Relationship Between Amphiregulin and Airway Inflammation in Children With Asthma and Eosinophilic Bronchitis
Background: Amphiregulin, a member of the epidermal growth factor family, has been shown to promote the growth of fibroblasts, to be associated with the T-helper type 2 cell adaptive immune response, and to up-regulate mucin gene expression. We aimed to determine whether sputum amphiregulin is expre...
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| Veröffentlicht in: | Chest 2009-09, Vol.136 (3), p.805-810 |
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| creator | Kim, Kyung Won Jee, Hye Mi Park, Yeo Hoon Choi, Bong Seok Sohn, Myung Hyun Kim, Kyu-Earn |
| description | Background: Amphiregulin, a member of the epidermal growth factor family, has been shown to promote the growth of fibroblasts, to be
associated with the T-helper type 2 cell adaptive immune response, and to up-regulate mucin gene expression. We aimed to determine
whether sputum amphiregulin is expressed at elevated levels in patients with asthma or eosinophilic bronchitis (EB), and whether
it is associated with eosinophilic inflammation, pulmonary function, and bronchial hyperresponsiveness in children.
Methods: A total of 117 children with asthma, 77 with EB, and 84 control subjects were enrolled in this study. Amphiregulin and eosinophil
cationic protein (ECP) levels were measured in sputum supernatants. We performed pulmonary function and methacholine challenge
tests while measuring total eosinophil count, and serum levels of total IgE and ECP in all children.
Results: The children with asthma had significantly higher levels of sputum amphiregulin (mean, 10.80 pg/mL; range, 4.07 to 38.75
pg/mL) than both the children with EB (mean, 5.76 pg/mL; range, 0.61 to 21.65 pg/mL; p = 0.013) and the control subjects (mean,
6.56 pg/mL; range, 0.51 to 17.98 pg/mL; p = 0.003). Sputum amphiregulin levels positively correlated with levels of sputum
eosinophils (γ = 0.221; p = 0.007) and sputum ECP (γ = 0.601; p < 0.0001). Negative significant correlations were found between
sputum amphiregulin and FEV 1 (γ = â0.181; p = 0.006) or post-bronchodilator therapy FEV 1 (γ = â0.233; p = 0.002). In children with asthma who were not receiving any controller medications, sputum amphiregulin level
was negatively correlated with the provocative concentration of methacholine causing a 20% fall in FEV 1 ( r = â0.398; p = 0.008).
Conclusions: Our findings suggest that childhood asthma is associated with sputum amphiregulin, whereas EB is not, and that sputum amphiregulin
would be a supportive marker of airway inflammation in asthma. |
| doi_str_mv | 10.1378/chest.08-2972 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67642071</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67642071</sourcerecordid><originalsourceid>FETCH-LOGICAL-c361t-263cec8500e4549985641da8c902249cbcb25fa071df2e97ba9e3fcb63f727173</originalsourceid><addsrcrecordid>eNpFkEtLxDAURoMoOj6WbqUb3VXzaJNmOQ6-QBBEcRnSNDWRJB2TlmH-vXFm0NXlcs_3XTgAnCN4jQhrbpTRabyGTYk5w3tghjhBJakrsg9mECJcEsrxEThO6QvmHXF6CI4QryrGMZwB_6qdHO0QkrHL4laPK61DMfdLY6P-nJwNhQxdMbdxJdfFU-id9H4TKPJpYazrYg582NEU8zQaLzf83ZBsGHKJs6q4jUNQxo42nYKDXrqkz3bzBLzf370tHsvnl4enxfy5VISiscSUKK2aGkJd1RXnTU0r1MlGcYhxxVWrWlz3EjLU9Vhz1kquSa9aSnqGGWLkBFxte5dx-J6yIOFtUto5GfQwJUEZrXCOZ7DcgioOKUXdi2W0Xsa1QFD8-hUbvwI24tdv5i92xVPrdfdP74Rm4HIHyKSk66MMyqY_DiOOEKlp5m62nLGfZpVdi-Slc7mWbF9-DVMM0iFCBRENrMkPnwWWJQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67642071</pqid></control><display><type>article</type><title>Relationship Between Amphiregulin and Airway Inflammation in Children With Asthma and Eosinophilic Bronchitis</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Kim, Kyung Won ; Jee, Hye Mi ; Park, Yeo Hoon ; Choi, Bong Seok ; Sohn, Myung Hyun ; Kim, Kyu-Earn</creator><creatorcontrib>Kim, Kyung Won ; Jee, Hye Mi ; Park, Yeo Hoon ; Choi, Bong Seok ; Sohn, Myung Hyun ; Kim, Kyu-Earn</creatorcontrib><description>Background: Amphiregulin, a member of the epidermal growth factor family, has been shown to promote the growth of fibroblasts, to be
associated with the T-helper type 2 cell adaptive immune response, and to up-regulate mucin gene expression. We aimed to determine
whether sputum amphiregulin is expressed at elevated levels in patients with asthma or eosinophilic bronchitis (EB), and whether
it is associated with eosinophilic inflammation, pulmonary function, and bronchial hyperresponsiveness in children.
