Differences in nitrergic innervation of the developing chick cloaca and colorectum
The intrinsic innervation of the developing gut has long been a subject of investigation, but little is known regarding that of the embryonic cloaca. The cloaca, like the rest of the gastrointestinal tract, is intrinsically innervated by the enteric nervous system. Nitrergic neurons and fibres make...
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| Veröffentlicht in: | Pediatric surgery international 2006-01, Vol.22 (1), p.90-94 |
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| description | The intrinsic innervation of the developing gut has long been a subject of investigation, but little is known regarding that of the embryonic cloaca. The cloaca, like the rest of the gastrointestinal tract, is intrinsically innervated by the enteric nervous system. Nitrergic neurons and fibres make up a large part of this system, thus, their distribution provides us with a useful insight into its development. Cloacal and colorectal tissue specimens were removed from chick embryos at embryonic days 11 (E11), E15 and E19. NADPH-diaphorase (NADPH-d) histochemistry was carried out using whole mount tissue preparations. Ganglia density, the number of NADPH-d-positive cells per ganglia in the myenteric plexus and cell size were calculated and statistical analysis was performed to compare both regions of the gut (P |
| doi_str_mv | 10.1007/s00383-005-1590-7 |
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The cloaca, like the rest of the gastrointestinal tract, is intrinsically innervated by the enteric nervous system. Nitrergic neurons and fibres make up a large part of this system, thus, their distribution provides us with a useful insight into its development. Cloacal and colorectal tissue specimens were removed from chick embryos at embryonic days 11 (E11), E15 and E19. NADPH-diaphorase (NADPH-d) histochemistry was carried out using whole mount tissue preparations. Ganglia density, the number of NADPH-d-positive cells per ganglia in the myenteric plexus and cell size were calculated and statistical analysis was performed to compare both regions of the gut (P<0.001). There were significant differences in the ganglia density in the cloaca compared to the colorectum at E11 (P<0.05) and E15 (P<0.01), with the colorectum having a much denser network. In both the cloaca and the colorectum, ganglia density significantly decreased with age (P<0.001), while significant differences were observed in the number of NADPH-d-positive cells per ganglia in both regions through development. Total cell size was similar in both the cloaca and colorectum at each stage and increased in both regions through development, predominantly due to an increase in the cytoplasm. Results reveal striking differences in innervation between the chick embryo cloaca and colorectum. The sparse network of innervation evident within the cloaca in contrast to the dense network within the colorectum emphasizes the individuality of both regions. These results highlight the need for a further in-depth analysis of the enteric nervous system's development within the embryonic cloaca.</description><identifier>ISSN: 0179-0358</identifier><identifier>EISSN: 1437-9813</identifier><identifier>DOI: 10.1007/s00383-005-1590-7</identifier><identifier>PMID: 16328330</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Analysis of Variance ; Animals ; Chick Embryo ; Cloaca - cytology ; Cloaca - embryology ; Cloaca - innervation ; Colon - cytology ; Colon - embryology ; Colon - innervation ; Histocytochemistry ; Myenteric Plexus - embryology ; NADP - metabolism ; Nitrergic Neurons - ultrastructure ; Rectum - cytology ; Rectum - embryology ; Rectum - innervation</subject><ispartof>Pediatric surgery international, 2006-01, Vol.22 (1), p.90-94</ispartof><rights>Springer-Verlag 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c355t-fa7b96bde9b82ff37338f51d757e76b779c1aebb0e3b407deb875f74e21c3eb03</citedby><cites>FETCH-LOGICAL-c355t-fa7b96bde9b82ff37338f51d757e76b779c1aebb0e3b407deb875f74e21c3eb03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16328330$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>O'Donnell, A M</creatorcontrib><creatorcontrib>Bannigan, J</creatorcontrib><creatorcontrib>Puri, P</creatorcontrib><title>Differences in nitrergic innervation of the developing chick cloaca and colorectum</title><title>Pediatric surgery international</title><addtitle>Pediatr Surg Int</addtitle><description>The intrinsic innervation of the developing gut has long been a subject of investigation, but little is known regarding that of the embryonic cloaca. The cloaca, like the rest of the gastrointestinal tract, is intrinsically innervated by the enteric nervous system. Nitrergic neurons and fibres make up a large part of this system, thus, their distribution provides us with a useful insight into its development. Cloacal and colorectal tissue specimens were removed from chick embryos at embryonic days 11 (E11), E15 and E19. NADPH-diaphorase (NADPH-d) histochemistry was carried out using whole mount tissue preparations. Ganglia density, the number of NADPH-d-positive cells per ganglia in the myenteric plexus and cell size were calculated and statistical analysis was performed to compare both regions of the gut (P<0.001). There were significant differences in the ganglia density in the cloaca compared to the colorectum at E11 (P<0.05) and E15 (P<0.01), with the colorectum having a much denser network. In both the cloaca and the colorectum, ganglia density significantly decreased with age (P<0.001), while significant differences were observed in the number of NADPH-d-positive cells per ganglia in both regions through development. Total cell size was similar in both the cloaca and colorectum at each stage and increased in both regions through development, predominantly due to an increase in the cytoplasm. Results reveal striking differences in innervation between the chick embryo cloaca and colorectum. The sparse network of innervation evident within the cloaca in contrast to the dense network within the colorectum emphasizes the individuality of both regions. These results highlight the need for a further in-depth analysis of the enteric nervous system's development within the embryonic cloaca.