnC_wKYcrcZH5xxO_kr2Qa2-D-HhOAFoAzklNfwLDKsjE</recordid><startdate>20090901</startdate><enddate>20090901</enddate><creator>Baroja-Fernandez, E.(Instituto de Agrobiotecnologia, Nafarroa (Spain))</creator><creator>Munoz, F.J</creator><creator>Montero, M</creator><creator>Etxeberria, E</creator><creator>Sesma, M.T</creator><creator>Ovecka, M</creator><creator>Bahaji, A</creator><creator>Ezquer, I</creator><creator>Li, J</creator><creator>Prat, S</creator><creator>Pozueta-Romero, J</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7QP</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20090901</creationdate><title>Enhancing sucrose synthase activity in transgenic potato (Solanum tuberosum L.) tubers results in increased levels of starch, ADPglucose and UDPglucose and total yield</title><author>Baroja-Fernandez, E.(Instituto de Agrobiotecnologia, Nafarroa (Spain)) ; Munoz, F.J ; Montero, M ; Etxeberria, E ; Sesma, M.T ; Ovecka, M ; Bahaji, A ; Ezquer, I ; Li, J ; Prat, S ; Pozueta-Romero, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c525t-4a5d84e555daad71603a392ce4017083586713272cc544d341dedd6654188e1a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>ALMIDON</topic><topic>AMIDON</topic><topic>BIOSINTESIS</topic><topic>BIOSYNTHESE</topic><topic>BIOSYNTHESIS</topic><topic>Carbohydrate metabolism</topic><topic>CROP YIELD</topic><topic>ENZYME ACTIVITY</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant</topic><topic>Glucose - analysis</topic><topic>Glucose - biosynthesis</topic><topic>Glucosyltransferases - genetics</topic><topic>Glucosyltransferases - metabolism</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Tubers - enzymology</topic><topic>Plant Tubers - genetics</topic><topic>PLANTAS TRANSGENICAS</topic><topic>PLANTE TRANSGENIQUE</topic><topic>Plants, Genetically Modified - enzymology</topic><topic>Plants, Genetically Modified - genetics</topic><topic>RENDEMENT DES CULTURES</topic><topic>RENDIMIENTO DE CULTIVOS</topic><topic>RNA, Plant - genetics</topic><topic>SACCHAROSE</topic><topic>SOLANUM TUBEROSUM</topic><topic>Solanum tuberosum - enzymology</topic><topic>Solanum tuberosum - genetics</topic><topic>STARCH</topic><topic>Starch - analysis</topic><topic>Starch - biosynthesis</topic><topic>SUCROSA</topic><topic>SUCROSE</topic><topic>TRANSGENIC PLANTS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baroja-Fernandez, E.(Instituto de Agrobiotecnologia, Nafarroa (Spain))</creatorcontrib><creatorcontrib>Munoz, F.J</creatorcontrib><creatorcontrib>Montero, M</creatorcontrib><creatorcontrib>Etxeberria, E</creatorcontrib><creatorcontrib>Sesma, M.T</creatorcontrib><creatorcontrib>Ovecka, M</creatorcontrib><creatorcontrib>Bahaji, A</creatorcontrib><creatorcontrib>Ezquer, I</creatorcontrib><creatorcontrib>Li, J</creatorcontrib><creatorcontrib>Prat, S</creatorcontrib><creatorcontrib>Pozueta-Romero, J</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant and cell physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baroja-Fernandez, E.(Instituto de Agrobiotecnologia, Nafarroa (Spain))</au><au>Munoz, F.J</au><au>Montero, M</au><au>Etxeberria, E</au><au>Sesma, M.T</au><au>Ovecka, M</au><au>Bahaji, A</au><au>Ezquer, I</au><au>Li, J</au><au>Prat, S</au><au>Pozueta-Romero, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhancing sucrose synthase activity in transgenic potato (Solanum tuberosum L.) tubers results in increased levels of starch, ADPglucose and UDPglucose and total yield</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>2009-09-01</date><risdate>2009</risdate><volume>50</volume><issue>9</issue><spage>1651</spage><epage>1662</epage><pages>1651-1662</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><abstract>Sucrose synthase (SuSy) is a highly regulated cytosolic enzyme that catalyzes the conversion of sucrose and a nucleoside diphosphate into the corresponding nucleoside diphosphate glucose and fructose. To determine the impact of SuSy activity in starch metabolism and yield in potato (Solanum tuberosum L.) tubers we measured sugar levels and enzyme activities in tubers of SuSy-overexpressing potato plants grown in greenhouse and open field conditions. We also transcriptionally characterized tubers of SuSy-overexpressing and -antisensed potato plants. SuSy-overexpressing tubers exhibited a substantial increase in starch, UDPglucose and ADPglucose content when compared with controls. Tuber dry weight, starch content per plant and total yield of SuSy-overexpressing tubers increased significantly over those of control plants. In contrast, activities of enzymes directly involved in starch metabolism in SuSy-overexpressing tubers were normal when compared with controls. Transcriptomic analyses using POCI arrays and the MapMan software revealed that changes in SuSy activity affect the expression of genes involved in multiple biological processes, but not that of genes directly involved in starch metabolism. These analyses also revealed a reverse correlation between the expressions of acid invertase and SuSy-encoding genes, indicating that the balance between SuSy- and acid invertase-mediated sucrolytic pathways is a major determinant of starch accumulation in potato tubers. Results presented in this work show that SuSy strongly determines the intracellular levels of UDPglucose, ADPglucose and starch, and total yield in potato tubers. We also show that enhancement of SuSy activity represents a useful strategy for increasing starch accumulation and yield in potato tubers.</abstract><cop>Japan</cop><pub>Oxford University Press</pub><pmid>19608713</pmid><doi>10.1093/pcp/pcp108</doi><tpages>12</tpages></addata></record> |
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| ispartof | Plant and cell physiology, 2009-09, Vol.50 (9), p.1651-1662 |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE ALMIDON AMIDON BIOSINTESIS BIOSYNTHESE BIOSYNTHESIS Carbohydrate metabolism CROP YIELD ENZYME ACTIVITY Gene Expression Profiling Gene Expression Regulation, Plant Glucose - analysis Glucose - biosynthesis Glucosyltransferases - genetics Glucosyltransferases - metabolism Oligonucleotide Array Sequence Analysis Plant Proteins - genetics Plant Proteins - metabolism Plant Tubers - enzymology Plant Tubers - genetics PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants, Genetically Modified - enzymology Plants, Genetically Modified - genetics RENDEMENT DES CULTURES RENDIMIENTO DE CULTIVOS RNA, Plant - genetics SACCHAROSE SOLANUM TUBEROSUM Solanum tuberosum - enzymology Solanum tuberosum - genetics STARCH Starch - analysis Starch - biosynthesis SUCROSA SUCROSE TRANSGENIC PLANTS |
| title | Enhancing sucrose synthase activity in transgenic potato (Solanum tuberosum L.) tubers results in increased levels of starch, ADPglucose and UDPglucose and total yield |
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