Acute Effects of Testosterone Infusion on the Serum Luteinizing Hormone Profile in Eumenorrheic and Polycystic Ovary Syndrome Adolescents
Context: Little is known about the neuroendocrine effects of androgens on the GnRH-LH unit in females. Objective: Our objective was to evaluate androgen negative feedback on the GnRH-LH axis in eumenorrheic and polycystic ovary syndrome (PCOS) adolescents. Design and Setting: We conducted a prospect...
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| Veröffentlicht in: | The journal of clinical endocrinology and metabolism 2009-09, Vol.94 (9), p.3602-3610 |
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| container_title | The journal of clinical endocrinology and metabolism |
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| creator | Ropelato, María Gabriela García Rudaz, María Cecilia Escobar, María Eugenia Bengolea, Sonia Viviana Calcagno, María Luján Veldhuis, Johannes D. Barontini, Marta |
| description | Context: Little is known about the neuroendocrine effects of androgens on the GnRH-LH unit in females.
Objective: Our objective was to evaluate androgen negative feedback on the GnRH-LH axis in eumenorrheic and polycystic ovary syndrome (PCOS) adolescents.
Design and Setting: We conducted a prospective, longitudinal, randomized, double-blind study at a pediatric endocrinology clinical research center.
Participants: Seven nonobese PCOS adolescents and seven matched controls (C) were studied in the early follicular phase of three consecutive menstrual cycles or in three consecutive months.
Intervention: Pulsatile LH release was determined during saline [baseline (B)] and constant testosterone (T) infusions: low dose (T-LD) 0.75 and high dose (T-HD) 2.5 mg/12 h iv. Blood samples were drawn every 20 min overnight.
Main Outcome Measures: LH (immunofluorometric assay) and T (electrochemiluminescence immunoassay) were determined at B, and during both T-LD and T-HD. LH profiles were analyzed by deconvolution and approximate entropy analyses.
Results: On T-LD, C and PCOS serum T levels increased 2- to 3-fold vs. B. On T-HD, T values doubled in both groups vs. T-LD. Controls on T-LD had greater 12-h pulsatile LH secretion rate (P < 0.05 vs. B) and on T-HD had lower mean, pulsatile, basal LH release and LH approximate entropy (vs. B, P < 0.05). PCOS did not respond to T-LD. High-dose T did not alter mean LH in PCOS but increased pulsatile and reduced basal LH secretion.
Conclusions: PCOS adolescents have impaired suppression of pulsatile LH secretion rate consistent with reduced androgen negative feedback. Attenuation of T feedback in nonobese adolescents with PCOS extends the pathophysiology of this syndrome.
PCOS adolescents have impaired suppression of pulsatile luteinizing hormone secretion under high dose of testosterone infused overnight, which is consistent with reduced pituitary sensitivity to androgen. negative feedback. |
| doi_str_mv | 10.1210/jc.2009-0402 |
| format | Article |
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Objective: Our objective was to evaluate androgen negative feedback on the GnRH-LH axis in eumenorrheic and polycystic ovary syndrome (PCOS) adolescents.
Design and Setting: We conducted a prospective, longitudinal, randomized, double-blind study at a pediatric endocrinology clinical research center.
Participants: Seven nonobese PCOS adolescents and seven matched controls (C) were studied in the early follicular phase of three consecutive menstrual cycles or in three consecutive months.
Intervention: Pulsatile LH release was determined during saline [baseline (B)] and constant testosterone (T) infusions: low dose (T-LD) 0.75 and high dose (T-HD) 2.5 mg/12 h iv. Blood samples were drawn every 20 min overnight.
Main Outcome Measures: LH (immunofluorometric assay) and T (electrochemiluminescence immunoassay) were determined at B, and during both T-LD and T-HD. LH profiles were analyzed by deconvolution and approximate entropy analyses.
Results: On T-LD, C and PCOS serum T levels increased 2- to 3-fold vs. B. On T-HD, T values doubled in both groups vs. T-LD. Controls on T-LD had greater 12-h pulsatile LH secretion rate (P < 0.05 vs. B) and on T-HD had lower mean, pulsatile, basal LH release and LH approximate entropy (vs. B, P < 0.05). PCOS did not respond to T-LD. High-dose T did not alter mean LH in PCOS but increased pulsatile and reduced basal LH secretion.
