Babesia gibsoni: Identification, expression, localization, and serological characterization of a Babesia gibsoni 22-kDa protein
Babesia gibsoni causes canine babesiosis. Here, we describe the identification and characterization of a novel gene, BgP22, containing an open reading frame of 621 bp and encoding a 22-kDa protein from B. gibsoni, as a serodiagnostic candidate. The recombinant BgP22 (rBgP22) was expressed and used a...
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| Veröffentlicht in: | Experimental parasitology 2009-11, Vol.123 (3), p.273-276 |
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| creator | Goo, Youn-Kyoung Jia, Honglin Terkawi, Mohamad Alaa Aboge, Gabriel Oluga Yamagishi, Junya Nishikawa, Yoshifumi Kim, Suk Jang, Hyung-Kwan Fujisaki, Kozo Xuan, Xuenan |
| description | Babesia gibsoni causes canine babesiosis. Here, we describe the identification and characterization of a novel gene, BgP22, containing an open reading frame of 621
bp and encoding a 22-kDa protein from
B. gibsoni, as a serodiagnostic candidate. The recombinant BgP22 (rBgP22) was expressed and used as an antigen to produce anti-rBgP22 sera in mice. Using these anti-rBgP22 sera, a native 22-kDa protein was recognized by Western blot analysis and observed in the membrane of the parasites by immunofluorescent antibody tests (IFAT). The enzyme-linked immunosorbent assay (ELISA) using the rBgP22 detected specific antibodies to this protein in the sera of dogs experimentally and naturally infected with
B.
gibsoni in chronic stage. Furthermore, it did not show a cross reaction with the closely related apicomplexan parasites, indicating that the rBgP22 could be used as a diagnostic antigen for a detection of the chronic carrier stages of
B. gibsoni infection. |
| doi_str_mv | 10.1016/j.exppara.2009.07.017 |
| format | Article |
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bp and encoding a 22-kDa protein from
B. gibsoni, as a serodiagnostic candidate. The recombinant BgP22 (rBgP22) was expressed and used as an antigen to produce anti-rBgP22 sera in mice. Using these anti-rBgP22 sera, a native 22-kDa protein was recognized by Western blot analysis and observed in the membrane of the parasites by immunofluorescent antibody tests (IFAT). The enzyme-linked immunosorbent assay (ELISA) using the rBgP22 detected specific antibodies to this protein in the sera of dogs experimentally and naturally infected with
B.
gibsoni in chronic stage. Furthermore, it did not show a cross reaction with the closely related apicomplexan parasites, indicating that the rBgP22 could be used as a diagnostic antigen for a detection of the chronic carrier stages of
B. gibsoni infection.</description><identifier>ISSN: 0014-4894</identifier><identifier>EISSN: 1090-2449</identifier><identifier>DOI: 10.1016/j.exppara.2009.07.017</identifier><identifier>PMID: 19665019</identifier><identifier>CODEN: EXPAAA</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Protozoan - biosynthesis ; Antibodies, Protozoan - blood ; Antibodies, Protozoan - immunology ; antibody detection ; antigen-antibody reactions ; Antigens, Protozoan - chemistry ; Antigens, Protozoan - genetics ; Antigens, Protozoan - immunology ; Babesia - genetics ; Babesia - immunology ; Babesia gibsoni ; Babesia gibsoni 22-kDa protein ; babesiosis ; Babesiosis - diagnosis ; Babesiosis - parasitology ; Babesiosis - veterinary ; Base Sequence ; BgP22 ; BgP22 gene ; Biological and medical sciences ; complementary DNA ; Cross Reactions ; diagnostic antigens ; disease detection ; DNA, Protozoan - chemistry ; Dog Diseases - diagnosis ; Dog Diseases - parasitology ; Dogs ; ELISA ; enzyme-linked immunosorbent assay ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; gene expression ; Gene Expression Regulation ; Immune Sera - biosynthesis ; Immune Sera - immunology ; Life cycle. Host-agent relationship. Pathogenesis ; membrane proteins ; Mice ; Molecular Sequence Data ; nucleotide sequences ; Protozoa ; Protozoan Proteins - chemistry ; Protozoan Proteins - genetics ; Protozoan Proteins - immunology ; recombinant antigens ; serodiagnosis</subject><ispartof>Experimental parasitology, 2009-11, Vol.123 (3), p.273-276</ispartof><rights>2009 Elsevier Inc.