Methods: A total of 117 children with asthma, 77 with EB, and 84 control subjects were enrolled in this study. Amphiregulin and eosinophil
cationic protein (ECP) levels were measured in sputum supernatants. We performed pulmonary function and methacholine challenge
tests while measuring total eosinophil count, and serum levels of total IgE and ECP in all children.
Results: The children with asthma had significantly higher levels of sputum amphiregulin (mean, 10.80 pg/mL; range, 4.07 to 38.75
pg/mL) than both the children with EB (mean, 5.76 pg/mL; range, 0.61 to 21.65 pg/mL; p = 0.013) and the control subjects (mean,
6.56 pg/mL; range, 0.51 to 17.98 pg/mL; p = 0.003). Sputum amphiregulin levels positively correlated with levels of sputum
eosinophils (γ = 0.221; p = 0.007) and sputum ECP (γ = 0.601; p < 0.0001). Negative significant correlations were found between
sputum amphiregulin and FEV 1 (γ = â0.181; p = 0.006) or post-bronchodilator therapy FEV 1 (γ = â0.233; p = 0.002). In children with asthma who were not receiving any controller medications, sputum amphiregulin level
was negatively correlated with the provocative concentration of methacholine causing a 20% fall in FEV 1 ( r = â0.398; p = 0.008).
Conclusions: Our findings suggest that childhood asthma is associated with sputum amphiregulin, whereas EB is not, and that sputum amphiregulin
would be a supportive marker of airway inflammation in asthma.</description><identifier>ISSN: 0012-3692</identifier><identifier>EISSN: 1931-3543</identifier><identifier>DOI: 10.1378/chest.08-2972</identifier><identifier>PMID: 19447920</identifier><identifier>CODEN: CHETBF</identifier><language>eng</language><publisher>Northbrook, IL: American College of Chest Physicians</publisher><subject>Amphiregulin ; Analysis of Variance ; Asthma - metabolism ; Biological and medical sciences ; Bronchial Hyperreactivity - metabolism ; Bronchial Provocation Tests ; Bronchitis - metabolism ; Cardiology. Vascular system ; Case-Control Studies ; Child ; Chronic obstructive pulmonary disease, asthma ; EGF Family of Proteins ; Enzyme-Linked Immunosorbent Assay ; Eosinophils ; Female ; Glycoproteins - metabolism ; Humans ; Immunoglobulin E - blood ; Inflammation - metabolism ; Intercellular Signaling Peptides and Proteins - metabolism ; Leukocyte Count ; Male ; Medical sciences ; Methacholine Chloride ; Mucins - genetics ; Mucins - metabolism ; Pneumology ; Prospective Studies ; Sputum - chemistry ; Statistics, Nonparametric ; Up-Regulation</subject><ispartof>Chest, 2009-09, Vol.136 (3), p.805-810</ispartof><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-263cec8500e4549985641da8c902249cbcb25fa071df2e97ba9e3fcb63f727173</citedby><cites>FETCH-LOGICAL-c361t-263cec8500e4549985641da8c902249cbcb25fa071df2e97ba9e3fcb63f727173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21911356$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19447920$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Kyung Won</creatorcontrib><creatorcontrib>Jee, Hye Mi</creatorcontrib><creatorcontrib>Park, Yeo Hoon</creatorcontrib><creatorcontrib>Choi, Bong Seok</creatorcontrib><creatorcontrib>Sohn, Myung Hyun</creatorcontrib><creatorcontrib>Kim, Kyu-Earn</creatorcontrib><title>Relationship Between Amphiregulin and Airway Inflammation in Children With Asthma and Eosinophilic Bronchitis</title><title>Chest</title><addtitle>Chest</addtitle><description>Background: Amphiregulin, a member of the epidermal growth factor family, has been shown to promote the growth of fibroblasts, to be
associated with the T-helper type 2 cell adaptive immune response, and to up-regulate mucin gene expression. We aimed to determine
whether sputum amphiregulin is expressed at elevated levels in patients with asthma or eosinophilic bronchitis (EB), and whether
it is associated with eosinophilic inflammation, pulmonary function, and bronchial hyperresponsiveness in children.
Methods: A total of 117 children with asthma, 77 with EB, and 84 control subjects were enrolled in this study. Amphiregulin and eosinophil
cationic protein (ECP) levels were measured in sputum supernatants. We performed pulmonary function and methacholine challenge
tests while measuring total eosinophil count, and serum levels of total IgE and ECP in all children.