Conclusions: PCOS adolescents have impaired suppression of pulsatile LH secretion rate consistent with reduced androgen negative feedback. Attenuation of T feedback in nonobese adolescents with PCOS extends the pathophysiology of this syndrome.
PCOS adolescents have impaired suppression of pulsatile luteinizing hormone secretion under high dose of testosterone infused overnight, which is consistent with reduced pituitary sensitivity to androgen. negative feedback.</description><identifier>ISSN: 0021-972X</identifier><identifier>EISSN: 1945-7197</identifier><identifier>DOI: 10.1210/jc.2009-0402</identifier><identifier>PMID: 19567528</identifier><identifier>CODEN: JCEMAZ</identifier><language>eng</language><publisher>Bethesda, MD: Endocrine Society</publisher><subject>Adolescent ; Biological and medical sciences ; Double-Blind Method ; Endocrinopathies ; Feedback, Physiological ; Feeding. Feeding behavior ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Longitudinal Studies ; Luteinizing Hormone - blood ; Luteinizing Hormone - secretion ; Medical sciences ; Polycystic Ovary Syndrome - metabolism ; Prospective Studies ; Testosterone - blood ; Testosterone - pharmacology ; Vertebrates: anatomy and physiology, studies on body, several organs or systems ; Vertebrates: endocrinology</subject><ispartof>The journal of clinical endocrinology and metabolism, 2009-09, Vol.94 (9), p.3602-3610</ispartof><rights>Copyright © 2009 by The Endocrine Society</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5126-b29879c330f9e752f3e2db798dada8701dfe58cc3ce0f2f31d5435269c0a3d033</citedby><cites>FETCH-LOGICAL-c5126-b29879c330f9e752f3e2db798dada8701dfe58cc3ce0f2f31d5435269c0a3d033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21923636$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19567528$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ropelato, María Gabriela</creatorcontrib><creatorcontrib>García Rudaz, María Cecilia</creatorcontrib><creatorcontrib>Escobar, María Eugenia</creatorcontrib><creatorcontrib>Bengolea, Sonia Viviana</creatorcontrib><creatorcontrib>Calcagno, María Luján</creatorcontrib><creatorcontrib>Veldhuis, Johannes D.</creatorcontrib><creatorcontrib>Barontini, Marta</creatorcontrib><title>Acute Effects of Testosterone Infusion on the Serum Luteinizing Hormone Profile in Eumenorrheic and Polycystic Ovary Syndrome Adolescents</title><title>The journal of clinical endocrinology and metabolism</title><addtitle>J Clin Endocrinol Metab</addtitle><description>Context: Little is known about the neuroendocrine effects of androgens on the GnRH-LH unit in females.
Objective: Our objective was to evaluate androgen negative feedback on the GnRH-LH axis in eumenorrheic and polycystic ovary syndrome (PCOS) adolescents.
Design and Setting: We conducted a prospective, longitudinal, randomized, double-blind study at a pediatric endocrinology clinical research center.
Participants: Seven nonobese PCOS adolescents and seven matched controls (C) were studied in the early follicular phase of three consecutive menstrual cycles or in three consecutive months.
Intervention: Pulsatile LH release was determined during saline [baseline (B)] and constant testosterone (T) infusions: low dose (T-LD) 0.75 and high dose (T-HD) 2.5 mg/12 h iv. Blood samples were drawn every 20 min overnight.
Main Outcome Measures: LH (immunofluorometric assay) and T (electrochemiluminescence immunoassay) were determined at B, and during both T-LD and T-HD. LH profiles were analyzed by deconvolution and approximate entropy analyses.
Results: On T-LD, C and PCOS serum T levels increased 2- to 3-fold vs. B. On T-HD, T values doubled in both groups vs. T-LD. Controls on T-LD had greater 12-h pulsatile LH secretion rate (P < 0.05 vs. B) and on T-HD had lower mean, pulsatile, basal LH release and LH approximate entropy (vs. B, P < 0.05). PCOS did not respond to T-LD. High-dose T did not alter mean LH in PCOS but increased pulsatile and reduced basal LH secretion.