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-41f19197f9255b8609da3a5b5c46fcf2fec4d1de86ff55f6814beb60c5d2ffe73</citedby><cites>FETCH-LOGICAL-c448t-41f19197f9255b8609da3a5b5c46fcf2fec4d1de86ff55f6814beb60c5d2ffe73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.exppara.2009.07.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21955561$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19665019$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Goo, Youn-Kyoung</creatorcontrib><creatorcontrib>Jia, Honglin</creatorcontrib><creatorcontrib>Terkawi, Mohamad Alaa</creatorcontrib><creatorcontrib>Aboge, Gabriel Oluga</creatorcontrib><creatorcontrib>Yamagishi, Junya</creatorcontrib><creatorcontrib>Nishikawa, Yoshifumi</creatorcontrib><creatorcontrib>Kim, Suk</creatorcontrib><creatorcontrib>Jang, Hyung-Kwan</creatorcontrib><creatorcontrib>Fujisaki, Kozo</creatorcontrib><creatorcontrib>Xuan, Xuenan</creatorcontrib><title>Babesia gibsoni: Identification, expression, localization, and serological characterization of a Babesia gibsoni 22-kDa protein</title><title>Experimental parasitology</title><addtitle>Exp Parasitol</addtitle><description>Babesia gibsoni causes canine babesiosis. Here, we describe the identification and characterization of a novel gene, BgP22, containing an open reading frame of 621
bp and encoding a 22-kDa protein from
B. gibsoni, as a serodiagnostic candidate. The recombinant BgP22 (rBgP22) was expressed and used as an antigen to produce anti-rBgP22 sera in mice. Using these anti-rBgP22 sera, a native 22-kDa protein was recognized by Western blot analysis and observed in the membrane of the parasites by immunofluorescent antibody tests (IFAT). The enzyme-linked immunosorbent assay (ELISA) using the rBgP22 detected specific antibodies to this protein in the sera of dogs experimentally and naturally infected with
B.
gibsoni in chronic stage. Furthermore, it did not show a cross reaction with the closely related apicomplexan parasites, indicating that the rBgP22 could be used as a diagnostic antigen for a detection of the chronic carrier stages of
B. gibsoni infection.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Protozoan - biosynthesis</subject><subject>Antibodies, Protozoan - blood</subject><subject>Antibodies, Protozoan - immunology</subject><subject>antibody detection</subject><subject>antigen-antibody reactions</subject><subject>Antigens, Protozoan - chemistry</subject><subject>Antigens, Protozoan - genetics</subject><subject>Antigens, Protozoan - immunology</subject><subject>Babesia - genetics</subject><subject>Babesia - immunology</subject><subject>Babesia gibsoni</subject><subject>Babesia gibsoni 22-kDa protein</subject><subject>babesiosis</subject><subject>Babesiosis - diagnosis</subject><subject>Babesiosis - parasitology</subject><subject>Babesiosis - veterinary</subject><subject>Base Sequence</subject><subject>BgP22</subject><subject>BgP22 gene</subject><subject>Biological and medical sciences</subject><subject>complementary DNA</subject><subject>Cross Reactions</subject><subject>diagnostic antigens</subject><subject>disease detection</subject><subject>DNA, Protozoan - chemistry</subject><subject>Dog Diseases - diagnosis</subject><subject>Dog Diseases - parasitology</subject><subject>Dogs</subject><subject>ELISA</subject><subject>enzyme-linked immunosorbent