Results: The children with asthma had significantly higher levels of sputum amphiregulin (mean, 10.80 pg/mL; range, 4.07 to 38.75
pg/mL) than both the children with EB (mean, 5.76 pg/mL; range, 0.61 to 21.65 pg/mL; p = 0.013) and the control subjects (mean,
6.56 pg/mL; range, 0.51 to 17.98 pg/mL; p = 0.003). Sputum amphiregulin levels positively correlated with levels of sputum
eosinophils (γ = 0.221; p = 0.007) and sputum ECP (γ = 0.601; p < 0.0001). Negative significant correlations were found between
sputum amphiregulin and FEV 1 (γ = â0.181; p = 0.006) or post-bronchodilator therapy FEV 1 (γ = â0.233; p = 0.002). In children with asthma who were not receiving any controller medications, sputum amphiregulin level
was negatively correlated with the provocative concentration of methacholine causing a 20% fall in FEV 1 ( r = â0.398; p = 0.008).
Conclusions: Our findings suggest that childhood asthma is associated with sputum amphiregulin, whereas EB is not, and that sputum amphiregulin
would be a supportive marker of airway inflammation in asthma.</description><subject>Amphiregulin</subject><subject>Analysis of Variance</subject><subject>Asthma - metabolism</subject><subject>Biological and medical sciences</subject><subject>Bronchial Hyperreactivity - metabolism</subject><subject>Bronchial Provocation Tests</subject><subject>Bronchitis - metabolism</subject><subject>Cardiology. Vascular system</subject><subject>Case-Control Studies</subject><subject>Child</subject><subject>Chronic obstructive pulmonary disease, asthma</subject><subject>EGF Family of Proteins</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Eosinophils</subject><subject>Female</subject><subject>Glycoproteins - metabolism</subject><subject>Humans</subject><subject>Immunoglobulin E - blood</subject><subject>Inflammation - metabolism</subject><subject>Intercellular Signaling Peptides and Proteins - metabolism</subject><subject>Leukocyte Count</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Methacholine Chloride</subject><subject>Mucins - genetics</subject><subject>Mucins - metabolism</subject><subject>Pneumology</subject><subject>Prospective Studies</subject><subject>Sputum - chemistry</subject><subject>Statistics, Nonparametric</subject><subject>Up-Regulation</subject><issn>0012-3692</issn><issn>1931-3543</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtLxDAURoMoOj6WbqUb3VXzaJNmOQ6-QBBEcRnSNDWRJB2TlmH-vXFm0NXlcs_3XTgAnCN4jQhrbpTRabyGTYk5w3tghjhBJakrsg9mECJcEsrxEThO6QvmHXF6CI4QryrGMZwB_6qdHO0QkrHL4laPK61DMfdLY6P-nJwNhQxdMbdxJdfFU-id9H4TKPJpYazrYg582NEU8zQaLzf83ZBsGHKJs6q4jUNQxo42nYKDXrqkz3bzBLzf370tHsvnl4enxfy5VISiscSUKK2aGkJd1RXnTU0r1MlGcYhxxVWrWlz3EjLU9Vhz1kquSa9aSnqGGWLkBFxte5dx-J6yIOFtUto5GfQwJUEZrXCOZ7DcgioOKUXdi2W0Xsa1QFD8-hUbvwI24tdv5i92xVPrdfdP74Rm4HIHyKSk66MMyqY_DiOOEKlp5m62nLGfZpVdi-Slc7mWbF9-DVMM0iFCBRENrMkPnwWWJQ</recordid><startdate>20090901</startdate><enddate>20090901</enddate><creator>Kim, Kyung Won</creator><creator>Jee, Hye Mi</creator><creator>Park, Yeo Hoon</creator><creator>Choi, Bong Seok</creator><creator>Sohn, Myung Hyun</creator><creator>Kim, Kyu-Earn</creator><general>American College of Chest Physicians</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090901</creationdate><title>Relationship Between Amphiregulin and Airway Inflammation in Children With Asthma and Eosinophilic Bronchitis</title><author>Kim, Kyung Won ; Jee, Hye Mi ; Park, Yeo Hoon ; Choi, Bong Seok ; Sohn, Myung Hyun ; Kim, Kyu-Earn</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-263cec8500e4549985641da8c902249cbcb25fa071df2e97ba9e3fcb63f727173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Amphiregulin</topic><topic>Analysis of Variance</topic><topic>Asthma - metabolism</topic><topic>Biological and medical sciences</topic><topic>Bronchial Hyperreactivity - metabolism</topic><topic>Bronchial Provocation Tests</topic><topic>Bronchitis - metabolism</topic><topic>Cardiology. Vascular system</topic><topic>Case-Control Studies</topic><topic>Child</topic><topic>Chronic obstructive pulmonary disease, asthma</topic><topic>EGF Family of Proteins</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Eosinophils</topic><topic>Female</topic><topic>Glycoproteins - metabolism</topic><topic>Humans</topic><topic>Immunoglobulin E - blood</topic><topic>Inflammation - metabolism</topic><topic>Intercellular