Conclusions: PCOS adolescents have impaired suppression of pulsatile LH secretion rate consistent with reduced androgen negative feedback. Attenuation of T feedback in nonobese adolescents with PCOS extends the pathophysiology of this syndrome.
PCOS adolescents have impaired suppression of pulsatile luteinizing hormone secretion under high dose of testosterone infused overnight, which is consistent with reduced pituitary sensitivity to androgen. negative feedback.</description><subject>Adolescent</subject><subject>Biological and medical sciences</subject><subject>Double-Blind Method</subject><subject>Endocrinopathies</subject><subject>Feedback, Physiological</subject><subject>Feeding. Feeding behavior</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Longitudinal Studies</subject><subject>Luteinizing Hormone - blood</subject><subject>Luteinizing Hormone - secretion</subject><subject>Medical sciences</subject><subject>Polycystic Ovary Syndrome - metabolism</subject><subject>Prospective Studies</subject><subject>Testosterone - blood</subject><subject>Testosterone - pharmacology</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><subject>Vertebrates: endocrinology</subject><issn>0021-972X</issn><issn>1945-7197</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkU2PUyEUhonROLW6c23Y6Mo78nG_WDaT6kzSZCaZMXFHKBwslQsV7p1J_Qf-a7lpoxs5EEJ43sM5Lwi9peSSMko-7fUlI0RUpCbsGVpQUTdVR0X3HC0IYbQSHft2gV7lvCeE1nXDX6ILKpq2a1i_QL9XehoBr60FPWYcLX6APMY8QooB8E2wU3Yx4DLHHeB7SNOAN0Xigvvlwnd8HdMwk3cpWucBu4DX0wAhprQDp7EKBt9Ff9THPJbj7aNKR3x_DCbFAfDKRA9ZQxjza_TCKp_hzXlfoq-f1w9X19Xm9svN1WpT6Yayttoy0XdCc06sgNKD5cDMthO9UUb1HaHGQtNrzTUQW26paWresFZoorghnC_Rh1PeQ4o_p9KsHFypwHsVIE5Ztl3L-7qmBfx4AnWKOSew8pDcUMqXlMjZernXcrZeztYX_N0577QdwPyDz14X4P0ZUFkrb5MK2uW_HKOC8bbEEtUn7in68g35h5-eIMkdKD_uJCmjbru-ml8uQUhVVjvL-EkGwUSdXIBDgpzlPk4pFEP_X_UfGhavAQ</recordid><startdate>200909</startdate><enddate>200909</enddate><creator>Ropelato, María Gabriela</creator><creator>García Rudaz, María Cecilia</creator><creator>Escobar, María Eugenia</creator><creator>Bengolea, Sonia Viviana</creator><creator>Calcagno, María Luján</creator><creator>Veldhuis, Johannes D.</creator><creator>Barontini, Marta</creator><general>Endocrine Society</general><general>Copyright by The Endocrine Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200909</creationdate><title>Acute Effects of Testosterone Infusion on the Serum Luteinizing Hormone Profile in Eumenorrheic and Polycystic Ovary Syndrome Adolescents</title><author>Ropelato, María Gabriela ; García Rudaz, María Cecilia ; Escobar, María Eugenia ; Bengolea, Sonia Viviana ; Calcagno, María Luján ; Veldhuis, Johannes D. ; Barontini, Marta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5126-b29879c330f9e752f3e2db798dada8701dfe58cc3ce0f2f31d5435269c0a3d033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adolescent</topic><topic>Biological and medical sciences</topic><topic>Double-Blind Method</topic><topic>Endocrinopathies</topic><topic>Feedback, Physiological</topic><topic>Feeding. Feeding behavior</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Longitudinal Studies</topic><topic>Luteinizing Hormone - blood</topic><topic>Luteinizing Hormone - secretion</topic><topic>Medical sciences</topic><topic>Polycystic Ovary Syndrome - metabolism</topic><topic>Prospective Studies</topic><topic>Testosterone - blood</topic><topic>Testosterone - pharmacology</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ropelato, María Gabriela</creatorcontrib><creatorcontrib>García Rudaz, María Cecilia</creatorcontrib><creatorcontrib>Escobar, María Eugenia</creatorcontrib><creatorcontrib>Bengolea, Sonia Viviana</creatorcontrib><creatorcontrib>Calcagno, María Luján</creatorcontrib><creatorcontrib>Veldhuis, Johannes D.