Giardia canis: Ultrastructural analysis of G. canis trophozoites transfected with full length G. canis virus cDNA transcripts

Giardia canis virus (GCV) is a double-stranded RNA (dsRNA) virus of the family Totiviridae. In this study, the full length cDNA of the G. canis virus was constructed in pPoly2/sfinot vector and RNA was transcribed in vitro. Virus-free G. canis trophozoites were transfected with in vitro transcribed...

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Veröffentlicht in:	Experimental parasitology 2009-11, Vol.123 (3), p.212-217
Hauptverfasser:	Cao, Lili, Gong, Pengtao, Li, Jianhua, Zhang, Xichen, Zou, Xiaohuan, Tuo, Wenbin, Liu, Quan, Wang, Qiuyue, Zhang, Guocai, Chen, Lifeng, Li, Lingdan, Su, Libo
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Canis complementary DNA Cytoplasm DNA, Complementary - genetics Double-stranded RNA Electroporation Fundamental and applied biological sciences. Psychology General aspects Giardia Giardia - ultrastructure Giardia - virology Giardia canis Giardiavirus Giardiavirus - genetics Giardiavirus - pathogenicity Giardiavirus - physiology Giardiavirus - ultrastructure infection Life cycle. Host-agent relationship. Pathogenesis Mammalia Microbiology Microscopy, Electron, Transmission Nuclei Polymerase Chain Reaction Protozoa RNA RNA transcript RNA, Viral - genetics Totiviridae transcription (genetics) Transfection Transmission electron microscopy Trophozoites Ultrastructure Vectors Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution Virion - pathogenicity Virion - physiology Virion - ultrastructure Virology Virus Replication Virus-free
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