Specific and sensitive detection of Nosema bombi (Microsporidia: Nosematidae) in bumble bees ( Bombus spp.; Hymenoptera: Apidae) by PCR of partial rRNA gene sequences

A polymerase chain reaction (PCR) based method was developed for the specific and sensitive diagnosis of the microsporidian parasite Nosema bombi in bumble bees ( Bombus spp.). Four primer pairs, amplifying ribosomal RNA (rRNA) gene fragments, were tested on N. bombi and the related microsporidia No...

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Veröffentlicht in:	Journal of invertebrate pathology 2006-02, Vol.91 (2), p.98-104
Hauptverfasser:	Klee, Julia, Tek Tay, Wee, Paxton, Robert J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apidae Apis mellifera bee diseases Bees - microbiology Biological and medical sciences Bombus Bombus pascuorum Bombus spp Diagnostic test disease detection disease diagnosis DNA primers DNA, Fungal - analysis DNA, Fungal - genetics Fundamental and applied biological sciences. Psychology genes Genes, Fungal Genes, rRNA Hymenoptera Insecta Invertebrates Microsporidia Nosema Nosema - genetics Nosema - isolation & purification Nosema apis Nosema bombi Nosema ceranae nosema disease Nosematidae nucleotide sequences Pathology PCR polymerase chain reaction Polymerase Chain Reaction - methods ribosomal DNA Ribosomal RNA gene Sequence Analysis, DNA Species Specificity spores
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