</creatorcontrib><creatorcontrib>Barontini, Marta</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of clinical endocrinology and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ropelato, María Gabriela</au><au>García Rudaz, María Cecilia</au><au>Escobar, María Eugenia</au><au>Bengolea, Sonia Viviana</au><au>Calcagno, María Luján</au><au>Veldhuis, Johannes D.</au><au>Barontini, Marta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acute Effects of Testosterone Infusion on the Serum Luteinizing Hormone Profile in Eumenorrheic and Polycystic Ovary Syndrome Adolescents</atitle><jtitle>The journal of clinical endocrinology and metabolism</jtitle><addtitle>J Clin Endocrinol Metab</addtitle><date>2009-09</date><risdate>2009</risdate><volume>94</volume><issue>9</issue><spage>3602</spage><epage>3610</epage><pages>3602-3610</pages><issn>0021-972X</issn><eissn>1945-7197</eissn><coden>JCEMAZ</coden><abstract>Context: Little is known about the neuroendocrine effects of androgens on the GnRH-LH unit in females.
Objective: Our objective was to evaluate androgen negative feedback on the GnRH-LH axis in eumenorrheic and polycystic ovary syndrome (PCOS) adolescents.
Design and Setting: We conducted a prospective, longitudinal, randomized, double-blind study at a pediatric endocrinology clinical research center.
Participants: Seven nonobese PCOS adolescents and seven matched controls (C) were studied in the early follicular phase of three consecutive menstrual cycles or in three consecutive months.
Intervention: Pulsatile LH release was determined during saline [baseline (B)] and constant testosterone (T) infusions: low dose (T-LD) 0.75 and high dose (T-HD) 2.5 mg/12 h iv. Blood samples were drawn every 20 min overnight.
Main Outcome Measures: LH (immunofluorometric assay) and T (electrochemiluminescence immunoassay) were determined at B, and during both T-LD and T-HD. LH profiles were analyzed by deconvolution and approximate entropy analyses.
Results: On T-LD, C and PCOS serum T levels increased 2- to 3-fold vs. B. On T-HD, T values doubled in both groups vs. T-LD. Controls on T-LD had greater 12-h pulsatile LH secretion rate (P < 0.05 vs. B) and on T-HD had lower mean, pulsatile, basal LH release and LH approximate entropy (vs. B, P < 0.05). PCOS did not respond to T-LD. High-dose T did not alter mean LH in PCOS but increased pulsatile and reduced basal LH secretion.
Conclusions: PCOS adolescents have impaired suppression of pulsatile LH secretion rate consistent with reduced androgen negative feedback. Attenuation of T feedback in nonobese adolescents with PCOS extends the pathophysiology of this syndrome.
PCOS adolescents have impaired suppression of pulsatile luteinizing hormone secretion under high dose of testosterone infused overnight, which is consistent with reduced pituitary sensitivity to androgen. negative feedback.</abstract><cop>Bethesda, MD</cop><pub>Endocrine Society</pub><pmid>19567528</pmid><doi>10.1210/jc.2009-0402</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adolescent Biological and medical sciences Double-Blind Method Endocrinopathies Feedback, Physiological Feeding. Feeding behavior Female Fundamental and applied biological sciences. Psychology Humans Longitudinal Studies Luteinizing Hormone - blood Luteinizing Hormone - secretion Medical sciences Polycystic Ovary Syndrome - metabolism Prospective Studies Testosterone - blood Testosterone - pharmacology Vertebrates: anatomy and physiology, studies on body, several organs or systems Vertebrates: endocrinology |
| title | Acute Effects of Testosterone Infusion on the Serum Luteinizing Hormone Profile in Eumenorrheic and Polycystic Ovary Syndrome Adolescents |
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