assay</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>Gene Expression Regulation</subject><subject>Immune Sera - biosynthesis</subject><subject>Immune Sera - immunology</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>membrane proteins</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>Protozoa</subject><subject>Protozoan Proteins - chemistry</subject><subject>Protozoan Proteins - genetics</subject><subject>Protozoan Proteins - immunology</subject><subject>recombinant antigens</subject><subject>serodiagnosis</subject><issn>0014-4894</issn><issn>1090-2449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0UFvFCEYBmBiNHat_gSVSz054wcDzODF1Gq1SRMP2jNhmI-VdXbYwqxRL_51aXeiiZeeIOGB9wsvIU8Z1AyYerWp8cduZ5OtOYCuoa2BtffIioGGiguh75MVABOV6LQ4Io9y3gBAx7h4SI6YVkoC0yvy-63tMQdL16HPcQqv6cWA0xx8cHYOcXpJS0zCnG_3Y3R2DL-WEzsNNGOKY1wXPVL3tYzjZkyLoNFTS_8LoJxX395ZuktxxjA9Jg-8HTM-WdZjcnX-_svZx-ry04eLs9PLygnRzZVgnmmmW6-5lH2nQA-2sbKXTijvPPfoxMAG7JT3UnrVMdFjr8DJgXuPbXNMXhzeLbnXe8yz2YbscBzthHGfjWpVI3XH74QcWq2AqwLlAboUc07ozS6FrU0_DQNzU5HZmKUic1ORgdaUisq9Z0vAvt_i8O_W0kkBJwuwuXyrT3ZyIf91nGkppWLFPT84b6Ox61TM1WcOrCnZXSuarog3B4HlZ78HTCa7gJPDISR0sxliuGPYPxUtvTo</recordid><startdate>20091101</startdate><enddate>20091101</enddate><creator>Goo, Youn-Kyoung</creator><creator>Jia, Honglin</creator><creator>Terkawi, Mohamad Alaa</creator><creator>Aboge, Gabriel Oluga</creator><creator>Yamagishi, Junya</creator><creator>Nishikawa, Yoshifumi</creator><creator>Kim, Suk</creator><creator>Jang, Hyung-Kwan</creator><creator>Fujisaki, Kozo</creator><creator>Xuan, Xuenan</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20091101</creationdate><title>Babesia gibsoni: Identification, expression, localization, and serological characterization of a Babesia gibsoni 22-kDa protein</title><author>Goo, Youn-Kyoung ; Jia, Honglin ; Terkawi, Mohamad Alaa ; Aboge, Gabriel Oluga ; Yamagishi, Junya ; Nishikawa, Yoshifumi ; Kim, Suk ; Jang, Hyung-Kwan ; Fujisaki, Kozo ; Xuan, Xuenan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-41f19197f9255b8609da3a5b5c46fcf2fec4d1de86ff55f6814beb60c5d2ffe73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Protozoan - biosynthesis</topic><topic>Antibodies, Protozoan - blood</topic><topic>Antibodies, Protozoan - immunology</topic><topic>antibody detection</topic><topic>antigen-antibody reactions</topic><topic>Antigens, Protozoan - chemistry</topic><topic>Antigens, Protozoan - genetics</topic><topic>Antigens, Protozoan - immunology</topic><topic>Babesia - genetics</topic><topic>Babesia - immunology</topic><topic>Babesia gibsoni</topic><topic>Babesia gibsoni 22-kDa protein</topic><topic>babesiosis</topic><topic>Babesiosis - diagnosis</topic><topic>Babesiosis - parasitology</topic><topic>Babesiosis - veterinary</topic><topic>Base Sequence</topic><topic>BgP22</topic><topic>BgP22 gene</topic><topic>Biological and medical sciences</topic><topic>complementary DNA</topic><topic>Cross Reactions</topic><topic>diagnostic antigens</topic><topic>disease detection</topic><topic>DNA, Protozoan - chemistry</topic><topic>Dog Diseases - diagnosis</topic><topic>Dog Diseases - parasitology</topic><topic>Dogs</topic><topic>ELISA</topic><topic>enzyme-linked immunosorbent assay</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>Gene Expression Regulation</topic><topic>Immune Sera - biosynthesis</topic><topic>Immune Sera - immunology</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>membrane proteins</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>Protozoa</topic><topic>Protozoan Proteins - chemistry</topic><topic>Protozoan Proteins - genetics</topic><topic>Protozoan Proteins - immunology</topic><topic>recombinant antigens</topic><topic>serodiagnosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goo, Youn-Kyoung</creatorcontrib><creatorcontrib>Jia, Honglin</creatorcontrib><creatorcontrib>Terkawi, Mohamad