</description><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Chick Embryo</subject><subject>Cloaca - cytology</subject><subject>Cloaca - embryology</subject><subject>Cloaca - innervation</subject><subject>Colon - cytology</subject><subject>Colon - embryology</subject><subject>Colon - innervation</subject><subject>Histocytochemistry</subject><subject>Myenteric Plexus - embryology</subject><subject>NADP - metabolism</subject><subject>Nitrergic Neurons - ultrastructure</subject><subject>Rectum - cytology</subject><subject>Rectum - embryology</subject><subject>Rectum - innervation</subject><issn>0179-0358</issn><issn>1437-9813</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdkFFrFDEUhYMo7Vr7A3yR4EPfRm_m7szNPEq1tlAQRJ9DkrlpU2eTNZkp-O-dZRcEny4Hzvm4fEK8VfBBAdDHCoAaG4CuUd0ADb0QG7VFagat8KXYgKKhAez0uXhd6xMAaOyHM3Guemw1ImzE988xBC6cPFcZk0xxLlweol9D4vJs55iTzEHOjyxHfuYp72N6kP4x-l_ST9l6K20apc9TLuznZfdGvAp2qnx5uhfi582XH9e3zf23r3fXn-4bj103N8GSG3o38uB0GwISog6dGqkjpt4RDV5Zdg4Y3RZoZKepC7TlVnlkB3ghro7cfcm_F66z2cXqeZps4rxU01OvFFK_Ft__V3zKS0nrb6ZtW4Jew4GmjiVfcq2Fg9mXuLPlj1FgDrbN0bZZbZuDbUPr5t0JvLgdj_8WJ734F3IHexE</recordid><startdate>200601</startdate><enddate>200601</enddate><creator>O'Donnell, A M</creator><creator>Bannigan, J</creator><creator>Puri, P</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>200601</creationdate><title>Differences in nitrergic innervation of the developing chick cloaca and colorectum</title><author>O'Donnell, A M ; Bannigan, J ; Puri, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c355t-fa7b96bde9b82ff37338f51d757e76b779c1aebb0e3b407deb875f74e21c3eb03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Chick Embryo</topic><topic>Cloaca - cytology</topic><topic>Cloaca - embryology</topic><topic>Cloaca - innervation</topic><topic>Colon - cytology</topic><topic>Colon - embryology</topic><topic>Colon - innervation</topic><topic>Histocytochemistry</topic><topic>Myenteric Plexus - embryology</topic><topic>NADP - metabolism</topic><topic>Nitrergic Neurons - ultrastructure</topic><topic>Rectum - cytology</topic><topic>Rectum - embryology</topic><topic>Rectum - innervation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>O'Donnell, A M</creatorcontrib><creatorcontrib>Bannigan, J</creatorcontrib><creatorcontrib>Puri, P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Pediatric surgery international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>O'Donnell, A M</au><au>Bannigan, J</au><au>Puri, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differences in nitrergic innervation of the developing chick cloaca and colorectum</atitle><jtitle>Pediatric surgery international</jtitle><addtitle>Pediatr Surg Int</addtitle><date>2006-01</date><risdate>2006</risdate><volume>22</volume><issue>1</issue><spage>90</spage><epage>94</epage><pages>90-94</pages><issn>0179-0358</issn><eissn>1437-9813</eissn><abstract>The intrinsic innervation of the developing gut has long been a subject of investigation, but little is known regarding that of the embryonic cloaca. The cloaca, like the rest of the gastrointestinal tract, is intrinsically innervated by the enteric nervous system. Nitrergic neurons and fibres make up a large part of this system, thus, their distribution provides us with a useful insight into its development. Cloacal and colorectal tissue specimens were removed from chick embryos at embryonic days 11 (E11), E15 and E19. NADPH-diaphorase (NADPH-d) histochemistry was carried out using whole mount tissue preparations. Ganglia density, the number of NADPH-d-positive cells per ganglia in the myenteric plexus and cell size were calculated and statistical analysis was performed to compare both regions of the gut (P<0.001). There were significant differences in the ganglia density in the cloaca compared to the colorectum at E11 (P<0.05) and E15 (P<0.01), with the colorectum having a much denser network. In both the cloaca and the colorectum, ganglia density significantly decreased with age (P<0.001), while significant differences were observed in the number of NADPH-d-positive cells per ganglia in both regions through development. Total cell size was similar in both the cloaca and colorectum at each stage and increased in both regions through development, predominantly due to an increase in the cytoplasm. Results reveal striking differences in innervation between the chick embryo cloaca and colorectum. The sparse network of innervation evident within the cloaca in contrast to the dense network within the colorectum emphasizes the individuality of both regions. These results highlight the need for a further in-depth analysis of the enteric nervous system's development within the embryonic cloaca.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>16328330</pmid><doi>10.1007/s00383-005-1590-7</doi><tpages>5</tpages></addata></record> |
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| subjects | Analysis of Variance Animals Chick Embryo Cloaca - cytology Cloaca - embryology Cloaca - innervation Colon - cytology Colon - embryology Colon - innervation Histocytochemistry Myenteric Plexus - embryology NADP - metabolism Nitrergic Neurons - ultrastructure Rectum - cytology Rectum - embryology Rectum - innervation |
| title | Differences in nitrergic innervation of the developing chick cloaca and colorectum |
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