Characterisation of non-multiresistant methicillin-resistant Staphylococcus aureus (including EMRSA-15) in Kuwait Hospitals
This study characterised non-multiresistant methicillin-resistant Staphylococcus aureus (nmMRSA) isolates from Kuwait hospitals to ascertain whether they were community-acquired MRSA (CA-MRSA). Forty-two nmMRSA isolates obtained between July 2001 and October 2003 were analysed by staphylococcal cass...
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| Veröffentlicht in: | Clinical microbiology and infection 2006-03, Vol.12 (3), p.262-269 |
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| creator | Udo, E.E. Al-Sweih, N. Noronha, B. |
| description | This study characterised non-multiresistant methicillin-resistant Staphylococcus aureus (nmMRSA) isolates from Kuwait hospitals to ascertain whether they were community-acquired MRSA (CA-MRSA). Forty-two nmMRSA isolates obtained between July 2001 and October 2003 were analysed by staphylococcal cassette chromosome mec (SCCmec) typing, bacteriophage typing, production of Panton-Valentine leukocidin (PVL), urease and staphylococcal enterotoxins A, B, C and D, TSST-1, and by pulsed-field gel electrophoresis (PFGE). Forty-one isolates were SCCmec type IV, and one isolate was SCCmec type III. The isolates belonged to six PFGE patterns, with two types, A and D, distributed in six and four hospitals, respectively. Most (n = 26; 61.9%) isolates produced urease. These isolates were mainly from wound and skin infections, showed low-level methicillin resistance (MIC 8–48 mg/L), and nine carried genes for PVL. These characteristics, together with their carriage of the type-IV SCCmec, identified the isolates as CA-MRSA. Ten of the 16 urease-negative isolates produced staphylococal enterotoxin C; 12 reacted weakly with phage 75, and were resistant to clindamycin and/or erythromycin, which are characteristics of EMRSA-15. Thus, this study identified the co-existence of two types of nmMRSA, i.e., CA-MRSA and EMRSA-15, in Kuwait hospitals. |
| doi_str_mv | 10.1111/j.1469-0691.2005.01350.x |
| format | Article |
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Forty-two nmMRSA isolates obtained between July 2001 and October 2003 were analysed by staphylococcal cassette chromosome mec (SCCmec) typing, bacteriophage typing, production of Panton-Valentine leukocidin (PVL), urease and staphylococcal enterotoxins A, B, C and D, TSST-1, and by pulsed-field gel electrophoresis (PFGE). Forty-one isolates were SCCmec type IV, and one isolate was SCCmec type III. The isolates belonged to six PFGE patterns, with two types, A and D, distributed in six and four hospitals, respectively. Most (n = 26; 61.9%) isolates produced urease. These isolates were mainly from wound and skin infections, showed low-level methicillin resistance (MIC 8–48 mg/L), and nine carried genes for PVL. These characteristics, together with their carriage of the type-IV SCCmec, identified the isolates as CA-MRSA. Ten of the 16 urease-negative isolates produced staphylococal enterotoxin C; 12 reacted weakly with phage 75, and were resistant to clindamycin and/or erythromycin, which are characteristics of EMRSA-15. Thus, this study identified the co-existence of two types of nmMRSA, i.e., CA-MRSA and EMRSA-15, in Kuwait hospitals.</description><identifier>ISSN: 1198-743X</identifier><identifier>EISSN: 1469-0691</identifier><identifier>DOI: 10.1111/j.1469-0691.2005.01350.x</identifier><identifier>PMID: 16451414</identifier><language>eng</language><publisher>Oxford, UK: Elsevier Ltd</publisher><subject>Anti-Bacterial Agents - pharmacology ; Antibacterial agents ; Antibiotic resistance ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Bacterial diseases ; Bacterial Proteins - genetics ; Bacterial Toxins - genetics ; Bacterial Typing Techniques ; beta-Lactams - pharmacology ; Biological and medical sciences ; community-acquired MRSA ; Cross Infection ; DNA, Bacterial - analysis ; Electrophoresis, Gel, Pulsed-Field ; EMRSA-15 ; Enterotoxins - metabolism ; Exotoxins - genetics ; Genes, Bacterial ; Hospitals ; Human bacterial diseases ; Humans ; Infectious diseases ; Kuwait ; Leukocidins ; Medical sciences ; Methicillin - pharmacology ; Methicillin Resistance ; Microbial Sensitivity Tests ; MRSA ; Pharmacology. Drug treatments ; Recombinases - genetics ; SCCmec ; Staphylococcal Infections - microbiology ; Staphylococcal infections, streptococcal infections, pneumococcal infections ; Staphylococcus aureus ; Staphylococcus aureus - classification ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - genetics ; Staphylococcus aureus - metabolism ; Urease - metabolism</subject><ispartof>Clinical microbiology and infection, 2006-03, Vol.12 (3), p.262-269</ispartof><rights>2006 European Society of Clinical Infectious Diseases</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5370-e5ea3670a94f3f5077f1c3eaeb50ea109b8cf3f1269791cca57eda6af47a89b53</citedby><cites>FETCH-LOGICAL-c5370-e5ea3670a94f3f5077f1c3eaeb50ea109b8cf3f1269791cca57eda6af47a89b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1469-0691.2005.01350.