An investigation of the molecular mechanisms contributing to high-level erythromycin resistance in Campylobacter
The molecular mechanisms contributing to high-level erythromycin resistance in Campylobacter jejuni and Campylobacter coli isolates were investigated. The A2075G mutation in the 23S rRNA target genes was identified in all high-level erythromycin-resistant isolates. A number of amino acid substitutio...
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| Veröffentlicht in: | International journal of antimicrobial agents 2006, Vol.27 (1), p.40-45 |
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| creator | Corcoran, Deborah Quinn, Teresa Cotter, Leslie Fanning, Séamus |
| description | The molecular mechanisms contributing to high-level erythromycin resistance in
Campylobacter jejuni and
Campylobacter coli isolates were investigated. The A2075G mutation in the 23S rRNA target genes was identified in all high-level erythromycin-resistant isolates. A number of amino acid substitutions together with insertions and deletions were identified in the corresponding genes encoding L4 and L22 ribosomal proteins both of resistant and susceptible isolates. Amino acid substitutions identified in the resistant strains were located outside regions known to be altered in these proteins. The efflux pump inhibitor
l-phenylalanine-
l-arginine-β-naphthylamide (PAβN) increased the susceptibility to erythromycin in one of four isolates displaying high-level erythromycin resistance, and reduced the minimal inhibitory concentration displayed by an erythromycin-susceptible
C. coli isolate. The A2075G mutation in the 23S rRNA appeared to be the main contributor to high-level erythromycin resistance in
Campylobacter. Other mutations/amino acid substitutions found in the 50S ribosomal subunit encoding proteins L4 and L22 do not appear to be linked to the high-level erythromycin-resistant phenotype. Active efflux contributes to the intrinsic resistance to erythromycin in
Campylobacter and may contribute to high-level resistance in some isolates. |
| doi_str_mv | 10.1016/j.ijantimicag.2005.08.019 |
| format | Article |
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Campylobacter jejuni and
Campylobacter coli isolates were investigated. The A2075G mutation in the 23S rRNA target genes was identified in all high-level erythromycin-resistant isolates. A number of amino acid substitutions together with insertions and deletions were identified in the corresponding genes encoding L4 and L22 ribosomal proteins both of resistant and susceptible isolates. Amino acid substitutions identified in the resistant strains were located outside regions known to be altered in these proteins. The efflux pump inhibitor
l-phenylalanine-
l-arginine-β-naphthylamide (PAβN) increased the susceptibility to erythromycin in one of four isolates displaying high-level erythromycin resistance, and reduced the minimal inhibitory concentration displayed by an erythromycin-susceptible
C. coli isolate. The A2075G mutation in the 23S rRNA appeared to be the main contributor to high-level erythromycin resistance in
Campylobacter. Other mutations/amino acid substitutions found in the 50S ribosomal subunit encoding proteins L4 and L22 do not appear to be linked to the high-level erythromycin-resistant phenotype. Active efflux contributes to the intrinsic resistance to erythromycin in
Campylobacter and may contribute to high-level resistance in some isolates.</description><identifier>ISSN: 0924-8579</identifier><identifier>EISSN: 1872-7913</identifier><identifier>DOI: 10.1016/j.ijantimicag.2005.08.019</identifier><identifier>PMID: 16318913</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Biological and medical sciences ; Campylobacter ; Campylobacter coli ; Campylobacter coli - drug effects ; Campylobacter coli - isolation & purification ; Campylobacter coli - physiology ; Campylobacter jejuni ; Campylobacter jejuni - drug effects ; Campylobacter jejuni - isolation & purification ; Campylobacter jejuni - physiology ; Drug Resistance, Bacterial - genetics ; Efflux ; Erythromycin ; Erythromycin - pharmacology ; Humans ; Medical sciences ; Microbial Sensitivity Tests ; Molecular Sequence Data ; Pharmacology. Drug treatments ; Ribosomal proteins ; Ribosomal Proteins - genetics ; RNA, Ribosomal, 23S - genetics</subject><ispartof>International journal of antimicrobial agents, 2006, Vol.27 (1), p.40-45</ispartof><rights>2005 Elsevier B.V. and the International Society of Chemotherapy</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c502t-9288e544f44725ae9e6b3deda06b4c3a42c9aa8847e8c55b4baaa8eacf635a153</citedby><cites>FETCH-LOGICAL-c502t-9288e544f44725ae9e6b3deda06b4c3a42c9aa8847e8c55b4baaa8eacf635a153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0924857905002645$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17399972$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16318913$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Corcoran, Deborah</creatorcontrib><creatorcontrib>Quinn, Teresa</creatorcontrib><creatorcontrib>Cotter, Leslie</creatorcontrib><creatorcontrib>Fanning, Séamus</creatorcontrib><title>An investigation of the molecular mechanisms contributing to high-level erythromycin resistance in Campylobacter</title><title>International journal of antimicrobial agents</title><addtitle>Int J Antimicrob Agents</addtitle><description>The molecular mechanisms contributing to high-level erythromycin resistance in
Campylobacter jejuni and
Campylobacter coli isolates were investigated. The A2075G mutation in the 23S rRNA target genes was identified in all high-level erythromycin-resistant isolates. A number of amino acid substitutions together with insertions and deletions were identified in the corresponding genes encoding L4 and L22 ribosomal proteins both of resistant and susceptible isolates. Amino acid substitutions identified in the resistant strains were located outside regions known to be altered in these proteins. The efflux pump inhibitor
l-phenylalanine-
l-arginine-β-naphthylamide (PAβN) increased the susceptibility to erythromycin in one of four isolates displaying high-level erythromycin resistance, and reduced the minimal inhibitory concentration displayed by an erythromycin-susceptible
C. coli isolate. The A2075G mutation in the 23S rRNA appeared to be the main contributor to high-level erythromycin resistance in
Campylobacter. Other mutations/amino acid substitutions found in the 50S ribosomal subunit encoding proteins L4 and L22 do not appear to be linked to the high-level erythromycin-resistant phenotype. Active efflux contributes to the intrinsic resistance to erythromycin in
Campylobacter and may contribute to high-level resistance in some isolates.</description><subject>Amino Acid Sequence</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Biological and medical sciences</subject><subject>Campylobacter</subject><subject>Campylobacter coli</subject><subject>Campylobacter coli - drug effects</subject><subject>Campylobacter coli - isolation & purification</subject><subject>Campylobacter coli - physiology</subject><subject>Campylobacter jejuni</subject><subject>Campylobacter jejuni - drug effects</subject><subject>Campylobacter jejuni - isolation & purification</subject><subject>Campylobacter jejuni - physiology</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Efflux</subject><subject>Erythromycin</subject><subject>Erythromycin - pharmacology</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Microbial Sensitivity Tests</subject><subject>Molecular Sequence Data</subject><subject>Pharmacology. Drug treatments</subject><subject>Ribosomal proteins</subject><subject>Ribosomal Proteins - genetics</subject><subject>RNA, Ribosomal, 23S - genetics</subject><issn>0924-8579</issn><issn>1872-7913</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU-P0zAQxS0EYrsLXwGZA9xS7MRO7OOq4p-0Ehc4WxNn0rhK7GI7lfrt10srLTc4jUb6zZuZ9wh5z9mWM95-OmzdAXx2i7Ow39aMyS1TW8b1C7LhqqurTvPmJdkwXYtKyU7fkNuUDoxx2Qj5mtzwtuGqMBtyvPfU-ROm7PaQXfA0jDRPSJcwo11niHRBO4F3aUnUBp-j69fs_J7mQCe3n6oZTzhTjOc8xbCcrfM0YnIpg7dYxOkOluN5Dj3YjPENeTXCnPDttd6RX18-_9x9qx5-fP2-u3-orGR1rnStFEohRiG6WgJqbPtmwAFY2wvbgKitBlBKdKislL3oobQIdmwbCeXPO_LxonuM4fda_jOLSxbnGTyGNZm2a3lTtP8J8k5I2XFWQH0BbQwpRRzNMboF4tlwZp5yMQfzVy7mKRfDlCm5lNl31yVrv-DwPHkNogAfrgAkC_MYi3kuPXNdo7X-c-3uwmHx7uQwmmQdFqMHF9FmMwT3H-c8AmSQtQM</recordid><startdate>2006</startdate><enddate>2006</enddate><creator>Corcoran, Deborah</creator><creator>Quinn, Teresa</creator><creator>Cotter, Leslie</creator><creator>Fanning, Séamus</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>2006</creationdate><title>An investigation of the molecular mechanisms contributing to high-level erythromycin resistance in Campylobacter</title><author>Corcoran, Deborah ; Quinn, Teresa ; Cotter, Leslie ; Fanning, Séamus</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c502t-9288e544f44725ae9e6b3deda06b4c3a42c9aa8847e8c55b4baaa8eacf635a153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amino Acid Sequence</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Biological and medical sciences</topic><topic>Campylobacter</topic><topic>Campylobacter coli</topic><topic>Campylobacter coli - drug effects</topic><topic>Campylobacter coli - isolation & purification</topic><topic>Campylobacter coli - physiology</topic><topic>Campylobacter jejuni</topic><topic>Campylobacter jejuni - drug effects</topic><topic>Campylobacter jejuni - isolation & purification</topic><topic>Campylobacter jejuni - physiology</topic><topic>Drug Resistance, Bacterial - genetics</topic><topic>Efflux</topic><topic>Erythromycin</topic><topic>Erythromycin - pharmacology</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Microbial Sensitivity Tests</topic><topic>Molecular Sequence Data</topic><topic>Pharmacology. Drug treatments</topic><topic>Ribosomal proteins</topic><topic>Ribosomal Proteins - genetics</topic><topic>RNA, Ribosomal, 23S - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Corcoran, Deborah</creatorcontrib><creatorcontrib>Quinn, Teresa</creatorcontrib><creatorcontrib>Cotter, Leslie</creatorcontrib><creatorcontrib>Fanning, Séamus</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of antimicrobial agents</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Corcoran, Deborah</au><au>Quinn, Teresa</au><au>Cotter, Leslie</au><au>Fanning, Séamus</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An investigation of the molecular mechanisms contributing to high-level erythromycin resistance in Campylobacter</atitle><jtitle>International journal of antimicrobial agents</jtitle><addtitle>Int J Antimicrob Agents</addtitle><date>2006</date><risdate>2006</risdate><volume>27</volume><issue>1</issue><spage>40</spage><epage>45</epage><pages>40-45</pages><issn>0924-8579</issn><eissn>1872-7913</eissn><abstract>The molecular mechanisms contributing to high-level erythromycin resistance in
Campylobacter jejuni and
Campylobacter coli isolates were investigated. The A2075G mutation in the 23S rRNA target genes was identified in all high-level erythromycin-resistant isolates. A number of amino acid substitutions together with insertions and deletions were identified in the corresponding genes encoding L4 and L22 ribosomal proteins both of resistant and susceptible isolates. Amino acid substitutions identified in the resistant strains were located outside regions known to be altered in these proteins. The efflux pump inhibitor
l-phenylalanine-
l-arginine-β-naphthylamide (PAβN) increased the susceptibility to erythromycin in one of four isolates displaying high-level erythromycin resistance, and reduced the minimal inhibitory concentration displayed by an erythromycin-susceptible
C. coli isolate. The A2075G mutation in the 23S rRNA appeared to be the main contributor to high-level erythromycin resistance in
Campylobacter. Other mutations/amino acid substitutions found in the 50S ribosomal subunit encoding proteins L4 and L22 do not appear to be linked to the high-level erythromycin-resistant phenotype. Active efflux contributes to the intrinsic resistance to erythromycin in
Campylobacter and may contribute to high-level resistance in some isolates.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>16318913</pmid><doi>10.1016/j.ijantimicag.2005.08.019</doi><tpages>6</tpages></addata></record> |
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| subjects | Amino Acid Sequence Antibiotics. Antiinfectious agents. Antiparasitic agents Biological and medical sciences Campylobacter Campylobacter coli Campylobacter coli - drug effects Campylobacter coli - isolation & purification Campylobacter coli - physiology Campylobacter jejuni Campylobacter jejuni - drug effects Campylobacter jejuni - isolation & purification Campylobacter jejuni - physiology Drug Resistance, Bacterial - genetics Efflux Erythromycin Erythromycin - pharmacology Humans Medical sciences Microbial Sensitivity Tests Molecular Sequence Data Pharmacology. Drug treatments Ribosomal proteins Ribosomal Proteins - genetics RNA, Ribosomal, 23S - genetics |
| title | An investigation of the molecular mechanisms contributing to high-level erythromycin resistance in Campylobacter |
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