Alaa</creatorcontrib><creatorcontrib>Aboge, Gabriel Oluga</creatorcontrib><creatorcontrib>Yamagishi, Junya</creatorcontrib><creatorcontrib>Nishikawa, Yoshifumi</creatorcontrib><creatorcontrib>Kim, Suk</creatorcontrib><creatorcontrib>Jang, Hyung-Kwan</creatorcontrib><creatorcontrib>Fujisaki, Kozo</creatorcontrib><creatorcontrib>Xuan, Xuenan</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goo, Youn-Kyoung</au><au>Jia, Honglin</au><au>Terkawi, Mohamad Alaa</au><au>Aboge, Gabriel Oluga</au><au>Yamagishi, Junya</au><au>Nishikawa, Yoshifumi</au><au>Kim, Suk</au><au>Jang, Hyung-Kwan</au><au>Fujisaki, Kozo</au><au>Xuan, Xuenan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Babesia gibsoni: Identification, expression, localization, and serological characterization of a Babesia gibsoni 22-kDa protein</atitle><jtitle>Experimental parasitology</jtitle><addtitle>Exp Parasitol</addtitle><date>2009-11-01</date><risdate>2009</risdate><volume>123</volume><issue>3</issue><spage>273</spage><epage>276</epage><pages>273-276</pages><issn>0014-4894</issn><eissn>1090-2449</eissn><coden>EXPAAA</coden><abstract>Babesia gibsoni causes canine babesiosis. Here, we describe the identification and characterization of a novel gene, BgP22, containing an open reading frame of 621
bp and encoding a 22-kDa protein from
B. gibsoni, as a serodiagnostic candidate. The recombinant BgP22 (rBgP22) was expressed and used as an antigen to produce anti-rBgP22 sera in mice. Using these anti-rBgP22 sera, a native 22-kDa protein was recognized by Western blot analysis and observed in the membrane of the parasites by immunofluorescent antibody tests (IFAT). The enzyme-linked immunosorbent assay (ELISA) using the rBgP22 detected specific antibodies to this protein in the sera of dogs experimentally and naturally infected with
B.
gibsoni in chronic stage. Furthermore, it did not show a cross reaction with the closely related apicomplexan parasites, indicating that the rBgP22 could be used as a diagnostic antigen for a detection of the chronic carrier stages of
B. gibsoni infection.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>19665019</pmid><doi>10.1016/j.exppara.2009.07.017</doi><tpages>4</tpages></addata></record> |
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| ispartof | Experimental parasitology, 2009-11, Vol.123 (3), p.273-276 |
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| subjects | Amino Acid Sequence Animals Antibodies, Protozoan - biosynthesis Antibodies, Protozoan - blood Antibodies, Protozoan - immunology antibody detection antigen-antibody reactions Antigens, Protozoan - chemistry Antigens, Protozoan - genetics Antigens, Protozoan - immunology Babesia - genetics Babesia - immunology Babesia gibsoni Babesia gibsoni 22-kDa protein babesiosis Babesiosis - diagnosis Babesiosis - parasitology Babesiosis - veterinary Base Sequence BgP22 BgP22 gene Biological and medical sciences complementary DNA Cross Reactions diagnostic antigens disease detection DNA, Protozoan - chemistry Dog Diseases - diagnosis Dog Diseases - parasitology Dogs ELISA enzyme-linked immunosorbent assay Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology gene expression Gene Expression Regulation Immune Sera - biosynthesis Immune Sera - immunology Life cycle. Host-agent relationship. Pathogenesis membrane proteins Mice Molecular Sequence Data nucleotide sequences Protozoa Protozoan Proteins - chemistry Protozoan Proteins - genetics Protozoan Proteins - immunology recombinant antigens serodiagnosis |
| title | Babesia gibsoni: Identification, expression, localization, and serological characterization of a Babesia gibsoni 22-kDa protein |
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