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1469-0691.2005.01350.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,27933,27934,45583,45584</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17481894$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16451414$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Udo, E.E.</creatorcontrib><creatorcontrib>Al-Sweih, N.</creatorcontrib><creatorcontrib>Noronha, B.</creatorcontrib><title>Characterisation of non-multiresistant methicillin-resistant Staphylococcus aureus (including EMRSA-15) in Kuwait Hospitals</title><title>Clinical microbiology and infection</title><addtitle>Clin Microbiol Infect</addtitle><description>This study characterised non-multiresistant methicillin-resistant Staphylococcus aureus (nmMRSA) isolates from Kuwait hospitals to ascertain whether they were community-acquired MRSA (CA-MRSA). Forty-two nmMRSA isolates obtained between July 2001 and October 2003 were analysed by staphylococcal cassette chromosome mec (SCCmec) typing, bacteriophage typing, production of Panton-Valentine leukocidin (PVL), urease and staphylococcal enterotoxins A, B, C and D, TSST-1, and by pulsed-field gel electrophoresis (PFGE). Forty-one isolates were SCCmec type IV, and one isolate was SCCmec type III. The isolates belonged to six PFGE patterns, with two types, A and D, distributed in six and four hospitals, respectively. Most (n = 26; 61.9%) isolates produced urease. These isolates were mainly from wound and skin infections, showed low-level methicillin resistance (MIC 8–48 mg/L), and nine carried genes for PVL. These characteristics, together with their carriage of the type-IV SCCmec, identified the isolates as CA-MRSA. Ten of the 16 urease-negative isolates produced staphylococal enterotoxin C; 12 reacted weakly with phage 75, and were resistant to clindamycin and/or erythromycin, which are characteristics of EMRSA-15. Thus, this study identified the co-existence of two types of nmMRSA, i.e., CA-MRSA and EMRSA-15, in Kuwait hospitals.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibacterial agents</subject><subject>Antibiotic resistance</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Bacterial diseases</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Toxins - genetics</subject><subject>Bacterial Typing Techniques</subject><subject>beta-Lactams - pharmacology</subject><subject>Biological and medical sciences</subject><subject>community-acquired MRSA</subject><subject>Cross Infection</subject><subject>DNA, Bacterial - analysis</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>EMRSA-15</subject><subject>Enterotoxins - metabolism</subject><subject>Exotoxins - genetics</subject><subject>Genes, Bacterial</subject><subject>Hospitals</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Kuwait</subject><subject>Leukocidins</subject><subject>Medical sciences</subject><subject>Methicillin - pharmacology</subject><subject>Methicillin Resistance</subject><subject>Microbial Sensitivity Tests</subject><subject>MRSA</subject><subject>Pharmacology. Drug treatments</subject><subject>Recombinases - genetics</subject><subject>SCCmec</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcal infections, streptococcal infections, pneumococcal infections</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - classification</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - metabolism</subject><subject>Urease - metabolism</subject><issn>1198-743X</issn><issn>1469-0691</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtv1DAURiMEog_4CygbEF0k2HFsJwsWZVQoYiokChI7645zw3iU2IPt0I748zjMiFkWb67lez6_TpbllJQ0jTebktaiLYhoaVkRwktCGSfl_aPs9F_jcZrTtilkzb6fZGchbAghFWP10-yEiprTmtan2e_FGjzoiN4EiMbZ3PW5dbYYpyEaj8GECDbmI8a10WYYjC2Oq7cRtuvd4LTTego5TB5TeW2sHqbO2B_51c2X28uC8ovc2PzTdAcm5tcubE2EITzLnvSp4PNDPc--vb_6urgulp8_fFxcLgvNmSQFcgQmJIG27lnPiZQ91QwBV5wgUNKuGp0atBKtbKnWwCV2IKCvJTTtirPz7NV-3613PycMUY0maBwGsOimoIQUleBcPAjSVkrGeZXAZg9q70Lw2KutNyP4naJEzYbURs0i1CxCzYbUX0PqPkVfHM6YViN2x-BBSQJeHgAIGobeg9UmHDlZN7RpZ-7tnrszA-7--wJqsbyZZyn_bp_H9PW_DHoVtEGrsUvadVSdMw-_5g-MEsOu</recordid><startdate>200603</startdate><enddate>200603</enddate><creator>Udo, E.E.</creator><creator>Al-Sweih, N.</creator><creator>Noronha, B.