Signaling Peptides and Proteins - metabolism</topic><topic>Leukocyte Count</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Methacholine Chloride</topic><topic>Mucins - genetics</topic><topic>Mucins - metabolism</topic><topic>Pneumology</topic><topic>Prospective Studies</topic><topic>Sputum - chemistry</topic><topic>Statistics, Nonparametric</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Kyung Won</creatorcontrib><creatorcontrib>Jee, Hye Mi</creatorcontrib><creatorcontrib>Park, Yeo Hoon</creatorcontrib><creatorcontrib>Choi, Bong Seok</creatorcontrib><creatorcontrib>Sohn, Myung Hyun</creatorcontrib><creatorcontrib>Kim, Kyu-Earn</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chest</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Kyung Won</au><au>Jee, Hye Mi</au><au>Park, Yeo Hoon</au><au>Choi, Bong Seok</au><au>Sohn, Myung Hyun</au><au>Kim, Kyu-Earn</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relationship Between Amphiregulin and Airway Inflammation in Children With Asthma and Eosinophilic Bronchitis</atitle><jtitle>Chest</jtitle><addtitle>Chest</addtitle><date>2009-09-01</date><risdate>2009</risdate><volume>136</volume><issue>3</issue><spage>805</spage><epage>810</epage><pages>805-810</pages><issn>0012-3692</issn><eissn>1931-3543</eissn><coden>CHETBF</coden><abstract>Background: Amphiregulin, a member of the epidermal growth factor family, has been shown to promote the growth of fibroblasts, to be
associated with the T-helper type 2 cell adaptive immune response, and to up-regulate mucin gene expression. We aimed to determine
whether sputum amphiregulin is expressed at elevated levels in patients with asthma or eosinophilic bronchitis (EB), and whether
it is associated with eosinophilic inflammation, pulmonary function, and bronchial hyperresponsiveness in children.
Methods: A total of 117 children with asthma, 77 with EB, and 84 control subjects were enrolled in this study. Amphiregulin and eosinophil
cationic protein (ECP) levels were measured in sputum supernatants. We performed pulmonary function and methacholine challenge
tests while measuring total eosinophil count, and serum levels of total IgE and ECP in all children.
Results: The children with asthma had significantly higher levels of sputum amphiregulin (mean, 10.80 pg/mL; range, 4.07 to 38.75
pg/mL) than both the children with EB (mean, 5.76 pg/mL; range, 0.61 to 21.65 pg/mL; p = 0.013) and the control subjects (mean,
6.56 pg/mL; range, 0.51 to 17.98 pg/mL; p = 0.003). Sputum amphiregulin levels positively correlated with levels of sputum
eosinophils (γ = 0.221; p = 0.007) and sputum ECP (γ = 0.601; p < 0.0001). Negative significant correlations were found between
sputum amphiregulin and FEV 1 (γ = â0.181; p = 0.006) or post-bronchodilator therapy FEV 1 (γ = â0.233; p = 0.002). In children with asthma who were not receiving any controller medications, sputum amphiregulin level
was negatively correlated with the provocative concentration of methacholine causing a 20% fall in FEV 1 ( r = â0.398; p = 0.008).
Conclusions: Our findings suggest that childhood asthma is associated with sputum amphiregulin, whereas EB is not, and that sputum amphiregulin
would be a supportive marker of airway inflammation in asthma.</abstract><cop>Northbrook, IL</cop><pub>American College of Chest Physicians</pub><pmid>19447920</pmid><doi>10.1378/chest.08-2972</doi><tpages>6</tpages></addata></record> |
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| subjects | Amphiregulin Analysis of Variance Asthma - metabolism Biological and medical sciences Bronchial Hyperreactivity - metabolism Bronchial Provocation Tests Bronchitis - metabolism Cardiology. Vascular system Case-Control Studies Child Chronic obstructive pulmonary disease, asthma EGF Family of Proteins Enzyme-Linked Immunosorbent Assay Eosinophils Female Glycoproteins - metabolism Humans Immunoglobulin E - blood Inflammation - metabolism Intercellular Signaling Peptides and Proteins - metabolism Leukocyte Count Male Medical sciences Methacholine Chloride Mucins - genetics Mucins - metabolism Pneumology Prospective Studies Sputum - chemistry Statistics, Nonparametric Up-Regulation |
| title | Relationship Between Amphiregulin and Airway Inflammation in Children With Asthma and Eosinophilic Bronchitis |
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