</creator><general>Elsevier Ltd</general><general>Blackwell Science Ltd</general><general>Blackwell</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200603</creationdate><title>Characterisation of non-multiresistant methicillin-resistant Staphylococcus aureus (including EMRSA-15) in Kuwait Hospitals</title><author>Udo, E.E. ; Al-Sweih, N. ; Noronha, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5370-e5ea3670a94f3f5077f1c3eaeb50ea109b8cf3f1269791cca57eda6af47a89b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibacterial agents</topic><topic>Antibiotic resistance</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Bacterial diseases</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Toxins - genetics</topic><topic>Bacterial Typing Techniques</topic><topic>beta-Lactams - pharmacology</topic><topic>Biological and medical sciences</topic><topic>community-acquired MRSA</topic><topic>Cross Infection</topic><topic>DNA, Bacterial - analysis</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>EMRSA-15</topic><topic>Enterotoxins - metabolism</topic><topic>Exotoxins - genetics</topic><topic>Genes, Bacterial</topic><topic>Hospitals</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Kuwait</topic><topic>Leukocidins</topic><topic>Medical sciences</topic><topic>Methicillin - pharmacology</topic><topic>Methicillin Resistance</topic><topic>Microbial Sensitivity Tests</topic><topic>MRSA</topic><topic>Pharmacology. Drug treatments</topic><topic>Recombinases - genetics</topic><topic>SCCmec</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcal infections, streptococcal infections, pneumococcal infections</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - classification</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - metabolism</topic><topic>Urease - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Udo, E.E.</creatorcontrib><creatorcontrib>Al-Sweih, N.</creatorcontrib><creatorcontrib>Noronha, B.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical microbiology and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Udo, E.E.</au><au>Al-Sweih, N.</au><au>Noronha, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterisation of non-multiresistant methicillin-resistant Staphylococcus aureus (including EMRSA-15) in Kuwait Hospitals</atitle><jtitle>Clinical microbiology and infection</jtitle><addtitle>Clin Microbiol Infect</addtitle><date>2006-03</date><risdate>2006</risdate><volume>12</volume><issue>3</issue><spage>262</spage><epage>269</epage><pages>262-269</pages><issn>1198-743X</issn><eissn>1469-0691</eissn><abstract>This study characterised non-multiresistant methicillin-resistant Staphylococcus aureus (nmMRSA) isolates from Kuwait hospitals to ascertain whether they were community-acquired MRSA (CA-MRSA). Forty-two nmMRSA isolates obtained between July 2001 and October 2003 were analysed by staphylococcal cassette chromosome mec (SCCmec) typing, bacteriophage typing, production of Panton-Valentine leukocidin (PVL), urease and staphylococcal enterotoxins A, B, C and D, TSST-1, and by pulsed-field gel electrophoresis (PFGE). Forty-one isolates were SCCmec type IV, and one isolate was SCCmec type III. The isolates belonged to six PFGE patterns, with two types, A and D, distributed in six and four hospitals, respectively. Most (n = 26; 61.9%) isolates produced urease. These isolates were mainly from wound and skin infections, showed low-level methicillin resistance (MIC 8–48 mg/L), and nine carried genes for PVL. These characteristics, together with their carriage of the type-IV SCCmec, identified the isolates as CA-MRSA. Ten of the 16 urease-negative isolates produced staphylococal enterotoxin C; 12 reacted weakly with phage 75, and were resistant to clindamycin and/or erythromycin, which are characteristics of EMRSA-15. Thus, this study identified the co-existence of two types of nmMRSA, i.e., CA-MRSA and EMRSA-15, in Kuwait hospitals.</abstract><cop>Oxford, UK</cop><pub>Elsevier Ltd</pub><pmid>16451414</pmid><doi>10.1111/j.1469-0691.2005.01350.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anti-Bacterial Agents - pharmacology Antibacterial agents Antibiotic resistance Antibiotics. Antiinfectious agents. Antiparasitic agents Bacterial diseases Bacterial Proteins - genetics Bacterial Toxins - genetics Bacterial Typing Techniques beta-Lactams - pharmacology Biological and medical sciences community-acquired MRSA Cross Infection DNA, Bacterial - analysis Electrophoresis, Gel, Pulsed-Field EMRSA-15 Enterotoxins - metabolism Exotoxins - genetics Genes, Bacterial Hospitals Human bacterial diseases Humans Infectious diseases Kuwait Leukocidins Medical sciences Methicillin - pharmacology Methicillin Resistance Microbial Sensitivity Tests MRSA Pharmacology. Drug treatments Recombinases - genetics SCCmec Staphylococcal Infections - microbiology Staphylococcal infections, streptococcal infections, pneumococcal infections Staphylococcus aureus Staphylococcus aureus - classification Staphylococcus aureus - drug effects Staphylococcus aureus - genetics Staphylococcus aureus - metabolism Urease - metabolism |
| title | Characterisation of non-multiresistant methicillin-resistant Staphylococcus aureus (including EMRSA-15) in Kuwait Hospitals |
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